Rorug01G0352700

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002423
Physical Location & Seq
Forward (+)
46980673 .. 46981527
855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0352700.1

Sequence Viewer

Length: 585 bp
ATGCAACGTGGTACCATGGCCGATCAGCTGTCCCCCGACCAGAAGTCAAAGTTCAAGGAGGCGTTCAGCCTTTTCGACAAGGACGGCGATGGCTCCATCACTACCAAAGAGCTCGGCACTGTGATGCGCTCACTTGGGCAGAACCCGACCGAAGAAGAGCTTCAAGATATGATCAACGAGGTTGATGCCGATGGAAGCGGTACCATGGAATGCCAAGAGTTCCTTGAACTGATGGCCAAAAAGATTAAGGGCACTGATTCCGATGAGGAGCTCAAGGAAGCGTTTCGGGTGTTTGATAAGGACCAGAATGGCTTCATTTCTGCCGCCGAGCTCCGTCATGTCCTGACGAATCTCGGCGAGAAGCTGACAGATGAGGAAATTGGTGAGATGGTTAGGGAGGCTGATTTGGACAGAGATGGACAAATCAACTATGAGGAGTTTGTCAAAGTTATGGTGGCCAAGAGAGGACGAAGCCGGAGCAGCGGGCACGGTAACATGAATTCTTCTTCAAGGGAACACAAGACTAAGAACGGCCGTACTAAACAGCGTATACGCCAATATATTTCTGCCTGTAGTGTAATGTAA

Protein Analysis

194

Amino Acids

21.87

Weight (kDa)

5.08

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 14 - 81 2.9e-07 Allograft inflammatory factor 1
EF-hand_7 PF13499 16 - 78 3.2e-16 EF-hand domain pair
EF-hand_1 PF00036 17 - 45 1.4e-08 EF hand domain
EF-hand_6 PF13405 17 - 46 7.8e-09 EF-hand domain
EF-hand_5 PF13202 19 - 42 9.9e-06 EF hand
EF-hand_9 PF14658 20 - 82 1.4e-09 EF-hand domain
EF-hand_8 PF13833 31 - 80 4.2e-08 EF-hand domain pair
EF-hand_1 PF00036 53 - 80 3e-06 EF hand domain
EF-hand_7 PF13499 89 - 151 9.8e-20 EF-hand domain pair
AIF-1 PF21008 89 - 155 3.4e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 90 - 117 1.1e-08 EF hand domain
EF-hand_6 PF13405 90 - 119 2.1e-09 EF-hand domain
EF-hand_5 PF13202 91 - 114 3.4e-06 EF hand
EF-hand_8 PF13833 103 - 152 7.6e-12 EF-hand domain pair
EF-hand_1 PF00036 126 - 152 2.3e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 11, 200
AccB1I GGYRCC 2 cut(s) 11, 200
AccI GTMKAC 1 cut(s) 550
AciI CCGC 3 cut(s) 198, 324, 483
AcoI YGGCCR 4 cut(s) 18, 234, 456, 532
AcsI RAATTY 1 cut(s) 499
AfaI GTAC 3 cut(s) 13, 202, 538
AgsI TTSAA 4 cut(s) 55, 164, 227, 510
AhdI GACNNNNNGTC 1 cut(s) 43
AluBI AGCT 6 cut(s) 28, 112, 160, 271, 331, 364
AluI AGCT 6 cut(s) 28, 112, 160, 271, 331, 364
Alw21I GWGCWC 3 cut(s) 114, 273, 333
AoxI GGCC 4 cut(s) 18, 234, 456, 532
ApeKI GCWGC 1 cut(s) 480
ApoI RAATTY 1 cut(s) 499
Asp700I GAANNNNTTC 3 cut(s) 159, 282, 311
Asp718I GGTACC 2 cut(s) 11, 200
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 2 cut(s) 254, 489
BalI TGGCCA 2 cut(s) 236, 458
BanI GGYRCC 2 cut(s) 11, 200
BanII GRGCYC 3 cut(s) 114, 273, 333
Bbv12I GWGCWC 3 cut(s) 114, 273, 333
BbvI GCAGC 1 cut(s) 492
BccI CCATC 6 cut(s) 83, 104, 185, 226, 382, 410
BceAI ACGGC 3 cut(s) 100, 519, 547
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 571
BisI GCNGC 2 cut(s) 324, 481
BlsI GCNGC 2 cut(s) 325, 482
Bme18I GGWCC 1 cut(s) 301
BmeRI GACNNNNNGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 3 cut(s) 13, 94, 202
BmsI GCATC 2 cut(s) 114, 175
BpuEI CTTGAG 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 15, 204
BseDI CCNNGG 2 cut(s) 15, 204
BseRI GAGGAG 2 cut(s) 281, 449
BseSI GKGCMC 2 cut(s) 254, 489
BseX3I CGGCCG 1 cut(s) 532
BseXI GCAGC 1 cut(s) 492
Bsh1285I CGRYCG 2 cut(s) 150, 535
BshFI GGCC 4 cut(s) 20, 236, 458, 534
BshNI GGYRCC 2 cut(s) 11, 200
BsiEI CGRYCG 2 cut(s) 150, 535
BsiHKAI GWGCWC 3 cut(s) 114, 273, 333
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 16
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 4 cut(s) 20, 236, 458, 534
Bsp1286I GDGCHC 5 cut(s) 114, 254, 273, 333, 489
Bsp143I GATC 2 cut(s) 22, 171
Bsp19I CCATGG 2 cut(s) 15, 204
BspACI CCGC 3 cut(s) 198, 324, 483
BspANI GGCC 4 cut(s) 20, 236, 458, 534
BspLI GGNNCC 3 cut(s) 13, 94, 202
BspQI GCTCTTC 1 cut(s) 150
BspT107I GGYRCC 2 cut(s) 11, 200
BssECI CCNNGG 2 cut(s) 15, 204
BssMI GATC 2 cut(s) 22, 171
BssNAI GTATAC 1 cut(s) 551
BssT1I CCWWGG 2 cut(s) 15, 204
Bst1107I GTATAC 1 cut(s) 551
Bst4CI ACNGT 2 cut(s) 121, 491
Bst6I CTCTTC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 1 cut(s) 525
BstDSI CCRYGG 2 cut(s) 15, 204
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 2 cut(s) 25, 174
BstMBI GATC 2 cut(s) 22, 171
BstMCI CGRYCG 2 cut(s) 150, 535
BstMWI GCNNNNNNNGC 1 cut(s) 480
BstSFI CTRYAG 1 cut(s) 571
BstSLI GKGCMC 2 cut(s) 254, 489
BstV1I GCAGC 1 cut(s) 492
BstZ17I GTATAC 1 cut(s) 551
BstZI CGGCCG 1 cut(s) 532
BsuRI GGCC 4 cut(s) 20, 236, 458, 534
BtgI CCRYGG 2 cut(s) 15, 204
BtgZI GCGATG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 117, 252
Cac8I GCNNGC 1 cut(s) 485
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 301
Csp6I GTAC 3 cut(s) 12, 201, 537
CviAII CATG 4 cut(s) 16, 205, 338, 496
CviQI GTAC 3 cut(s) 12, 201, 537
DdeI CTNAG 1 cut(s) 525
DpnI GATC 2 cut(s) 24, 173
DpnII GATC 2 cut(s) 22, 171
DriI GACNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 4 cut(s) 18, 234, 456, 532
EagI CGGCCG 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 150
Ecl136II GAGCTC 3 cut(s) 112, 271, 331
EclXI CGGCCG 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 15, 204
Eco24I GRGCYC 3 cut(s) 114, 273, 333
Eco47I GGWCC 1 cut(s) 301
Eco52I CGGCCG 1 cut(s) 532
Eco53kI GAGCTC 3 cut(s) 112, 271, 331
EcoICRI GAGCTC 3 cut(s) 112, 271, 331
EcoRI GAATTC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 15, 204
EcoT38I GRGCYC 3 cut(s) 114, 273, 333
ErhI CCWWGG 2 cut(s) 15, 204
FaeI CATG 4 cut(s) 19, 208, 341, 499
FaiI YATR 9 cut(s) 17, 170, 206, 339, 432, 452, 497, 551, 561
FalI AAGNNNNNCTT 4 cut(s) 144, 176, 207, 239
FaqI GGGAC 1 cut(s) 16
FatI CATG 4 cut(s) 15, 204, 337, 495
FauI CCCGC 1 cut(s) 476
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 324, 481
FriOI GRGCYC 3 cut(s) 114, 273, 333
Fsp4HI GCNGC 2 cut(s) 324, 481
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 324, 481
HaeIII GGCC 4 cut(s) 20, 236, 458, 534
HapII CCGG 1 cut(s) 475
HhaI GCGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 19, 208, 341, 499
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 2 cut(s) 257, 349
HpaII CCGG 1 cut(s) 475
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 1 cut(s) 551
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 164, 343
Hpy8I GTNNAC 1 cut(s) 551
HpyCH4III ACNGT 2 cut(s) 121, 491
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 480
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 4 cut(s) 19, 208, 341, 499
HspAI GCGC 1 cut(s) 127
KpnI GGTACC 2 cut(s) 15, 204
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 22, 171
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 4 cut(s) 98, 268, 336, 477
LpnPI CCDG 4 cut(s) 53, 317, 356, 488
Lsp1109I GCAGC 1 cut(s) 492
LweI GCATC 2 cut(s) 114, 175
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 491
MalI GATC 2 cut(s) 24, 173
MboI GATC 2 cut(s) 22, 171
MboII GAAGA 4 cut(s) 164, 167, 495, 498
MhlI GDGCHC 5 cut(s) 114, 254, 273, 333, 489
MlsI TGGCCA 2 cut(s) 236, 458
MluCI AATT 2 cut(s) 378, 499
MluNI TGGCCA 2 cut(s) 236, 458
MnlI CCTC 7 cut(s) 52, 172, 259, 367, 391, 427, 458
Mox20I TGGCCA 2 cut(s) 236, 458
MroXI GAANNNNTTC 3 cut(s) 159, 282, 311
MscI TGGCCA 2 cut(s) 236, 458
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 2 cut(s) 236, 458
MspA1I CMGCKG 2 cut(s) 28, 483
MspI CCGG 1 cut(s) 475
Mva1269I GAATGC 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 480
NcoI CCATGG 2 cut(s) 15, 204
NdeII GATC 2 cut(s) 22, 171
NlaIII CATG 4 cut(s) 19, 208, 341, 499
NlaIV GGNNCC 3 cut(s) 13, 94, 202
NmeAIII GCCGAG 3 cut(s) 93, 333, 352
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 1 cut(s) 215
PdmI GAANNNNTTC 3 cut(s) 159, 282, 311
PfeI GAWTC 2 cut(s) 257, 349
PkrI GCNGC 2 cut(s) 325, 482
Psp124BI GAGCTC 3 cut(s) 114, 273, 333
PspN4I GGNNCC 3 cut(s) 13, 94, 202
PspPI GGNCC 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 3 cut(s) 13, 202, 538
RsaNI GTAC 3 cut(s) 12, 201, 537
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 3 cut(s) 114, 273, 333
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 2 cut(s) 324, 481
Sau3AI GATC 2 cut(s) 22, 171
Sau96I GGNCC 1 cut(s) 301
SduI GDGCHC 5 cut(s) 114, 254, 273, 333, 489
SetI ASST 8 cut(s) 10, 30, 114, 162, 183, 273, 333, 366
SfaNI GCATC 2 cut(s) 114, 175
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 378, 499
SsiI CCGC 3 cut(s) 198, 324, 483
SstI GAGCTC 3 cut(s) 114, 273, 333
StyI CCWWGG 2 cut(s) 15, 204
TaaI ACNGT 2 cut(s) 121, 491
TaiI ACGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 75
TaqII GACCGA 1 cut(s) 164
TasI AATT 2 cut(s) 378, 499
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 257, 349
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 2 cut(s) 124, 259
TseI GCWGC 1 cut(s) 480
TspDTI ATGAA 2 cut(s) 304, 512
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 2 cut(s) 124, 259
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 550
XmnI GAANNNNTTC 3 cut(s) 159, 282, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.