MD14G1129100.v1.1

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001166
Physical Location & Seq
Forward (+)
20658602 .. 20661466
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1129100.v1.1.491

Sequence Viewer

Length: 561 bp
ATGTTGAAATTCTTTAGTCTGCGGGGACTGCCATGGGGGTCTAGCACAGATGGGGAAGAAAAGGTTGAGCTAACTGCTGTAGAACTAGAATCACTTCGATCGGATCTTGCTGATTTAGAAGAGAAGGAAGCGCACTTGAAAGCTCAATTGGAACACGTTGATGAAATTTTGCGATCTGCTCGTCTCTCTGGCTATCTGTACATCCGAACTAGGTGGAAACCACTACCAGGGGAACCTCCCCCCCTTGATGATACTGACGTTGATGACTGGCTTCCTCGCTTTGTTGTTTTGCAAGGGCCGTGCATTTTCTTCTACCTGTTTTCCACGGATTTGAGTCCTCAAGACTCCACCCTATTAGCTGATATTGTTGAAGTAAGCCCCTTGCCCAGCTTTACACGAGACGATGAAGGAACTCGAACTGCGTTTTGTATCTTAACTCGTCAAGGACTGCGATTCGAGTGCTCAAGCGCTTCTAAGGCGCAGGTGGACTCTTGGTTGTCAGCGTTACGAACGGACTGCAAATTGGAGGGTTCAGACGCCACAGATCCAAAGGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00983 ko01100 ko01110 ko01130 ko04016 map00230 map00240 map00983 map01100 map01110 map01130 map04016
Pfam Domains
Protein Families
PH

Protein Analysis

187

Amino Acids

20.93

Weight (kDa)

4.5

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 62 - 171 8.9e-07 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AciI CCGC 1 cut(s) 22
AclWI GGATC 2 cut(s) 111, 539
AcsI RAATTY 2 cut(s) 8, 165
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 1 cut(s) 200
AfeI AGCGCT 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 3 cut(s) 7, 139, 371
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 4 cut(s) 70, 143, 359, 390
AluI AGCT 4 cut(s) 70, 143, 359, 390
Alw21I GWGCWC 1 cut(s) 464
Alw26I GTCTC 2 cut(s) 188, 393
AlwI GGATC 2 cut(s) 111, 539
Aor51HI AGCGCT 1 cut(s) 469
AoxI GGCC 1 cut(s) 296
ApoI RAATTY 2 cut(s) 8, 165
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 3 cut(s) 133, 470, 481
AspS9I GGNCC 1 cut(s) 296
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BauI CACGAG 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 464
BccI CCATC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 283
BcgI CGANNNNNNTGC 4 cut(s) 441, 475, 498, 532
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 188, 393
BfaI CTAG 3 cut(s) 42, 86, 210
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 472
Bme1390I CCNGG 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 324, 448
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 3 cut(s) 32, 227, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 32, 227, 324
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 227
BseNI ACTGG 1 cut(s) 272
BseYI CCCAGC 1 cut(s) 386
Bsh1285I CGRYCG 1 cut(s) 101
BshFI GGCC 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 464
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 227
BsmAI GTCTC 2 cut(s) 188, 393
BsmBI CGTCTC 2 cut(s) 188, 393
BsmFI GGGAC 1 cut(s) 39
BsnI GGCC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 464
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 4 cut(s) 98, 103, 173, 544
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 2 cut(s) 111, 539
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 32, 227, 324
BssMI GATC 4 cut(s) 98, 103, 173, 544
BssNI GRCGYC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 114
BstACI GRCGYC 1 cut(s) 537
BstAUI TGTACA 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 474
BstDSI CCRYGG 2 cut(s) 32, 324
BstF5I GGATG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 3 cut(s) 133, 470, 481
BstKTI GATC 4 cut(s) 101, 106, 176, 547
BstMAI GTCTC 2 cut(s) 188, 393
BstMBI GATC 4 cut(s) 98, 103, 173, 544
BstMCI CGRYCG 1 cut(s) 101
BstMWI GCNNNNNNNGC 2 cut(s) 28, 476
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 103, 544
BstYI RGATCY 2 cut(s) 103, 544
BsuRI GGCC 1 cut(s) 298
BtgI CCRYGG 2 cut(s) 32, 324
BtsCI GGATG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 472
CfoI GCGC 3 cut(s) 133, 470, 481
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 545
Csp6I GTAC 1 cut(s) 199
CviAII CATG 1 cut(s) 33
CviJI RGCY 8 cut(s) 70, 143, 192, 271, 298, 359, 378, 390
CviKI_1 RGCY 8 cut(s) 70, 143, 192, 271, 298, 359, 378, 390
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 1 cut(s) 474
DpnI GATC 4 cut(s) 100, 105, 175, 546
DpnII GATC 4 cut(s) 98, 103, 173, 544
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 32
Eco47III AGCGCT 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 32
Esp3I CGTCTC 2 cut(s) 188, 393
FaeI CATG 1 cut(s) 36
FaiI YATR 1 cut(s) 34
FaqI GGGAC 1 cut(s) 39
FatI CATG 1 cut(s) 32
FauI CCCGC 1 cut(s) 15
FokI GGATG 1 cut(s) 188
FspBI CTAG 3 cut(s) 42, 86, 210
GlaI GCGC 3 cut(s) 132, 469, 480
GsaI CCCAGC 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 1 cut(s) 298
HgaI GACGC 1 cut(s) 545
HhaI GCGC 3 cut(s) 133, 470, 481
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 1 cut(s) 36
Hin6I GCGC 3 cut(s) 131, 468, 479
HinP1I GCGC 3 cut(s) 131, 468, 479
HinfI GANTC 5 cut(s) 89, 334, 344, 453, 488
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 3 cut(s) 103, 206, 535
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 2 cut(s) 118, 401
HpyCH4IV ACGT 2 cut(s) 156, 258
HpyCH4V TGCA 3 cut(s) 292, 303, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 476
HpyF3I CTNAG 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 156, 258
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 1 cut(s) 36
HspAI GCGC 3 cut(s) 131, 468, 479
Kzo9I GATC 4 cut(s) 98, 103, 173, 544
LpnPI CCDG 7 cut(s) 174, 213, 240, 253, 329, 400, 467
MaeI CTAG 3 cut(s) 42, 86, 210
MaeII ACGT 2 cut(s) 156, 258
MaeIII GTNAC 1 cut(s) 504
MalI GATC 4 cut(s) 100, 105, 175, 546
MboI GATC 4 cut(s) 98, 103, 173, 544
MboII GAAGA 3 cut(s) 68, 131, 301
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 2 cut(s) 103, 544
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 4 cut(s) 8, 146, 165, 521
MlyI GAGTC 3 cut(s) 338, 343, 482
MnlI CCTC 4 cut(s) 246, 285, 348, 520
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 28, 476
NcoI CCATGG 1 cut(s) 32
NdeII GATC 4 cut(s) 98, 103, 173, 544
NlaIII CATG 1 cut(s) 36
NlaIV GGNNCC 1 cut(s) 234
PaqCI CACCTGC 1 cut(s) 472
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 2 cut(s) 89, 453
Ple19I CGATCG 1 cut(s) 101
PleI GAGTC 3 cut(s) 338, 342, 482
PpsI GAGTC 3 cut(s) 338, 342, 482
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 296
PsuI RGATCY 2 cut(s) 103, 544
PvuI CGATCG 1 cut(s) 101
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 4 cut(s) 98, 103, 173, 544
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 3 cut(s) 338, 343, 482
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 339, 463
SmoI CTYRAG 2 cut(s) 339, 463
Sse9I AATT 4 cut(s) 8, 146, 165, 521
SsiI CCGC 1 cut(s) 22
SspMI CTAG 3 cut(s) 42, 86, 210
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 32
TaiI ACGT 2 cut(s) 159, 261
TaqI TCGA 3 cut(s) 97, 415, 456
TasI AATT 4 cut(s) 8, 146, 165, 521
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 2 cut(s) 89, 453
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TspDTI ATGAA 2 cut(s) 177, 420
TspGWI ACGGA 2 cut(s) 341, 527
XapI RAATTY 2 cut(s) 8, 165
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 3 cut(s) 42, 86, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.