MD03G1234700.v1.1

ABC transporter I family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001882
Physical Location & Seq
Reverse (-)
32045035 .. 32045583
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1234700.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGTGTGAATCCAAACTCCGAGCCGTCGATCGAGATCAACGGCCTAAAATTCACCTACCCAGGAATCAACGGCCACCCGCCGCCGGGCTCCAAGCCGCTGATCAAGTACTTCAATCTCACCTTTAACGCCAGCGACCGATGCCTCCTCGTCGGATCCAACGGCGCGGGGAAAACCACGATCTTGAAGATCCTGGGCGGCAAGCACATGGTCGAGCCCCACATGGTTCGCGTGCTAGGAAGGTCGGCGTTTCACGACACCGCCTTGACCGGATCCGGCGACCTCTCGTATCTCGGCGGAGAGTGGCGGCGAGATGTCGCATTCGCCGGGTTTGATGTCCCGATTCAAATGGACGTTTCGGCTGATAAGTTGATCAATGGGGTTGCGGGGATCGACCCACAAAGGAGAGCAGAGCTGATAAAGGTTTTGGATATTGATCTTTCATGGCGGTTGCACAAGGTGTCCGATGGTCAGAGAAGACGAGTGCAAATCTGTATGGGTCTGCTCAAGCCATACAAGGTACTCAATTTCCATGTTTCACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
ABC_tran

Protein Analysis

183

Amino Acids

20.01

Weight (kDa)

9.48

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 37 - 175 2.7e-11 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 167, 231
AclWI GGATC 6 cut(s) 150, 163, 184, 267, 280, 398
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 1 cut(s) 50
AdeI CACNNNGTG 1 cut(s) 460
AfaI GTAC 2 cut(s) 110, 522
AfiI CCNNNNNNNGG 2 cut(s) 85, 86
AgsI TTSAA 3 cut(s) 115, 187, 347
AjnI CCWGG 2 cut(s) 61, 192
AluBI AGCT 1 cut(s) 415
AluI AGCT 1 cut(s) 415
AlwI GGATC 6 cut(s) 150, 163, 184, 267, 280, 398
AoxI GGCC 2 cut(s) 43, 73
ApoI RAATTY 1 cut(s) 50
AspLEI GCGC 1 cut(s) 167
AsuC2I CCSGG 2 cut(s) 87, 328
AsuHPI GGTGA 2 cut(s) 46, 112
BamHI GGATCC 2 cut(s) 155, 272
BanII GRGCYC 2 cut(s) 92, 219
BbsI GAAGAC 1 cut(s) 484
BccI CCATC 1 cut(s) 461
BceAI ACGGC 4 cut(s) 10, 58, 88, 178
BciT130I CCWGG 2 cut(s) 63, 194
BclI TGATCA 2 cut(s) 102, 372
BcnI CCSGG 2 cut(s) 87, 328
BfaI CTAG 1 cut(s) 236
BisI GCNGC 4 cut(s) 83, 98, 199, 308
BlsI GCNGC 4 cut(s) 84, 99, 200, 309
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 4 cut(s) 63, 87, 194, 328
BmiI GGNNCC 3 cut(s) 91, 157, 274
BmrFI CCNGG 4 cut(s) 63, 87, 194, 328
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 87, 328
BsaBI GATNNNNATC 2 cut(s) 35, 435
BsaJI CCNNGG 2 cut(s) 61, 193
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 86
Bse8I GATNNNNATC 2 cut(s) 35, 435
BseBI CCWGG 2 cut(s) 63, 194
BseDI CCNNGG 2 cut(s) 61, 193
BseJI GATNNNNATC 2 cut(s) 35, 435
BseLI CCNNNNNNNGG 2 cut(s) 85, 86
BseRI GAGGAG 1 cut(s) 137
Bsh1236I CGCG 2 cut(s) 167, 231
Bsh1285I CGRYCG 2 cut(s) 33, 139
BshFI GGCC 2 cut(s) 45, 75
BsiEI CGRYCG 2 cut(s) 33, 139
BsiSI CCGG 4 cut(s) 86, 270, 276, 327
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 2 cut(s) 85, 86
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 2 cut(s) 45, 75
Bsp1286I GDGCHC 2 cut(s) 92, 219
BspANI GGCC 2 cut(s) 45, 75
BspFNI CGCG 2 cut(s) 167, 231
BspLI GGNNCC 3 cut(s) 91, 157, 274
BspPI GGATC 6 cut(s) 150, 163, 184, 267, 280, 398
BssECI CCNNGG 2 cut(s) 61, 193
Bst2UI CCWGG 2 cut(s) 63, 194
Bst4CI ACNGT 1 cut(s) 543
BstC8I GCNNGC 3 cut(s) 133, 203, 233
BstFNI CGCG 2 cut(s) 167, 231
BstHHI GCGC 1 cut(s) 167
BstMCI CGRYCG 2 cut(s) 33, 139
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNI CCWGG 2 cut(s) 63, 194
BstSCI CCNGG 4 cut(s) 61, 85, 192, 326
BstUI CGCG 2 cut(s) 167, 231
BstV2I GAAGAC 1 cut(s) 484
BstX2I RGATCY 3 cut(s) 155, 189, 272
BstYI RGATCY 3 cut(s) 155, 189, 272
BsuRI GGCC 2 cut(s) 45, 75
Cac8I GCNNGC 3 cut(s) 133, 203, 233
CfoI GCGC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 109, 521
CviAII CATG 4 cut(s) 208, 223, 444, 533
CviJI RGCY 9 cut(s) 25, 45, 75, 90, 97, 217, 362, 415, 511
CviKI_1 RGCY 9 cut(s) 25, 45, 75, 90, 97, 217, 362, 415, 511
CviQI GTAC 2 cut(s) 109, 521
DraIII CACNNNGTG 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 73
EciI GGCGGA 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 92, 219
EcoRII CCWGG 2 cut(s) 61, 192
EcoT38I GRGCYC 2 cut(s) 92, 219
FaeI CATG 4 cut(s) 211, 226, 447, 536
FaiI YATR 6 cut(s) 209, 224, 445, 497, 514, 534
FaqI GGGAC 1 cut(s) 323
FatI CATG 4 cut(s) 207, 222, 443, 532
FauI CCCGC 3 cut(s) 87, 160, 379
FbaI TGATCA 2 cut(s) 102, 372
Fnu4HI GCNGC 4 cut(s) 83, 98, 199, 308
FriOI GRGCYC 2 cut(s) 92, 219
Fsp4HI GCNGC 4 cut(s) 83, 98, 199, 308
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 166
GluI GCNGC 4 cut(s) 83, 98, 199, 308
HaeIII GGCC 2 cut(s) 45, 75
HapII CCGG 4 cut(s) 86, 270, 276, 327
HhaI GCGC 1 cut(s) 167
Hin1II CATG 4 cut(s) 211, 226, 447, 536
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HinfI GANTC 3 cut(s) 10, 66, 343
HpaII CCGG 4 cut(s) 86, 270, 276, 327
HphI GGTGA 2 cut(s) 46, 112
Hpy188I TCNGA 4 cut(s) 22, 155, 466, 474
Hpy188III TCNNGA 4 cut(s) 34, 184, 254, 340
Hpy99I CGWCG 2 cut(s) 31, 155
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 454, 487
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 4 cut(s) 211, 226, 447, 536
HspAI GCGC 1 cut(s) 165
Ksp22I TGATCA 2 cut(s) 102, 372
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 9 cut(s) 48, 75, 99, 145, 179, 206, 283, 289, 340
LweI GCATC 1 cut(s) 131
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 354
MboII GAAGA 2 cut(s) 199, 489
MflI RGATCY 3 cut(s) 155, 189, 272
MhlI GDGCHC 2 cut(s) 92, 219
MluCI AATT 2 cut(s) 50, 526
MmeI TCCRAC 2 cut(s) 133, 183
MnlI CCTC 3 cut(s) 155, 158, 293
MseI TTAA 1 cut(s) 126
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 4 cut(s) 86, 270, 276, 327
MspR9I CCNGG 4 cut(s) 63, 87, 194, 328
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 2 cut(s) 63, 194
MvnI CGCG 2 cut(s) 167, 231
MwoI GCNNNNNNNGC 1 cut(s) 141
NciI CCSGG 2 cut(s) 87, 328
NlaIII CATG 4 cut(s) 211, 226, 447, 536
NlaIV GGNNCC 3 cut(s) 91, 157, 274
NmeAIII GCCGAG 1 cut(s) 273
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 3 cut(s) 10, 66, 343
PkrI GCNGC 4 cut(s) 84, 99, 200, 309
Ple19I CGATCG 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 61, 192
PspGI CCWGG 2 cut(s) 61, 192
PspN4I GGNNCC 3 cut(s) 91, 157, 274
PsuI RGATCY 3 cut(s) 155, 189, 272
PvuI CGATCG 1 cut(s) 33
RsaI GTAC 2 cut(s) 110, 522
RsaNI GTAC 2 cut(s) 109, 521
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 4 cut(s) 83, 98, 199, 308
ScaI AGTACT 1 cut(s) 110
ScrFI CCNGG 4 cut(s) 63, 87, 194, 328
SduI GDGCHC 2 cut(s) 92, 219
SetI ASST 9 cut(s) 59, 125, 245, 285, 357, 417, 426, 462, 522
SfaNI GCATC 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 2 cut(s) 50, 526
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 4 cut(s) 61, 85, 192, 326
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 1 cut(s) 357
TaqI TCGA 4 cut(s) 29, 33, 213, 393
TaqII GACCGA 1 cut(s) 153
TasI AATT 2 cut(s) 50, 526
TatI WGTACW 1 cut(s) 108
TauI GCSGC 4 cut(s) 85, 100, 201, 310
TfiI GAWTC 3 cut(s) 10, 66, 343
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TspDTI ATGAA 1 cut(s) 432
XapI RAATTY 1 cut(s) 50
XspI CTAG 1 cut(s) 236
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.