Rh7AG145300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001166
Physical Location & Seq
Reverse (-)
11657258 .. 11661240
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG145300.1

Sequence Viewer

Length: 1719 bp
ATGATGAAGAGCTCTAGGACGATGTCGTCTCGGAAGCAGAGCAAGCACAGTTCAAGCAACTCGGACTCGGAGGTAGAAATAGTTGATGACGATGAAAGAGAAGGGAGGACAAGGAAGGCCCAGAAGTGTAAAAAGGAGAGTTCCGTCAGGGTTTCTTACACTGGCGAGAAGCGAAGATTGGGAGACAGGGGCAATAATCCGAGAAATAGAGCTGATGATAGAGAGGACCAAGCCCATAGCAGATCCAGAAGTACCAAGGAACAACATGTTGATCCTGAAAAGAATGATGGAGGGTACACTAGGTATAAGGATGATAGGGGAAATAGTGCTGATGATAGAGAGGACCAAAGCTATAGCAGATCCCGAAGTACCAAGGAACGACATGTTGATCCAGAAAAGAATGCTGGAGAATTCACTAGGTATAATGATGATAGGGGAACTAGAGCTGATGATAGAGAGGACCGAAGCTATAGTAGATTCCGAAGTACCAAGGAACAACATTTTGATCTAGAAAAGAAAGTCAGTGGCATGGCAGAGCCTATTGCTGATAAGGAAGGGTCTCGTTTACACCATGCTGATGCAGACTCAAATGCAAGCTGTAACAGACAGAGGAATTCACCTAGCTCTTATTCTCATCTTTCAAAAGATCAATCCAGTGCAAGACCTGTGATGGATATAAATCACTCTGCATTTTCTCATCATGTCCCTGATGCTGGCAGATACTTCAGCCATGGGCATCAATATGGGTGGCAAAATCCTGTTAATAATGGTTCATACCCACCATTGCCTGCGCGAGATGCAAATAATGTTGCCACCAGGTCTGAGGCTCCAGCATATGGGACGTTTGATTGGAACCACAATACAGAATCAATTGGCGACCAGCAGTTGGAGACAAGTGCATATATGTTGAAGGGCTTAACTAGTGGTGTGCATCCAGAGCTTCATTTAGTCTCTCAGGAAGAGAATCCAGCAGTATATGCAAAAGAAGATGAAACTTGCTTGGGGGTATCAAGGCTGCAAGAGAACCAGAGTGATACTAATTTGGACAAGGGTCAGAAGATTGAAATTGGTCTAAATAGTGATGATTTAGCAAAGGAACTATGTAAACCGTCCAAAAGTATTACTGAGAAATTACCTAAACTTTCTAAGATACCAAAGGGTGATGATGGTCATCTTTGGCTTATGTATCTCTCCAGGCTTGACATTTCAACAGAGCTTACTCATCCAAAGCTATACAATAAATGCCAGACTCTAAGAGCGATGTCCTTTTACAAAAAGCAGAGAGAAGAGATGAGGGCCATAACTCATCCCTTATTGATAGATGGACAACATACATCATTTTATAGTGCACAGAAACTGGATGTTGTTCCGATTTGTGACCGAGAGAATGACGTTGTGCCATTTTCATCTTTTAATGAGCTCAAACAAGGGCAGTTTTTCCCAGCTTGTTCTGCCCCCCACAACAAGGTAGATGAATATGCTCATTTACTCAGTGTGGAGAAATCATGTGAACCAAGTCCAAGGCAAGTCGATATAAGTATGATTTTGGATGTAGAGACTGTAAATGGCTGCATCAAGGATAAGTCTCCTACTCTCCACAATGCATCGCAATCTGCTGCCAGCGGGCCTTGTTATCTGAGTGGGCTTTGCGACTTCCATTTTGCTGGTAGCACTGAGGAAAAGAAAAGAAGTCACTTGATTTTAGGTCTGATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

572

Amino Acids

64.62

Weight (kDa)

6.5

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccB7I CCANNNNNTGG 2 cut(s) 836, 886
AccII CGCG 1 cut(s) 793
AciI CCGC 1 cut(s) 1623
AclWI GGATC 4 cut(s) 237, 266, 354, 383
AcsI RAATTY 2 cut(s) 410, 613
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 4 cut(s) 253, 296, 370, 487
AfiI CCNNNNNNNGG 4 cut(s) 713, 836, 886, 1465
AflIII ACRYGT 2 cut(s) 265, 382
AgsI TTSAA 5 cut(s) 54, 642, 910, 1064, 1209
AhlI ACTAGT 1 cut(s) 920
AjnI CCWGG 2 cut(s) 815, 1193
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
Alw21I GWGCWC 3 cut(s) 14, 1351, 1422
Alw26I GTCTC 7 cut(s) 33, 177, 564, 884, 955, 1550, 1590
Alw44I GTGCAC 1 cut(s) 1347
AlwI GGATC 4 cut(s) 237, 266, 354, 383
AlwNI CAGNNNCTG 1 cut(s) 1357
AoxI GGCC 3 cut(s) 117, 1296, 1625
ApaLI GTGCAC 1 cut(s) 1347
ApeKI GCWGC 3 cut(s) 1015, 1569, 1616
ApoI RAATTY 2 cut(s) 410, 613
ArsI GACNNNNNNTTYG 2 cut(s) 1643, 1675
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 6 cut(s) 118, 226, 343, 460, 1296, 1625
AsuHPI GGTGA 2 cut(s) 609, 1172
AvaII GGWCC 3 cut(s) 226, 343, 460
BaeGI GKGCMC 1 cut(s) 1351
BanII GRGCYC 2 cut(s) 14, 1422
Bbv12I GWGCWC 3 cut(s) 14, 1351, 1422
BbvI GCAGC 3 cut(s) 1002, 1556, 1603
BccI CCATC 4 cut(s) 281, 664, 1160, 1316
BciT130I CCWGG 2 cut(s) 817, 1195
BclI TGATCA 1 cut(s) 1710
BcoDI GTCTC 7 cut(s) 33, 177, 564, 884, 955, 1550, 1590
BcuI ACTAGT 1 cut(s) 920
BfaI CTAG 7 cut(s) 15, 300, 417, 441, 509, 621, 921
BfmI CTRYAG 2 cut(s) 352, 469
BisI GCNGC 3 cut(s) 1016, 1570, 1617
BlsI GCNGC 3 cut(s) 1017, 1571, 1618
Bme1390I CCNGG 2 cut(s) 817, 1195
Bme18I GGWCC 3 cut(s) 226, 343, 460
BmgT120I GGNCC 6 cut(s) 118, 226, 343, 460, 1296, 1625
BmiI GGNNCC 2 cut(s) 828, 854
BmrFI CCNGG 2 cut(s) 817, 1195
BmsI GCATC 7 cut(s) 568, 700, 745, 787, 940, 1581, 1613
BoxI GACNNNNGTC 1 cut(s) 1050
BpmI CTGGAG 3 cut(s) 426, 813, 1177
BsaBI GATNNNNATC 2 cut(s) 678, 1170
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 5 cut(s) 255, 372, 489, 730, 1520
Bsc4I CCNNNNNNNGG 4 cut(s) 713, 836, 886, 1465
Bse1I ACTGG 3 cut(s) 166, 654, 1362
Bse3DI GCAATG 1 cut(s) 782
Bse8I GATNNNNATC 2 cut(s) 678, 1170
BseBI CCWGG 2 cut(s) 817, 1195
BseDI CCNNGG 5 cut(s) 255, 372, 489, 730, 1520
BseGI GGATG 6 cut(s) 316, 931, 1222, 1306, 1366, 1555
BseJI GATNNNNATC 2 cut(s) 678, 1170
BseLI CCNNNNNNNGG 4 cut(s) 713, 836, 886, 1465
BseMI GCAATG 1 cut(s) 782
BseMII CTCAG 6 cut(s) 813, 968, 1116, 1504, 1628, 1665
BseNI ACTGG 3 cut(s) 166, 654, 1362
BseSI GKGCMC 1 cut(s) 1351
BseXI GCAGC 3 cut(s) 1002, 1556, 1603
BseYI CCCAGC 1 cut(s) 1441
Bsh1236I CGCG 1 cut(s) 793
BshFI GGCC 3 cut(s) 119, 1298, 1627
BsiHKAI GWGCWC 3 cut(s) 14, 1351, 1422
BslFI GGGAC 2 cut(s) 689, 853
BslI CCNNNNNNNGG 4 cut(s) 713, 836, 886, 1465
BsmAI GTCTC 7 cut(s) 33, 177, 564, 884, 955, 1550, 1590
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 689, 853
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 3 cut(s) 119, 1298, 1627
Bso31I GGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 3 cut(s) 14, 1351, 1422
Bsp143I GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
Bsp19I CCATGG 1 cut(s) 730
BspACI CCGC 1 cut(s) 1623
BspANI GGCC 3 cut(s) 119, 1298, 1627
BspCNI CTCAG 6 cut(s) 814, 967, 1117, 1503, 1629, 1666
BspFNI CGCG 1 cut(s) 793
BspLI GGNNCC 2 cut(s) 828, 854
BspPI GGATC 4 cut(s) 237, 266, 354, 383
BspQI GCTCTTC 1 cut(s) 2
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 1 cut(s) 782
BsrI ACTGG 3 cut(s) 166, 654, 1362
BssECI CCNNGG 5 cut(s) 255, 372, 489, 730, 1520
BssMI GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
BssT1I CCWWGG 5 cut(s) 255, 372, 489, 730, 1520
Bst2UI CCWGG 2 cut(s) 817, 1195
Bst4CI ACNGT 3 cut(s) 50, 1110, 1561
Bst6I CTCTTC 3 cut(s) 2, 954, 1281
BstAPI GCANNNNNTGC 1 cut(s) 977
BstC8I GCNNGC 6 cut(s) 44, 595, 715, 789, 1621, 1625
BstDEI CTNAG 8 cut(s) 822, 954, 1125, 1146, 1253, 1490, 1637, 1674
BstDSI CCRYGG 1 cut(s) 730
BstF5I GGATG 6 cut(s) 316, 931, 1222, 1306, 1366, 1555
BstFNI CGCG 1 cut(s) 793
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 7 cut(s) 245, 274, 362, 391, 508, 649, 1713
BstMAI GTCTC 7 cut(s) 33, 177, 564, 884, 955, 1550, 1590
BstMBI GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
BstMWI GCNNNNNNNGC 5 cut(s) 43, 797, 937, 977, 1451
BstNI CCWGG 2 cut(s) 817, 1195
BstNSI RCATGY 2 cut(s) 269, 386
BstPAI GACNNNNGTC 1 cut(s) 1050
BstSCI CCNGG 2 cut(s) 815, 1193
BstSFI CTRYAG 2 cut(s) 352, 469
BstSLI GKGCMC 1 cut(s) 1351
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 3 cut(s) 1002, 1556, 1603
BstX2I RGATCY 2 cut(s) 242, 359
BstXI CCANNNNNNTGG 1 cut(s) 1664
BstYI RGATCY 2 cut(s) 242, 359
BsuRI GGCC 3 cut(s) 119, 1298, 1627
BtgI CCRYGG 1 cut(s) 730
BtgZI GCGATG 2 cut(s) 1274, 1590
BtsCI GGATG 6 cut(s) 316, 931, 1222, 1306, 1366, 1555
BtsIMutI CAGTG 5 cut(s) 159, 529, 661, 1498, 1671
Cac8I GCNNGC 6 cut(s) 44, 595, 715, 789, 1621, 1625
CaiI CAGNNNCTG 1 cut(s) 1357
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 6 cut(s) 118, 226, 343, 460, 1296, 1625
CsiI ACCWGGT 1 cut(s) 815
Csp6I GTAC 4 cut(s) 252, 295, 369, 486
CspCI CAANNNNNGTGG 2 cut(s) 728, 763
CviAII CATG 7 cut(s) 266, 383, 529, 572, 701, 731, 1506
CviQI GTAC 4 cut(s) 252, 295, 369, 486
DdeI CTNAG 8 cut(s) 822, 954, 1125, 1146, 1253, 1490, 1637, 1674
DpnI GATC 7 cut(s) 244, 273, 361, 390, 507, 648, 1712
DpnII GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eam1104I CTCTTC 3 cut(s) 2, 954, 1281
EarI CTCTTC 3 cut(s) 2, 954, 1281
Ecl136II GAGCTC 2 cut(s) 12, 1420
Eco130I CCWWGG 5 cut(s) 255, 372, 489, 730, 1520
Eco24I GRGCYC 2 cut(s) 14, 1422
Eco31I GGTCTC 1 cut(s) 564
Eco47I GGWCC 3 cut(s) 226, 343, 460
Eco53kI GAGCTC 2 cut(s) 12, 1420
Eco57I CTGAAG 1 cut(s) 709
EcoICRI GAGCTC 2 cut(s) 12, 1420
EcoRI GAATTC 2 cut(s) 410, 613
EcoRII CCWGG 2 cut(s) 815, 1193
EcoT14I CCWWGG 5 cut(s) 255, 372, 489, 730, 1520
EcoT22I ATGCAT 1 cut(s) 1606
EcoT38I GRGCYC 2 cut(s) 14, 1422
ErhI CCWWGG 5 cut(s) 255, 372, 489, 730, 1520
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 7 cut(s) 269, 386, 532, 575, 704, 734, 1509
FaqI GGGAC 2 cut(s) 689, 853
FatI CATG 7 cut(s) 265, 382, 528, 571, 700, 730, 1505
FauI CCCGC 1 cut(s) 1616
FauNDI CATATG 1 cut(s) 835
FbaI TGATCA 1 cut(s) 1710
Fnu4HI GCNGC 3 cut(s) 1016, 1570, 1617
FokI GGATG 6 cut(s) 323, 918, 1209, 1293, 1373, 1562
FriOI GRGCYC 2 cut(s) 14, 1422
Fsp4HI GCNGC 3 cut(s) 1016, 1570, 1617
FspBI CTAG 7 cut(s) 15, 300, 417, 441, 509, 621, 921
GlaI GCGC 1 cut(s) 792
GluI GCNGC 3 cut(s) 1016, 1570, 1617
GsaI CCCAGC 1 cut(s) 1445
GsuI CTGGAG 3 cut(s) 426, 813, 1177
HaeIII GGCC 3 cut(s) 119, 1298, 1627
HhaI GCGC 1 cut(s) 793
Hin1II CATG 7 cut(s) 269, 386, 532, 575, 704, 734, 1509
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HinfI GANTC 6 cut(s) 65, 477, 584, 866, 964, 1249
HphI GGTGA 2 cut(s) 609, 1172
Hpy166II GTNNAC 5 cut(s) 297, 566, 1106, 1349, 1511
Hpy188III TCNNGA 7 cut(s) 246, 275, 363, 392, 509, 935, 956
Hpy8I GTNNAC 5 cut(s) 297, 566, 1106, 1349, 1511
HpyAV CCTTC 4 cut(s) 95, 109, 548, 904
HpyCH4III ACNGT 3 cut(s) 50, 1110, 1561
HpyCH4IV ACGT 2 cut(s) 842, 1392
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 797, 937, 977, 1451
HpyF3I CTNAG 8 cut(s) 822, 954, 1125, 1146, 1253, 1490, 1637, 1674
HpySE526I ACGT 2 cut(s) 842, 1392
Hsp92II CATG 7 cut(s) 269, 386, 532, 575, 704, 734, 1509
HspAI GCGC 1 cut(s) 791
Ksp22I TGATCA 1 cut(s) 1710
Kzo9I GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
LguI GCTCTTC 1 cut(s) 2
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 3 cut(s) 1002, 1556, 1603
LweI GCATC 7 cut(s) 568, 700, 745, 787, 940, 1581, 1613
MabI ACCWGGT 1 cut(s) 815
MaeI CTAG 7 cut(s) 15, 300, 417, 441, 509, 621, 921
MaeII ACGT 2 cut(s) 842, 1392
MaeIII GTNAC 3 cut(s) 599, 1376, 1691
MalI GATC 7 cut(s) 244, 273, 361, 390, 507, 648, 1712
MboI GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
MboII GAAGA 6 cut(s) 19, 186, 971, 998, 1069, 1298
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 2 cut(s) 242, 359
MhlI GDGCHC 3 cut(s) 14, 1351, 1422
MluCI AATT 6 cut(s) 410, 613, 870, 1039, 1065, 1130
MlyI GAGTC 3 cut(s) 59, 578, 1243
MmeI TCCRAC 1 cut(s) 867
Mph1103I ATGCAT 1 cut(s) 1606
MseI TTAA 3 cut(s) 762, 917, 1413
MslI CAYNNNNRTG 2 cut(s) 576, 741
MspA1I CMGCKG 1 cut(s) 1623
MspR9I CCNGG 2 cut(s) 817, 1195
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 2 cut(s) 817, 1195
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 5 cut(s) 43, 797, 937, 977, 1451
NcoI CCATGG 1 cut(s) 730
NdeI CATATG 1 cut(s) 835
NdeII GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
NlaIII CATG 7 cut(s) 269, 386, 532, 575, 704, 734, 1509
NlaIV GGNNCC 2 cut(s) 828, 854
NmuCI GTSAC 2 cut(s) 1376, 1691
NsiI ATGCAT 1 cut(s) 1606
NspI RCATGY 2 cut(s) 269, 386
PciI ACATGT 2 cut(s) 265, 382
PciSI GCTCTTC 1 cut(s) 2
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 3 cut(s) 477, 866, 964
PflFI GACNNNGTC 1 cut(s) 22
PflMI CCANNNNNTGG 2 cut(s) 836, 886
PkrI GCNGC 3 cut(s) 1017, 1571, 1618
PleI GAGTC 3 cut(s) 59, 578, 1243
PpsI GAGTC 3 cut(s) 59, 578, 1243
PscI ACATGT 2 cut(s) 265, 382
PshAI GACNNNNGTC 1 cut(s) 1050
Psp124BI GAGCTC 2 cut(s) 14, 1422
Psp6I CCWGG 2 cut(s) 815, 1193
PspFI CCCAGC 1 cut(s) 1441
PspGI CCWGG 2 cut(s) 815, 1193
PspN4I GGNNCC 2 cut(s) 828, 854
PspPI GGNCC 6 cut(s) 118, 226, 343, 460, 1296, 1625
PstNI CAGNNNCTG 1 cut(s) 1357
PsuI RGATCY 2 cut(s) 242, 359
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 4 cut(s) 253, 296, 370, 487
RsaNI GTAC 4 cut(s) 252, 295, 369, 486
RseI CAYNNNNRTG 2 cut(s) 576, 741
SacI GAGCTC 2 cut(s) 14, 1422
SapI GCTCTTC 1 cut(s) 2
SaqAI TTAA 3 cut(s) 762, 917, 1413
SatI GCNGC 3 cut(s) 1016, 1570, 1617
Sau3AI GATC 7 cut(s) 242, 271, 359, 388, 505, 646, 1710
Sau96I GGNCC 6 cut(s) 118, 226, 343, 460, 1296, 1625
SchI GAGTC 3 cut(s) 59, 578, 1243
ScrFI CCNGG 2 cut(s) 817, 1195
SduI GDGCHC 3 cut(s) 14, 1351, 1422
SexAI ACCWGGT 1 cut(s) 815
SfaNI GCATC 7 cut(s) 568, 700, 745, 787, 940, 1581, 1613
SfcI CTRYAG 2 cut(s) 352, 469
SinI GGWCC 3 cut(s) 226, 343, 460
SmiMI CAYNNNNRTG 2 cut(s) 576, 741
SpeI ACTAGT 1 cut(s) 920
Sse9I AATT 6 cut(s) 410, 613, 870, 1039, 1065, 1130
SsiI CCGC 1 cut(s) 1623
SspMI CTAG 7 cut(s) 15, 300, 417, 441, 509, 621, 921
SstI GAGCTC 2 cut(s) 14, 1422
StyD4I CCNGG 2 cut(s) 815, 1193
StyI CCWWGG 5 cut(s) 255, 372, 489, 730, 1520
TaaI ACNGT 3 cut(s) 50, 1110, 1561
TaiI ACGT 2 cut(s) 845, 1395
TaqI TCGA 1 cut(s) 1530
TaqII GACCGA 2 cut(s) 477, 1395
TasI AATT 6 cut(s) 410, 613, 870, 1039, 1065, 1130
TfiI GAWTC 3 cut(s) 477, 866, 964
Tru1I TTAA 3 cut(s) 762, 917, 1413
Tru9I TTAA 3 cut(s) 762, 917, 1413
TscAI CASTG 5 cut(s) 166, 529, 661, 1498, 1678
TseFI GTSAC 2 cut(s) 1376, 1691
TseI GCWGC 3 cut(s) 1015, 1569, 1616
Tsp45I GTSAC 2 cut(s) 1376, 1691
TspDTI ATGAA 7 cut(s) 20, 108, 762, 932, 1005, 1395, 1488
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 5 cut(s) 166, 529, 661, 1498, 1678
Tth111I GACNNNGTC 1 cut(s) 22
Van91I CCANNNNNTGG 2 cut(s) 836, 886
VneI GTGCAC 1 cut(s) 1347
VpaK11BI GGWCC 3 cut(s) 226, 343, 460
XapI RAATTY 2 cut(s) 410, 613
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 2 cut(s) 269, 386
XspI CTAG 7 cut(s) 15, 300, 417, 441, 509, 621, 921
Zsp2I ATGCAT 1 cut(s) 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.