MD06G1107400.v1.1

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001166
Physical Location & Seq
Forward (+)
24738773 .. 24741280
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1107400.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGATGATCGTCTTGGAAATATAAAGATCATAGGAGATCATTTTAAAGCATCCGAACCCAATATCGACTCTTCTCAGGACATAGCTTCATCTATCTCGCGACCAAGAACTGATAATTATCCAAGGTCTCGCTTGTGGAGTAGGAGAAAATTAAGAAGTGCAGCATACATGTTGAAATTGAACTTCTTTAGTCTCCGCGGACTGCCATGGGGGTCTAGCACAGATGGCGAAGAAAAGGTTGAGCTAACTGCTGTAGAACTAGAATCACTTCGATCGGAGCTTGCTGATTTAGAACAGAAGGAAGCGCACTTGAAAGCTCAATTGGAACACGTTGATGAAGTTTTGCGATCTGCTCGTCTCGCTGGCTATTTGTACATCCGAACTAGGTGGAAACCACTACCAGGAGAACCTCCCCCTCTTGATGATACTGACATTGATGACTGGCTTCCTCGCTTTGTTGTCTTGCAAGGGCCGTGCATTTTCTTCTTCTTGTTTTCCACAGATTTGAGTCCTCAAGACTCCACCCTATTAGCTGATATTGTTGAAATAGGCCCCTTGCCAAGCATTACACGAGACGATGAAGGAACTCGACATGCATTTCGTATATTAACTCGTCAAGGACTGCGATTCGAGTGCTCAAGCGCTTCTAAGGAACAGGTGGACTCGTGGTTGTCAGCATTACGAACGGACTGCAAATTCGAGGGTTCAGATGCCGCCACAGATCCAAACAACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00983 ko01100 ko01110 ko01130 ko04016 map00230 map00240 map00983 map01100 map01110 map01130 map04016
Pfam Domains
Protein Families
PH

Protein Analysis

246

Amino Acids

27.77

Weight (kDa)

5.06

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 100, 198
AciI CCGC 3 cut(s) 196, 198, 714
AclWI GGATC 1 cut(s) 716
AcsI RAATTY 1 cut(s) 695
AfaI GTAC 1 cut(s) 374
AfeI AGCGCT 1 cut(s) 643
AfiI CCNNNNNNNGG 1 cut(s) 401
AflIII ACRYGT 2 cut(s) 168, 328
AgsI TTSAA 4 cut(s) 175, 181, 313, 545
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 5 cut(s) 86, 244, 280, 317, 533
AluI AGCT 5 cut(s) 86, 244, 280, 317, 533
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 4 cut(s) 132, 197, 362, 567
AlwI GGATC 1 cut(s) 716
Aor51HI AGCGCT 1 cut(s) 643
AoxI GGCC 2 cut(s) 470, 550
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 695
ArsI GACNNNNNNTTYG 4 cut(s) 582, 614, 680, 712
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 307, 644
AspS9I GGNCC 2 cut(s) 470, 551
BauI CACGAG 2 cut(s) 570, 664
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 173
BccI CCATC 1 cut(s) 218
BceAI ACGGC 1 cut(s) 457
BcgI CGANNNNNNTGC 4 cut(s) 615, 649, 672, 706
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 4 cut(s) 132, 197, 362, 567
BfaI CTAG 3 cut(s) 216, 260, 384
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 645
BisI GCNGC 2 cut(s) 162, 714
BlsI GCNGC 2 cut(s) 163, 715
Bme1390I CCNGG 1 cut(s) 402
BmgT120I GGNCC 2 cut(s) 470, 551
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 59, 700
BpuEI CTTGAG 2 cut(s) 498, 622
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 122, 196, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 446
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 122, 196, 206
BseGI GGATG 3 cut(s) 10, 50, 375
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 446
BseXI GCAGC 1 cut(s) 173
BsgI GTGCAG 1 cut(s) 180
Bsh1236I CGCG 2 cut(s) 100, 198
Bsh1285I CGRYCG 1 cut(s) 275
BshFI GGCC 2 cut(s) 472, 552
BsiEI CGRYCG 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 638
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 4 cut(s) 132, 197, 362, 567
BsmBI CGTCTC 2 cut(s) 362, 567
BsnI GGCC 2 cut(s) 472, 552
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 638
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 6 cut(s) 7, 27, 37, 272, 347, 721
Bsp19I CCATGG 1 cut(s) 206
Bsp68I TCGCGA 1 cut(s) 100
BspACI CCGC 3 cut(s) 196, 198, 714
BspANI GGCC 2 cut(s) 472, 552
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 2 cut(s) 100, 198
BspLI GGNNCC 1 cut(s) 553
BspPI GGATC 1 cut(s) 716
BspTNI GGTCTC 1 cut(s) 132
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 122, 196, 206
BssMI GATC 6 cut(s) 7, 27, 37, 272, 347, 721
BssSI CACGAG 2 cut(s) 570, 664
BssT1I CCWWGG 2 cut(s) 122, 206
Bst2BI CACGAG 2 cut(s) 570, 664
Bst2UI CCWGG 1 cut(s) 402
Bst6I CTCTTC 1 cut(s) 76
BstAUI TGTACA 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 282, 364
BstDEI CTNAG 2 cut(s) 75, 648
BstDSI CCRYGG 2 cut(s) 196, 206
BstF5I GGATG 3 cut(s) 10, 50, 375
BstFNI CGCG 2 cut(s) 100, 198
BstH2I RGCGCY 1 cut(s) 645
BstHHI GCGC 2 cut(s) 307, 644
BstKTI GATC 6 cut(s) 10, 30, 40, 275, 350, 724
BstMAI GTCTC 4 cut(s) 132, 197, 362, 567
BstMBI GATC 6 cut(s) 7, 27, 37, 272, 347, 721
BstMCI CGRYCG 1 cut(s) 275
BstMWI GCNNNNNNNGC 2 cut(s) 225, 359
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 2 cut(s) 172, 596
BstSCI CCNGG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 252
BstUI CGCG 2 cut(s) 100, 198
BstV1I GCAGC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
BsuRI GGCC 2 cut(s) 472, 552
BtgI CCRYGG 2 cut(s) 196, 206
BtsCI GGATG 3 cut(s) 10, 50, 375
BtuMI TCGCGA 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 282, 364
CfoI GCGC 2 cut(s) 307, 644
Cfr13I GGNCC 2 cut(s) 470, 551
Cfr42I CCGCGG 1 cut(s) 199
Csp6I GTAC 1 cut(s) 373
CviAII CATG 3 cut(s) 169, 207, 593
CviJI RGCY 9 cut(s) 86, 244, 280, 317, 366, 445, 472, 533, 552
CviKI_1 RGCY 9 cut(s) 86, 244, 280, 317, 366, 445, 472, 533, 552
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 2 cut(s) 75, 648
DpnI GATC 6 cut(s) 9, 29, 39, 274, 349, 723
DpnII GATC 6 cut(s) 7, 27, 37, 272, 347, 721
DraI TTTAAA 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Eco130I CCWWGG 2 cut(s) 122, 206
Eco31I GGTCTC 1 cut(s) 132
Eco47III AGCGCT 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 122, 206
EcoT22I ATGCAT 1 cut(s) 598
ErhI CCWWGG 2 cut(s) 122, 206
Esp3I CGTCTC 2 cut(s) 362, 567
FaeI CATG 3 cut(s) 172, 210, 596
FaiI YATR 8 cut(s) 23, 32, 83, 166, 170, 208, 594, 605
FatI CATG 3 cut(s) 168, 206, 592
Fnu4HI GCNGC 2 cut(s) 162, 714
FokI GGATG 3 cut(s) 17, 37, 362
Fsp4HI GCNGC 2 cut(s) 162, 714
FspBI CTAG 3 cut(s) 216, 260, 384
GlaI GCGC 2 cut(s) 306, 643
GluI GCNGC 2 cut(s) 162, 714
HaeII RGCGCY 1 cut(s) 645
HaeIII GGCC 2 cut(s) 472, 552
HhaI GCGC 2 cut(s) 307, 644
Hin1II CATG 3 cut(s) 172, 210, 596
Hin6I GCGC 2 cut(s) 305, 642
HinP1I GCGC 2 cut(s) 305, 642
HinfI GANTC 6 cut(s) 68, 263, 508, 518, 627, 662
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 4 cut(s) 55, 277, 380, 709
Hpy188III TCNNGA 4 cut(s) 77, 99, 419, 515
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 2 cut(s) 292, 575
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 5 cut(s) 161, 466, 477, 596, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 359
HpyF3I CTNAG 2 cut(s) 75, 648
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 172, 210, 596
HspAI GCGC 2 cut(s) 305, 642
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 6 cut(s) 7, 27, 37, 272, 347, 721
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 6 cut(s) 62, 348, 387, 414, 427, 641
Lsp1109I GCAGC 1 cut(s) 173
LweI GCATC 2 cut(s) 59, 700
MaeI CTAG 3 cut(s) 216, 260, 384
MaeII ACGT 1 cut(s) 330
MalI GATC 6 cut(s) 9, 29, 39, 274, 349, 723
MboI GATC 6 cut(s) 7, 27, 37, 272, 347, 721
MboII GAAGA 4 cut(s) 63, 242, 475, 478
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 721
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 5 cut(s) 115, 149, 176, 320, 695
MlyI GAGTC 4 cut(s) 62, 512, 517, 656
MnlI CCTC 5 cut(s) 420, 426, 459, 522, 694
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 3 cut(s) 45, 152, 608
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 402
MunI CAATTG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 100, 198
MwoI GCNNNNNNNGC 2 cut(s) 225, 359
NcoI CCATGG 1 cut(s) 206
NdeII GATC 6 cut(s) 7, 27, 37, 272, 347, 721
NlaIII CATG 3 cut(s) 172, 210, 596
NlaIV GGNNCC 1 cut(s) 553
NruI TCGCGA 1 cut(s) 100
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 2 cut(s) 172, 596
PciI ACATGT 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 2 cut(s) 263, 627
PkrI GCNGC 2 cut(s) 163, 715
Ple19I CGATCG 1 cut(s) 275
PleI GAGTC 4 cut(s) 62, 512, 516, 656
PpsI GAGTC 4 cut(s) 62, 512, 516, 656
PscI ACATGT 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 2 cut(s) 470, 551
PsuI RGATCY 1 cut(s) 721
PvuI CGATCG 1 cut(s) 275
RruI TCGCGA 1 cut(s) 100
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 333
SacII CCGCGG 1 cut(s) 199
SaqAI TTAA 3 cut(s) 45, 152, 608
SatI GCNGC 2 cut(s) 162, 714
Sau3AI GATC 6 cut(s) 7, 27, 37, 272, 347, 721
Sau96I GGNCC 2 cut(s) 470, 551
SchI GAGTC 4 cut(s) 62, 512, 517, 656
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 638
SfaNI GCATC 2 cut(s) 59, 700
SfcI CTRYAG 1 cut(s) 252
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 2 cut(s) 513, 637
SmoI CTYRAG 2 cut(s) 513, 637
Sse9I AATT 5 cut(s) 115, 149, 176, 320, 695
SsiI CCGC 3 cut(s) 196, 198, 714
SspMI CTAG 3 cut(s) 216, 260, 384
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 2 cut(s) 122, 206
TaiI ACGT 1 cut(s) 333
TaqI TCGA 5 cut(s) 66, 271, 589, 630, 699
TasI AATT 5 cut(s) 115, 149, 176, 320, 695
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 2 cut(s) 263, 627
Tru1I TTAA 3 cut(s) 45, 152, 608
Tru9I TTAA 3 cut(s) 45, 152, 608
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 78, 351, 594
TspGWI ACGGA 1 cut(s) 701
XapI RAATTY 1 cut(s) 695
XceI RCATGY 2 cut(s) 172, 596
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 3 cut(s) 216, 260, 384
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.