Belongs to the WD repeat SEC13 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 389 |
| AciI | CCGC | 2 cut(s) | 84, 507 |
| AcoI | YGGCCR | 1 cut(s) | 397 |
| AcsI | RAATTY | 3 cut(s) | 177, 362, 521 |
| AcuI | CTGAAG | 1 cut(s) | 43 |
| AfaI | GTAC | 1 cut(s) | 819 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 443, 697 |
| AgsI | TTSAA | 5 cut(s) | 230, 248, 348, 481, 486 |
| AjnI | CCWGG | 1 cut(s) | 453 |
| AleI | CACNNNNGTG | 1 cut(s) | 747 |
| AluBI | AGCT | 3 cut(s) | 74, 256, 267 |
| AluI | AGCT | 3 cut(s) | 74, 256, 267 |
| Alw21I | GWGCWC | 1 cut(s) | 165 |
| Alw44I | GTGCAC | 1 cut(s) | 161 |
| AoxI | GGCC | 2 cut(s) | 397, 626 |
| ApaLI | GTGCAC | 1 cut(s) | 161 |
| ApoI | RAATTY | 3 cut(s) | 177, 362, 521 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 80, 112, 738, 770 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 56 |
| AsuHPI | GGTGA | 4 cut(s) | 306, 527, 596, 761 |
| BaeGI | GKGCMC | 2 cut(s) | 165, 613 |
| BalI | TGGCCA | 1 cut(s) | 399 |
| BauI | CACGAG | 2 cut(s) | 164, 354 |
| Bbv12I | GWGCWC | 1 cut(s) | 165 |
| BccI | CCATC | 6 cut(s) | 44, 110, 191, 537, 695, 803 |
| BciT130I | CCWGG | 1 cut(s) | 455 |
| BclI | TGATCA | 2 cut(s) | 526, 586 |
| BfaI | CTAG | 3 cut(s) | 75, 293, 573 |
| BfmI | CTRYAG | 1 cut(s) | 390 |
| Bme1390I | CCNGG | 1 cut(s) | 455 |
| BmiI | GGNNCC | 2 cut(s) | 82, 548 |
| BmrFI | CCNGG | 1 cut(s) | 455 |
| BmrI | ACTGGG | 1 cut(s) | 545 |
| BmsI | GCATC | 3 cut(s) | 223, 428, 685 |
| BmuI | ACTGGG | 1 cut(s) | 545 |
| BsaJI | CCNNGG | 2 cut(s) | 156, 510 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 443, 697 |
| Bse118I | RCCGGY | 1 cut(s) | 316 |
| Bse1I | ACTGG | 1 cut(s) | 551 |
| BseBI | CCWGG | 1 cut(s) | 455 |
| BseDI | CCNNGG | 2 cut(s) | 156, 510 |
| BseGI | GGATG | 2 cut(s) | 131, 706 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 443, 697 |
| BseNI | ACTGG | 1 cut(s) | 551 |
| BseRI | GAGGAG | 1 cut(s) | 459 |
| BseSI | GKGCMC | 2 cut(s) | 165, 613 |
| BsgI | GTGCAG | 2 cut(s) | 127, 213 |
| BshFI | GGCC | 2 cut(s) | 399, 628 |
| BsiHKAI | GWGCWC | 1 cut(s) | 165 |
| BsiSI | CCGG | 1 cut(s) | 317 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 443, 697 |
| BsnI | GGCC | 2 cut(s) | 399, 628 |
| Bsp1286I | GDGCHC | 2 cut(s) | 165, 613 |
| Bsp143I | GATC | 2 cut(s) | 526, 586 |
| BspACI | CCGC | 2 cut(s) | 84, 507 |
| BspANI | GGCC | 2 cut(s) | 399, 628 |
| BspLI | GGNNCC | 2 cut(s) | 82, 548 |
| BsrFI | RCCGGY | 1 cut(s) | 316 |
| BsrI | ACTGG | 1 cut(s) | 551 |
| BssAI | RCCGGY | 1 cut(s) | 316 |
| BssECI | CCNNGG | 2 cut(s) | 156, 510 |
| BssMI | GATC | 2 cut(s) | 526, 586 |
| BssSI | CACGAG | 2 cut(s) | 164, 354 |
| BssT1I | CCWWGG | 2 cut(s) | 156, 510 |
| Bst2BI | CACGAG | 2 cut(s) | 164, 354 |
| Bst2UI | CCWGG | 1 cut(s) | 455 |
| Bst4CI | ACNGT | 2 cut(s) | 394, 822 |
| Bst6I | CTCTTC | 1 cut(s) | 249 |
| BstF5I | GGATG | 2 cut(s) | 131, 706 |
| BstKTI | GATC | 2 cut(s) | 529, 589 |
| BstMBI | GATC | 2 cut(s) | 526, 586 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 563 |
| BstNI | CCWGG | 1 cut(s) | 455 |
| BstSCI | CCNGG | 1 cut(s) | 453 |
| BstSFI | CTRYAG | 1 cut(s) | 390 |
| BstSLI | GKGCMC | 2 cut(s) | 165, 613 |
| BsuRI | GGCC | 2 cut(s) | 399, 628 |
| BtsCI | GGATG | 2 cut(s) | 131, 706 |
| BtsIMutI | CAGTG | 1 cut(s) | 611 |
| Cfr10I | RCCGGY | 1 cut(s) | 316 |
| Csp6I | GTAC | 1 cut(s) | 818 |
| CviAII | CATG | 2 cut(s) | 401, 775 |
| CviQI | GTAC | 1 cut(s) | 818 |
| DpnI | GATC | 2 cut(s) | 528, 588 |
| DpnII | GATC | 2 cut(s) | 526, 586 |
| DraI | TTTAAA | 1 cut(s) | 327 |
| EaeI | YGGCCR | 1 cut(s) | 397 |
| Eam1104I | CTCTTC | 1 cut(s) | 249 |
| EarI | CTCTTC | 1 cut(s) | 249 |
| EciI | GGCGGA | 2 cut(s) | 73, 496 |
| Eco130I | CCWWGG | 2 cut(s) | 156, 510 |
| Eco57I | CTGAAG | 1 cut(s) | 43 |
| EcoRII | CCWGG | 1 cut(s) | 453 |
| EcoT14I | CCWWGG | 2 cut(s) | 156, 510 |
| ErhI | CCWWGG | 2 cut(s) | 156, 510 |
| FaeI | CATG | 2 cut(s) | 404, 778 |
| FatI | CATG | 2 cut(s) | 400, 774 |
| FauNDI | CATATG | 2 cut(s) | 407, 631 |
| FbaI | TGATCA | 2 cut(s) | 526, 586 |
| FblI | GTMKAC | 1 cut(s) | 389 |
| FokI | GGATG | 2 cut(s) | 118, 713 |
| FspBI | CTAG | 3 cut(s) | 75, 293, 573 |
| HaeIII | GGCC | 2 cut(s) | 399, 628 |
| HapII | CCGG | 1 cut(s) | 317 |
| Hin1II | CATG | 2 cut(s) | 404, 778 |
| HindIII | AAGCTT | 1 cut(s) | 265 |
| HinfI | GANTC | 5 cut(s) | 110, 119, 380, 433, 458 |
| HpaII | CCGG | 1 cut(s) | 317 |
| HphI | GGTGA | 4 cut(s) | 306, 527, 596, 761 |
| Hpy166II | GTNNAC | 2 cut(s) | 163, 390 |
| Hpy188I | TCNGA | 2 cut(s) | 369, 520 |
| Hpy8I | GTNNAC | 2 cut(s) | 163, 390 |
| Hpy99I | CGWCG | 1 cut(s) | 49 |
| HpyAV | CCTTC | 4 cut(s) | 106, 145, 618, 699 |
| HpyCH4III | ACNGT | 2 cut(s) | 394, 822 |
| HpyCH4IV | ACGT | 1 cut(s) | 788 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 563 |
| HpySE526I | ACGT | 1 cut(s) | 788 |
| Hsp92II | CATG | 2 cut(s) | 404, 778 |
| Ksp22I | TGATCA | 2 cut(s) | 526, 586 |
| Kzo9I | GATC | 2 cut(s) | 526, 586 |
| LmnI | GCTCC | 1 cut(s) | 552 |
| LweI | GCATC | 3 cut(s) | 223, 428, 685 |
| MaeI | CTAG | 3 cut(s) | 75, 293, 573 |
| MaeII | ACGT | 1 cut(s) | 788 |
| MaeIII | GTNAC | 2 cut(s) | 575, 740 |
| MalI | GATC | 2 cut(s) | 528, 588 |
| MboI | GATC | 2 cut(s) | 526, 586 |
| MboII | GAAGA | 4 cut(s) | 97, 130, 236, 242 |
| MhlI | GDGCHC | 2 cut(s) | 165, 613 |
| MlsI | TGGCCA | 1 cut(s) | 399 |
| MluNI | TGGCCA | 1 cut(s) | 399 |
| MlyI | GAGTC | 2 cut(s) | 104, 113 |
| MmeI | TCCRAC | 1 cut(s) | 154 |
| MnlI | CCTC | 7 cut(s) | 157, 160, 211, 252, 437, 628, 745 |
| Mox20I | TGGCCA | 1 cut(s) | 399 |
| MroXI | GAANNNNTTC | 1 cut(s) | 56 |
| MscI | TGGCCA | 1 cut(s) | 399 |
| MseI | TTAA | 4 cut(s) | 270, 326, 501, 686 |
| MslI | CAYNNNNRTG | 1 cut(s) | 747 |
| Msp20I | TGGCCA | 1 cut(s) | 399 |
| MspI | CCGG | 1 cut(s) | 317 |
| MspR9I | CCNGG | 1 cut(s) | 455 |
| MvaI | CCWGG | 1 cut(s) | 455 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 563 |
| NdeI | CATATG | 2 cut(s) | 407, 631 |
| NdeII | GATC | 2 cut(s) | 526, 586 |
| NlaIII | CATG | 2 cut(s) | 404, 778 |
| NlaIV | GGNNCC | 2 cut(s) | 82, 548 |
| NmuCI | GTSAC | 2 cut(s) | 575, 740 |
| OliI | CACNNNNGTG | 1 cut(s) | 747 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 105 |
| PdmI | GAANNNNTTC | 1 cut(s) | 56 |
| PfeI | GAWTC | 3 cut(s) | 380, 433, 458 |
| PleI | GAGTC | 2 cut(s) | 104, 113 |
| PpsI | GAGTC | 2 cut(s) | 104, 113 |
| Psp6I | CCWGG | 1 cut(s) | 453 |
| PspGI | CCWGG | 1 cut(s) | 453 |
| PspN4I | GGNNCC | 2 cut(s) | 82, 548 |
| RsaI | GTAC | 1 cut(s) | 819 |
| RsaNI | GTAC | 1 cut(s) | 818 |
| RseI | CAYNNNNRTG | 1 cut(s) | 747 |
| SaqAI | TTAA | 4 cut(s) | 270, 326, 501, 686 |
| Sau3AI | GATC | 2 cut(s) | 526, 586 |
| SchI | GAGTC | 2 cut(s) | 104, 113 |
| ScrFI | CCNGG | 1 cut(s) | 455 |
| SduI | GDGCHC | 2 cut(s) | 165, 613 |
| SfaNI | GCATC | 3 cut(s) | 223, 428, 685 |
| SfcI | CTRYAG | 1 cut(s) | 390 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 747 |
| SsiI | CCGC | 2 cut(s) | 84, 507 |
| SspMI | CTAG | 3 cut(s) | 75, 293, 573 |
| StyD4I | CCNGG | 1 cut(s) | 453 |
| StyI | CCWWGG | 2 cut(s) | 156, 510 |
| TaaI | ACNGT | 2 cut(s) | 394, 822 |
| TaiI | ACGT | 1 cut(s) | 791 |
| TaqI | TCGA | 1 cut(s) | 108 |
| TfiI | GAWTC | 3 cut(s) | 380, 433, 458 |
| Tru1I | TTAA | 4 cut(s) | 270, 326, 501, 686 |
| Tru9I | TTAA | 4 cut(s) | 270, 326, 501, 686 |
| TscAI | CASTG | 1 cut(s) | 618 |
| TseFI | GTSAC | 2 cut(s) | 575, 740 |
| Tsp45I | GTSAC | 2 cut(s) | 575, 740 |
| TspDTI | ATGAA | 1 cut(s) | 120 |
| TspRI | CASTG | 1 cut(s) | 618 |
| VneI | GTGCAC | 1 cut(s) | 161 |
| XapI | RAATTY | 3 cut(s) | 177, 362, 521 |
| XmiI | GTMKAC | 1 cut(s) | 389 |
| XmnI | GAANNNNTTC | 1 cut(s) | 56 |
| XspI | CTAG | 3 cut(s) | 75, 293, 573 |