pycom09g14400

glycosyltransferase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001277
Physical Location & Seq
Forward (+)
13912859 .. 13913477
619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14400.1

Sequence Viewer

Length: 561 bp
ATGGTGCTTAACAAAACCTCCACCCAACTCTTCCACCTCCGCCTTCAGTGCGACGCCTTCAGTGTCACAACCCTTACCTCACTCGTAGCTTCTGCGATATCCCTAAACAGAACAACCAGAGGCGTTTTCTCCATCTCCAAACACTTCTCTGTTTTCTTTCTCTTCTACACTCTACACCATCACTGTCCCTCATCGTCATTCCTCATCACCGTCCCCCACGGCCTCTCTCTCTTTAATTTTTCTGCCACTGATCCGGAAGCTCATTCTGTCCCACCTAGAGGTGAGTTCCTCTGGTGGATGGATTCCGACGCCATGTTCACCGACATGGCATTCGAGGTCCCCTGGGAGCAATACAAGAATCACAATTTCGTTATGCATGAATGGAACGAGATGGTGTACGATGACAAGAACTGGATCGGGTTGAACACCGGGTCGTTCCTACTCAAAAATTACCAGTGGTCGCTCGACATTCTAGACGTCTGGGCACTGATAGGTCCAAAGGGGAAGATCCGAGATGAGGTACAGGTTAGGGGGATTATGGAGGTGTTTGGTTACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_34

Protein Analysis

187

Amino Acids

21.59

Weight (kDa)

6.11

Isoelectric Point (pI)

22.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_34 PF05637 92 - 162 1.2e-17 galactosyl transferase GMA12/MNN10 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 253
AciI CCGC 1 cut(s) 40
AclWI GGATC 3 cut(s) 245, 422, 502
AcuI CTGAAG 2 cut(s) 29, 43
AcyI GRCGYC 3 cut(s) 54, 309, 477
AfaI GTAC 2 cut(s) 398, 522
AfiI CCNNNNNNNGG 2 cut(s) 278, 517
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 1 cut(s) 341
AluBI AGCT 2 cut(s) 89, 260
AluI AGCT 2 cut(s) 89, 260
AlwI GGATC 3 cut(s) 245, 422, 502
Aor13HI TCCGGA 1 cut(s) 253
AoxI GGCC 1 cut(s) 220
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AspS9I GGNCC 2 cut(s) 337, 494
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 3 cut(s) 199, 293, 310
AvaII GGWCC 2 cut(s) 337, 494
BaeGI GKGCMC 1 cut(s) 487
BccI CCATC 4 cut(s) 140, 186, 292, 385
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 343
BcnI CCSGG 1 cut(s) 430
BfaI CTAG 2 cut(s) 276, 473
Bme1390I CCNGG 2 cut(s) 343, 430
Bme18I GGWCC 2 cut(s) 337, 494
BmgT120I GGNCC 2 cut(s) 337, 494
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 2 cut(s) 343, 430
BpuMI CCSGG 1 cut(s) 430
BsaHI GRCGYC 3 cut(s) 54, 309, 477
BsaJI CCNNGG 3 cut(s) 217, 341, 342
BsaWI WCCGGW 1 cut(s) 253
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 517
Bse1I ACTGG 2 cut(s) 416, 454
BseAI TCCGGA 1 cut(s) 253
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 217, 341, 342
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 278, 517
BseNI ACTGG 2 cut(s) 416, 454
BseSI GKGCMC 1 cut(s) 487
BshFI GGCC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 254, 429
BslFI GGGAC 4 cut(s) 171, 197, 254, 323
BslI CCNNNNNNNGG 2 cut(s) 278, 517
BsmFI GGGAC 4 cut(s) 171, 197, 254, 323
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 487
Bsp13I TCCGGA 1 cut(s) 253
Bsp143I GATC 3 cut(s) 250, 414, 507
BspACI CCGC 1 cut(s) 40
BspANI GGCC 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 3 cut(s) 245, 422, 502
BsrI ACTGG 2 cut(s) 416, 454
BssECI CCNNGG 3 cut(s) 217, 341, 342
BssMI GATC 3 cut(s) 250, 414, 507
BssNI GRCGYC 3 cut(s) 54, 309, 477
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 185, 211
Bst6I CTCTTC 2 cut(s) 35, 167
BstACI GRCGYC 3 cut(s) 54, 309, 477
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 1 cut(s) 303
BstKTI GATC 3 cut(s) 253, 417, 510
BstMBI GATC 3 cut(s) 250, 414, 507
BstMWI GCNNNNNNNGC 1 cut(s) 48
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 2 cut(s) 341, 428
BstSLI GKGCMC 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 507
BstYI RGATCY 1 cut(s) 507
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 303
BtsIMutI CAGTG 6 cut(s) 53, 67, 181, 246, 461, 485
Cfr13I GGNCC 2 cut(s) 337, 494
CseI GACGC 2 cut(s) 62, 317
Csp6I GTAC 2 cut(s) 397, 521
CviAII CATG 3 cut(s) 313, 325, 377
CviJI RGCY 3 cut(s) 89, 222, 260
CviKI_1 RGCY 3 cut(s) 89, 222, 260
CviQI GTAC 2 cut(s) 397, 521
DpnI GATC 3 cut(s) 252, 416, 509
DpnII GATC 3 cut(s) 250, 414, 507
Eam1104I CTCTTC 2 cut(s) 35, 167
EarI CTCTTC 2 cut(s) 35, 167
EciI GGCGGA 1 cut(s) 29
Eco32I GATATC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 337, 494
Eco57I CTGAAG 2 cut(s) 29, 43
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 341
EcoRV GATATC 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 378
FaeI CATG 3 cut(s) 316, 328, 380
FaiI YATR 5 cut(s) 314, 326, 374, 378, 539
FaqI GGGAC 4 cut(s) 171, 197, 254, 323
FatI CATG 3 cut(s) 312, 324, 376
FokI GGATG 1 cut(s) 310
FspBI CTAG 2 cut(s) 276, 473
HaeIII GGCC 1 cut(s) 222
HapII CCGG 2 cut(s) 254, 429
HgaI GACGC 2 cut(s) 62, 317
Hin1I GRCGYC 3 cut(s) 54, 309, 477
Hin1II CATG 3 cut(s) 316, 328, 380
HinfI GANTC 2 cut(s) 302, 358
HpaII CCGG 2 cut(s) 254, 429
HphI GGTGA 3 cut(s) 199, 293, 310
Hpy166II GTNNAC 2 cut(s) 318, 397
Hpy188I TCNGA 2 cut(s) 307, 512
Hpy188III TCNNGA 2 cut(s) 254, 473
Hpy8I GTNNAC 2 cut(s) 318, 397
Hpy99I CGWCG 2 cut(s) 56, 311
HpyAV CCTTC 2 cut(s) 53, 67
HpyCH4III ACNGT 2 cut(s) 185, 211
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 48
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 3 cut(s) 54, 309, 477
Hsp92II CATG 3 cut(s) 316, 328, 380
Kpn2I TCCGGA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 250, 414, 507
LmnI GCTCC 1 cut(s) 346
MaeI CTAG 2 cut(s) 276, 473
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 2 cut(s) 64, 551
MalI GATC 3 cut(s) 252, 416, 509
MboI GATC 3 cut(s) 250, 414, 507
MboII GAAGA 3 cut(s) 22, 154, 517
MflI RGATCY 1 cut(s) 507
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 3 cut(s) 235, 364, 448
MmeI TCCRAC 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 378
MroI TCCGGA 1 cut(s) 253
MseI TTAA 2 cut(s) 9, 234
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 2 cut(s) 254, 429
MspR9I CCNGG 2 cut(s) 343, 430
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 430
NdeII GATC 3 cut(s) 250, 414, 507
NlaIII CATG 3 cut(s) 316, 328, 380
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 64
NsiI ATGCAT 1 cut(s) 378
PasI CCCWGGG 1 cut(s) 342
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 302, 358
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 337, 494
PspPPI RGGWCCY 1 cut(s) 337
PsuI RGATCY 1 cut(s) 507
RsaI GTAC 2 cut(s) 398, 522
RsaNI GTAC 2 cut(s) 397, 521
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 9, 234
Sau3AI GATC 3 cut(s) 250, 414, 507
Sau96I GGNCC 2 cut(s) 337, 494
ScrFI CCNGG 2 cut(s) 343, 430
SduI GDGCHC 1 cut(s) 487
SinI GGWCC 2 cut(s) 337, 494
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 3 cut(s) 235, 364, 448
SsiI CCGC 1 cut(s) 40
SspMI CTAG 2 cut(s) 276, 473
StyD4I CCNGG 2 cut(s) 341, 428
TaaI ACNGT 2 cut(s) 185, 211
TaiI ACGT 1 cut(s) 480
TaqI TCGA 2 cut(s) 333, 465
TasI AATT 3 cut(s) 235, 364, 448
TfiI GAWTC 2 cut(s) 302, 358
Tru1I TTAA 2 cut(s) 9, 234
Tru9I TTAA 2 cut(s) 9, 234
TscAI CASTG 6 cut(s) 53, 67, 188, 253, 461, 492
TseFI GTSAC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 6 cut(s) 53, 67, 188, 253, 461, 492
VpaK11BI GGWCC 2 cut(s) 337, 494
XbaI TCTAGA 1 cut(s) 472
XspI CTAG 2 cut(s) 276, 473
ZraI GACGTC 1 cut(s) 478
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.