MD05G1219400.v1.1

glycosyltransferase 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001277
Physical Location & Seq
Reverse (-)
34981751 .. 34983079
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1219400.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGGTTCCTCAGAATTCTCTCTCTTCCAACCATCACCAAAACGCTCCGTTCTCCACAAAGCCCCTTCCCTAATAGCAGATGGCTTTCTCTTCGTCGGAGGTGCATCGTTGGCTCTCTCTGTTGTCTGGGCTCTCCTCACCTTCATAAACCCCACCACCATAAGCTTCGACAACATCATCGCATGGAACGCCGGGCCATGCGGACCCGACTTGCGCTCTGACCCGCCGGAACCCACGTTCTACGACGACGAAAATCTCAGCTACGCGTTTGGGGAACCAGTGAAGGATTGGGACTCCAAGCGCAGAGAGTGGCTGAAGCTCCACCCTTCATTCGCTGCCGGAGCCGCCGTGGACAACAGAGTTCTTCTCGTGACTGGGTCACAGCCCACAGTGTGCAAAAACCCAGTTGGGGACCACTTGCAGCTGAGGCTCTTTAAGAACAAAGTTGATTACTGTAGGATTCATGGGCTCGACATATTTTACAACAATGTTTTGCTGGATCCCAAGGGTACTGGGTTCTGGGCTAAATATCCGCTTTTGAGAGCGGCTATGTTGGCCCATCCCGAGTCTGAGTGGATCTGGTGGGTCGACTCAGACGCGGTTCTCACCGACATGGAGTTCAAGCTTCCGTTGGAGAAGTACAAAGACCACAACTTGGTCGTGCATGGGTGGTGGAACATGTTGTATGAGCAGAAGAGCTGGACGAGCCTCAACGCTGGGGTTTTGTTAATAAGAAACTGTCAGTGGTCACTGAACTTGCTCGATAAGTGGGCCAGCATGGGCCCACAGGGTCCCGATCCCAAAAACTGGGGGAAGATCCAAAAGTCCTTGATCAAGGACAAAGCATATCCAGGCTCTGACGATCAGTCGGCTCTGATTTATCTCTTGATAAAGGAGAAGTCGAAATGGGCGGATAAGATTTACTTAGAGAGCGAGTACAACTTGCATGGGTACTGGCTGGGGATAGTGGACGGGCTTGATGGGATAAGCAAAGGGTACCTGGAGATTGACAGGGAGGTGGATTTGCTAAGGAGGAGGCATGCAGAGAAGGTCAGCTTGTTTTATGGTCAAATGAGAGAGAAGTACATGAGAGAGAGAGGGATTTGGAGAGAGAATAAGAGGAGGCCATTTGTGACGCATTTCACAGGGTGTGAGCCTTGCAGTGGAGAGCACAATGGAATGTACACTTGGGAGGCTTGTTGGAACGGGATGCAAAAAGCTTTGAATTTTGCAGATAATCAAGTGCTTAGAAGATTTGGGTTTGTTCATCCAGACCTACTCAATTCTTCTTGGGTTTCTCCTTTGCCATTTGATTTCCCAGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_34

Protein Analysis

443

Amino Acids

50.73

Weight (kDa)

6.47

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_34 PF05637 118 - 389 9.5e-54 galactosyl transferase GMA12/MNN10 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 996
AccB1I GGYRCC 1 cut(s) 996
AccB7I CCANNNNNTGG 2 cut(s) 655, 807
AccBSI CCGCTC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 588
AccII CGCG 2 cut(s) 266, 599
AciI CCGC 7 cut(s) 201, 224, 345, 533, 545, 599, 911
AclWI GGATC 5 cut(s) 494, 507, 584, 791, 811
AcsI RAATTY 2 cut(s) 14, 1225
AcuI CTGAAG 1 cut(s) 335
AdeI CACNNNGTG 2 cut(s) 393, 1149
AfaI GTAC 7 cut(s) 511, 641, 938, 953, 998, 1085, 1184
AfiI CCNNNNNNNGG 4 cut(s) 409, 655, 807, 1163
AflIII ACRYGT 2 cut(s) 264, 678
AgsI TTSAA 2 cut(s) 622, 1225
AhdI GACNNNNNGTC 1 cut(s) 865
AjnI CCWGG 2 cut(s) 850, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 8 cut(s) 165, 261, 319, 424, 625, 699, 1056, 1220
AluI AGCT 8 cut(s) 165, 261, 319, 424, 625, 699, 1056, 1220
Alw21I GWGCWC 1 cut(s) 1173
AlwI GGATC 5 cut(s) 494, 507, 584, 791, 811
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 5 cut(s) 194, 555, 771, 781, 1124
ApaI GGGCCC 1 cut(s) 785
ApeKI GCWGC 2 cut(s) 335, 421
ApoI RAATTY 2 cut(s) 14, 1225
Asp718I GGTACC 1 cut(s) 996
AspLEI GCGC 2 cut(s) 216, 303
AspS9I GGNCC 8 cut(s) 194, 203, 412, 556, 771, 781, 782, 791
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 3 cut(s) 27, 130, 598
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 3 cut(s) 203, 412, 791
BaeGI GKGCMC 1 cut(s) 785
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 996
BanII GRGCYC 3 cut(s) 133, 471, 785
BauI CACGAG 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 1173
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 2 cut(s) 322, 433
BccI CCATC 4 cut(s) 40, 74, 567, 974
BceAI ACGGC 1 cut(s) 332
BciT130I CCWGG 2 cut(s) 852, 1001
BclI TGATCA 1 cut(s) 831
BcnI CCSGG 1 cut(s) 193
BfmI CTRYAG 1 cut(s) 454
BisI GCNGC 4 cut(s) 336, 345, 422, 546
BlsI GCNGC 4 cut(s) 337, 346, 423, 547
Bme1390I CCNGG 3 cut(s) 193, 852, 1001
Bme18I GGWCC 3 cut(s) 203, 412, 791
BmeRI GACNNNNNGTC 1 cut(s) 865
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 8 cut(s) 194, 203, 412, 556, 771, 781, 782, 791
BmrFI CCNGG 3 cut(s) 193, 852, 1001
BmrI ACTGGG 4 cut(s) 384, 398, 522, 817
BmsI GCATC 3 cut(s) 113, 1200, 1312
BmuI ACTGGG 4 cut(s) 384, 398, 522, 817
BplI GAGNNNNNCTC 2 cut(s) 351, 383
BpmI CTGGAG 1 cut(s) 1022
Bpu10I CCTNAGC 2 cut(s) 425, 1028
BpuMI CCSGG 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 348, 504
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 4 cut(s) 409, 655, 807, 1163
Bse1I ACTGG 6 cut(s) 278, 379, 404, 517, 812, 959
BseBI CCWGG 2 cut(s) 852, 1001
BseDI CCNNGG 2 cut(s) 348, 504
BseGI GGATG 3 cut(s) 559, 1215, 1267
BseLI CCNNNNNNNGG 4 cut(s) 409, 655, 807, 1163
BseMII CTCAG 5 cut(s) 24, 271, 416, 561, 606
BseNI ACTGG 6 cut(s) 278, 379, 404, 517, 812, 959
BseRI GAGGAG 3 cut(s) 125, 1048, 1135
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 2 cut(s) 322, 433
BseYI CCCAGC 2 cut(s) 716, 958
Bsh1236I CGCG 2 cut(s) 266, 599
BshFI GGCC 5 cut(s) 196, 557, 773, 783, 1126
BshNI GGYRCC 1 cut(s) 996
BsiHKAI GWGCWC 1 cut(s) 1173
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 192, 227, 339
BslFI GGGAC 3 cut(s) 305, 425, 777
BslI CCNNNNNNNGG 4 cut(s) 409, 655, 807, 1163
BsmFI GGGAC 3 cut(s) 305, 425, 777
BsnI GGCC 5 cut(s) 196, 557, 773, 783, 1126
BsoBI CYCGRG 1 cut(s) 563
Bsp120I GGGCCC 1 cut(s) 781
Bsp1286I GDGCHC 4 cut(s) 133, 471, 785, 1173
Bsp1407I TGTACA 1 cut(s) 1182
Bsp143I GATC 6 cut(s) 499, 576, 796, 816, 831, 862
BspACI CCGC 7 cut(s) 201, 224, 345, 533, 545, 599, 911
BspANI GGCC 5 cut(s) 196, 557, 773, 783, 1126
BspCNI CTCAG 5 cut(s) 23, 270, 417, 562, 605
BspFNI CGCG 2 cut(s) 266, 599
BspPI GGATC 5 cut(s) 494, 507, 584, 791, 811
BspQI GCTCTTC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 996
BsrBI CCGCTC 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 1182
BsrI ACTGG 6 cut(s) 278, 379, 404, 517, 812, 959
BssECI CCNNGG 2 cut(s) 348, 504
BssMI GATC 6 cut(s) 499, 576, 796, 816, 831, 862
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 2 cut(s) 852, 1001
Bst4CI ACNGT 3 cut(s) 391, 455, 740
Bst6I CTCTTC 3 cut(s) 29, 95, 689
BstAUI TGTACA 1 cut(s) 1182
BstC8I GCNNGC 2 cut(s) 775, 1041
BstDEI CTNAG 8 cut(s) 10, 257, 425, 570, 592, 925, 1028, 1247
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 3 cut(s) 559, 1215, 1267
BstFNI CGCG 2 cut(s) 266, 599
BstHHI GCGC 2 cut(s) 216, 303
BstKTI GATC 6 cut(s) 502, 579, 799, 819, 834, 865
BstMBI GATC 6 cut(s) 499, 576, 796, 816, 831, 862
BstMWI GCNNNNNNNGC 7 cut(s) 110, 188, 341, 344, 427, 554, 705
BstNI CCWGG 2 cut(s) 852, 1001
BstNSI RCATGY 2 cut(s) 682, 1043
BstSCI CCNGG 3 cut(s) 191, 850, 999
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 785
BstUI CGCG 2 cut(s) 266, 599
BstV1I GCAGC 2 cut(s) 322, 433
BstX2I RGATCY 3 cut(s) 499, 576, 816
BstYI RGATCY 3 cut(s) 499, 576, 816
BsuRI GGCC 5 cut(s) 196, 557, 773, 783, 1126
BtgI CCRYGG 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 3 cut(s) 559, 1215, 1267
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 5 cut(s) 285, 396, 749, 749, 1168
Cac8I GCNNGC 2 cut(s) 775, 1041
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 216, 303
Cfr13I GGNCC 8 cut(s) 194, 203, 412, 556, 771, 781, 782, 791
CseI GACGC 2 cut(s) 605, 1144
Csp6I GTAC 7 cut(s) 510, 640, 937, 952, 997, 1084, 1183
CviQI GTAC 7 cut(s) 510, 640, 937, 952, 997, 1084, 1183
DdeI CTNAG 8 cut(s) 10, 257, 425, 570, 592, 925, 1028, 1247
DpnI GATC 6 cut(s) 501, 578, 798, 818, 833, 864
DpnII GATC 6 cut(s) 499, 576, 796, 816, 831, 862
DraIII CACNNNGTG 2 cut(s) 393, 1149
DriI GACNNNNNGTC 1 cut(s) 865
Eam1104I CTCTTC 3 cut(s) 29, 95, 689
Eam1105I GACNNNNNGTC 1 cut(s) 865
EarI CTCTTC 3 cut(s) 29, 95, 689
EciI GGCGGA 1 cut(s) 926
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 3 cut(s) 133, 471, 785
Eco47I GGWCC 3 cut(s) 203, 412, 791
Eco57I CTGAAG 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 791
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 850, 999
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 3 cut(s) 133, 471, 785
ErhI CCWWGG 1 cut(s) 504
FalI AAGNNNNNCTT 4 cut(s) 908, 940, 1040, 1072
FaqI GGGAC 3 cut(s) 305, 425, 777
FauI CCCGC 1 cut(s) 231
FbaI TGATCA 1 cut(s) 831
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 4 cut(s) 336, 345, 422, 546
FokI GGATG 3 cut(s) 546, 1222, 1254
FriOI GRGCYC 3 cut(s) 133, 471, 785
Fsp4HI GCNGC 4 cut(s) 336, 345, 422, 546
GlaI GCGC 2 cut(s) 215, 302
GluI GCNGC 4 cut(s) 336, 345, 422, 546
GsaI CCCAGC 2 cut(s) 720, 962
GsuI CTGGAG 1 cut(s) 1022
HaeIII GGCC 5 cut(s) 196, 557, 773, 783, 1126
HapII CCGG 3 cut(s) 192, 227, 339
HgaI GACGC 2 cut(s) 605, 1144
HhaI GCGC 2 cut(s) 216, 303
Hin6I GCGC 2 cut(s) 214, 301
HinP1I GCGC 2 cut(s) 214, 301
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 3 cut(s) 163, 623, 1218
HinfI GANTC 4 cut(s) 293, 460, 566, 590
HpaII CCGG 3 cut(s) 192, 227, 339
HphI GGTGA 3 cut(s) 27, 130, 598
Hpy166II GTNNAC 4 cut(s) 352, 589, 970, 1185
Hpy188I TCNGA 7 cut(s) 13, 98, 220, 571, 595, 859, 876
Hpy188III TCNNGA 5 cut(s) 370, 563, 794, 886, 1271
Hpy8I GTNNAC 4 cut(s) 352, 589, 970, 1185
Hpy99I CGWCG 3 cut(s) 98, 248, 251
HpyAV CCTTC 5 cut(s) 75, 151, 277, 336, 1042
HpyCH4III ACNGT 3 cut(s) 391, 455, 740
HpyCH4IV ACGT 1 cut(s) 236
HpyCH4V TGCA 9 cut(s) 104, 396, 421, 664, 946, 1043, 1161, 1213, 1232
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 188, 341, 344, 427, 554, 705
HpyF3I CTNAG 8 cut(s) 10, 257, 425, 570, 592, 925, 1028, 1247
HpySE526I ACGT 1 cut(s) 236
HspAI GCGC 2 cut(s) 214, 301
KflI GGGWCCC 1 cut(s) 791
KpnI GGTACC 1 cut(s) 1000
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 6 cut(s) 499, 576, 796, 816, 831, 862
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 50, 324, 341
Lsp1109I GCAGC 2 cut(s) 322, 433
LweI GCATC 3 cut(s) 113, 1200, 1312
MaeII ACGT 1 cut(s) 236
MaeIII GTNAC 4 cut(s) 370, 378, 747, 1132
MalI GATC 6 cut(s) 501, 578, 798, 818, 833, 864
MbiI CCGCTC 1 cut(s) 545
MboI GATC 6 cut(s) 499, 576, 796, 816, 831, 862
MboII GAAGA 7 cut(s) 16, 82, 356, 706, 826, 1263, 1278
MflI RGATCY 3 cut(s) 499, 576, 816
MhlI GDGCHC 4 cut(s) 133, 471, 785, 1173
MluCI AATT 3 cut(s) 14, 1225, 1282
MluI ACGCGT 1 cut(s) 264
MlyI GAGTC 3 cut(s) 287, 575, 584
MmeI TCCRAC 4 cut(s) 52, 76, 612, 1181
MseI TTAA 2 cut(s) 435, 728
MslI CAYNNNNRTG 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 3 cut(s) 192, 227, 339
MspR9I CCNGG 3 cut(s) 193, 852, 1001
MvaI CCWGG 2 cut(s) 852, 1001
MvnI CGCG 2 cut(s) 266, 599
MwoI GCNNNNNNNGC 7 cut(s) 110, 188, 341, 344, 427, 554, 705
NciI CCSGG 1 cut(s) 193
NdeII GATC 6 cut(s) 499, 576, 796, 816, 831, 862
NmuCI GTSAC 4 cut(s) 370, 378, 747, 1132
NspI RCATGY 2 cut(s) 682, 1043
PaeI GCATGC 1 cut(s) 1043
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 689
PfeI GAWTC 1 cut(s) 460
PflFI GACNNNGTC 1 cut(s) 376
PflMI CCANNNNNTGG 2 cut(s) 655, 807
PkrI GCNGC 4 cut(s) 337, 346, 423, 547
PleI GAGTC 3 cut(s) 287, 574, 584
PpsI GAGTC 3 cut(s) 287, 574, 584
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 850, 999
PspFI CCCAGC 2 cut(s) 716, 958
PspGI CCWGG 2 cut(s) 850, 999
PspOMI GGGCCC 1 cut(s) 781
PspPI GGNCC 8 cut(s) 194, 203, 412, 556, 771, 781, 782, 791
PspPPI RGGWCCY 1 cut(s) 791
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 3 cut(s) 499, 576, 816
PsyI GACNNNGTC 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 7 cut(s) 511, 641, 938, 953, 998, 1085, 1184
RsaNI GTAC 7 cut(s) 510, 640, 937, 952, 997, 1084, 1183
RseI CAYNNNNRTG 1 cut(s) 611
SalI GTCGAC 1 cut(s) 587
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 2 cut(s) 435, 728
SatI GCNGC 4 cut(s) 336, 345, 422, 546
Sau3AI GATC 6 cut(s) 499, 576, 796, 816, 831, 862
Sau96I GGNCC 8 cut(s) 194, 203, 412, 556, 771, 781, 782, 791
SchI GAGTC 3 cut(s) 287, 575, 584
ScrFI CCNGG 3 cut(s) 193, 852, 1001
SduI GDGCHC 4 cut(s) 133, 471, 785, 1173
SfaNI GCATC 3 cut(s) 113, 1200, 1312
SfcI CTRYAG 1 cut(s) 454
SinI GGWCC 3 cut(s) 203, 412, 791
SmiMI CAYNNNNRTG 1 cut(s) 611
SphI GCATGC 1 cut(s) 1043
Sse9I AATT 3 cut(s) 14, 1225, 1282
SsiI CCGC 7 cut(s) 201, 224, 345, 533, 545, 599, 911
StyD4I CCNGG 3 cut(s) 191, 850, 999
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 391, 455, 740
TaiI ACGT 1 cut(s) 239
TaqI TCGA 5 cut(s) 168, 471, 588, 762, 902
TasI AATT 3 cut(s) 14, 1225, 1282
TatI WGTACW 4 cut(s) 639, 936, 1083, 1182
TauI GCSGC 2 cut(s) 347, 548
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 2 cut(s) 435, 728
Tru9I TTAA 2 cut(s) 435, 728
TscAI CASTG 5 cut(s) 285, 396, 749, 756, 1168
TseFI GTSAC 4 cut(s) 370, 378, 747, 1132
TseI GCWGC 2 cut(s) 335, 421
Tsp45I GTSAC 4 cut(s) 370, 378, 747, 1132
TspDTI ATGAA 4 cut(s) 133, 318, 452, 1256
TspGWI ACGGA 2 cut(s) 37, 618
TspRI CASTG 5 cut(s) 285, 396, 749, 756, 1168
Tth111I GACNNNGTC 1 cut(s) 376
Van91I CCANNNNNTGG 2 cut(s) 655, 807
VpaK11BI GGWCC 3 cut(s) 203, 412, 791
XapI RAATTY 2 cut(s) 14, 1225
XceI RCATGY 2 cut(s) 682, 1043
XcmI CCANNNNNNNNNTGG 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.