Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002423

Physical Location & Seq

Genomic Coordinates Forward (+)

45701204 .. 45706531

Gene Structure 5328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1333900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 924 bp

ATGTTGAGGATGAACTTTATCAACTTTCGACCGATGAACTGCCCCTATCATATCTTGGTTTACAAAAGGAAGCCTCACTCATATCATGAATTTCCTATTCGAGTTGCTGAGTTGGGAACCGTATATAGATACGAATTATGTGGAAGCTTGCATGGTCTTTTTTGTGTAAGAGGTTTTACTCAGATCAAAGCTGAAATTAGGGGTGTCCTAGATCTTACCGAAGAACTTTTGTTGCAATTTGGCTTCAACAAATATGAGGTCAATCTCTCTACGAGGCCAGAGAAAGCTGTTGGAGATGATGATATATGGATTGATCTTAAGATTGAGGATGCCCTTGGAAGGAAGTGGCAGTGCTCAACCATACAGGTTGATTTTAACCTACCACAGCGTTTTGACTTAACATATGCTGACTCAAACTCTGAAAAGAAGCGGCCTATCATGATTCATAGAGCTGTTCTTGGGTCATTGGAGCGATTTTTTTGGGTCCTTATAGAGCATTATGCCGGGGATTTTCCATTATGGCTTTCGCCAGTACAAGCTCATGTTTTACCAATTACTGACTCTCAGGTTTGTAAAGCAGTTGCACGTTTAATGTTTCAATTAACTCAAGAACCCTCAACACACCTGCACGACGGTTACCTGGGCTACGACCCCCGACTCCTCCTCCCAGCGATTCGACTCCCCTCCGAGTCGCTCATGGACTCCGCCACCGATTCCCCAATCTTCCACGGCTCCACCGTAGACGACACTTTTGCCACTCAAACGGCGTCGGAGGCCTTCTCTCCTCCATCGATCTACGCCAAATCGAACGGCCAGGGCTTTCATGGAGGGTTTGGCGGGTTCGACGATCCACTTTTTCCGGCTCCATCGGAGATGCTGCCTGAGGAGGGCTTCGCTCTCAGAGAATGGAGGAGGCTTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_2b tRNA_SAD

Protein Analysis

308

Amino Acids

35.1

Weight (kDa)

5.77

Isoelectric Point (pI)

48.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2b	PF00587	9 - 167	1.5e-28	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40060
fragaria_vesca	FvH4_4g08020 FvH4_7g24260
malus_domestica	MD00G1024200.v1.1 MD00G1047100.v1.1 MD00G1047900.v1.1 MD00G1048400.v1.1 MD00G1062700.v1.1 MD01G1132200.v1.1 MD01G1150700.v1.1 MD01G1152000.v1.1 MD05G1333900.v1.1 MD07G1216800.v1.1 MD07G1219900.v1.1
prunus_persica	Prupe.2G248500_v2.0.a1
pyrus_communis	pycom01g16830 pycom01g16850 pycom01g16880 pycom07g19900 pycom11g19450
rosa_chinensis	RchiOBHm_Chr1g0369851
rosa_laevigata	RLG00000027088 RLG00000027098
rosa_multiflora	Rmu_co8474619.1_g000001 Rmu_sc0008140.1_g000010
rosa_roxburghii	Rroxscaffold_3G00232620 Rroxscaffold_4G00286940 Rroxscaffold_4G00287110 Rroxscaffold_4G00322920
rosa_rugosa	Rorug01G0352700
rosa_samantha	Rh1AG194100 Rh1AG361200 Rh1BG178100 Rh1BG324900 Rh1CG179000 Rh1CG338100 Rh1DG087000 Rh1DG207800 Rh1DG355300
rosa_wichuraiana	Rw0G015470 Rw1G031810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	633
Acc36I	ACCTGC	1 cut(s)	633
AccI	GTMKAC	1 cut(s)	741
AciI	CCGC	3 cut(s)	430, 705, 837
AclWI	GGATC	1 cut(s)	842
AcoI	YGGCCR	1 cut(s)	811
AcsI	RAATTY	1 cut(s)	89
AcyI	GRCGYC	1 cut(s)	767
AfaI	GTAC	1 cut(s)	534
AfiI	CCNNNNNNNGG	2 cut(s)	339, 887
AflII	CTTAAG	1 cut(s)	317
AgsI	TTSAA	2 cut(s)	247, 599
AjnI	CCWGG	2 cut(s)	639, 813
AluBI	AGCT	5 cut(s)	147, 191, 287, 452, 539
AluI	AGCT	5 cut(s)	147, 191, 287, 452, 539
Alw21I	GWGCWC	1 cut(s)	356
AlwI	GGATC	1 cut(s)	842
AoxI	GGCC	4 cut(s)	275, 431, 774, 811
ApeKI	GCWGC	1 cut(s)	877
ApoI	RAATTY	1 cut(s)	89
ArsI	GACNNNNNNTTYG	4 cut(s)	243, 275, 734, 766
AspS9I	GGNCC	1 cut(s)	484
AsuC2I	CCSGG	1 cut(s)	505
AvaII	GGWCC	1 cut(s)	484
AxyI	CCTNAGG	1 cut(s)	882
Bbv12I	GWGCWC	1 cut(s)	356
BbvI	GCAGC	1 cut(s)	864
BccI	CCATC	2 cut(s)	796, 874
BceAI	ACGGC	3 cut(s)	745, 780, 826
BcgI	CGANNNNNNTGC	2 cut(s)	734, 768
BciT130I	CCWGG	2 cut(s)	641, 815
BcnI	CCSGG	1 cut(s)	505
BfaI	CTAG	1 cut(s)	209
BfrI	CTTAAG	1 cut(s)	317
BfuAI	ACCTGC	1 cut(s)	633
BglII	AGATCT	1 cut(s)	211
BisI	GCNGC	2 cut(s)	431, 878
BlsI	GCNGC	2 cut(s)	432, 879
Bme1390I	CCNGG	3 cut(s)	505, 641, 815
Bme18I	GGWCC	1 cut(s)	484
BmgT120I	GGNCC	1 cut(s)	484
BmiI	GGNNCC	4 cut(s)	118, 485, 733, 864
BmrFI	CCNGG	3 cut(s)	505, 641, 815
BmsI	GCATC	2 cut(s)	319, 864
BplI	GAGNNNNNCTC	2 cut(s)	764, 796
BpuEI	CTTGAG	1 cut(s)	591
BpuMI	CCSGG	1 cut(s)	505
Bsa29I	ATCGAT	1 cut(s)	791
BsaHI	GRCGYC	1 cut(s)	767
BsaJI	CCNNGG	5 cut(s)	334, 504, 640, 727, 814
BsaXI	ACNNNNNCTCC	2 cut(s)	648, 678
Bsc4I	CCNNNNNNNGG	2 cut(s)	339, 887
Bse1I	ACTGG	1 cut(s)	530
Bse21I	CCTNAGG	1 cut(s)	882
BseBI	CCWGG	2 cut(s)	641, 815
BseCI	ATCGAT	1 cut(s)	791
BseDI	CCNNGG	5 cut(s)	334, 504, 640, 727, 814
BseGI	GGATG	2 cut(s)	15, 334
BseLI	CCNNNNNNNGG	2 cut(s)	339, 887
BseMII	CTCAG	5 cut(s)	99, 194, 578, 873, 913
BseNI	ACTGG	1 cut(s)	530
BseRI	GAGGAG	4 cut(s)	650, 653, 774, 899
BseXI	GCAGC	1 cut(s)	864
BseYI	CCCAGC	1 cut(s)	667
BsgI	GTGCAG	1 cut(s)	611
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	4 cut(s)	277, 433, 776, 813
BshVI	ATCGAT	1 cut(s)	791
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	1 cut(s)	356
BsiSI	CCGG	2 cut(s)	504, 860
BslI	CCNNNNNNNGG	2 cut(s)	339, 887
BsnI	GGCC	4 cut(s)	277, 433, 776, 813
Bsp1286I	GDGCHC	1 cut(s)	356
Bsp143I	GATC	5 cut(s)	183, 211, 313, 792, 847
BspACI	CCGC	3 cut(s)	430, 705, 837
BspANI	GGCC	4 cut(s)	277, 433, 776, 813
BspCNI	CTCAG	5 cut(s)	100, 193, 577, 874, 912
BspDI	ATCGAT	1 cut(s)	791
BspHI	TCATGA	2 cut(s)	85, 438
BspLI	GGNNCC	4 cut(s)	118, 485, 733, 864
BspMI	ACCTGC	1 cut(s)	633
BspPI	GGATC	1 cut(s)	842
BspTI	CTTAAG	1 cut(s)	317
BsrI	ACTGG	1 cut(s)	530
BssECI	CCNNGG	5 cut(s)	334, 504, 640, 727, 814
BssMI	GATC	5 cut(s)	183, 211, 313, 792, 847
BssNI	GRCGYC	1 cut(s)	767
BssT1I	CCWWGG	1 cut(s)	334
Bst2UI	CCWGG	2 cut(s)	641, 815
Bst4CI	ACNGT	3 cut(s)	121, 635, 739
BstACI	GRCGYC	1 cut(s)	767
BstAFI	CTTAAG	1 cut(s)	317
BstC8I	GCNNGC	1 cut(s)	149
BstDEI	CTNAG	5 cut(s)	108, 180, 564, 882, 899
BstDSI	CCRYGG	1 cut(s)	727
BstEII	GGTNACC	1 cut(s)	635
BstF5I	GGATG	2 cut(s)	15, 334
BstKTI	GATC	5 cut(s)	186, 214, 316, 795, 850
BstMBI	GATC	5 cut(s)	183, 211, 313, 792, 847
BstMCI	CGRYCG	1 cut(s)	32
BstMWI	GCNNNNNNNGC	1 cut(s)	773
BstNI	CCWGG	2 cut(s)	641, 815
BstPI	GGTNACC	1 cut(s)	635
BstSCI	CCNGG	3 cut(s)	503, 639, 813
BstV1I	GCAGC	1 cut(s)	864
BstX2I	RGATCY	1 cut(s)	211
BstYI	RGATCY	1 cut(s)	211
Bsu15I	ATCGAT	1 cut(s)	791
Bsu36I	CCTNAGG	1 cut(s)	882
BsuRI	GGCC	4 cut(s)	277, 433, 776, 813
BsuTUI	ATCGAT	1 cut(s)	791
BtgI	CCRYGG	1 cut(s)	727
BtsCI	GGATG	2 cut(s)	15, 334
BtsI	GCAGTG	1 cut(s)	356
BtsIMutI	CAGTG	1 cut(s)	356
BveI	ACCTGC	1 cut(s)	633
Cac8I	GCNNGC	1 cut(s)	149
CciI	TCATGA	2 cut(s)	85, 438
Cfr13I	GGNCC	1 cut(s)	484
ClaI	ATCGAT	1 cut(s)	791
CseI	GACGC	1 cut(s)	756
Csp6I	GTAC	1 cut(s)	533
CviAII	CATG	6 cut(s)	86, 152, 439, 542, 697, 824
CviQI	GTAC	1 cut(s)	533
DdeI	CTNAG	5 cut(s)	108, 180, 564, 882, 899
DpnI	GATC	5 cut(s)	185, 213, 315, 794, 849
DpnII	GATC	5 cut(s)	183, 211, 313, 792, 847
EaeI	YGGCCR	1 cut(s)	811
EciI	GGCGGA	1 cut(s)	694
Eco130I	CCWWGG	1 cut(s)	334
Eco147I	AGGCCT	1 cut(s)	776
Eco47I	GGWCC	1 cut(s)	484
Eco81I	CCTNAGG	1 cut(s)	882
Eco91I	GGTNACC	1 cut(s)	635
EcoO109I	RGGNCCY	1 cut(s)	484
EcoO65I	GGTNACC	1 cut(s)	635
EcoRII	CCWGG	2 cut(s)	639, 813
EcoT14I	CCWWGG	1 cut(s)	334
ErhI	CCWWGG	1 cut(s)	334
FaeI	CATG	6 cut(s)	89, 155, 442, 545, 700, 827
FatI	CATG	6 cut(s)	85, 151, 438, 541, 696, 823
FauI	CCCGC	1 cut(s)	830
FauNDI	CATATG	1 cut(s)	403
FblI	GTMKAC	1 cut(s)	741
Fnu4HI	GCNGC	2 cut(s)	431, 878
FokI	GGATG	2 cut(s)	22, 341
Fsp4HI	GCNGC	2 cut(s)	431, 878
FspBI	CTAG	1 cut(s)	209
GluI	GCNGC	2 cut(s)	431, 878
GsaI	CCCAGC	1 cut(s)	671
HaeIII	GGCC	4 cut(s)	277, 433, 776, 813
HapII	CCGG	2 cut(s)	504, 860
HgaI	GACGC	1 cut(s)	756
Hin1I	GRCGYC	1 cut(s)	767
Hin1II	CATG	6 cut(s)	89, 155, 442, 545, 700, 827
HindIII	AAGCTT	1 cut(s)	145
HinfI	GANTC	9 cut(s)	410, 442, 560, 657, 673, 678, 689, 701, 713
HpaII	CCGG	2 cut(s)	504, 860
Hpy166II	GTNNAC	2 cut(s)	61, 742
Hpy188I	TCNGA	7 cut(s)	183, 421, 688, 772, 871, 902, 923
Hpy188III	TCNNGA	3 cut(s)	86, 439, 608
Hpy8I	GTNNAC	2 cut(s)	61, 742
Hpy99I	CGWCG	3 cut(s)	635, 772, 848
HpyAV	CCTTC	2 cut(s)	333, 787
HpyCH4III	ACNGT	3 cut(s)	121, 635, 739
HpyCH4IV	ACGT	1 cut(s)	586
HpyCH4V	TGCA	4 cut(s)	151, 235, 584, 628
HpyF10VI	GCNNNNNNNGC	1 cut(s)	773
HpyF3I	CTNAG	5 cut(s)	108, 180, 564, 882, 899
HpySE526I	ACGT	1 cut(s)	586
Hsp92I	GRCGYC	1 cut(s)	767
Hsp92II	CATG	6 cut(s)	89, 155, 442, 545, 700, 827
Kzo9I	GATC	5 cut(s)	183, 211, 313, 792, 847
LmnI	GCTCC	3 cut(s)	469, 737, 868
Lsp1109I	GCAGC	1 cut(s)	864
LweI	GCATC	2 cut(s)	319, 864
MaeI	CTAG	1 cut(s)	209
MaeII	ACGT	1 cut(s)	586
MaeIII	GTNAC	1 cut(s)	635
MalI	GATC	5 cut(s)	185, 213, 315, 794, 849
MboI	GATC	5 cut(s)	183, 211, 313, 792, 847
MboII	GAAGA	2 cut(s)	233, 715
MflI	RGATCY	1 cut(s)	211
MhlI	GDGCHC	1 cut(s)	356
MluCI	AATT	6 cut(s)	89, 134, 195, 236, 552, 599
MlyI	GAGTC	6 cut(s)	404, 554, 651, 672, 695, 698
MmeI	TCCRAC	2 cut(s)	271, 750
MseI	TTAA	5 cut(s)	318, 375, 398, 590, 602
MspCI	CTTAAG	1 cut(s)	317
MspI	CCGG	2 cut(s)	504, 860
MspR9I	CCNGG	3 cut(s)	505, 641, 815
MvaI	CCWGG	2 cut(s)	641, 815
MwoI	GCNNNNNNNGC	1 cut(s)	773
NciI	CCSGG	1 cut(s)	505
NdeI	CATATG	1 cut(s)	403
NdeII	GATC	5 cut(s)	183, 211, 313, 792, 847
NlaIII	CATG	6 cut(s)	89, 155, 442, 545, 700, 827
NlaIV	GGNNCC	4 cut(s)	118, 485, 733, 864
PagI	TCATGA	2 cut(s)	85, 438
PaqCI	CACCTGC	1 cut(s)	633
PceI	AGGCCT	1 cut(s)	776
PcsI	WCGNNNNNNNCGW	1 cut(s)	735
PfeI	GAWTC	3 cut(s)	442, 673, 713
PkrI	GCNGC	2 cut(s)	432, 879
PleI	GAGTC	6 cut(s)	404, 554, 651, 672, 695, 697
PpsI	GAGTC	6 cut(s)	404, 554, 651, 672, 695, 697
PpuMI	RGGWCCY	1 cut(s)	484
Psp5II	RGGWCCY	1 cut(s)	484
Psp6I	CCWGG	2 cut(s)	639, 813
PspEI	GGTNACC	1 cut(s)	635
PspFI	CCCAGC	1 cut(s)	667
PspGI	CCWGG	2 cut(s)	639, 813
PspN4I	GGNNCC	4 cut(s)	118, 485, 733, 864
PspPI	GGNCC	1 cut(s)	484
PspPPI	RGGWCCY	1 cut(s)	484
PsuI	RGATCY	1 cut(s)	211
RsaI	GTAC	1 cut(s)	534
RsaNI	GTAC	1 cut(s)	533
SaqAI	TTAA	5 cut(s)	318, 375, 398, 590, 602
SatI	GCNGC	2 cut(s)	431, 878
Sau3AI	GATC	5 cut(s)	183, 211, 313, 792, 847
Sau96I	GGNCC	1 cut(s)	484
SchI	GAGTC	6 cut(s)	404, 554, 651, 672, 695, 698
ScrFI	CCNGG	3 cut(s)	505, 641, 815
SduI	GDGCHC	1 cut(s)	356
SfaNI	GCATC	2 cut(s)	319, 864
SinI	GGWCC	1 cut(s)	484
SmlI	CTYRAG	2 cut(s)	317, 606
SmoI	CTYRAG	2 cut(s)	317, 606
Sse9I	AATT	6 cut(s)	89, 134, 195, 236, 552, 599
SseBI	AGGCCT	1 cut(s)	776
SsiI	CCGC	3 cut(s)	430, 705, 837
SspMI	CTAG	1 cut(s)	209
StuI	AGGCCT	1 cut(s)	776
StyD4I	CCNGG	3 cut(s)	503, 639, 813
StyI	CCWWGG	1 cut(s)	334
TaaI	ACNGT	3 cut(s)	121, 635, 739
TaiI	ACGT	1 cut(s)	589
TaqI	TCGA	6 cut(s)	28, 100, 676, 791, 806, 843
TaqII	GACCGA	1 cut(s)	46
TasI	AATT	6 cut(s)	89, 134, 195, 236, 552, 599
TatI	WGTACW	1 cut(s)	532
TauI	GCSGC	1 cut(s)	433
TfiI	GAWTC	3 cut(s)	442, 673, 713
Tru1I	TTAA	5 cut(s)	318, 375, 398, 590, 602
Tru9I	TTAA	5 cut(s)	318, 375, 398, 590, 602
TscAI	CASTG	1 cut(s)	356
TseI	GCWGC	1 cut(s)	877
TspDTI	ATGAA	5 cut(s)	26, 50, 102, 434, 812
TspRI	CASTG	1 cut(s)	356
Vha464I	CTTAAG	1 cut(s)	317
VpaK11BI	GGWCC	1 cut(s)	484
XapI	RAATTY	1 cut(s)	89
XcmI	CCANNNNNNNNNTGG	1 cut(s)	821
XmiI	GTMKAC	1 cut(s)	741
XspI	CTAG	1 cut(s)	209

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.