Rh5DG252100

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003266
Physical Location & Seq
Forward (+)
29090611 .. 29094865
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG252100.1

Sequence Viewer

Length: 609 bp
ATGAAGTATTGTGAATCTTCCCATGGTTGGTTGGCTTATGCACAAGGCAATTTGGTAGCAATAACCCTCTTAAACCCTTTCACCGTACGCACCATCACCCTTCCACCGGTACCAGCAACCGTTCCACCAGTACGAGCAACAGGTTCCTACTACATCAAGAAAGTTGCACTGTCTGCTGACCCTTCTTCGTTCCCTGATGATTATCAAGTTCTAGTAATTTATAACGAGCGTGTTTATAATGCTGACACCAGGATAGCCCATTTTAAGTCGGGGGAAGATACTTGGACTCTCGTAGATAGTGGGACGACTATCAGATTCACCCATGCAGTTTGCTACAAAGGTCAATTTCTAGTTTGTGGTGGAACAAATGTTGATTTTTTGGTTTTTGAGGTAGATGTCTGCAGAAAAAGAATTACTCAACTCATGTATGGTGAACAATTTGTGCGCCGACATGAGGTTATGAATACATACCTTGTGGAATCGTCAAAGGGAGATCTATTGCTGGTAACGAGTGTTGGAATCAATACTGAATGGGGATTGTTGATGGCAAGTATATCTCTGAGGATACCTATAAAGAAACTCTTCTACAGGTTCTTGCATTTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

202

Amino Acids

22.92

Weight (kDa)

8.63

Isoelectric Point (pI)

20.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 2 - 171 4.3e-25 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 222, 237
Acc65I GGTACC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 109
AfaI GTAC 3 cut(s) 87, 111, 132
AfiI CCNNNNNNNGG 3 cut(s) 27, 106, 454
AgeI ACCGGT 1 cut(s) 106
AjnI CCWGG 1 cut(s) 248
AsiGI ACCGGT 1 cut(s) 106
Asp700I GAANNNNTTC 1 cut(s) 581
Asp718I GGTACC 1 cut(s) 109
AspLEI GCGC 1 cut(s) 447
AsuHPI GGTGA 4 cut(s) 73, 88, 310, 443
BanI GGYRCC 1 cut(s) 109
BccI CCATC 2 cut(s) 101, 538
BciT130I CCWGG 1 cut(s) 250
BciVI GTATCC 1 cut(s) 558
BfaI CTAG 3 cut(s) 212, 350, 607
BfmI CTRYAG 2 cut(s) 400, 586
BfuI GTATCC 1 cut(s) 558
BglII AGATCT 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 250
BmiI GGNNCC 2 cut(s) 111, 145
BmrFI CCNGG 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 22
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 106, 454
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 27, 106, 454
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 109
BshTI ACCGGT 1 cut(s) 106
BsiSI CCGG 1 cut(s) 107
BsiWI CGTACG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 3 cut(s) 27, 106, 454
BsmFI GGGAC 1 cut(s) 316
Bsp143I GATC 1 cut(s) 493
Bsp19I CCATGG 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 111, 145
BspMAI CTGCAG 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 3 cut(s) 85, 121, 171
Bst6I CTCTTC 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 1 cut(s) 496
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 248
BstSFI CTRYAG 2 cut(s) 400, 586
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuI GTATCC 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 167
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 106
Csp6I GTAC 3 cut(s) 86, 110, 131
CspAI ACCGGT 1 cut(s) 106
CviAII CATG 4 cut(s) 23, 323, 424, 452
CviJI RGCY 2 cut(s) 35, 257
CviKI_1 RGCY 2 cut(s) 35, 257
CviQI GTAC 3 cut(s) 86, 110, 131
DdeI CTNAG 1 cut(s) 560
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 4 cut(s) 26, 326, 427, 455
FalI AAGNNNNNCTT 2 cut(s) 566, 598
FaqI GGGAC 1 cut(s) 316
FatI CATG 4 cut(s) 22, 322, 423, 451
FspBI CTAG 3 cut(s) 212, 350, 607
GlaI GCGC 1 cut(s) 446
HapII CCGG 1 cut(s) 107
HhaI GCGC 1 cut(s) 447
Hin1II CATG 4 cut(s) 26, 326, 427, 455
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HinfI GANTC 5 cut(s) 14, 286, 315, 479, 519
HpaII CCGG 1 cut(s) 107
HphI GGTGA 4 cut(s) 73, 88, 310, 443
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 2 cut(s) 314, 561
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 2 cut(s) 110, 192
HpyCH4III ACNGT 3 cut(s) 85, 121, 171
HpyCH4V TGCA 5 cut(s) 41, 167, 326, 402, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 1 cut(s) 560
Hsp92II CATG 4 cut(s) 26, 326, 427, 455
HspAI GCGC 1 cut(s) 445
KpnI GGTACC 1 cut(s) 113
Kzo9I GATC 1 cut(s) 493
LpnPI CCDG 9 cut(s) 120, 126, 126, 141, 207, 235, 262, 488, 574
MaeI CTAG 3 cut(s) 212, 350, 607
MaeIII GTNAC 1 cut(s) 505
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 4 cut(s) 9, 177, 287, 574
MflI RGATCY 1 cut(s) 493
MluCI AATT 5 cut(s) 49, 216, 344, 411, 437
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 4 cut(s) 77, 382, 448, 555
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 2 cut(s) 71, 264
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 250
MvaI CCWGG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 22
NdeII GATC 1 cut(s) 493
NlaIII CATG 4 cut(s) 26, 326, 427, 455
NlaIV GGNNCC 2 cut(s) 111, 145
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 4 cut(s) 14, 315, 479, 519
Pfl23II CGTACG 1 cut(s) 85
PinAI ACCGGT 1 cut(s) 106
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PsiI TTATAA 2 cut(s) 222, 237
Psp6I CCWGG 1 cut(s) 248
PspGI CCWGG 1 cut(s) 248
PspLI CGTACG 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 111, 145
PstI CTGCAG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 3 cut(s) 87, 111, 132
RsaNI GTAC 3 cut(s) 86, 110, 131
SaqAI TTAA 2 cut(s) 71, 264
Sau3AI GATC 1 cut(s) 493
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 250
SetI ASST 7 cut(s) 145, 343, 393, 459, 474, 571, 593
SfcI CTRYAG 2 cut(s) 400, 586
Sse9I AATT 5 cut(s) 49, 216, 344, 411, 437
SspMI CTAG 3 cut(s) 212, 350, 607
StyD4I CCNGG 1 cut(s) 248
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 3 cut(s) 85, 121, 171
TasI AATT 5 cut(s) 49, 216, 344, 411, 437
TfiI GAWTC 4 cut(s) 14, 315, 479, 519
Tru1I TTAA 2 cut(s) 71, 264
Tru9I TTAA 2 cut(s) 71, 264
TscAI CASTG 1 cut(s) 174
TspDTI ATGAA 2 cut(s) 17, 476
TspRI CASTG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 3 cut(s) 212, 350, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.