Belongs to the WD repeat SEC13 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 350 |
| AccB1I | GGYRCC | 2 cut(s) | 32, 703 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 609 |
| AccI | GTMKAC | 2 cut(s) | 87, 482 |
| AccII | CGCG | 1 cut(s) | 117 |
| AciI | CCGC | 2 cut(s) | 315, 600 |
| AclWI | GGATC | 2 cut(s) | 413, 426 |
| AcoI | YGGCCR | 2 cut(s) | 61, 490 |
| AcsI | RAATTY | 3 cut(s) | 206, 455, 614 |
| AcuI | CTGAAG | 3 cut(s) | 291, 431, 452 |
| AfaI | GTAC | 1 cut(s) | 912 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 209, 400, 536, 609, 713, 790 |
| AgsI | TTSAA | 5 cut(s) | 287, 574, 579, 934, 965 |
| AjnI | CCWGG | 1 cut(s) | 546 |
| AluBI | AGCT | 5 cut(s) | 295, 306, 323, 412, 445 |
| AluI | AGCT | 5 cut(s) | 295, 306, 323, 412, 445 |
| Alw21I | GWGCWC | 2 cut(s) | 165, 302 |
| Alw26I | GTCTC | 1 cut(s) | 355 |
| Alw44I | GTGCAC | 2 cut(s) | 161, 298 |
| AlwI | GGATC | 2 cut(s) | 413, 426 |
| AoxI | GGCC | 3 cut(s) | 61, 490, 719 |
| ApaLI | GTGCAC | 2 cut(s) | 161, 298 |
| ApeKI | GCWGC | 2 cut(s) | 170, 442 |
| ApoI | RAATTY | 3 cut(s) | 206, 455, 614 |
| AspLEI | GCGC | 1 cut(s) | 22 |
| AsuHPI | GGTGA | 3 cut(s) | 345, 689, 742 |
| BaeGI | GKGCMC | 3 cut(s) | 165, 302, 706 |
| BalI | TGGCCA | 1 cut(s) | 492 |
| BamHI | GGATCC | 1 cut(s) | 418 |
| BanI | GGYRCC | 2 cut(s) | 32, 703 |
| BauI | CACGAG | 1 cut(s) | 164 |
| Bbv12I | GWGCWC | 2 cut(s) | 165, 302 |
| BbvI | GCAGC | 2 cut(s) | 157, 454 |
| BccI | CCATC | 7 cut(s) | 44, 110, 233, 630, 788, 850, 896 |
| BciT130I | CCWGG | 1 cut(s) | 548 |
| BclI | TGATCA | 2 cut(s) | 619, 679 |
| BcoDI | GTCTC | 1 cut(s) | 355 |
| BfaI | CTAG | 2 cut(s) | 332, 927 |
| BfmI | CTRYAG | 1 cut(s) | 483 |
| BfoI | RGCGCY | 1 cut(s) | 23 |
| BfuAI | ACCTGC | 1 cut(s) | 350 |
| BisI | GCNGC | 2 cut(s) | 171, 443 |
| BlsI | GCNGC | 2 cut(s) | 172, 444 |
| Bme1390I | CCNGG | 1 cut(s) | 548 |
| BmiI | GGNNCC | 7 cut(s) | 34, 82, 94, 198, 420, 641, 705 |
| BmrFI | CCNGG | 1 cut(s) | 548 |
| BmrI | ACTGGG | 1 cut(s) | 638 |
| BmsI | GCATC | 5 cut(s) | 205, 265, 379, 521, 778 |
| BmuI | ACTGGG | 1 cut(s) | 638 |
| BsaJI | CCNNGG | 3 cut(s) | 156, 423, 603 |
| BsaWI | WCCGGW | 2 cut(s) | 202, 893 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 209, 400, 536, 609, 713, 790 |
| Bse118I | RCCGGY | 1 cut(s) | 355 |
| Bse1I | ACTGG | 3 cut(s) | 75, 420, 644 |
| BseBI | CCWGG | 1 cut(s) | 548 |
| BseDI | CCNNGG | 3 cut(s) | 156, 423, 603 |
| BseGI | GGATG | 1 cut(s) | 799 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 209, 400, 536, 609, 713, 790 |
| BseNI | ACTGG | 3 cut(s) | 75, 420, 644 |
| BseRI | GAGGAG | 2 cut(s) | 270, 552 |
| BseSI | GKGCMC | 3 cut(s) | 165, 302, 706 |
| BseXI | GCAGC | 2 cut(s) | 157, 454 |
| BsgI | GTGCAG | 2 cut(s) | 127, 255 |
| Bsh1236I | CGCG | 1 cut(s) | 117 |
| BshFI | GGCC | 3 cut(s) | 63, 492, 721 |
| BshNI | GGYRCC | 2 cut(s) | 32, 703 |
| BsiHKAI | GWGCWC | 2 cut(s) | 165, 302 |
| BsiSI | CCGG | 4 cut(s) | 60, 203, 356, 894 |
| BslFI | GGGAC | 2 cut(s) | 256, 878 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 209, 400, 536, 609, 713, 790 |
| BsmAI | GTCTC | 1 cut(s) | 355 |
| BsmFI | GGGAC | 2 cut(s) | 256, 878 |
| BsnI | GGCC | 3 cut(s) | 63, 492, 721 |
| Bsp1286I | GDGCHC | 3 cut(s) | 165, 302, 706 |
| Bsp143I | GATC | 3 cut(s) | 418, 619, 679 |
| BspACI | CCGC | 2 cut(s) | 315, 600 |
| BspANI | GGCC | 3 cut(s) | 63, 492, 721 |
| BspFNI | CGCG | 1 cut(s) | 117 |
| BspLI | GGNNCC | 7 cut(s) | 34, 82, 94, 198, 420, 641, 705 |
| BspMI | ACCTGC | 1 cut(s) | 350 |
| BspPI | GGATC | 2 cut(s) | 413, 426 |
| BspT107I | GGYRCC | 2 cut(s) | 32, 703 |
| BsrFI | RCCGGY | 1 cut(s) | 355 |
| BsrI | ACTGG | 3 cut(s) | 75, 420, 644 |
| BssAI | RCCGGY | 1 cut(s) | 355 |
| BssECI | CCNNGG | 3 cut(s) | 156, 423, 603 |
| BssMI | GATC | 3 cut(s) | 418, 619, 679 |
| BssSI | CACGAG | 1 cut(s) | 164 |
| BssT1I | CCWWGG | 3 cut(s) | 156, 423, 603 |
| Bst2BI | CACGAG | 1 cut(s) | 164 |
| Bst2UI | CCWGG | 1 cut(s) | 548 |
| Bst4CI | ACNGT | 2 cut(s) | 487, 915 |
| Bst6I | CTCTTC | 2 cut(s) | 288, 945 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 39 |
| BstF5I | GGATG | 1 cut(s) | 799 |
| BstFNI | CGCG | 1 cut(s) | 117 |
| BstH2I | RGCGCY | 1 cut(s) | 23 |
| BstHHI | GCGC | 1 cut(s) | 22 |
| BstKTI | GATC | 3 cut(s) | 421, 622, 682 |
| BstMAI | GTCTC | 1 cut(s) | 355 |
| BstMBI | GATC | 3 cut(s) | 418, 619, 679 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 39, 275, 312 |
| BstNI | CCWGG | 1 cut(s) | 548 |
| BstSCI | CCNGG | 1 cut(s) | 546 |
| BstSFI | CTRYAG | 1 cut(s) | 483 |
| BstSLI | GKGCMC | 3 cut(s) | 165, 302, 706 |
| BstUI | CGCG | 1 cut(s) | 117 |
| BstV1I | GCAGC | 2 cut(s) | 157, 454 |
| BstX2I | RGATCY | 1 cut(s) | 418 |
| BstYI | RGATCY | 1 cut(s) | 418 |
| BsuRI | GGCC | 3 cut(s) | 63, 492, 721 |
| BtgZI | GCGATG | 1 cut(s) | 228 |
| BtsCI | GGATG | 1 cut(s) | 799 |
| BtsI | GCAGTG | 1 cut(s) | 225 |
| BtsIMutI | CAGTG | 2 cut(s) | 82, 225 |
| BveI | ACCTGC | 1 cut(s) | 350 |
| CfoI | GCGC | 1 cut(s) | 22 |
| Cfr10I | RCCGGY | 1 cut(s) | 355 |
| Csp6I | GTAC | 1 cut(s) | 911 |
| CviAII | CATG | 4 cut(s) | 494, 699, 868, 947 |
| CviQI | GTAC | 1 cut(s) | 911 |
| DpnI | GATC | 3 cut(s) | 420, 621, 681 |
| DpnII | GATC | 3 cut(s) | 418, 619, 679 |
| DraI | TTTAAA | 1 cut(s) | 366 |
| EaeI | YGGCCR | 2 cut(s) | 61, 490 |
| Eam1104I | CTCTTC | 2 cut(s) | 288, 945 |
| EarI | CTCTTC | 2 cut(s) | 288, 945 |
| EciI | GGCGGA | 1 cut(s) | 589 |
| Eco130I | CCWWGG | 3 cut(s) | 156, 423, 603 |
| Eco57I | CTGAAG | 3 cut(s) | 291, 431, 452 |
| EcoRI | GAATTC | 1 cut(s) | 206 |
| EcoRII | CCWGG | 1 cut(s) | 546 |
| EcoT14I | CCWWGG | 3 cut(s) | 156, 423, 603 |
| ErhI | CCWWGG | 3 cut(s) | 156, 423, 603 |
| FaeI | CATG | 4 cut(s) | 497, 702, 871, 950 |
| FaqI | GGGAC | 2 cut(s) | 256, 878 |
| FatI | CATG | 4 cut(s) | 493, 698, 867, 946 |
| FauNDI | CATATG | 2 cut(s) | 500, 724 |
| FbaI | TGATCA | 2 cut(s) | 619, 679 |
| FblI | GTMKAC | 2 cut(s) | 87, 482 |
| Fnu4HI | GCNGC | 2 cut(s) | 171, 443 |
| FokI | GGATG | 1 cut(s) | 806 |
| Fsp4HI | GCNGC | 2 cut(s) | 171, 443 |
| FspBI | CTAG | 2 cut(s) | 332, 927 |
| GlaI | GCGC | 1 cut(s) | 21 |
| GluI | GCNGC | 2 cut(s) | 171, 443 |
| HaeII | RGCGCY | 1 cut(s) | 23 |
| HaeIII | GGCC | 3 cut(s) | 63, 492, 721 |
| HapII | CCGG | 4 cut(s) | 60, 203, 356, 894 |
| HhaI | GCGC | 1 cut(s) | 22 |
| Hin1II | CATG | 4 cut(s) | 497, 702, 871, 950 |
| Hin6I | GCGC | 1 cut(s) | 20 |
| HinP1I | GCGC | 1 cut(s) | 20 |
| HincII | GTYRAC | 2 cut(s) | 88, 670 |
| HindII | GTYRAC | 2 cut(s) | 88, 670 |
| HindIII | AAGCTT | 1 cut(s) | 304 |
| HinfI | GANTC | 6 cut(s) | 110, 119, 348, 473, 526, 551 |
| HpaII | CCGG | 4 cut(s) | 60, 203, 356, 894 |
| HphI | GGTGA | 3 cut(s) | 345, 689, 742 |
| Hpy166II | GTNNAC | 5 cut(s) | 88, 163, 300, 483, 670 |
| Hpy188I | TCNGA | 2 cut(s) | 388, 462 |
| Hpy8I | GTNNAC | 5 cut(s) | 88, 163, 300, 483, 670 |
| Hpy99I | CGWCG | 3 cut(s) | 49, 89, 92 |
| HpyAV | CCTTC | 3 cut(s) | 145, 711, 792 |
| HpyCH4III | ACNGT | 2 cut(s) | 487, 915 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 39, 275, 312 |
| Hsp92II | CATG | 4 cut(s) | 497, 702, 871, 950 |
| HspAI | GCGC | 1 cut(s) | 20 |
| Ksp22I | TGATCA | 2 cut(s) | 619, 679 |
| Kzo9I | GATC | 3 cut(s) | 418, 619, 679 |
| LmnI | GCTCC | 2 cut(s) | 283, 645 |
| Lsp1109I | GCAGC | 2 cut(s) | 157, 454 |
| LweI | GCATC | 5 cut(s) | 205, 265, 379, 521, 778 |
| MaeI | CTAG | 2 cut(s) | 332, 927 |
| MaeIII | GTNAC | 2 cut(s) | 373, 833 |
| MalI | GATC | 3 cut(s) | 420, 621, 681 |
| MboI | GATC | 3 cut(s) | 418, 619, 679 |
| MboII | GAAGA | 7 cut(s) | 97, 120, 275, 284, 445, 826, 962 |
| MflI | RGATCY | 1 cut(s) | 418 |
| MhlI | GDGCHC | 3 cut(s) | 165, 302, 706 |
| MlsI | TGGCCA | 1 cut(s) | 492 |
| MluCI | AATT | 9 cut(s) | 177, 186, 206, 341, 436, 455, 574, 595, 614 |
| MluNI | TGGCCA | 1 cut(s) | 492 |
| MlyI | GAGTC | 3 cut(s) | 104, 113, 357 |
| MnlI | CCTC | 9 cut(s) | 107, 157, 160, 253, 291, 530, 721, 838, 946 |
| Mox20I | TGGCCA | 1 cut(s) | 492 |
| MscI | TGGCCA | 1 cut(s) | 492 |
| MseI | TTAA | 4 cut(s) | 309, 365, 594, 779 |
| Msp20I | TGGCCA | 1 cut(s) | 492 |
| MspI | CCGG | 4 cut(s) | 60, 203, 356, 894 |
| MspR9I | CCNGG | 1 cut(s) | 548 |
| MvaI | CCWGG | 1 cut(s) | 548 |
| MvnI | CGCG | 1 cut(s) | 117 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 39, 275, 312 |
| NdeI | CATATG | 2 cut(s) | 500, 724 |
| NdeII | GATC | 3 cut(s) | 418, 619, 679 |
| NlaIII | CATG | 4 cut(s) | 497, 702, 871, 950 |
| NlaIV | GGNNCC | 7 cut(s) | 34, 82, 94, 198, 420, 641, 705 |
| NmuCI | GTSAC | 1 cut(s) | 833 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 105, 114 |
| PfeI | GAWTC | 3 cut(s) | 473, 526, 551 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 609 |
| PkrI | GCNGC | 2 cut(s) | 172, 444 |
| PleI | GAGTC | 3 cut(s) | 104, 113, 356 |
| PpsI | GAGTC | 3 cut(s) | 104, 113, 356 |
| Psp6I | CCWGG | 1 cut(s) | 546 |
| PspGI | CCWGG | 1 cut(s) | 546 |
| PspN4I | GGNNCC | 7 cut(s) | 34, 82, 94, 198, 420, 641, 705 |
| PsuI | RGATCY | 1 cut(s) | 418 |
| RsaI | GTAC | 1 cut(s) | 912 |
| RsaNI | GTAC | 1 cut(s) | 911 |
| SalI | GTCGAC | 1 cut(s) | 86 |
| SaqAI | TTAA | 4 cut(s) | 309, 365, 594, 779 |
| SatI | GCNGC | 2 cut(s) | 171, 443 |
| Sau3AI | GATC | 3 cut(s) | 418, 619, 679 |
| SchI | GAGTC | 3 cut(s) | 104, 113, 357 |
| ScrFI | CCNGG | 1 cut(s) | 548 |
| SduI | GDGCHC | 3 cut(s) | 165, 302, 706 |
| SfaNI | GCATC | 5 cut(s) | 205, 265, 379, 521, 778 |
| SfcI | CTRYAG | 1 cut(s) | 483 |
| SgrDI | CGTCGACG | 1 cut(s) | 86 |
| Sse9I | AATT | 9 cut(s) | 177, 186, 206, 341, 436, 455, 574, 595, 614 |
| SsiI | CCGC | 2 cut(s) | 315, 600 |
| SspMI | CTAG | 2 cut(s) | 332, 927 |
| StyD4I | CCNGG | 1 cut(s) | 546 |
| StyI | CCWWGG | 3 cut(s) | 156, 423, 603 |
| TaaI | ACNGT | 2 cut(s) | 487, 915 |
| TaqI | TCGA | 2 cut(s) | 87, 108 |
| TasI | AATT | 9 cut(s) | 177, 186, 206, 341, 436, 455, 574, 595, 614 |
| TfiI | GAWTC | 3 cut(s) | 473, 526, 551 |
| Tru1I | TTAA | 4 cut(s) | 309, 365, 594, 779 |
| Tru9I | TTAA | 4 cut(s) | 309, 365, 594, 779 |
| TscAI | CASTG | 2 cut(s) | 82, 225 |
| TseFI | GTSAC | 1 cut(s) | 833 |
| TseI | GCWGC | 2 cut(s) | 170, 442 |
| Tsp45I | GTSAC | 1 cut(s) | 833 |
| TspDTI | ATGAA | 1 cut(s) | 963 |
| TspGWI | ACGGA | 3 cut(s) | 73, 105, 951 |
| TspRI | CASTG | 2 cut(s) | 82, 225 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 609 |
| VneI | GTGCAC | 2 cut(s) | 161, 298 |
| XapI | RAATTY | 3 cut(s) | 206, 455, 614 |
| XmiI | GTMKAC | 2 cut(s) | 87, 482 |
| XspI | CTAG | 2 cut(s) | 332, 927 |