Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003266

Physical Location & Seq

Genomic Coordinates Forward (+)

32816540 .. 32817757

Gene Structure 1218 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1231500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGAAGAAGCGTCGACCTCAGTCGCAGGCTGGGCATGGCTTCCGGACGACATTCTGGGTTTGATACTAGAAAAGTTGATACCCATCTCTGACTATATCCGGTTTAGTGCAGTTTGCAAGCATTGGAAATCAGTTGCGTTACATCACAAGCAGCATCGCCTCGTCAAATCACGTCACAACCAGCTTCCAATGCTGATGGTTCCGACTAAGGACAGCAGCAACAAAAGGCGTTCCCTGTATAGCGTCACACGAGGTAAGACTTACGGCTTTGAGTTGCATGTGCCCTATAGTAAGAGGTGCTGCGGTTCTTCCCACGGTTGGTTGGCCTGTGTCGATGACAATTTGGTAGTAACCCTCTTAAACCCTTTTACCGGGCGTACCATTAGCCTTCCTCCCGTCCCCGTATCCGCGTCTAGAGCAAGGGCTGCTTTTAGATGTGATTACTACATTAACAAGGTTGTATTATCTGCTAACCCTTCATTGTTTCCAAATGATTATGAAGCTGTGGTCATCTATGATGGTTACGGAAAACGAATAGCACATATTAAGTCAGGGGATGAAGCTTGGACTTGCATAGATCAAGTGATCGGATTCGATGATGTGATTTATTACAAGGGTCAGTTTCTTGCTGTTAGTTTGGATGGCAGTGTTTTTTCGATTAATGTTAGTAGTGACCAAACTGCTAAACCCCATGTAAGCGTTGTTGTACCGATGTCTCCTGGTACTGATAACAAAACATGCCTAGCCGAATCGTCCCGGGGAGATCTATTGCTGATTAGGAAGTTCCGAAGATTGAATTATTGTAAGCGCTTTATGAAGTCTTTGAGCTTCAAGTTTTTCAAGTTTGAAAAGCTGGAGTTTGAACAAGTAAATGGTTATGATCGTTTTACGGAGACTCTGAGCTTCAAGGTTTTTAAGCTGTTCTCTTCTTATGAGGATAGACCCCAATGGGTTGAGGTTGAGAGTATCGGAAACGATGCTTTGTTTTTGGGTACAAATCAATCGATGTGTGTCTCTGCCTCGGACTTTCAAGGGTGTCGGCCAAATTCCATATACTTCACCGATGATTGTGTTGATGTTGCTTGTCATAAACCTAAAGGGCCTCATGACATGGGTGTCTTCGACATAGAAAACAAAAGCATGGGAAGGCATTACTGCTTGGATCGTTCACAGAAGCACATGCCGCCAGCAATTTGGATTTTGCCCACCATGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF295 F-box F-box-like

Protein Analysis

406

Amino Acids

45.94

Weight (kDa)

8.67

Isoelectric Point (pI)

44.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	12 - 49	3.6e-07	F-box domain
F-box-like	PF12937	14 - 48	7.7e-07	F-box-like
Beta-prop_KIB1-4	PF03478	79 - 375	2.3e-60	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003266)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g12381
malus_domestica	MD05G1250900.v1.1 MD10G1231500.v1.1
prunus_persica	Prupe.6G183700_v2.0.a1 Prupe.6G184200_v2.0.a1 Prupe.6G184600_v2.0.a1 Prupe.8G064600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0020191 RchiOBHm_Chr5g0020201
rosa_laevigata	RLG00000032508 RLG00000032510 RLG00000032511 RLG00000032585 RLG00000033606
rosa_multiflora	Rmu_sc0002087.1_g000011 Rmu_sc0002087.1_g000012 Rmu_ssc0000244.1_g000031
rosa_roxburghii	Rroxscaffold_1G00057970 Rroxscaffold_1G00058000 Rroxscaffold_1G00058010
rosa_rugosa	Rorug05G0057600 Rorug05G0057700 Rorug05G0057800
rosa_samantha	Rh5AG148000 Rh5AG148100 Rh5BG147600 Rh5BG147700 Rh5BG147800 Rh5CG159000 Rh5CG159100 Rh5CG274700 Rh5DG146800 Rh5DG146900 Rh5DG153600 Rh5DG252100
rosa_wichuraiana	Rw5G013090 Rw5G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1115
AccI	GTMKAC	1 cut(s)	14
AccII	CGCG	1 cut(s)	410
AccIII	TCCGGA	1 cut(s)	43
AciI	CCGC	3 cut(s)	303, 408, 1184
AclWI	GGATC	1 cut(s)	1170
AcoI	YGGCCR	1 cut(s)	1040
AcsI	RAATTY	1 cut(s)	1045
AfaI	GTAC	4 cut(s)	379, 708, 724, 994
AfeI	AGCGCT	1 cut(s)	809
AfiI	CCNNNNNNNGG	2 cut(s)	318, 371
AgsI	TTSAA	7 cut(s)	796, 832, 841, 848, 863, 907, 1031
AjiI	CACGTC	1 cut(s)	173
AjnI	CCWGG	1 cut(s)	718
AleI	CACNNNNGTG	1 cut(s)	1211
AluBI	AGCT	7 cut(s)	184, 503, 563, 828, 853, 903, 919
AluI	AGCT	7 cut(s)	184, 503, 563, 828, 853, 903, 919
Alw26I	GTCTC	3 cut(s)	720, 887, 1018
AlwI	GGATC	1 cut(s)	1170
Ama87I	CYCGRG	1 cut(s)	756
Aor13HI	TCCGGA	1 cut(s)	43
Aor51HI	AGCGCT	1 cut(s)	809
AoxI	GGCC	3 cut(s)	324, 1040, 1100
ApeKI	GCWGC	4 cut(s)	151, 216, 300, 425
ApoI	RAATTY	1 cut(s)	1045
AseI	ATTAAT	1 cut(s)	660
AspLEI	GCGC	1 cut(s)	810
AspS9I	GGNCC	1 cut(s)	1100
AsuC2I	CCSGG	3 cut(s)	373, 757, 758
AsuHPI	GGTGA	1 cut(s)	1051
AvaI	CYCGRG	1 cut(s)	756
BaeGI	GKGCMC	1 cut(s)	285
BauI	CACGAG	1 cut(s)	249
BbsI	GAAGAC	1 cut(s)	1111
BbvI	GCAGC	4 cut(s)	163, 228, 287, 412
BccI	CCATC	4 cut(s)	92, 190, 512, 635
BceAI	ACGGC	1 cut(s)	280
BciT130I	CCWGG	1 cut(s)	720
BciVI	GTATCC	1 cut(s)	415
BcnI	CCSGG	3 cut(s)	373, 757, 758
BcoDI	GTCTC	3 cut(s)	720, 887, 1018
BfaI	CTAG	3 cut(s)	68, 414, 743
BfmI	CTRYAG	1 cut(s)	286
BfoI	RGCGCY	1 cut(s)	811
BfuI	GTATCC	1 cut(s)	415
BglII	AGATCT	1 cut(s)	763
BisI	GCNGC	5 cut(s)	152, 217, 301, 426, 1184
BlsI	GCNGC	5 cut(s)	153, 218, 302, 427, 1185
Bme1390I	CCNGG	4 cut(s)	373, 720, 757, 758
BmeT110I	CYCGRG	1 cut(s)	756
BmgBI	CACGTC	1 cut(s)	173
BmgT120I	GGNCC	1 cut(s)	1100
BmiI	GGNNCC	1 cut(s)	201
BmrFI	CCNGG	4 cut(s)	373, 720, 757, 758
BmsI	GCATC	2 cut(s)	163, 967
BoxI	GACNNNNGTC	1 cut(s)	20
BpiI	GAAGAC	1 cut(s)	1111
BpmI	CTGGAG	1 cut(s)	875
BpuMI	CCSGG	3 cut(s)	373, 757, 758
Bsa29I	ATCGAT	1 cut(s)	1004
BsaBI	GATNNNNATC	1 cut(s)	83
BsaJI	CCNNGG	5 cut(s)	313, 756, 757, 1020, 1209
BsaWI	WCCGGW	2 cut(s)	43, 99
Bsc4I	CCNNNNNNNGG	2 cut(s)	318, 371
Bse8I	GATNNNNATC	1 cut(s)	83
BseAI	TCCGGA	1 cut(s)	43
BseBI	CCWGG	1 cut(s)	720
BseCI	ATCGAT	1 cut(s)	1004
BseDI	CCNNGG	5 cut(s)	313, 756, 757, 1020, 1209
BseGI	GGATG	2 cut(s)	562, 646
BseJI	GATNNNNATC	1 cut(s)	83
BseLI	CCNNNNNNNGG	2 cut(s)	318, 371
BseMII	CTCAG	2 cut(s)	33, 890
BseSI	GKGCMC	1 cut(s)	285
BseXI	GCAGC	4 cut(s)	163, 228, 287, 412
BseYI	CCCAGC	1 cut(s)	30
BsgI	GTGCAG	1 cut(s)	129
Bsh1236I	CGCG	1 cut(s)	410
BshFI	GGCC	3 cut(s)	326, 1042, 1102
BshVI	ATCGAT	1 cut(s)	1004
BsiHKCI	CYCGRG	1 cut(s)	756
BsiSI	CCGG	4 cut(s)	44, 100, 372, 757
BslFI	GGGAC	2 cut(s)	383, 739
BslI	CCNNNNNNNGG	2 cut(s)	318, 371
BsmAI	GTCTC	3 cut(s)	720, 887, 1018
BsmFI	GGGAC	2 cut(s)	383, 739
BsnI	GGCC	3 cut(s)	326, 1042, 1102
BsoBI	CYCGRG	1 cut(s)	756
Bsp1286I	GDGCHC	1 cut(s)	285
Bsp13I	TCCGGA	1 cut(s)	43
Bsp143I	GATC	5 cut(s)	577, 585, 763, 880, 1162
Bsp19I	CCATGG	1 cut(s)	1209
BspACI	CCGC	3 cut(s)	303, 408, 1184
BspANI	GGCC	3 cut(s)	326, 1042, 1102
BspCNI	CTCAG	2 cut(s)	32, 891
BspDI	ATCGAT	1 cut(s)	1004
BspEI	TCCGGA	1 cut(s)	43
BspFNI	CGCG	1 cut(s)	410
BspHI	TCATGA	1 cut(s)	1105
BspLI	GGNNCC	1 cut(s)	201
BspPI	GGATC	1 cut(s)	1170
BssECI	CCNNGG	5 cut(s)	313, 756, 757, 1020, 1209
BssMI	GATC	5 cut(s)	577, 585, 763, 880, 1162
BssSI	CACGAG	1 cut(s)	249
BssT1I	CCWWGG	1 cut(s)	1209
Bst2BI	CACGAG	1 cut(s)	249
Bst2UI	CCWGG	1 cut(s)	720
Bst4CI	ACNGT	1 cut(s)	317
Bst6I	CTCTTC	1 cut(s)	931
BstAPI	GCANNNNNTGC	1 cut(s)	425
BstC8I	GCNNGC	3 cut(s)	28, 119, 1188
BstDEI	CTNAG	3 cut(s)	19, 207, 899
BstDSI	CCRYGG	2 cut(s)	313, 1209
BstF5I	GGATG	2 cut(s)	562, 646
BstFNI	CGCG	1 cut(s)	410
BstH2I	RGCGCY	1 cut(s)	811
BstHHI	GCGC	1 cut(s)	810
BstKTI	GATC	5 cut(s)	580, 588, 766, 883, 1165
BstMAI	GTCTC	3 cut(s)	720, 887, 1018
BstMBI	GATC	5 cut(s)	577, 585, 763, 880, 1162
BstMWI	GCNNNNNNNGC	5 cut(s)	32, 190, 416, 425, 1183
BstNI	CCWGG	1 cut(s)	720
BstNSI	RCATGY	3 cut(s)	281, 741, 1183
BstPAI	GACNNNNGTC	1 cut(s)	20
BstSCI	CCNGG	4 cut(s)	371, 718, 755, 756
BstSFI	CTRYAG	1 cut(s)	286
BstSLI	GKGCMC	1 cut(s)	285
BstUI	CGCG	1 cut(s)	410
BstV1I	GCAGC	4 cut(s)	163, 228, 287, 412
BstV2I	GAAGAC	1 cut(s)	1111
BstX2I	RGATCY	1 cut(s)	763
BstXI	CCANNNNNNTGG	1 cut(s)	1194
BstYI	RGATCY	1 cut(s)	763
Bsu15I	ATCGAT	1 cut(s)	1004
BsuI	GTATCC	1 cut(s)	415
BsuRI	GGCC	3 cut(s)	326, 1042, 1102
BsuTUI	ATCGAT	1 cut(s)	1004
BtgI	CCRYGG	2 cut(s)	313, 1209
BtgZI	GCGATG	1 cut(s)	140
BtrI	CACGTC	1 cut(s)	173
BtsCI	GGATG	2 cut(s)	562, 646
BtsI	GCAGTG	1 cut(s)	652
BtsIMutI	CAGTG	1 cut(s)	652
Cac8I	GCNNGC	3 cut(s)	28, 119, 1188
CciI	TCATGA	1 cut(s)	1105
CfoI	GCGC	1 cut(s)	810
Cfr13I	GGNCC	1 cut(s)	1100
Cfr9I	CCCGGG	1 cut(s)	756
ClaI	ATCGAT	1 cut(s)	1004
CseI	GACGC	2 cut(s)	232, 399
Csp6I	GTAC	4 cut(s)	378, 707, 723, 993
CviAII	CATG	9 cut(s)	36, 278, 692, 738, 1106, 1111, 1141, 1180, 1210
CviQI	GTAC	4 cut(s)	378, 707, 723, 993
DdeI	CTNAG	3 cut(s)	19, 207, 899
DpnI	GATC	5 cut(s)	579, 587, 765, 882, 1164
DpnII	GATC	5 cut(s)	577, 585, 763, 880, 1162
DrdI	GACNNNNNNGTC	1 cut(s)	1115
DseDI	GACNNNNNNGTC	1 cut(s)	1115
EaeI	YGGCCR	1 cut(s)	1040
Eam1104I	CTCTTC	1 cut(s)	931
EarI	CTCTTC	1 cut(s)	931
Eco130I	CCWWGG	1 cut(s)	1209
Eco47III	AGCGCT	1 cut(s)	809
Eco88I	CYCGRG	1 cut(s)	756
EcoO109I	RGGNCCY	1 cut(s)	1100
EcoRII	CCWGG	1 cut(s)	718
EcoT14I	CCWWGG	1 cut(s)	1209
ErhI	CCWWGG	1 cut(s)	1209
FaeI	CATG	9 cut(s)	39, 281, 695, 741, 1109, 1114, 1144, 1183, 1213
FalI	AAGNNNNNCTT	2 cut(s)	412, 444
FaqI	GGGAC	2 cut(s)	383, 739
FatI	CATG	9 cut(s)	35, 277, 691, 737, 1105, 1110, 1140, 1179, 1209
FblI	GTMKAC	1 cut(s)	14
Fnu4HI	GCNGC	5 cut(s)	152, 217, 301, 426, 1184
FokI	GGATG	2 cut(s)	569, 653
Fsp4HI	GCNGC	5 cut(s)	152, 217, 301, 426, 1184
FspBI	CTAG	3 cut(s)	68, 414, 743
GlaI	GCGC	1 cut(s)	809
GluI	GCNGC	5 cut(s)	152, 217, 301, 426, 1184
GsaI	CCCAGC	1 cut(s)	34
GsuI	CTGGAG	1 cut(s)	875
HaeII	RGCGCY	1 cut(s)	811
HaeIII	GGCC	3 cut(s)	326, 1042, 1102
HapII	CCGG	4 cut(s)	44, 100, 372, 757
HgaI	GACGC	2 cut(s)	232, 399
HhaI	GCGC	1 cut(s)	810
Hin1II	CATG	9 cut(s)	39, 281, 695, 741, 1109, 1114, 1144, 1183, 1213
Hin6I	GCGC	1 cut(s)	808
HinP1I	GCGC	1 cut(s)	808
HincII	GTYRAC	1 cut(s)	15
HindII	GTYRAC	1 cut(s)	15
HindIII	AAGCTT	1 cut(s)	561
HinfI	GANTC	3 cut(s)	591, 749, 895
HpaII	CCGG	4 cut(s)	44, 100, 372, 757
HphI	GGTGA	1 cut(s)	1051
Hpy166II	GTNNAC	2 cut(s)	15, 1169
Hpy188I	TCNGA	7 cut(s)	91, 204, 590, 788, 900, 971, 1024
Hpy188III	TCNNGA	3 cut(s)	44, 414, 1106
Hpy8I	GTNNAC	2 cut(s)	15, 1169
Hpy99I	CGWCG	1 cut(s)	16
HpyAV	CCTTC	3 cut(s)	398, 486, 1140
HpyCH4III	ACNGT	1 cut(s)	317
HpyCH4IV	ACGT	1 cut(s)	172
HpyCH4V	TGCA	4 cut(s)	110, 117, 277, 573
HpyF10VI	GCNNNNNNNGC	5 cut(s)	32, 190, 416, 425, 1183
HpyF3I	CTNAG	3 cut(s)	19, 207, 899
HpySE526I	ACGT	1 cut(s)	172
Hsp92II	CATG	9 cut(s)	39, 281, 695, 741, 1109, 1114, 1144, 1183, 1213
HspAI	GCGC	1 cut(s)	808
Kpn2I	TCCGGA	1 cut(s)	43
Kzo9I	GATC	5 cut(s)	577, 585, 763, 880, 1162
Lsp1109I	GCAGC	4 cut(s)	163, 228, 287, 412
LweI	GCATC	2 cut(s)	163, 967
MaeI	CTAG	3 cut(s)	68, 414, 743
MaeII	ACGT	1 cut(s)	172
MaeIII	GTNAC	6 cut(s)	138, 173, 244, 349, 521, 671
MalI	GATC	5 cut(s)	579, 587, 765, 882, 1164
MboI	GATC	5 cut(s)	577, 585, 763, 880, 1162
MboII	GAAGA	5 cut(s)	17, 300, 801, 918, 1111
MflI	RGATCY	1 cut(s)	763
MhlI	GDGCHC	1 cut(s)	285
MluCI	AATT	4 cut(s)	340, 796, 1045, 1191
MlyI	GAGTC	1 cut(s)	889
MmeI	TCCRAC	1 cut(s)	227
MroI	TCCGGA	1 cut(s)	43
MseI	TTAA	5 cut(s)	359, 450, 546, 660, 915
MslI	CAYNNNNRTG	1 cut(s)	1211
MspI	CCGG	4 cut(s)	44, 100, 372, 757
MspR9I	CCNGG	4 cut(s)	373, 720, 757, 758
MvaI	CCWGG	1 cut(s)	720
MvnI	CGCG	1 cut(s)	410
MwoI	GCNNNNNNNGC	5 cut(s)	32, 190, 416, 425, 1183
NciI	CCSGG	3 cut(s)	373, 757, 758
NcoI	CCATGG	1 cut(s)	1209
NdeII	GATC	5 cut(s)	577, 585, 763, 880, 1162
NlaIII	CATG	9 cut(s)	39, 281, 695, 741, 1109, 1114, 1144, 1183, 1213
NlaIV	GGNNCC	1 cut(s)	201
NmuCI	GTSAC	3 cut(s)	173, 244, 671
NspI	RCATGY	3 cut(s)	281, 741, 1183
OliI	CACNNNNGTG	1 cut(s)	1211
PagI	TCATGA	1 cut(s)	1105
PfeI	GAWTC	2 cut(s)	591, 749
PkrI	GCNGC	5 cut(s)	153, 218, 302, 427, 1185
PleI	GAGTC	1 cut(s)	889
PpsI	GAGTC	1 cut(s)	889
PshAI	GACNNNNGTC	1 cut(s)	20
PshBI	ATTAAT	1 cut(s)	660
Psp6I	CCWGG	1 cut(s)	718
PspFI	CCCAGC	1 cut(s)	30
PspGI	CCWGG	1 cut(s)	718
PspN4I	GGNNCC	1 cut(s)	201
PspPI	GGNCC	1 cut(s)	1100
PsuI	RGATCY	1 cut(s)	763
RsaI	GTAC	4 cut(s)	379, 708, 724, 994
RsaNI	GTAC	4 cut(s)	378, 707, 723, 993
RseI	CAYNNNNRTG	1 cut(s)	1211
SalI	GTCGAC	1 cut(s)	13
SaqAI	TTAA	5 cut(s)	359, 450, 546, 660, 915
SatI	GCNGC	5 cut(s)	152, 217, 301, 426, 1184
Sau3AI	GATC	5 cut(s)	577, 585, 763, 880, 1162
Sau96I	GGNCC	1 cut(s)	1100
SchI	GAGTC	1 cut(s)	889
ScrFI	CCNGG	4 cut(s)	373, 720, 757, 758
SduI	GDGCHC	1 cut(s)	285
SfaNI	GCATC	2 cut(s)	163, 967
SfcI	CTRYAG	1 cut(s)	286
SmaI	CCCGGG	1 cut(s)	758
SmiMI	CAYNNNNRTG	1 cut(s)	1211
Sse9I	AATT	4 cut(s)	340, 796, 1045, 1191
SsiI	CCGC	3 cut(s)	303, 408, 1184
SspMI	CTAG	3 cut(s)	68, 414, 743
StyD4I	CCNGG	4 cut(s)	371, 718, 755, 756
StyI	CCWWGG	1 cut(s)	1209
TaaI	ACNGT	1 cut(s)	317
TaiI	ACGT	1 cut(s)	175
TaqI	TCGA	6 cut(s)	14, 333, 594, 656, 1004, 1122
TasI	AATT	4 cut(s)	340, 796, 1045, 1191
TauI	GCSGC	1 cut(s)	1186
TfiI	GAWTC	2 cut(s)	591, 749
Tru1I	TTAA	5 cut(s)	359, 450, 546, 660, 915
Tru9I	TTAA	5 cut(s)	359, 450, 546, 660, 915
TscAI	CASTG	1 cut(s)	652
TseFI	GTSAC	3 cut(s)	173, 244, 671
TseI	GCWGC	4 cut(s)	151, 216, 300, 425
Tsp45I	GTSAC	3 cut(s)	173, 244, 671
TspDTI	ATGAA	4 cut(s)	468, 513, 573, 830
TspGWI	ACGGA	2 cut(s)	540, 905
TspMI	CCCGGG	1 cut(s)	756
TspRI	CASTG	1 cut(s)	652
VspI	ATTAAT	1 cut(s)	660
XapI	RAATTY	1 cut(s)	1045
XbaI	TCTAGA	1 cut(s)	413
XceI	RCATGY	3 cut(s)	281, 741, 1183
XmaI	CCCGGG	1 cut(s)	756
XmiI	GTMKAC	1 cut(s)	14
XspI	CTAG	3 cut(s)	68, 414, 743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.