MD10G1201500.v1.1

glycosyltransferase 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001277
Physical Location & Seq
Reverse (-)
29969266 .. 29970726
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1201500.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGTCTTCATGTACAAGAAACGATACTTTAGTAGCTTCCATCAATGTTGAGGATCCAGACAAAGCCAAAGTACTAGCATGCACGCTCGCCCGCGCACACCTTTATACATATAGCAAAGCTACAACTCAGGCACAGAGGTACCTTGTAGCTATCTTGGAAATGGGTTCACCAAAATACCATCCACCTAGACGTTCAGTTCTCCAAAAAGTCTCTTGTTTAATTGCTCACGGGTTCCTATTTGCCGGAGGAGCAGCTCTGGCACTGTCCTTGGTTTTGGCTCTCCTGTCTGTCATAAACCCCGACACAAACTTTGACAACATCACCCGTAACAAAAGTTCCCAAGACCTTGACTCGGGATATGACCCAGCCCAGCTGAGCCTACATTCTATGATGATCGATACATCAGCTCGATTGGGGAACCTGTTTGGTGATCATTTGCTGCTACGGTTTTTCAAGAACAAAGTAGACTATTGCAGACTCCATGGTTGTGACATTTTTTACAGCAATCTGGCGTTGCATCCAAAAGTGACTGGTTCTTGGGCGAAATGCCCGACTCTTCGCGCTGCTATGTTGGCTCATCCTGAGGCTGAGTGGATCTTATGGGTTGACTCAGACGCAATCTTCACAGACATGGACTTCAAGCTCCCTCCAGAGCGATATAATGACCATAACCTAGTTGTGCATGGCTGGTCACATGAAGTATACGTGAAGCACAGCAGGACAGGTATCAATAGCGGGGTGTTCCTGATACGCAACTGCCAGTGGTCCATGGACCTCATAGAGGAATGGATCATCATGGGGCCACGGAGTCCATACTACGAACGATGGGCACAAATACAAAGTTCAGTGATCAAAGACAAGCAAATTACAAATTCAGACGATCAAACGGGTCTGATTTATCTTCTAATAGCACACAAAGAAACCTGGGCGGAGACTCACCTAGAGAATGAATACTCCCCACACGGATACTGGTTGGCAATAGTGAACGGTCTTGACAACATAACCAAAATATACGTGAAGGTGGACAGAGAGGTGGCCGTGTTGACGAGGAGACACGCGGAGAAGGTGAGCCAGTTCTATGGAGCTATGAGAGAGCACTATCTGAAGGATAAGGACTACTGGAAAGATTATCTCAGAAGGCCTTTTCTCACGCACTTCAGTGGGTGTCAACCATGCAGTGGGCAACACAGCCCCGCTTTCACTTGGGAAGCTTGTTGGAAAGGGATGCAGAGGGCTTTAAATTTCGCAGATAATCAAGTGCTTAGCAGATTTGGCTTTGTGCATCCCGACCTACTCAACTCCACATTTGTGTCTCCCCTGCAATTTAATTTCCCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_34

Protein Analysis

447

Amino Acids

50.97

Weight (kDa)

7.3

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_34 PF05637 144 - 394 2.5e-46 galactosyl transferase GMA12/MNN10 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 1178
AccI GTMKAC 2 cut(s) 465, 702
AccII CGCG 3 cut(s) 93, 561, 1058
AciI CCGC 5 cut(s) 91, 735, 929, 1058, 1194
AclWI GGATC 4 cut(s) 47, 60, 602, 797
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 2 cut(s) 871, 1240
AcuI CTGAAG 2 cut(s) 1124, 1141
AfaI GTAC 3 cut(s) 13, 72, 140
AfiI CCNNNNNNNGG 3 cut(s) 352, 781, 1178
AgsI TTSAA 2 cut(s) 454, 640
AjnI CCWGG 1 cut(s) 923
AleI CACNNNNGTG 2 cut(s) 1158, 1307
AluBI AGCT 9 cut(s) 35, 119, 149, 254, 373, 407, 643, 1085, 1211
AluI AGCT 9 cut(s) 35, 119, 149, 254, 373, 407, 643, 1085, 1211
Alw21I GWGCWC 1 cut(s) 1098
Alw26I GTCTC 4 cut(s) 214, 926, 1045, 1317
AlwI GGATC 4 cut(s) 47, 60, 602, 797
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 3 cut(s) 800, 1035, 1139
ApeKI GCWGC 3 cut(s) 251, 439, 563
ApoI RAATTY 2 cut(s) 871, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 2 cut(s) 95, 563
AspS9I GGNCC 3 cut(s) 765, 772, 800
AsuC2I CCSGG 1 cut(s) 1334
AsuHPI GGTGA 5 cut(s) 159, 313, 440, 929, 1078
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 2 cut(s) 765, 772
AxyI CCTNAGG 1 cut(s) 582
BaeGI GKGCMC 1 cut(s) 832
BaeI ACNNNNGTAYC 4 cut(s) 15, 48, 122, 155
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 1098
BbvI GCAGC 3 cut(s) 263, 426, 550
BccI CCATC 3 cut(s) 47, 186, 819
BceAI ACGGC 1 cut(s) 1022
BciT130I CCWGG 1 cut(s) 925
BciVI GTATCC 1 cut(s) 959
BclI TGATCA 2 cut(s) 430, 849
BcnI CCSGG 1 cut(s) 1334
BcoDI GTCTC 4 cut(s) 214, 926, 1045, 1317
BfaI CTAG 4 cut(s) 74, 186, 674, 941
BfuI GTATCC 1 cut(s) 959
BisI GCNGC 3 cut(s) 252, 440, 564
BlpI GCTNAGC 2 cut(s) 374, 1262
BlsI GCNGC 3 cut(s) 253, 441, 565
BmcAI AGTACT 1 cut(s) 72
Bme1390I CCNGG 2 cut(s) 925, 1334
Bme18I GGWCC 2 cut(s) 765, 772
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 3 cut(s) 765, 772, 800
BmiI GGNNCC 5 cut(s) 54, 140, 233, 419, 801
BmrFI CCNGG 2 cut(s) 925, 1334
BmsI GCATC 3 cut(s) 526, 1215, 1291
BpmI CTGGAG 1 cut(s) 633
Bpu1102I GCTNAGC 2 cut(s) 374, 1262
BpuMI CCSGG 1 cut(s) 1334
Bsa29I ATCGAT 1 cut(s) 396
BsaAI YACGTR 2 cut(s) 706, 1015
BsaJI CCNNGG 5 cut(s) 267, 481, 768, 803, 924
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 781, 1178
Bse1I ACTGG 5 cut(s) 535, 760, 974, 1072, 1124
Bse21I CCTNAGG 1 cut(s) 582
BseBI CCWGG 1 cut(s) 925
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 5 cut(s) 267, 481, 768, 803, 924
BseGI GGATG 5 cut(s) 178, 517, 577, 1230, 1282
BseLI CCNNNNNNNGG 3 cut(s) 352, 781, 1178
BseMII CTCAG 6 cut(s) 140, 365, 573, 579, 624, 1147
BseNI ACTGG 5 cut(s) 535, 760, 974, 1072, 1124
BseRI GAGGAG 2 cut(s) 261, 1063
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 3 cut(s) 263, 426, 550
BseYI CCCAGC 2 cut(s) 364, 369
Bsh1236I CGCG 3 cut(s) 93, 561, 1058
BshFI GGCC 3 cut(s) 802, 1037, 1141
BshNI GGYRCC 1 cut(s) 138
BshVI ATCGAT 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 1098
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 2 cut(s) 243, 1334
BslI CCNNNNNNNGG 3 cut(s) 352, 781, 1178
BsmAI GTCTC 4 cut(s) 214, 926, 1045, 1317
BsnI GGCC 3 cut(s) 802, 1037, 1141
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 832, 1098
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
Bsp1720I GCTNAGC 2 cut(s) 374, 1262
Bsp19I CCATGG 2 cut(s) 481, 768
BspACI CCGC 5 cut(s) 91, 735, 929, 1058, 1194
BspANI GGCC 3 cut(s) 802, 1037, 1141
BspCNI CTCAG 6 cut(s) 139, 366, 574, 580, 623, 1146
BspDI ATCGAT 1 cut(s) 396
BspFNI CGCG 3 cut(s) 93, 561, 1058
BspLI GGNNCC 5 cut(s) 54, 140, 233, 419, 801
BspPI GGATC 4 cut(s) 47, 60, 602, 797
BspT107I GGYRCC 1 cut(s) 138
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 5 cut(s) 535, 760, 974, 1072, 1124
BssECI CCNNGG 5 cut(s) 267, 481, 768, 803, 924
BssMI GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
BssNAI GTATAC 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 267, 481, 768
Bst1107I GTATAC 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 925
Bst4CI ACNGT 3 cut(s) 264, 447, 989
Bst6I CTCTTC 1 cut(s) 561
BstAUI TGTACA 1 cut(s) 11
BstBAI YACGTR 2 cut(s) 706, 1015
BstC8I GCNNGC 4 cut(s) 79, 83, 87, 91
BstDEI CTNAG 7 cut(s) 126, 374, 582, 588, 610, 1133, 1262
BstDSI CCRYGG 3 cut(s) 481, 768, 803
BstENI CCTNNNNNAGG 1 cut(s) 779
BstF5I GGATG 5 cut(s) 178, 517, 577, 1230, 1282
BstFNI CGCG 3 cut(s) 93, 561, 1058
BstHHI GCGC 2 cut(s) 95, 563
BstKTI GATC 7 cut(s) 55, 396, 433, 597, 792, 852, 883
BstMAI GTCTC 4 cut(s) 214, 926, 1045, 1317
BstMBI GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
BstMWI GCNNNNNNNGC 4 cut(s) 248, 257, 572, 1272
BstNI CCWGG 1 cut(s) 925
BstNSI RCATGY 1 cut(s) 81
BstSCI CCNGG 2 cut(s) 923, 1332
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 3 cut(s) 93, 561, 1058
BstV1I GCAGC 3 cut(s) 263, 426, 550
BstX2I RGATCY 2 cut(s) 52, 594
BstXI CCANNNNNNTGG 1 cut(s) 1079
BstYI RGATCY 2 cut(s) 52, 594
BstZ17I GTATAC 1 cut(s) 703
Bsu15I ATCGAT 1 cut(s) 396
Bsu36I CCTNAGG 1 cut(s) 582
BsuI GTATCC 1 cut(s) 959
BsuRI GGCC 3 cut(s) 802, 1037, 1141
BsuTUI ATCGAT 1 cut(s) 396
BtgI CCRYGG 3 cut(s) 481, 768, 803
BtsCI GGATG 5 cut(s) 178, 517, 577, 1230, 1282
BtsI GCAGTG 1 cut(s) 1183
BtsIMutI CAGTG 5 cut(s) 260, 767, 852, 1165, 1183
Cac8I GCNNGC 4 cut(s) 79, 83, 87, 91
CfoI GCGC 2 cut(s) 95, 563
Cfr13I GGNCC 3 cut(s) 765, 772, 800
ClaI ATCGAT 1 cut(s) 396
CseI GACGC 1 cut(s) 623
Csp6I GTAC 3 cut(s) 12, 71, 139
CviAII CATG 9 cut(s) 9, 78, 482, 631, 683, 695, 769, 796, 1173
CviQI GTAC 3 cut(s) 12, 71, 139
DdeI CTNAG 7 cut(s) 126, 374, 582, 588, 610, 1133, 1262
DpnI GATC 7 cut(s) 54, 395, 432, 596, 791, 851, 882
DpnII GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
DraI TTTAAA 1 cut(s) 1239
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 944
Eco130I CCWWGG 3 cut(s) 267, 481, 768
Eco147I AGGCCT 1 cut(s) 1141
Eco47I GGWCC 2 cut(s) 765, 772
Eco57I CTGAAG 2 cut(s) 1124, 1141
Eco81I CCTNAGG 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 352
EcoNI CCTNNNNNAGG 1 cut(s) 779
EcoRII CCWGG 1 cut(s) 923
EcoT14I CCWWGG 3 cut(s) 267, 481, 768
ErhI CCWWGG 3 cut(s) 267, 481, 768
FaeI CATG 9 cut(s) 12, 81, 485, 634, 686, 698, 772, 799, 1176
FatI CATG 9 cut(s) 8, 77, 481, 630, 682, 694, 768, 795, 1172
FauI CCCGC 3 cut(s) 98, 728, 1201
FbaI TGATCA 2 cut(s) 430, 849
FblI GTMKAC 2 cut(s) 465, 702
Fnu4HI GCNGC 3 cut(s) 252, 440, 564
FokI GGATG 5 cut(s) 165, 504, 564, 1237, 1269
Fsp4HI GCNGC 3 cut(s) 252, 440, 564
FspBI CTAG 4 cut(s) 74, 186, 674, 941
GlaI GCGC 2 cut(s) 94, 562
GluI GCNGC 3 cut(s) 252, 440, 564
GsaI CCCAGC 2 cut(s) 368, 373
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 802, 1037, 1141
HapII CCGG 2 cut(s) 243, 1334
HgaI GACGC 1 cut(s) 623
HhaI GCGC 2 cut(s) 95, 563
Hin1II CATG 9 cut(s) 12, 81, 485, 634, 686, 698, 772, 799, 1176
Hin6I GCGC 2 cut(s) 93, 561
HinP1I GCGC 2 cut(s) 93, 561
HincII GTYRAC 3 cut(s) 607, 1044, 1169
HindII GTYRAC 3 cut(s) 607, 1044, 1169
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 6 cut(s) 350, 477, 553, 608, 808, 934
HpaII CCGG 2 cut(s) 243, 1334
HphI GGTGA 5 cut(s) 159, 313, 440, 929, 1078
Hpy166II GTNNAC 8 cut(s) 167, 466, 607, 703, 985, 1024, 1044, 1169
Hpy188I TCNGA 5 cut(s) 613, 877, 894, 1104, 1136
Hpy188III TCNNGA 8 cut(s) 56, 354, 454, 581, 650, 745, 992, 1286
Hpy8I GTNNAC 8 cut(s) 167, 466, 607, 703, 985, 1024, 1044, 1169
HpyAV CCTTC 4 cut(s) 1012, 1057, 1099, 1131
HpyCH4III ACNGT 3 cut(s) 264, 447, 989
HpyCH4IV ACGT 3 cut(s) 190, 705, 1014
HpyCH4V TGCA 8 cut(s) 81, 474, 517, 682, 1176, 1228, 1282, 1321
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 257, 572, 1272
HpyF3I CTNAG 7 cut(s) 126, 374, 582, 588, 610, 1133, 1262
HpySE526I ACGT 3 cut(s) 190, 705, 1014
Hsp92II CATG 9 cut(s) 12, 81, 485, 634, 686, 698, 772, 799, 1176
HspAI GCGC 2 cut(s) 93, 561
KpnI GGTACC 1 cut(s) 142
Ksp22I TGATCA 2 cut(s) 430, 849
Kzo9I GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
LmnI GCTCC 3 cut(s) 248, 648, 1082
Lsp1109I GCAGC 3 cut(s) 263, 426, 550
LweI GCATC 3 cut(s) 526, 1215, 1291
MaeI CTAG 4 cut(s) 74, 186, 674, 941
MaeII ACGT 3 cut(s) 190, 705, 1014
MaeIII GTNAC 4 cut(s) 326, 488, 526, 690
MalI GATC 7 cut(s) 54, 395, 432, 596, 791, 851, 882
MboI GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
MboII GAAGA 3 cut(s) 548, 613, 893
MflI RGATCY 2 cut(s) 52, 594
MhlI GDGCHC 2 cut(s) 832, 1098
MluCI AATT 6 cut(s) 219, 864, 871, 1240, 1322, 1327
MlyI GAGTC 6 cut(s) 344, 471, 547, 602, 817, 928
MmeI TCCRAC 1 cut(s) 1196
MseI TTAA 3 cut(s) 218, 1238, 1326
MslI CAYNNNNRTG 4 cut(s) 486, 629, 1158, 1307
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 2 cut(s) 243, 1334
MspR9I CCNGG 2 cut(s) 925, 1334
MvaI CCWGG 1 cut(s) 925
MvnI CGCG 3 cut(s) 93, 561, 1058
MwoI GCNNNNNNNGC 4 cut(s) 248, 257, 572, 1272
NciI CCSGG 1 cut(s) 1334
NcoI CCATGG 2 cut(s) 481, 768
NdeII GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
NlaIII CATG 9 cut(s) 12, 81, 485, 634, 686, 698, 772, 799, 1176
NlaIV GGNNCC 5 cut(s) 54, 140, 233, 419, 801
NmuCI GTSAC 3 cut(s) 488, 526, 690
NspI RCATGY 1 cut(s) 81
OliI CACNNNNGTG 2 cut(s) 1158, 1307
PaeI GCATGC 1 cut(s) 81
PceI AGGCCT 1 cut(s) 1141
PflMI CCANNNNNTGG 1 cut(s) 1178
PkrI GCNGC 3 cut(s) 253, 441, 565
PleI GAGTC 6 cut(s) 344, 471, 547, 602, 816, 928
PpsI GAGTC 6 cut(s) 344, 471, 547, 602, 816, 928
Ppu21I YACGTR 2 cut(s) 706, 1015
Psp6I CCWGG 1 cut(s) 923
PspFI CCCAGC 2 cut(s) 364, 369
PspGI CCWGG 1 cut(s) 923
PspN4I GGNNCC 5 cut(s) 54, 140, 233, 419, 801
PspPI GGNCC 3 cut(s) 765, 772, 800
PsrI GAACNNNNNNTAC 2 cut(s) 319, 351
PsuI RGATCY 2 cut(s) 52, 594
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 3 cut(s) 13, 72, 140
RsaNI GTAC 3 cut(s) 12, 71, 139
RseI CAYNNNNRTG 4 cut(s) 486, 629, 1158, 1307
SaqAI TTAA 3 cut(s) 218, 1238, 1326
SatI GCNGC 3 cut(s) 252, 440, 564
Sau3AI GATC 7 cut(s) 52, 393, 430, 594, 789, 849, 880
Sau96I GGNCC 3 cut(s) 765, 772, 800
ScaI AGTACT 1 cut(s) 72
SchI GAGTC 6 cut(s) 344, 471, 547, 602, 817, 928
ScrFI CCNGG 2 cut(s) 925, 1334
SduI GDGCHC 2 cut(s) 832, 1098
SfaNI GCATC 3 cut(s) 526, 1215, 1291
SinI GGWCC 2 cut(s) 765, 772
SmiMI CAYNNNNRTG 4 cut(s) 486, 629, 1158, 1307
SphI GCATGC 1 cut(s) 81
Sse9I AATT 6 cut(s) 219, 864, 871, 1240, 1322, 1327
SseBI AGGCCT 1 cut(s) 1141
SsiI CCGC 5 cut(s) 91, 735, 929, 1058, 1194
SspMI CTAG 4 cut(s) 74, 186, 674, 941
StuI AGGCCT 1 cut(s) 1141
StyD4I CCNGG 2 cut(s) 923, 1332
StyI CCWWGG 3 cut(s) 267, 481, 768
TaaI ACNGT 3 cut(s) 264, 447, 989
TaiI ACGT 3 cut(s) 193, 708, 1017
TaqI TCGA 2 cut(s) 396, 409
TasI AATT 6 cut(s) 219, 864, 871, 1240, 1322, 1327
TatI WGTACW 2 cut(s) 11, 70
Tru1I TTAA 3 cut(s) 218, 1238, 1326
Tru9I TTAA 3 cut(s) 218, 1238, 1326
TscAI CASTG 5 cut(s) 267, 767, 852, 1165, 1183
TseFI GTSAC 3 cut(s) 488, 526, 690
TseI GCWGC 3 cut(s) 251, 439, 563
Tsp45I GTSAC 3 cut(s) 488, 526, 690
TspDTI ATGAA 2 cut(s) 711, 963
TspGWI ACGGA 2 cut(s) 820, 978
TspRI CASTG 5 cut(s) 267, 767, 852, 1165, 1183
Van91I CCANNNNNTGG 1 cut(s) 1178
VpaK11BI GGWCC 2 cut(s) 765, 772
XagI CCTNNNNNAGG 1 cut(s) 779
XapI RAATTY 2 cut(s) 871, 1240
XceI RCATGY 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XmiI GTMKAC 2 cut(s) 465, 702
XspI CTAG 4 cut(s) 74, 186, 674, 941
ZrmI AGTACT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.