Rh5CG274700

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003266
Physical Location & Seq
Forward (+)
29690223 .. 29691404
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG274700.1

Sequence Viewer

Length: 1182 bp
ATGGTAGAGATAATGACCAAAAATCAAAATACGAGCACCCGGGAGAAGGGAATATTAACCACATGTTCAGTTTGGGAATCGCTTCAAAGGGACCTTCTGGATTTGATACTAGACAAGTTGTTAAAAATCGATGACTACATTCGGTTTGGTGTGGTTTGCCGCAAGCATTGGCAATTAGTTGCTTTAAATCAGCAGCAGCAACGCTTCGGATCACGTCACAAACAGCCTCCTCTAATGATTATTACAGAACAACAACAGAGCGGCGAAAGAAGCATGACCTTGCGTAGTGCTACACAGGACAAGACTTACAAGTTTCAGCTGAAACTGAATAATGTACCTAATTACGATAACATGAAGTATTGTGAATCTTCCCATGGTTGGTTGGCTTATGCACAAGGCAATTTGGTAGCAATAACCCTCTTAAACCCTTTCACCGGACGCACCATCACCCTTCCACCGGTACCAGCAACCGTTCCACCAGTACGAGCAACAGGCTCCTACTACTTCAAGAAAGTTGTACTGTCTGCTGACCCTTCTTCGTTCCCTGATGATTATCAAGTTCTAGTAATTTATAACGAGCGTGTTTATAATGCTGACACCAGGATAGCCCATTCTAAGTCGGGGGAAGATACTTGGACTCTCATAGACAGTGGGACGACTATCAGATTCACACATGCAGTTTGCTACAAAGGTCAATTTCTAGTTTGTGGTGGCACAAATGTTGATTTTTCGGTTTTTGCGGTTGATGTCAGCAGAAAAAGAATTACTCAACTCATGTATGGTGAACAATTTGTGCGCCGACATGAGGTTATGAATACATACCTTGTGGAATCGTCAAAGGGAGATCTATTGATGGTTAGGAGGTTTCACTTGCTTGATATTGATCGTTGTCTGATCGTCACGGTTGATAAATTGATATATGATTTTGGTGATGAACTCACACCCATACCCATTGAGAGTATTGGAAGTGATGCTTTGTTTTTGGATAAAAATCAATCTATTTGTGTGTCTGCCTTGGACTTTCCAGAGTGTCATCCAAATTCTATATACTTCACCGATGGTATTTTCAGCTTGGAAAGCAGACAAATGCATTGGTTTGAATCCTTAAAGCCAACAATGTCAGCAATATGGATTTCGCCAACGTTATGTGTTGAAAACCAACCTCTACCAACATGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

393

Amino Acids

44.93

Weight (kDa)

6.51

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 96 - 364 3.5e-43 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 573, 588
Acc65I GGTACC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 460
AccBSI CCGCTC 1 cut(s) 261
AciI CCGC 3 cut(s) 160, 261, 740
AclI AACGTT 1 cut(s) 1142
AclWI GGATC 1 cut(s) 217
AcsI RAATTY 1 cut(s) 1039
AfaI GTAC 4 cut(s) 336, 462, 483, 519
AfiI CCNNNNNNNGG 5 cut(s) 46, 378, 434, 457, 805
AflIII ACRYGT 1 cut(s) 62
AgeI ACCGGT 1 cut(s) 457
AgsI TTSAA 4 cut(s) 86, 508, 1100, 1154
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 2 cut(s) 319, 1071
AluI AGCT 2 cut(s) 319, 1071
Alw21I GWGCWC 1 cut(s) 38
AlwI GGATC 1 cut(s) 217
Ama87I CYCGRG 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 193, 196
ApoI RAATTY 1 cut(s) 1039
AsiGI ACCGGT 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 81
Asp718I GGTACC 1 cut(s) 460
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 2 cut(s) 40, 41
AsuHPI GGTGA 5 cut(s) 424, 439, 794, 941, 1045
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 205, 208
BccI CCATC 3 cut(s) 452, 847, 1052
BciT130I CCWGG 1 cut(s) 601
BcnI CCSGG 2 cut(s) 40, 41
BfaI CTAG 3 cut(s) 110, 563, 701
BglII AGATCT 1 cut(s) 844
BisI GCNGC 4 cut(s) 160, 194, 197, 262
BlsI GCNGC 4 cut(s) 161, 195, 198, 263
Bme1390I CCNGG 3 cut(s) 40, 41, 601
Bme18I GGWCC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 39
BmgBI CACGTC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 3 cut(s) 92, 462, 496
BmrFI CCNGG 3 cut(s) 40, 41, 601
BmsI GCATC 1 cut(s) 961
BpuMI CCSGG 2 cut(s) 40, 41
Bsa29I ATCGAT 1 cut(s) 129
BsaBI GATNNNNATC 3 cut(s) 552, 882, 990
BsaJI CCNNGG 3 cut(s) 39, 373, 1014
BsaWI WCCGGW 2 cut(s) 434, 457
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 378, 434, 457, 805
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 479
Bse8I GATNNNNATC 3 cut(s) 552, 882, 990
BseBI CCWGG 1 cut(s) 601
BseCI ATCGAT 1 cut(s) 129
BseDI CCNNGG 3 cut(s) 39, 373, 1014
BseGI GGATG 1 cut(s) 1033
BseJI GATNNNNATC 3 cut(s) 552, 882, 990
BseLI CCNNNNNNNGG 5 cut(s) 46, 378, 434, 457, 805
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 205, 208
BshNI GGYRCC 1 cut(s) 460
BshTI ACCGGT 1 cut(s) 457
BshVI ATCGAT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 3 cut(s) 40, 435, 458
BslFI GGGAC 2 cut(s) 104, 667
BslI CCNNNNNNNGG 5 cut(s) 46, 378, 434, 457, 805
BsmFI GGGAC 2 cut(s) 104, 667
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 4 cut(s) 209, 844, 883, 894
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 3 cut(s) 160, 261, 740
BspDI ATCGAT 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 92, 462, 496
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 460
BsrBI CCGCTC 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 457
BsrI ACTGG 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 39, 373, 1014
BssMI GATC 4 cut(s) 209, 844, 883, 894
BssT1I CCWWGG 2 cut(s) 373, 1014
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 4 cut(s) 472, 522, 650, 904
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 1 cut(s) 615
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 1033
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 4 cut(s) 212, 847, 886, 897
BstMBI GATC 4 cut(s) 209, 844, 883, 894
BstMWI GCNNNNNNNGC 2 cut(s) 270, 1077
BstNI CCWGG 1 cut(s) 601
BstNSI RCATGY 3 cut(s) 66, 677, 1176
BstSCI CCNGG 3 cut(s) 38, 39, 599
BstV1I GCAGC 2 cut(s) 205, 208
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 373
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 1 cut(s) 1033
BtsIMutI CAGTG 1 cut(s) 655
Cac8I GCNNGC 1 cut(s) 164
CfoI GCGC 1 cut(s) 798
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 91
Cfr9I CCCGGG 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 129
CseI GACGC 1 cut(s) 447
Csp6I GTAC 4 cut(s) 335, 461, 482, 518
CspAI ACCGGT 1 cut(s) 457
CviAII CATG 8 cut(s) 63, 274, 352, 374, 674, 775, 803, 1173
CviJI RGCY 7 cut(s) 226, 319, 386, 495, 608, 1071, 1111
CviKI_1 RGCY 7 cut(s) 226, 319, 386, 495, 608, 1071, 1111
CviQI GTAC 4 cut(s) 335, 461, 482, 518
DdeI CTNAG 1 cut(s) 615
DpnI GATC 4 cut(s) 211, 846, 885, 896
DpnII GATC 4 cut(s) 209, 844, 883, 894
DraI TTTAAA 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 373, 1014
Eco47I GGWCC 1 cut(s) 91
Eco88I CYCGRG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 2 cut(s) 373, 1014
EcoT22I ATGCAT 1 cut(s) 1092
ErhI CCWWGG 2 cut(s) 373, 1014
FaeI CATG 8 cut(s) 66, 277, 355, 377, 677, 778, 806, 1176
FalI AAGNNNNNCTT 2 cut(s) 958, 990
FaqI GGGAC 2 cut(s) 104, 667
FatI CATG 8 cut(s) 62, 273, 351, 373, 673, 774, 802, 1172
Fnu4HI GCNGC 4 cut(s) 160, 194, 197, 262
FokI GGATG 1 cut(s) 1020
Fsp4HI GCNGC 4 cut(s) 160, 194, 197, 262
FspBI CTAG 3 cut(s) 110, 563, 701
GlaI GCGC 1 cut(s) 797
GluI GCNGC 4 cut(s) 160, 194, 197, 262
HapII CCGG 3 cut(s) 40, 435, 458
HgaI GACGC 1 cut(s) 447
HhaI GCGC 1 cut(s) 798
Hin1II CATG 8 cut(s) 66, 277, 355, 377, 677, 778, 806, 1176
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HinfI GANTC 6 cut(s) 77, 365, 637, 666, 830, 1100
HpaII CCGG 3 cut(s) 40, 435, 458
HphI GGTGA 5 cut(s) 424, 439, 794, 941, 1045
Hpy166II GTNNAC 1 cut(s) 785
Hpy188I TCNGA 3 cut(s) 209, 665, 894
Hpy188III TCNNGA 3 cut(s) 98, 508, 1025
Hpy8I GTNNAC 1 cut(s) 785
HpyAV CCTTC 4 cut(s) 40, 104, 461, 543
HpyCH4III ACNGT 4 cut(s) 472, 522, 650, 904
HpyCH4IV ACGT 2 cut(s) 214, 1142
HpyCH4V TGCA 4 cut(s) 392, 677, 1090, 1176
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 1077
HpyF3I CTNAG 1 cut(s) 615
HpySE526I ACGT 2 cut(s) 214, 1142
Hsp92II CATG 8 cut(s) 66, 277, 355, 377, 677, 778, 806, 1176
HspAI GCGC 1 cut(s) 796
KpnI GGTACC 1 cut(s) 464
Kzo9I GATC 4 cut(s) 209, 844, 883, 894
LmnI GCTCC 1 cut(s) 500
Lsp1109I GCAGC 2 cut(s) 205, 208
LweI GCATC 1 cut(s) 961
MaeI CTAG 3 cut(s) 110, 563, 701
MaeII ACGT 2 cut(s) 214, 1142
MaeIII GTNAC 2 cut(s) 215, 898
MalI GATC 4 cut(s) 211, 846, 885, 896
MbiI CCGCTC 1 cut(s) 261
MboI GATC 4 cut(s) 209, 844, 883, 894
MboII GAAGA 3 cut(s) 360, 528, 638
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 9 cut(s) 173, 340, 400, 567, 695, 762, 788, 911, 1039
MlyI GAGTC 1 cut(s) 631
MnlI CCTC 6 cut(s) 237, 240, 428, 799, 855, 1173
Mph1103I ATGCAT 1 cut(s) 1092
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 5 cut(s) 56, 122, 185, 422, 1106
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 3 cut(s) 40, 435, 458
MspR9I CCNGG 3 cut(s) 40, 41, 601
MvaI CCWGG 1 cut(s) 601
MwoI GCNNNNNNNGC 2 cut(s) 270, 1077
NciI CCSGG 2 cut(s) 40, 41
NcoI CCATGG 1 cut(s) 373
NdeII GATC 4 cut(s) 209, 844, 883, 894
NlaIII CATG 8 cut(s) 66, 277, 355, 377, 677, 778, 806, 1176
NlaIV GGNNCC 3 cut(s) 92, 462, 496
NmuCI GTSAC 2 cut(s) 215, 898
NsiI ATGCAT 1 cut(s) 1092
NspI RCATGY 3 cut(s) 66, 677, 1176
PciI ACATGT 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 5 cut(s) 77, 365, 666, 830, 1100
PinAI ACCGGT 1 cut(s) 457
PkrI GCNGC 4 cut(s) 161, 195, 198, 263
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PpuMI RGGWCCY 1 cut(s) 91
PscI ACATGT 1 cut(s) 62
PsiI TTATAA 2 cut(s) 573, 588
Psp1406I AACGTT 1 cut(s) 1142
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 3 cut(s) 92, 462, 496
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 1 cut(s) 844
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 4 cut(s) 336, 462, 483, 519
RsaNI GTAC 4 cut(s) 335, 461, 482, 518
SaqAI TTAA 5 cut(s) 56, 122, 185, 422, 1106
SatI GCNGC 4 cut(s) 160, 194, 197, 262
Sau3AI GATC 4 cut(s) 209, 844, 883, 894
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 631
ScrFI CCNGG 3 cut(s) 40, 41, 601
SduI GDGCHC 1 cut(s) 38
SfaNI GCATC 1 cut(s) 961
SinI GGWCC 1 cut(s) 91
SmaI CCCGGG 1 cut(s) 41
Sse9I AATT 9 cut(s) 173, 340, 400, 567, 695, 762, 788, 911, 1039
SsiI CCGC 3 cut(s) 160, 261, 740
SspI AATATT 1 cut(s) 54
SspMI CTAG 3 cut(s) 110, 563, 701
StyD4I CCNGG 3 cut(s) 38, 39, 599
StyI CCWWGG 2 cut(s) 373, 1014
TaaI ACNGT 4 cut(s) 472, 522, 650, 904
TaiI ACGT 2 cut(s) 217, 1145
TaqI TCGA 1 cut(s) 129
TasI AATT 9 cut(s) 173, 340, 400, 567, 695, 762, 788, 911, 1039
TatI WGTACW 1 cut(s) 517
TauI GCSGC 2 cut(s) 162, 264
TfiI GAWTC 5 cut(s) 77, 365, 666, 830, 1100
Tru1I TTAA 5 cut(s) 56, 122, 185, 422, 1106
Tru9I TTAA 5 cut(s) 56, 122, 185, 422, 1106
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 2 cut(s) 215, 898
TseI GCWGC 2 cut(s) 193, 196
Tsp45I GTSAC 2 cut(s) 215, 898
TspDTI ATGAA 3 cut(s) 368, 827, 948
TspMI CCCGGG 1 cut(s) 39
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 1039
XceI RCATGY 3 cut(s) 66, 677, 1176
XmaI CCCGGG 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 3 cut(s) 110, 563, 701
Zsp2I ATGCAT 1 cut(s) 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.