pycom10g17260

glycosyltransferase 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001277
Physical Location & Seq
Reverse (-)
20481053 .. 20482471
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17260.1

Sequence Viewer

Length: 1419 bp
ATGCTTCTTCCTACTTACACTGCTCCAAGCGTACAAAACAGATTTAGGCAAGCTACCACTTCCCCACTACTTTCACTACTCTCTTCAAAAATGGGTTCCTCAGAATTCTCTCTCTTCCAACAATCACCAAAACGATCCGCTTTCCACAAAGCCCCTTCCCTAATAGCAGATGGCTTTCTCTTCGTCGGCGGTGCATCATTGGCTCTCTCTGTGGTCTGGGCTTTCCTCACCTTCATCAACCCCACCACCATAAGCTTCGACAGCATCCTCGCCTGGAACGCGGGTCCATGCGGACCCGACTTGAGCTCCGACCCGCCAGAGCCCACGTTCTACGACGACGAAAATCTCAGCTACGCGTTTGGGGAGCCAGTGAAGGATTGGGACTCGAAGCGCAGAGAGTGGCTGAAGCTGCACCCTTCATTCGCCGCCGGAGCCGAGGCGGATAACAGGGTTCTTCTGGTAACTGGGTCCCAGCCCACCGTATGCAAAAACCCAGTTGGTGACCACTTACAGCTGAGGTTCTTCAAGAACAAAGTTGATTACTGTAGGATTCATGGGCATGACATATTTTACAACAGTGTTTTGTTGGATCCCAAGGGTACTGGATTTTGGGCCAAATACCCGCTATTGAGAGCGGCTATGTTGGCTCATCCCGAGTCCGAGTGGATCTGGTGGGTCGACTCAGACGCGGTTCTCACCGACATGGAGTTCAAGCTTCCGTTGGAGAGGTACAAAGACCATAACTTGGTCGTGCATGGGTGGTGGAACATGGTGTATGAGCAGAAGAGCTGGACGAGCCTCAACGCTGGGGTTTTGTTGATAAGAAACTGTCAGTGGTCATTGAGCTTGCTGGATAAGTGGGCCAGCATGGGTCCACAGGGTCCGAATCCTGAAAAATGGGGGAAGATCCAAAAGTCCCTGATCAAGGACAAGGCGTATCCGGGCTCTGACGATCAGTCGGCTCTGGTTTATCTCTTGATCAAGGAGAAGTCGAGATGGGCGGCTAAGATTTACTTAGAGAGCGAGTACAACTTGCATGGATACTGGCTGGGGATAGTGGACGGGCTTGATGGGATAAGCAAAGGGTACATGGAGATTGACAGGGAGGTGGATTTGCTGAGGAGGAGGCATGCTGAGAAGGTCAGCTTGTTTTATGGTCAAATGAGAGAGAAGTACATGAGAGAGAGAGGGATTTGGAGAGAGAATAAGAGGAGGCCATTTGTGACACATTTCACAGGGTGTGAGCCGTGCAGTGGAGAGCACAATGGAATGTACACTTGGGAGGCTTGTTGGAATGGGATGCAAAAAGCTTTGAATTTTGCAGATAACCAAGTGCTTAGGAGATTTGGGTTTGTTCATCCAGACCTACTCAATTCATCTTTGGTTTCTCCTTTGCCATTTGATTTCCCAGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_34

Protein Analysis

473

Amino Acids

53.98

Weight (kDa)

6.9

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_34 PF05637 148 - 419 5.9e-55 galactosyl transferase GMA12/MNN10 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 745
AccBSI CCGCTC 1 cut(s) 635
AccI GTMKAC 1 cut(s) 678
AccII CGCG 3 cut(s) 281, 356, 689
AclWI GGATC 5 cut(s) 129, 584, 597, 674, 901
AcsI RAATTY 2 cut(s) 104, 1315
AcuI CTGAAG 1 cut(s) 425
AdeI CACNNNGTG 1 cut(s) 1239
AfaI GTAC 7 cut(s) 33, 601, 731, 1028, 1088, 1175, 1274
AfiI CCNNNNNNNGG 3 cut(s) 745, 925, 1253
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 4 cut(s) 87, 526, 712, 1315
AhdI GACNNNNNGTC 1 cut(s) 955
AjnI CCWGG 1 cut(s) 272
AjuI GAANNNNNNNTTGG 2 cut(s) 1261, 1293
Alw21I GWGCWC 2 cut(s) 308, 1263
AlwI GGATC 5 cut(s) 129, 584, 597, 674, 901
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 3 cut(s) 612, 861, 1214
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 2 cut(s) 104, 1315
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 7 cut(s) 284, 293, 468, 612, 861, 872, 881
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 4 cut(s) 117, 220, 512, 688
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 5 cut(s) 284, 293, 468, 872, 881
BamHI GGATCC 1 cut(s) 589
BanII GRGCYC 3 cut(s) 308, 324, 947
Bbv12I GWGCWC 2 cut(s) 308, 1263
BbvCI CCTCAGC 2 cut(s) 515, 1118
BbvI GCAGC 1 cut(s) 396
BccI CCATC 3 cut(s) 164, 990, 1064
BceAI ACGGC 1 cut(s) 1231
BciT130I CCWGG 1 cut(s) 274
BciVI GTATCC 2 cut(s) 948, 1034
BclI TGATCA 2 cut(s) 921, 978
BcnI CCSGG 1 cut(s) 942
BfmI CTRYAG 1 cut(s) 544
BfuI GTATCC 2 cut(s) 948, 1034
BisI GCNGC 4 cut(s) 410, 426, 636, 1002
BlsI GCNGC 4 cut(s) 411, 427, 637, 1003
Bme1390I CCNGG 2 cut(s) 274, 942
Bme18I GGWCC 5 cut(s) 284, 293, 468, 872, 881
BmeRI GACNNNNNGTC 1 cut(s) 955
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 7 cut(s) 284, 293, 468, 612, 861, 872, 881
BmrFI CCNGG 2 cut(s) 274, 942
BmrI ACTGGG 2 cut(s) 474, 488
BmsI GCATC 4 cut(s) 203, 273, 1290, 1402
BmuI ACTGGG 2 cut(s) 474, 488
Bpu10I CCTNAGC 3 cut(s) 515, 1118, 1337
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 435, 594
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 3 cut(s) 745, 925, 1253
Bse1I ACTGG 5 cut(s) 368, 469, 494, 607, 1049
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 435, 594
BseGI GGATG 4 cut(s) 264, 649, 1305, 1357
BseLI CCNNNNNNNGG 3 cut(s) 745, 925, 1253
BseMII CTCAG 6 cut(s) 114, 361, 506, 696, 1109, 1125
BseNI ACTGG 5 cut(s) 368, 469, 494, 607, 1049
BseRI GAGGAG 3 cut(s) 1135, 1138, 1225
BseXI GCAGC 1 cut(s) 396
BseYI CCCAGC 3 cut(s) 471, 806, 1048
BsgI GTGCAG 2 cut(s) 395, 1270
Bsh1236I CGCG 3 cut(s) 281, 356, 689
BshFI GGCC 3 cut(s) 614, 863, 1216
BsiHKAI GWGCWC 2 cut(s) 308, 1263
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 2 cut(s) 429, 941
BslFI GGGAC 3 cut(s) 395, 454, 901
BslI CCNNNNNNNGG 3 cut(s) 745, 925, 1253
BsmFI GGGAC 3 cut(s) 395, 454, 901
BsnI GGCC 3 cut(s) 614, 863, 1216
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 4 cut(s) 308, 324, 947, 1263
Bsp1407I TGTACA 1 cut(s) 1272
Bsp143I GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
BspANI GGCC 3 cut(s) 614, 863, 1216
BspCNI CTCAG 6 cut(s) 113, 360, 507, 695, 1110, 1126
BspFNI CGCG 3 cut(s) 281, 356, 689
BspPI GGATC 5 cut(s) 129, 584, 597, 674, 901
BspQI GCTCTTC 1 cut(s) 779
BsrBI CCGCTC 1 cut(s) 635
BsrGI TGTACA 1 cut(s) 1272
BsrI ACTGG 5 cut(s) 368, 469, 494, 607, 1049
BssECI CCNNGG 2 cut(s) 435, 594
BssMI GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 4 cut(s) 481, 545, 578, 830
Bst6I CTCTTC 4 cut(s) 88, 119, 185, 779
BstAUI TGTACA 1 cut(s) 1272
BstC8I GCNNGC 4 cut(s) 51, 848, 865, 1131
BstDEI CTNAG 9 cut(s) 100, 347, 515, 682, 1005, 1015, 1118, 1134, 1337
BstEII GGTNACC 1 cut(s) 500
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 4 cut(s) 264, 649, 1305, 1357
BstFNI CGCG 3 cut(s) 281, 356, 689
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 7 cut(s) 137, 592, 669, 909, 924, 955, 981
BstMBI GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
BstMWI GCNNNNNNNGC 7 cut(s) 200, 261, 278, 409, 431, 644, 795
BstNI CCWGG 1 cut(s) 274
BstNSI RCATGY 1 cut(s) 1133
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 2 cut(s) 272, 940
BstSFI CTRYAG 1 cut(s) 544
BstUI CGCG 3 cut(s) 281, 356, 689
BstV1I GCAGC 1 cut(s) 396
BstX2I RGATCY 3 cut(s) 589, 666, 906
BstYI RGATCY 3 cut(s) 589, 666, 906
BsuI GTATCC 2 cut(s) 948, 1034
BsuRI GGCC 3 cut(s) 614, 863, 1216
BtsCI GGATG 4 cut(s) 264, 649, 1305, 1357
BtsI GCAGTG 2 cut(s) 18, 1258
BtsIMutI CAGTG 5 cut(s) 18, 375, 583, 839, 1258
Cac8I GCNNGC 4 cut(s) 51, 848, 865, 1131
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 7 cut(s) 284, 293, 468, 612, 861, 872, 881
CseI GACGC 1 cut(s) 695
Csp6I GTAC 7 cut(s) 32, 600, 730, 1027, 1087, 1174, 1273
CviQI GTAC 7 cut(s) 32, 600, 730, 1027, 1087, 1174, 1273
DdeI CTNAG 9 cut(s) 100, 347, 515, 682, 1005, 1015, 1118, 1134, 1337
DpnI GATC 7 cut(s) 136, 591, 668, 908, 923, 954, 980
DpnII GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
DraIII CACNNNGTG 1 cut(s) 1239
DriI GACNNNNNGTC 1 cut(s) 955
Eam1104I CTCTTC 4 cut(s) 88, 119, 185, 779
Eam1105I GACNNNNNGTC 1 cut(s) 955
EarI CTCTTC 4 cut(s) 88, 119, 185, 779
EciI GGCGGA 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 594
Eco24I GRGCYC 3 cut(s) 308, 324, 947
Eco47I GGWCC 5 cut(s) 284, 293, 468, 872, 881
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 306
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoO109I RGGNCCY 1 cut(s) 468
EcoO65I GGTNACC 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 272
EcoT14I CCWWGG 1 cut(s) 594
EcoT38I GRGCYC 3 cut(s) 308, 324, 947
ErhI CCWWGG 1 cut(s) 594
FalI AAGNNNNNCTT 4 cut(s) 998, 1030, 1130, 1162
FaqI GGGAC 3 cut(s) 395, 454, 901
FauI CCCGC 3 cut(s) 274, 321, 630
FbaI TGATCA 2 cut(s) 921, 978
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 4 cut(s) 410, 426, 636, 1002
FokI GGATG 4 cut(s) 251, 636, 1312, 1344
FriOI GRGCYC 3 cut(s) 308, 324, 947
Fsp4HI GCNGC 4 cut(s) 410, 426, 636, 1002
GlaI GCGC 1 cut(s) 392
GluI GCNGC 4 cut(s) 410, 426, 636, 1002
GsaI CCCAGC 3 cut(s) 475, 810, 1052
HaeIII GGCC 3 cut(s) 614, 863, 1216
HapII CCGG 2 cut(s) 429, 941
HgaI GACGC 1 cut(s) 695
HhaI GCGC 1 cut(s) 393
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 3 cut(s) 253, 713, 1308
HinfI GANTC 5 cut(s) 383, 550, 656, 680, 886
HpaII CCGG 2 cut(s) 429, 941
HphI GGTGA 4 cut(s) 117, 220, 512, 688
Hpy166II GTNNAC 4 cut(s) 679, 875, 1060, 1275
Hpy188I TCNGA 6 cut(s) 103, 310, 661, 685, 885, 949
Hpy188III TCNNGA 6 cut(s) 526, 653, 890, 976, 993, 1361
Hpy8I GTNNAC 4 cut(s) 679, 875, 1060, 1275
Hpy99I CGWCG 3 cut(s) 188, 338, 341
HpyAV CCTTC 5 cut(s) 165, 241, 367, 426, 1132
HpyCH4III ACNGT 4 cut(s) 481, 545, 578, 830
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 8 cut(s) 194, 412, 486, 754, 1036, 1251, 1303, 1322
HpyF10VI GCNNNNNNNGC 7 cut(s) 200, 261, 278, 409, 431, 644, 795
HpyF3I CTNAG 9 cut(s) 100, 347, 515, 682, 1005, 1015, 1118, 1134, 1337
HpySE526I ACGT 1 cut(s) 326
HspAI GCGC 1 cut(s) 391
KflI GGGWCCC 1 cut(s) 468
Ksp22I TGATCA 2 cut(s) 921, 978
Kzo9I GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 4 cut(s) 28, 311, 364, 431
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 4 cut(s) 203, 273, 1290, 1402
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 3 cut(s) 460, 500, 1222
MalI GATC 7 cut(s) 136, 591, 668, 908, 923, 954, 980
MbiI CCGCTC 1 cut(s) 635
MboI GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
MboII GAAGA 7 cut(s) 75, 106, 172, 446, 514, 796, 916
MflI RGATCY 3 cut(s) 589, 666, 906
MhlI GDGCHC 4 cut(s) 308, 324, 947, 1263
MluCI AATT 3 cut(s) 104, 1315, 1372
MluI ACGCGT 1 cut(s) 354
MlyI GAGTC 3 cut(s) 377, 665, 674
MmeI TCCRAC 5 cut(s) 142, 333, 567, 702, 1271
MslI CAYNNNNRTG 2 cut(s) 558, 701
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 2 cut(s) 429, 941
MspR9I CCNGG 2 cut(s) 274, 942
MvaI CCWGG 1 cut(s) 274
MvnI CGCG 3 cut(s) 281, 356, 689
MwoI GCNNNNNNNGC 7 cut(s) 200, 261, 278, 409, 431, 644, 795
NciI CCSGG 1 cut(s) 942
NdeII GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 2 cut(s) 500, 1222
NspI RCATGY 1 cut(s) 1133
PaeI GCATGC 1 cut(s) 1133
PciSI GCTCTTC 1 cut(s) 779
PfeI GAWTC 2 cut(s) 550, 886
PflMI CCANNNNNTGG 1 cut(s) 745
PkrI GCNGC 4 cut(s) 411, 427, 637, 1003
PleI GAGTC 3 cut(s) 377, 664, 674
PpsI GAGTC 3 cut(s) 377, 664, 674
PpuMI RGGWCCY 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 272
PspEI GGTNACC 1 cut(s) 500
PspFI CCCAGC 3 cut(s) 471, 806, 1048
PspGI CCWGG 1 cut(s) 272
PspPI GGNCC 7 cut(s) 284, 293, 468, 612, 861, 872, 881
PspPPI RGGWCCY 1 cut(s) 468
PsuI RGATCY 3 cut(s) 589, 666, 906
PvuII CAGCTG 1 cut(s) 514
RsaI GTAC 7 cut(s) 33, 601, 731, 1028, 1088, 1175, 1274
RsaNI GTAC 7 cut(s) 32, 600, 730, 1027, 1087, 1174, 1273
RseI CAYNNNNRTG 2 cut(s) 558, 701
SacI GAGCTC 1 cut(s) 308
SalI GTCGAC 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 779
SatI GCNGC 4 cut(s) 410, 426, 636, 1002
Sau3AI GATC 7 cut(s) 134, 589, 666, 906, 921, 952, 978
Sau96I GGNCC 7 cut(s) 284, 293, 468, 612, 861, 872, 881
SchI GAGTC 3 cut(s) 377, 665, 674
ScrFI CCNGG 2 cut(s) 274, 942
SduI GDGCHC 4 cut(s) 308, 324, 947, 1263
SfaNI GCATC 4 cut(s) 203, 273, 1290, 1402
SfcI CTRYAG 1 cut(s) 544
SinI GGWCC 5 cut(s) 284, 293, 468, 872, 881
SmiMI CAYNNNNRTG 2 cut(s) 558, 701
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SphI GCATGC 1 cut(s) 1133
Sse9I AATT 3 cut(s) 104, 1315, 1372
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 272, 940
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 4 cut(s) 481, 545, 578, 830
TaiI ACGT 1 cut(s) 329
TaqI TCGA 4 cut(s) 258, 386, 678, 992
TasI AATT 3 cut(s) 104, 1315, 1372
TatI WGTACW 3 cut(s) 1026, 1173, 1272
TauI GCSGC 3 cut(s) 428, 638, 1004
TfiI GAWTC 2 cut(s) 550, 886
TscAI CASTG 5 cut(s) 25, 375, 583, 839, 1258
TseFI GTSAC 2 cut(s) 500, 1222
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 2 cut(s) 500, 1222
TspDTI ATGAA 5 cut(s) 223, 408, 542, 1346, 1365
TspGWI ACGGA 1 cut(s) 708
TspRI CASTG 5 cut(s) 25, 375, 583, 839, 1258
Van91I CCANNNNNTGG 1 cut(s) 745
VpaK11BI GGWCC 5 cut(s) 284, 293, 468, 872, 881
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 2 cut(s) 104, 1315
XceI RCATGY 1 cut(s) 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.