MD05G1250900.v1.1

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003266
Physical Location & Seq
Forward (+)
38296771 .. 38299991
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1250900.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGAAGACGCGATATCATCAACTTTTGTTTCAGGCTGGGCATGGCTCCCGAGCGACCTTCTACATTTGATTGTAGAAAAGTTGATACCAACCTCTGACTATATCCGATTTGGTGCAGTTTGTAAGCATTGGCAATCAGTCGCTTTGCTTCAGAAGCAGCGGCGCCTCAAATCACATCAACAACAGCTTCCAATGCTGATTATTCCCAGCAAAGACAACATCAATGATAGGCGTGGCTTGTATAGTGTCATGCATGGTAAGAATTGTGGCTTTGAGTTGTATGTGCCTTACAACAACAGGTGCTGCGGTTCTTCCCACGGTTGGTTGGCTTGTGTGAATGACAATTTCGGGGTAAATCTCTTCAACCCTTTTATCAAACGTACCATTCTCCTTCCACCAGTAACCAAAGTCCCCCAGGACATACGAAAACATGCTTATAGAAGAGATCACTACATTAAGAAGGTTGTATTGTCTGCAGACCCTTCTTTGTGTCCAAATGATTACGAGGTTGTGGCACTTTTCGATTGTAATGGAACCAGAATAGCTCATATCAAGTCAGGGGACAATGCTTGGACTCACATAGATCAAACAATCAGATATTTTTATGGTCTGCATCAAATAATAGGATTCGATGATGTACTATACTATAGAGGCCAGTTTCTTGCTGCTAGTCGGGAGGGTGGAGTTTTTTCGATTAACATTAGCAATGACTCAACTCATAAGCCTCATGTAAGTATAGTTTTACCAATAGTTCCAAGTATTGATGACAAAGCATACCTTGTGGAATCGTCCCAGGGAGATCTTTTGCTGGTCAGGAAGTTCCAAAGTTTGAATTACAGCGAGTGTTTCATTGAAAACTTTAGCTTCAAGGTTTTCAAGCTGTTCAGTGCTTCCGGGGGTAGAACTGGACCCGAATGGATCGAGGAGATTGAGAGTATTGGAGATGATGCTTTTTTCATGGGTGACAACCAGTCCATATGTGTCTCTGCTTCAGACTTTCCAGGATGTCAGCCGAATTCCATATACTTCTGCATTGATGATCGGGTGGGTGTGGCTTATGAGGAAGCTCAAGGATCTCTTGACATGGCAGTTTTCAATTTGGAAAACAGAAGATTCGGAAGACGTTACTACTGCTTAAGCCGTTCGCAGAAGAACATGCCGCCATCTATTTGGATTTTGCCCACCATGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

398

Amino Acids

45.18

Weight (kDa)

6.85

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 96 - 366 5.3e-52 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 160, 306, 1160
AclWI GGATC 2 cut(s) 926, 1081
AcsI RAATTY 1 cut(s) 1015
AcuI CTGAAG 2 cut(s) 134, 975
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 2 cut(s) 382, 639
AfiI CCNNNNNNNGG 1 cut(s) 321
AflII CTTAAG 1 cut(s) 1135
AgsI TTSAA 6 cut(s) 364, 832, 854, 868, 877, 1096
AhdI GACNNNNNGTC 1 cut(s) 970
AjnI CCWGG 3 cut(s) 414, 792, 1000
AleI CACNNNNGTG 1 cut(s) 1187
AluBI AGCT 5 cut(s) 187, 545, 864, 880, 1067
AluI AGCT 5 cut(s) 187, 545, 864, 880, 1067
Alw26I GTCTC 1 cut(s) 988
AlwI GGATC 2 cut(s) 926, 1081
AlwNI CAGNNNCTG 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 3 cut(s) 157, 303, 665
ApoI RAATTY 1 cut(s) 1015
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 908
AsuC2I CCSGG 1 cut(s) 895
AsuHPI GGTGA 1 cut(s) 974
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 908
BanI GGYRCC 1 cut(s) 162
BbsI GAAGAC 2 cut(s) 12, 1126
BbvI GCAGC 3 cut(s) 169, 290, 652
BccI CCATC 1 cut(s) 1171
BceAI ACGGC 1 cut(s) 1125
BciT130I CCWGG 3 cut(s) 416, 794, 1002
BcnI CCSGG 1 cut(s) 895
BcoDI GTCTC 1 cut(s) 988
BfaI CTAG 1 cut(s) 669
BfmI CTRYAG 2 cut(s) 474, 646
BfoI RGCGCY 1 cut(s) 166
BfrI CTTAAG 1 cut(s) 1135
BglII AGATCT 1 cut(s) 799
BisI GCNGC 5 cut(s) 158, 161, 304, 666, 1160
BlsI GCNGC 5 cut(s) 159, 162, 305, 667, 1161
Bme1390I CCNGG 4 cut(s) 416, 794, 895, 1002
Bme18I GGWCC 1 cut(s) 908
BmeRI GACNNNNNGTC 1 cut(s) 970
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 4 cut(s) 47, 164, 535, 910
BmrFI CCNGG 4 cut(s) 416, 794, 895, 1002
BmsI GCATC 2 cut(s) 622, 937
BpiI GAAGAC 2 cut(s) 12, 1126
BpuEI CTTGAG 1 cut(s) 1053
BpuMI CCSGG 1 cut(s) 895
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 6 cut(s) 316, 414, 792, 793, 894, 1185
Bsc4I CCNNNNNNNGG 1 cut(s) 321
Bse1I ACTGG 4 cut(s) 398, 655, 910, 970
Bse3DI GCAATG 1 cut(s) 712
BseBI CCWGG 3 cut(s) 416, 794, 1002
BseDI CCNNGG 6 cut(s) 316, 414, 792, 793, 894, 1185
BseGI GGATG 1 cut(s) 1010
BseLI CCNNNNNNNGG 1 cut(s) 321
BseMI GCAATG 1 cut(s) 712
BseNI ACTGG 4 cut(s) 398, 655, 910, 970
BseRI GAGGAG 1 cut(s) 938
BseXI GCAGC 3 cut(s) 169, 290, 652
BseYI CCCAGC 2 cut(s) 36, 206
BsgI GTGCAG 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 654
BshNI GGYRCC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 1 cut(s) 894
BslFI GGGAC 3 cut(s) 395, 575, 775
BslI CCNNNNNNNGG 1 cut(s) 321
BsmAI GTCTC 1 cut(s) 988
BsmFI GGGAC 3 cut(s) 395, 575, 775
BsnI GGCC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
Bsp19I CCATGG 1 cut(s) 1185
BspACI CCGC 3 cut(s) 160, 306, 1160
BspANI GGCC 1 cut(s) 654
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 4 cut(s) 47, 164, 535, 910
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 2 cut(s) 926, 1081
BspT107I GGYRCC 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 1135
BsrDI GCAATG 1 cut(s) 712
BsrI ACTGG 4 cut(s) 398, 655, 910, 970
BssECI CCNNGG 6 cut(s) 316, 414, 792, 793, 894, 1185
BssMI GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
BssNI GRCGYC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 1185
Bst2UI CCWGG 3 cut(s) 416, 794, 1002
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 2 cut(s) 365, 436
BstACI GRCGYC 1 cut(s) 163
BstAFI CTTAAG 1 cut(s) 1135
BstDSI CCRYGG 2 cut(s) 316, 1185
BstF5I GGATG 1 cut(s) 1010
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 6 cut(s) 448, 586, 802, 921, 1042, 1076
BstMAI GTCTC 1 cut(s) 988
BstMBI GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
BstMWI GCNNNNNNNGC 2 cut(s) 154, 193
BstNI CCWGG 3 cut(s) 416, 794, 1002
BstNSI RCATGY 2 cut(s) 434, 1159
BstSCI CCNGG 4 cut(s) 414, 792, 893, 1000
BstSFI CTRYAG 2 cut(s) 474, 646
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 169, 290, 652
BstV2I GAAGAC 2 cut(s) 12, 1126
BstX2I RGATCY 2 cut(s) 799, 1073
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 2 cut(s) 799, 1073
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 2 cut(s) 316, 1185
BtsCI GGATG 1 cut(s) 1010
BtsIMutI CAGTG 1 cut(s) 892
CaiI CAGNNNCTG 1 cut(s) 303
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 908
CseI GACGC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 381, 638
CviAII CATG 9 cut(s) 42, 250, 254, 431, 728, 958, 1084, 1156, 1186
CviQI GTAC 2 cut(s) 381, 638
DinI GGCGCC 1 cut(s) 164
DpnI GATC 6 cut(s) 447, 585, 801, 920, 1041, 1075
DpnII GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
DriI GACNNNNNGTC 1 cut(s) 970
Eam1104I CTCTTC 2 cut(s) 365, 436
Eam1105I GACNNNNNGTC 1 cut(s) 970
EarI CTCTTC 2 cut(s) 365, 436
Eco130I CCWWGG 1 cut(s) 1185
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 908
Eco57I CTGAAG 2 cut(s) 134, 975
Eco88I CYCGRG 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 3 cut(s) 414, 792, 1000
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 1185
EcoT22I ATGCAT 1 cut(s) 255
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 1185
FaeI CATG 9 cut(s) 45, 253, 257, 434, 731, 961, 1087, 1159, 1189
FalI AAGNNNNNCTT 4 cut(s) 762, 794, 1062, 1094
FaqI GGGAC 3 cut(s) 395, 575, 775
FatI CATG 9 cut(s) 41, 249, 253, 430, 727, 957, 1083, 1155, 1185
FauNDI CATATG 1 cut(s) 977
Fnu4HI GCNGC 5 cut(s) 158, 161, 304, 666, 1160
FokI GGATG 1 cut(s) 1017
Fsp4HI GCNGC 5 cut(s) 158, 161, 304, 666, 1160
FspBI CTAG 1 cut(s) 669
GlaI GCGC 1 cut(s) 164
GluI GCNGC 5 cut(s) 158, 161, 304, 666, 1160
GsaI CCCAGC 2 cut(s) 40, 210
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 654
HapII CCGG 1 cut(s) 894
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 9 cut(s) 45, 253, 257, 434, 731, 961, 1087, 1159, 1189
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HinfI GANTC 5 cut(s) 574, 627, 710, 785, 1113
HpaII CCGG 1 cut(s) 894
HphI GGTGA 1 cut(s) 974
Hpy188I TCNGA 6 cut(s) 97, 107, 153, 596, 994, 1118
Hpy188III TCNNGA 4 cut(s) 49, 674, 814, 1079
HpyAV CCTTC 4 cut(s) 68, 401, 454, 492
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 2 cut(s) 379, 1123
HpyCH4V TGCA 5 cut(s) 116, 253, 476, 613, 1032
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 193
HpySE526I ACGT 2 cut(s) 379, 1123
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 9 cut(s) 45, 253, 257, 434, 731, 961, 1087, 1159, 1189
HspAI GCGC 1 cut(s) 163
KasI GGCGCC 1 cut(s) 162
Kzo9I GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 3 cut(s) 169, 290, 652
LweI GCATC 2 cut(s) 622, 937
MaeI CTAG 1 cut(s) 669
MaeII ACGT 2 cut(s) 379, 1123
MaeIII GTNAC 3 cut(s) 400, 962, 1124
MalI GATC 6 cut(s) 447, 585, 801, 920, 1041, 1075
MboI GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
MboII GAAGA 7 cut(s) 17, 303, 352, 453, 1122, 1131, 1162
MflI RGATCY 2 cut(s) 799, 1073
MluCI AATT 5 cut(s) 262, 343, 832, 1015, 1096
Mly113I GGCGCC 1 cut(s) 163
MlyI GAGTC 2 cut(s) 568, 704
MnlI CCTC 8 cut(s) 103, 176, 499, 644, 670, 735, 916, 1054
Mph1103I ATGCAT 1 cut(s) 255
MseI TTAA 3 cut(s) 456, 696, 1136
MslI CAYNNNNRTG 1 cut(s) 1187
MspA1I CMGCKG 1 cut(s) 160
MspCI CTTAAG 1 cut(s) 1135
MspI CCGG 1 cut(s) 894
MspR9I CCNGG 4 cut(s) 416, 794, 895, 1002
MvaI CCWGG 3 cut(s) 416, 794, 1002
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 154, 193
NarI GGCGCC 1 cut(s) 163
NciI CCSGG 1 cut(s) 895
NcoI CCATGG 1 cut(s) 1185
NdeI CATATG 1 cut(s) 977
NdeII GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
NlaIII CATG 9 cut(s) 45, 253, 257, 434, 731, 961, 1087, 1159, 1189
NlaIV GGNNCC 4 cut(s) 47, 164, 535, 910
NmuCI GTSAC 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 255
NspI RCATGY 2 cut(s) 434, 1159
OliI CACNNNNGTG 1 cut(s) 1187
PasI CCCWGGG 1 cut(s) 793
PfeI GAWTC 3 cut(s) 627, 785, 1113
PfoI TCCNGGA 1 cut(s) 1000
PkrI GCNGC 5 cut(s) 159, 162, 305, 667, 1161
PleI GAGTC 2 cut(s) 568, 704
PluTI GGCGCC 1 cut(s) 166
PpsI GAGTC 2 cut(s) 568, 704
Psp6I CCWGG 3 cut(s) 414, 792, 1000
PspFI CCCAGC 2 cut(s) 36, 206
PspGI CCWGG 3 cut(s) 414, 792, 1000
PspN4I GGNNCC 4 cut(s) 47, 164, 535, 910
PspPI GGNCC 1 cut(s) 908
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 303
PsuI RGATCY 2 cut(s) 799, 1073
RsaI GTAC 2 cut(s) 382, 639
RsaNI GTAC 2 cut(s) 381, 638
RseI CAYNNNNRTG 1 cut(s) 1187
SaqAI TTAA 3 cut(s) 456, 696, 1136
SatI GCNGC 5 cut(s) 158, 161, 304, 666, 1160
Sau3AI GATC 6 cut(s) 445, 583, 799, 918, 1039, 1073
Sau96I GGNCC 1 cut(s) 908
SchI GAGTC 2 cut(s) 568, 704
ScrFI CCNGG 4 cut(s) 416, 794, 895, 1002
SfaNI GCATC 2 cut(s) 622, 937
SfcI CTRYAG 2 cut(s) 474, 646
SfoI GGCGCC 1 cut(s) 164
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 2 cut(s) 1068, 1135
SmoI CTYRAG 2 cut(s) 1068, 1135
Sse9I AATT 5 cut(s) 262, 343, 832, 1015, 1096
SsiI CCGC 3 cut(s) 160, 306, 1160
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 4 cut(s) 414, 792, 893, 1000
StyI CCWWGG 1 cut(s) 1185
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 2 cut(s) 382, 1126
TaqI TCGA 4 cut(s) 522, 630, 692, 921
TasI AATT 5 cut(s) 262, 343, 832, 1015, 1096
TatI WGTACW 1 cut(s) 637
TauI GCSGC 2 cut(s) 163, 1162
TfiI GAWTC 3 cut(s) 627, 785, 1113
Tru1I TTAA 3 cut(s) 456, 696, 1136
Tru9I TTAA 3 cut(s) 456, 696, 1136
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 3 cut(s) 157, 303, 665
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 2 cut(s) 838, 946
TspRI CASTG 1 cut(s) 892
Vha464I CTTAAG 1 cut(s) 1135
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 1 cut(s) 1015
XceI RCATGY 2 cut(s) 434, 1159
XspI CTAG 1 cut(s) 669
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.