Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001141

Physical Location & Seq

Genomic Coordinates Reverse (-)

26865734 .. 26867752

Gene Structure 2019 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1196200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGATCGTACGGTCCAGCCATCGCCGGAAATGCAGCAATCCGTGGCAGCCGTGGAGCAAATCGTGGGCTACGATTTCAGGAACAAGAGGCTTCTAGAAGAAGCTCTGACCCACTCCTCTTACTCCGAATCGCAGTCGTACCAGCGCCTCGAATTCGTCGGCGACGCTGCTCTCGGCCTCGCCCTCAGCAACTACTTCTTCCTTGCTTATCCTGGGGTCGAACCCGGCACTCTCTCTCTGCTGCGCTCCGCCAACGTCAGCACCGAGAAGCTCGCACGTGTGGCTGTCCGGCACGGCCTTTACTGCCACCTCCGCCACAATGCACCTGCTCTCCAGGATAAGGTTAGGGAGTTTACTGAAGCTGTCACTGGAGAAGACGAGACGCCGCTTGTATATCGTGGTTCTGTGAGAGCTCCCAAGGTTCTTGCAGATATGGTGGAGTCTGTGGCAGCAGCAGTGTATGTTGACTTGAATTTTGATCTGGAAAAATTATGGATGAAATTTAGGCGCCTTTTGGAGCCTATAGTTACTCTTGAAGAAGTGCAAGGTCAACCTCAACCTGTTACAATGTTGTTTGAGCTTTGCCAGAAGCACGGGAAACATGTTGATATCAAGCATTGGAGAGATGAGGCCAAAAGTATTGCTAGTGTCTATGTTGATGGCGTGTTTGTTGCCTCGGGTTCTTCTGAGCAAAAGGAGATTGCCAAGCTTGATGCTGCTAAGATAGCTCTTTTAAAGTTGTCGAAATCGTTGGGGGTAAGTGGCAGGTCGACGGAATTTATTGCGGGAATTGATGGAAAGTTTCAAATTGAAGAAGCAAAACAGAAGTTGCATGAGCTTTGTGACAAGAAGAAGTGGCCTAAACCAATTTACACCATTGAGAAAGATGAAGGTCCTTCACATGAGAAGAGATTTGTATCCTCAGTCAAAATTGCAACTGCGGATGGTGTACTATGCATAACGGGAGATGAAAGGTCGAGAATAAAAGATGCGGACAACTCTGCAGCTTCGTCAATGATCTGTGCTTTACAAGAATCTGGTTATATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DND1_DSRM Ribonuclease_3 dsrm

Protein Analysis

350

Amino Acids

38.79

Weight (kDa)

6.04

Isoelectric Point (pI)

42.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonucleas_3_3	PF14622	28 - 159	1.2e-16	Ribonuclease-III-like
Ribonuclease_3	PF00636	48 - 156	2.4e-20	Ribonuclease III domain
dsrm	PF00035	187 - 247	3.4e-07	Double-stranded RNA binding motif
DND1_DSRM	PF14709	272 - 344	3.2e-14	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	273 - 341	4e-13	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001141)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20420
fragaria_vesca	FvH4_3g23250
malus_domestica	MD03G1196200.v1.1 MD11G1212500.v1.1
prunus_persica	Prupe.4G219200_v2.0.a1 Prupe.4G219200_v2.0.a1 Prupe.4G219400_v2.0.a1 Prupe.4G219400_v2.0.a1
pyrus_communis	pycom03g14830 pycom11g18750
rosa_chinensis	RchiOBHm_Chr5g0040511 RchiOBHm_Chr5g0040931 RchiOBHm_Chr5g0040941
rosa_laevigata	RLG00000033986 RLG00000034014 RLG00000034016 RLG00000034026
rosa_multiflora	Rmu_co8051550.1_g000001 Rmu_co8392905.1_g000001 Rmu_sc0002475.1_g000019 Rmu_sc0002848.1_g000007 Rmu_sc0006651.1_g000007 Rmu_sc0006651.1_g000008 Rmu_sc0008620.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039580 Rroxscaffold_1G00039750 Rroxscaffold_1G00039760 Rroxscaffold_1G00040130 Rroxscaffold_1G00040850
rosa_rugosa	Rorug02G0350900 Rorug02G0350900 Rorug02G0351000 Rorug05G0185500 Rorug05G0188700 Rorug05G0188900 Rorug05G0188900
rosa_samantha	Rh2AG398700 Rh2BG409100 Rh2CG385500 Rh2CG540800 Rh2DG418900 Rh5AG272400 Rh5AG274300 Rh5AG274400 Rh5AG344200 Rh5BG276400 Rh5BG279300 Rh5BG279400 Rh5CG308900 Rh5CG311400 Rh5CG311600 Rh5DG284900 Rh5DG287500
rosa_wichuraiana	Rw2G032480 Rw5G025510 Rw5G025770 Rw5G025780 Rw5G025790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	334
Acc36I	ACCTGC	2 cut(s)	334, 756
AccB1I	GGYRCC	1 cut(s)	507
AccI	GTMKAC	1 cut(s)	770
AciI	CCGC	6 cut(s)	249, 313, 386, 785, 941, 992
AclWI	GGATC	1 cut(s)	12
AcsI	RAATTY	4 cut(s)	152, 472, 500, 776
AcuI	CTGAAG	1 cut(s)	378
AcvI	CACGTG	1 cut(s)	278
AcyI	GRCGYC	2 cut(s)	383, 508
AfaI	GTAC	3 cut(s)	10, 140, 951
AfiI	CCNNNNNNNGG	1 cut(s)	340
AflIII	ACRYGT	2 cut(s)	277, 601
AgsI	TTSAA	4 cut(s)	472, 536, 806, 812
AjnI	CCWGG	2 cut(s)	211, 333
AluBI	AGCT	9 cut(s)	104, 271, 362, 413, 580, 709, 728, 838, 1007
AluI	AGCT	9 cut(s)	104, 271, 362, 413, 580, 709, 728, 838, 1007
Alw21I	GWGCWC	1 cut(s)	415
Alw26I	GTCTC	1 cut(s)	374
AlwI	GGATC	1 cut(s)	12
Ama87I	CYCGRG	1 cut(s)	676
AoxI	GGCC	4 cut(s)	175, 295, 630, 857
ApeKI	GCWGC	8 cut(s)	34, 47, 167, 241, 449, 452, 716, 1004
ApoI	RAATTY	4 cut(s)	152, 472, 500, 776
ArsI	GACNNNNNNTTYG	2 cut(s)	458, 490
AspLEI	GCGC	3 cut(s)	147, 246, 510
AspS9I	GGNCC	2 cut(s)	13, 893
AsuC2I	CCSGG	1 cut(s)	225
AvaI	CYCGRG	1 cut(s)	676
AvaII	GGWCC	2 cut(s)	13, 893
BanI	GGYRCC	1 cut(s)	507
BanII	GRGCYC	1 cut(s)	415
BbrPI	CACGTG	1 cut(s)	278
BbsI	GAAGAC	1 cut(s)	381
Bbv12I	GWGCWC	1 cut(s)	415
BbvCI	CCTCAGC	1 cut(s)	185
BbvI	GCAGC	8 cut(s)	46, 59, 154, 228, 461, 464, 703, 1016
BccI	CCATC	4 cut(s)	28, 653, 788, 938
BceAI	ACGGC	2 cut(s)	35, 310
BciT130I	CCWGG	2 cut(s)	213, 335
BciVI	GTATCC	1 cut(s)	928
BcnI	CCSGG	1 cut(s)	225
BcoDI	GTCTC	1 cut(s)	374
BfaI	CTAG	2 cut(s)	95, 645
BfmI	CTRYAG	2 cut(s)	522, 1002
BfoI	RGCGCY	2 cut(s)	148, 511
BfuAI	ACCTGC	2 cut(s)	334, 756
BfuI	GTATCC	1 cut(s)	928
BisI	GCNGC	9 cut(s)	35, 48, 168, 242, 386, 450, 453, 717, 1005
BlsI	GCNGC	9 cut(s)	36, 49, 169, 243, 387, 451, 454, 718, 1006
Bme1390I	CCNGG	3 cut(s)	213, 225, 335
Bme18I	GGWCC	2 cut(s)	13, 893
BmeT110I	CYCGRG	1 cut(s)	676
BmgT120I	GGNCC	2 cut(s)	13, 893
BmiI	GGNNCC	2 cut(s)	509, 519
BmrFI	CCNGG	3 cut(s)	213, 225, 335
BmsI	GCATC	2 cut(s)	703, 979
BpiI	GAAGAC	1 cut(s)	381
BpmI	CTGGAG	2 cut(s)	317, 390
Bpu10I	CCTNAGC	1 cut(s)	185
BpuMI	CCSGG	1 cut(s)	225
BsaAI	YACGTR	1 cut(s)	278
BsaBI	GATNNNNATC	1 cut(s)	916
BsaHI	GRCGYC	2 cut(s)	383, 508
BsaJI	CCNNGG	5 cut(s)	42, 51, 212, 417, 675
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	1 cut(s)	340
Bse1I	ACTGG	1 cut(s)	373
Bse8I	GATNNNNATC	1 cut(s)	916
BseBI	CCWGG	2 cut(s)	213, 335
BseDI	CCNNGG	5 cut(s)	42, 51, 212, 417, 675
BseGI	GGATG	2 cut(s)	501, 949
BseJI	GATNNNNATC	1 cut(s)	916
BseLI	CCNNNNNNNGG	1 cut(s)	340
BseMII	CTCAG	3 cut(s)	199, 678, 936
BseNI	ACTGG	1 cut(s)	373
BseRI	GAGGAG	1 cut(s)	106
BseXI	GCAGC	8 cut(s)	46, 59, 154, 228, 461, 464, 703, 1016
BshFI	GGCC	4 cut(s)	177, 297, 632, 859
BshNI	GGYRCC	1 cut(s)	507
BsiHKAI	GWGCWC	1 cut(s)	415
BsiHKCI	CYCGRG	1 cut(s)	676
BsiSI	CCGG	3 cut(s)	26, 225, 289
BsiWI	CGTACG	1 cut(s)	8
BslI	CCNNNNNNNGG	1 cut(s)	340
BsmAI	GTCTC	1 cut(s)	374
BsmBI	CGTCTC	1 cut(s)	374
BsnI	GGCC	4 cut(s)	177, 297, 632, 859
BsoBI	CYCGRG	1 cut(s)	676
Bsp1286I	GDGCHC	1 cut(s)	415
Bsp143I	GATC	3 cut(s)	4, 478, 1017
BspACI	CCGC	6 cut(s)	249, 313, 386, 785, 941, 992
BspANI	GGCC	4 cut(s)	177, 297, 632, 859
BspCNI	CTCAG	3 cut(s)	198, 679, 935
BspLI	GGNNCC	2 cut(s)	509, 519
BspMAI	CTGCAG	1 cut(s)	1006
BspMI	ACCTGC	2 cut(s)	334, 756
BspPI	GGATC	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	507
BsrI	ACTGG	1 cut(s)	373
BssECI	CCNNGG	5 cut(s)	42, 51, 212, 417, 675
BssMI	GATC	3 cut(s)	4, 478, 1017
BssNI	GRCGYC	2 cut(s)	383, 508
BssT1I	CCWWGG	1 cut(s)	417
Bst2UI	CCWGG	2 cut(s)	213, 335
Bst4CI	ACNGT	1 cut(s)	13
Bst6I	CTCTTC	1 cut(s)	902
BstACI	GRCGYC	2 cut(s)	383, 508
BstBAI	YACGTR	1 cut(s)	278
BstC8I	GCNNGC	1 cut(s)	273
BstDEI	CTNAG	4 cut(s)	185, 687, 720, 922
BstDSI	CCRYGG	2 cut(s)	42, 51
BstF5I	GGATG	2 cut(s)	501, 949
BstH2I	RGCGCY	2 cut(s)	148, 511
BstHHI	GCGC	3 cut(s)	147, 246, 510
BstKTI	GATC	3 cut(s)	7, 481, 1020
BstMAI	GTCTC	1 cut(s)	374
BstMBI	GATC	3 cut(s)	4, 478, 1017
BstMWI	GCNNNNNNNGC	5 cut(s)	31, 281, 303, 312, 725
BstNI	CCWGG	2 cut(s)	213, 335
BstNSI	RCATGY	1 cut(s)	605
BstSCI	CCNGG	3 cut(s)	211, 223, 333
BstSFI	CTRYAG	2 cut(s)	522, 1002
BstV1I	GCAGC	8 cut(s)	46, 59, 154, 228, 461, 464, 703, 1016
BstV2I	GAAGAC	1 cut(s)	381
BsuI	GTATCC	1 cut(s)	928
BsuRI	GGCC	4 cut(s)	177, 297, 632, 859
BtgI	CCRYGG	2 cut(s)	42, 51
BtgZI	GCGATG	1 cut(s)	6
BtsCI	GGATG	2 cut(s)	501, 949
BtsI	GCAGTG	1 cut(s)	462
BtsIMutI	CAGTG	2 cut(s)	366, 462
BveI	ACCTGC	2 cut(s)	334, 756
Cac8I	GCNNGC	1 cut(s)	273
CfoI	GCGC	3 cut(s)	147, 246, 510
Cfr13I	GGNCC	2 cut(s)	13, 893
CseI	GACGC	2 cut(s)	173, 391
Csp6I	GTAC	3 cut(s)	9, 139, 950
CviAII	CATG	3 cut(s)	602, 833, 902
CviQI	GTAC	3 cut(s)	9, 139, 950
DdeI	CTNAG	4 cut(s)	185, 687, 720, 922
DinI	GGCGCC	1 cut(s)	509
DpnI	GATC	3 cut(s)	6, 480, 1019
DpnII	GATC	3 cut(s)	4, 478, 1017
DraI	TTTAAA	1 cut(s)	735
Eam1104I	CTCTTC	1 cut(s)	902
EarI	CTCTTC	1 cut(s)	902
EciI	GGCGGA	2 cut(s)	238, 302
Ecl136II	GAGCTC	1 cut(s)	413
Eco130I	CCWWGG	1 cut(s)	417
Eco24I	GRGCYC	1 cut(s)	415
Eco32I	GATATC	1 cut(s)	610
Eco47I	GGWCC	2 cut(s)	13, 893
Eco53kI	GAGCTC	1 cut(s)	413
Eco57I	CTGAAG	1 cut(s)	378
Eco72I	CACGTG	1 cut(s)	278
Eco88I	CYCGRG	1 cut(s)	676
EcoICRI	GAGCTC	1 cut(s)	413
EcoO109I	RGGNCCY	1 cut(s)	893
EcoRI	GAATTC	1 cut(s)	152
EcoRII	CCWGG	2 cut(s)	211, 333
EcoRV	GATATC	1 cut(s)	610
EcoT14I	CCWWGG	1 cut(s)	417
EcoT22I	ATGCAT	1 cut(s)	959
EcoT38I	GRGCYC	1 cut(s)	415
EgeI	GGCGCC	1 cut(s)	509
EheI	GGCGCC	1 cut(s)	509
ErhI	CCWWGG	1 cut(s)	417
Esp3I	CGTCTC	1 cut(s)	374
FaeI	CATG	3 cut(s)	605, 836, 905
FatI	CATG	3 cut(s)	601, 832, 901
FauI	CCCGC	1 cut(s)	778
FblI	GTMKAC	1 cut(s)	770
Fnu4HI	GCNGC	9 cut(s)	35, 48, 168, 242, 386, 450, 453, 717, 1005
FokI	GGATG	2 cut(s)	508, 956
FriOI	GRGCYC	1 cut(s)	415
Fsp4HI	GCNGC	9 cut(s)	35, 48, 168, 242, 386, 450, 453, 717, 1005
FspBI	CTAG	2 cut(s)	95, 645
GlaI	GCGC	3 cut(s)	146, 245, 509
GluI	GCNGC	9 cut(s)	35, 48, 168, 242, 386, 450, 453, 717, 1005
GsuI	CTGGAG	2 cut(s)	317, 390
HaeII	RGCGCY	2 cut(s)	148, 511
HaeIII	GGCC	4 cut(s)	177, 297, 632, 859
HapII	CCGG	3 cut(s)	26, 225, 289
HgaI	GACGC	2 cut(s)	173, 391
HhaI	GCGC	3 cut(s)	147, 246, 510
Hin1I	GRCGYC	2 cut(s)	383, 508
Hin1II	CATG	3 cut(s)	605, 836, 905
Hin6I	GCGC	3 cut(s)	145, 244, 508
HinP1I	GCGC	3 cut(s)	145, 244, 508
HincII	GTYRAC	3 cut(s)	466, 551, 771
HindII	GTYRAC	3 cut(s)	466, 551, 771
HindIII	AAGCTT	1 cut(s)	707
HinfI	GANTC	3 cut(s)	128, 440, 1034
HpaII	CCGG	3 cut(s)	26, 225, 289
Hpy166II	GTNNAC	5 cut(s)	354, 466, 551, 771, 950
Hpy188I	TCNGA	3 cut(s)	108, 127, 688
Hpy188III	TCNNGA	5 cut(s)	79, 95, 482, 533, 978
Hpy8I	GTNNAC	5 cut(s)	354, 466, 551, 771, 950
Hpy99I	CGWCG	3 cut(s)	161, 167, 775
HpyAV	CCTTC	2 cut(s)	884, 906
HpyCH4III	ACNGT	1 cut(s)	13
HpyCH4IV	ACGT	2 cut(s)	255, 277
HpyCH4V	TGCA	8 cut(s)	34, 323, 428, 544, 832, 935, 957, 1004
HpyF10VI	GCNNNNNNNGC	5 cut(s)	31, 281, 303, 312, 725
HpyF3I	CTNAG	4 cut(s)	185, 687, 720, 922
HpySE526I	ACGT	2 cut(s)	255, 277
Hsp92I	GRCGYC	2 cut(s)	383, 508
Hsp92II	CATG	3 cut(s)	605, 836, 905
HspAI	GCGC	3 cut(s)	145, 244, 508
KasI	GGCGCC	1 cut(s)	507
Kzo9I	GATC	3 cut(s)	4, 478, 1017
LmnI	GCTCC	4 cut(s)	55, 251, 418, 517
Lsp1109I	GCAGC	8 cut(s)	46, 59, 154, 228, 461, 464, 703, 1016
LweI	GCATC	2 cut(s)	703, 979
MaeI	CTAG	2 cut(s)	95, 645
MaeII	ACGT	2 cut(s)	255, 277
MaeIII	GTNAC	4 cut(s)	364, 526, 562, 842
MalI	GATC	3 cut(s)	6, 480, 1019
MboI	GATC	3 cut(s)	4, 478, 1017
MboII	GAAGA	8 cut(s)	110, 190, 386, 548, 675, 824, 862, 919
MhlI	GDGCHC	1 cut(s)	415
MluCI	AATT	9 cut(s)	152, 472, 488, 500, 776, 789, 807, 867, 930
Mly113I	GGCGCC	1 cut(s)	508
MlyI	GAGTC	1 cut(s)	449
Mph1103I	ATGCAT	1 cut(s)	959
MseI	TTAA	1 cut(s)	734
MspI	CCGG	3 cut(s)	26, 225, 289
MspR9I	CCNGG	3 cut(s)	213, 225, 335
MvaI	CCWGG	2 cut(s)	213, 335
MwoI	GCNNNNNNNGC	5 cut(s)	31, 281, 303, 312, 725
NarI	GGCGCC	1 cut(s)	508
NciI	CCSGG	1 cut(s)	225
NdeII	GATC	3 cut(s)	4, 478, 1017
NlaIII	CATG	3 cut(s)	605, 836, 905
NlaIV	GGNNCC	2 cut(s)	509, 519
NmeAIII	GCCGAG	1 cut(s)	153
NmuCI	GTSAC	2 cut(s)	364, 842
NsiI	ATGCAT	1 cut(s)	959
NspI	RCATGY	1 cut(s)	605
PaqCI	CACCTGC	1 cut(s)	334
PciI	ACATGT	1 cut(s)	601
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 261
PfeI	GAWTC	2 cut(s)	128, 1034
Pfl23II	CGTACG	1 cut(s)	8
PfoI	TCCNGGA	1 cut(s)	333
PkrI	GCNGC	9 cut(s)	36, 49, 169, 243, 387, 451, 454, 718, 1006
PleI	GAGTC	1 cut(s)	448
PluTI	GGCGCC	1 cut(s)	511
PmaCI	CACGTG	1 cut(s)	278
PmlI	CACGTG	1 cut(s)	278
PpsI	GAGTC	1 cut(s)	448
Ppu21I	YACGTR	1 cut(s)	278
PpuMI	RGGWCCY	1 cut(s)	893
PscI	ACATGT	1 cut(s)	601
Psp124BI	GAGCTC	1 cut(s)	415
Psp5II	RGGWCCY	1 cut(s)	893
Psp6I	CCWGG	2 cut(s)	211, 333
PspCI	CACGTG	1 cut(s)	278
PspGI	CCWGG	2 cut(s)	211, 333
PspLI	CGTACG	1 cut(s)	8
PspN4I	GGNNCC	2 cut(s)	509, 519
PspPI	GGNCC	2 cut(s)	13, 893
PspPPI	RGGWCCY	1 cut(s)	893
PstI	CTGCAG	1 cut(s)	1006
RsaI	GTAC	3 cut(s)	10, 140, 951
RsaNI	GTAC	3 cut(s)	9, 139, 950
SacI	GAGCTC	1 cut(s)	415
SalI	GTCGAC	1 cut(s)	769
SaqAI	TTAA	1 cut(s)	734
SatI	GCNGC	9 cut(s)	35, 48, 168, 242, 386, 450, 453, 717, 1005
Sau3AI	GATC	3 cut(s)	4, 478, 1017
Sau96I	GGNCC	2 cut(s)	13, 893
SchI	GAGTC	1 cut(s)	449
ScrFI	CCNGG	3 cut(s)	213, 225, 335
SduI	GDGCHC	1 cut(s)	415
SfaNI	GCATC	2 cut(s)	703, 979
SfcI	CTRYAG	2 cut(s)	522, 1002
SfoI	GGCGCC	1 cut(s)	509
SinI	GGWCC	2 cut(s)	13, 893
Sse9I	AATT	9 cut(s)	152, 472, 488, 500, 776, 789, 807, 867, 930
SsiI	CCGC	6 cut(s)	249, 313, 386, 785, 941, 992
SspDI	GGCGCC	1 cut(s)	507
SspMI	CTAG	2 cut(s)	95, 645
SstI	GAGCTC	1 cut(s)	415
StyD4I	CCNGG	3 cut(s)	211, 223, 333
StyI	CCWWGG	1 cut(s)	417
TaaI	ACNGT	1 cut(s)	13
TaiI	ACGT	2 cut(s)	258, 280
TaqI	TCGA	5 cut(s)	150, 219, 743, 770, 977
TasI	AATT	9 cut(s)	152, 472, 488, 500, 776, 789, 807, 867, 930
TatI	WGTACW	1 cut(s)	949
TauI	GCSGC	1 cut(s)	388
TfiI	GAWTC	2 cut(s)	128, 1034
Tru1I	TTAA	1 cut(s)	734
Tru9I	TTAA	1 cut(s)	734
TscAI	CASTG	2 cut(s)	373, 462
TseFI	GTSAC	2 cut(s)	364, 842
TseI	GCWGC	8 cut(s)	34, 47, 167, 241, 449, 452, 716, 1004
Tsp45I	GTSAC	2 cut(s)	364, 842
TspDTI	ATGAA	3 cut(s)	512, 903, 984
TspGWI	ACGGA	2 cut(s)	31, 788
TspRI	CASTG	2 cut(s)	373, 462
VpaK11BI	GGWCC	2 cut(s)	13, 893
XapI	RAATTY	4 cut(s)	152, 472, 500, 776
XbaI	TCTAGA	1 cut(s)	94
XceI	RCATGY	1 cut(s)	605
XmiI	GTMKAC	1 cut(s)	770
XspI	CTAG	2 cut(s)	95, 645
Zsp2I	ATGCAT	1 cut(s)	959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.