pycom06g10150

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001166
Physical Location & Seq
Forward (+)
15530668 .. 15532180
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10150.4

Sequence Viewer

Length: 750 bp
ATGATCGATCTTGGAAATATAAAGATCATAGGAGATCATTTTAAAGCATCCGAACCCAATATCGACTCTTCTCAGGACATAGCTTCATCTATCTCGCGACCAAGAACTGATAATTATCCAAGGTCTCGCTTGTGGAGTAGGAGAAAATTAAGAAGTGCAGCATACATGTTGAAATTGAACTTCTTTAGTCTCCGCGGACTGCCATGGGGGTCTAGCACAGATGGCGAAGAAAAGGTTGAGCTAACTGCTGTAGAACTAGAATCACTTCGATCGGAGCTTGCTGATTTAGAACAGAAGGAAGCGCACTTGAAAGCTCAATTAGAACACGTTGATGAAGTTTTGCGATCTGCTCGTCTCGCTGGCTATTTGTACATCCGAACTAGGTGGAAACCACTACCAGGAGAACCTCCCCCTCTTGATGATACTGACGTTGATGACTGGCTTCCTCGCTTTGTTGTCTTGCAAGGGCCGTGCATTTTCTTCTTCTTGTTTTCCACAGATCTGAGTCCTCAAGACTCCACCCTCTTAGCTGATATTGTTGAAATAGGCCCCTTGCCAAGCATTACACGAGACGATGAAGGAACTCGACATGCATTTTGTATATTAACTCGTCAAGGACTGCGATTCGAGTGCTCAAGCGCTTCTAAGGAACAGGTGGACTCGTGGTTGTCAGCATTACGAACAGACTGCAAATTCGAGGGTTCAGATGCCGCCGCAGATCCAAACAACTCATGTGAGAAACAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00983 ko01100 ko01110 ko01130 ko04016 map00230 map00240 map00983 map01100 map01110 map01130 map04016
Pfam Domains
Protein Families
PH

Protein Analysis

250

Amino Acids

28.06

Weight (kDa)

4.99

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 97, 195
AciI CCGC 4 cut(s) 193, 195, 711, 714
AclWI GGATC 1 cut(s) 713
AcsI RAATTY 1 cut(s) 692
AfaI GTAC 1 cut(s) 371
AfeI AGCGCT 1 cut(s) 640
AfiI CCNNNNNNNGG 1 cut(s) 398
AflIII ACRYGT 2 cut(s) 165, 325
AgsI TTSAA 4 cut(s) 172, 178, 310, 542
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 5 cut(s) 83, 241, 277, 314, 530
AluI AGCT 5 cut(s) 83, 241, 277, 314, 530
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 4 cut(s) 129, 194, 359, 564
AlwI GGATC 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 640
AoxI GGCC 2 cut(s) 467, 547
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 692
ArsI GACNNNNNNTTYG 4 cut(s) 579, 611, 677, 709
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 304, 641
AspS9I GGNCC 2 cut(s) 467, 548
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BauI CACGAG 2 cut(s) 567, 661
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 1 cut(s) 170
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 454
BcgI CGANNNNNNTGC 4 cut(s) 612, 646, 669, 703
BciT130I CCWGG 1 cut(s) 399
BcoDI GTCTC 4 cut(s) 129, 194, 359, 564
BfaI CTAG 3 cut(s) 213, 257, 381
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 642
BglII AGATCT 1 cut(s) 499
BisI GCNGC 3 cut(s) 159, 711, 714
BlsI GCNGC 3 cut(s) 160, 712, 715
Bme1390I CCNGG 1 cut(s) 399
BmgT120I GGNCC 2 cut(s) 467, 548
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 56, 697
BpuEI CTTGAG 2 cut(s) 495, 619
Bsa29I ATCGAT 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 3 cut(s) 119, 193, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 443
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 119, 193, 203
BseGI GGATG 2 cut(s) 47, 372
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 86, 494
BseNI ACTGG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 2 cut(s) 97, 195
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 2 cut(s) 469, 549
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 635
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 4 cut(s) 129, 194, 359, 564
BsmBI CGTCTC 2 cut(s) 359, 564
BsnI GGCC 2 cut(s) 469, 549
Bso31I GGTCTC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 635
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
Bsp19I CCATGG 1 cut(s) 203
Bsp68I TCGCGA 1 cut(s) 97
BspACI CCGC 4 cut(s) 193, 195, 711, 714
BspANI GGCC 2 cut(s) 469, 549
BspCNI CTCAG 2 cut(s) 85, 495
BspDI ATCGAT 1 cut(s) 6
BspFNI CGCG 2 cut(s) 97, 195
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 1 cut(s) 713
BspTNI GGTCTC 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 119, 193, 203
BssMI GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
BssSI CACGAG 2 cut(s) 567, 661
BssT1I CCWWGG 2 cut(s) 119, 203
Bst2BI CACGAG 2 cut(s) 567, 661
Bst2UI CCWGG 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 73
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 279, 361
BstDEI CTNAG 4 cut(s) 72, 503, 526, 645
BstDSI CCRYGG 2 cut(s) 193, 203
BstF5I GGATG 2 cut(s) 47, 372
BstFNI CGCG 2 cut(s) 97, 195
BstH2I RGCGCY 1 cut(s) 642
BstHHI GCGC 2 cut(s) 304, 641
BstKTI GATC 8 cut(s) 6, 10, 27, 37, 272, 347, 502, 721
BstMAI GTCTC 4 cut(s) 129, 194, 359, 564
BstMBI GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 2 cut(s) 222, 356
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 2 cut(s) 169, 593
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 249
BstUI CGCG 2 cut(s) 97, 195
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 2 cut(s) 499, 718
BstYI RGATCY 2 cut(s) 499, 718
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 2 cut(s) 469, 549
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 2 cut(s) 193, 203
BtsCI GGATG 2 cut(s) 47, 372
BtuMI TCGCGA 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 279, 361
CfoI GCGC 2 cut(s) 304, 641
Cfr13I GGNCC 2 cut(s) 467, 548
Cfr42I CCGCGG 1 cut(s) 196
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 1 cut(s) 370
CviAII CATG 4 cut(s) 166, 204, 590, 732
CviJI RGCY 9 cut(s) 83, 241, 277, 314, 363, 442, 469, 530, 549
CviKI_1 RGCY 9 cut(s) 83, 241, 277, 314, 363, 442, 469, 530, 549
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 4 cut(s) 72, 503, 526, 645
DpnI GATC 8 cut(s) 5, 9, 26, 36, 271, 346, 501, 720
DpnII GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
DraI TTTAAA 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 2 cut(s) 119, 203
Eco31I GGTCTC 1 cut(s) 129
Eco47III AGCGCT 1 cut(s) 640
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 119, 203
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 2 cut(s) 119, 203
Esp3I CGTCTC 2 cut(s) 359, 564
FaeI CATG 4 cut(s) 169, 207, 593, 735
FaiI YATR 9 cut(s) 20, 29, 80, 163, 167, 205, 591, 602, 733
FatI CATG 4 cut(s) 165, 203, 589, 731
Fnu4HI GCNGC 3 cut(s) 159, 711, 714
FokI GGATG 2 cut(s) 34, 359
Fsp4HI GCNGC 3 cut(s) 159, 711, 714
FspBI CTAG 3 cut(s) 213, 257, 381
GlaI GCGC 2 cut(s) 303, 640
GluI GCNGC 3 cut(s) 159, 711, 714
HaeII RGCGCY 1 cut(s) 642
HaeIII GGCC 2 cut(s) 469, 549
HhaI GCGC 2 cut(s) 304, 641
Hin1II CATG 4 cut(s) 169, 207, 593, 735
Hin6I GCGC 2 cut(s) 302, 639
HinP1I GCGC 2 cut(s) 302, 639
HinfI GANTC 6 cut(s) 65, 260, 505, 515, 624, 659
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 5 cut(s) 52, 274, 377, 504, 706
Hpy188III TCNNGA 4 cut(s) 74, 96, 416, 512
Hpy8I GTNNAC 1 cut(s) 658
HpyAV CCTTC 2 cut(s) 289, 572
HpyCH4IV ACGT 2 cut(s) 327, 429
HpyCH4V TGCA 5 cut(s) 158, 463, 474, 593, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 356
HpyF3I CTNAG 4 cut(s) 72, 503, 526, 645
HpySE526I ACGT 2 cut(s) 327, 429
Hsp92II CATG 4 cut(s) 169, 207, 593, 735
HspAI GCGC 2 cut(s) 302, 639
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 6 cut(s) 59, 345, 384, 411, 424, 638
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 2 cut(s) 56, 697
MaeI CTAG 3 cut(s) 213, 257, 381
MaeII ACGT 2 cut(s) 327, 429
MalI GATC 8 cut(s) 5, 9, 26, 36, 271, 346, 501, 720
MboI GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
MboII GAAGA 4 cut(s) 60, 239, 472, 475
MflI RGATCY 2 cut(s) 499, 718
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 5 cut(s) 112, 146, 173, 317, 692
MlyI GAGTC 4 cut(s) 59, 509, 514, 653
MnlI CCTC 6 cut(s) 417, 423, 456, 519, 533, 691
Mph1103I ATGCAT 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 4 cut(s) 42, 149, 605, 748
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 2 cut(s) 97, 195
MwoI GCNNNNNNNGC 2 cut(s) 222, 356
NcoI CCATGG 1 cut(s) 203
NdeII GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
NlaIII CATG 4 cut(s) 169, 207, 593, 735
NlaIV GGNNCC 1 cut(s) 550
NruI TCGCGA 1 cut(s) 97
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 2 cut(s) 169, 593
PciI ACATGT 1 cut(s) 165
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 260, 624
PkrI GCNGC 3 cut(s) 160, 712, 715
Ple19I CGATCG 1 cut(s) 272
PleI GAGTC 4 cut(s) 59, 509, 513, 653
PpsI GAGTC 4 cut(s) 59, 509, 513, 653
PscI ACATGT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 2 cut(s) 467, 548
PsuI RGATCY 2 cut(s) 499, 718
PvuI CGATCG 1 cut(s) 272
RruI TCGCGA 1 cut(s) 97
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 330
SacII CCGCGG 1 cut(s) 196
SaqAI TTAA 4 cut(s) 42, 149, 605, 748
SatI GCNGC 3 cut(s) 159, 711, 714
Sau3AI GATC 8 cut(s) 3, 7, 24, 34, 269, 344, 499, 718
Sau96I GGNCC 2 cut(s) 467, 548
SchI GAGTC 4 cut(s) 59, 509, 514, 653
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 1 cut(s) 635
SfaNI GCATC 2 cut(s) 56, 697
SfcI CTRYAG 1 cut(s) 249
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 510, 634
SmoI CTYRAG 2 cut(s) 510, 634
Sse9I AATT 5 cut(s) 112, 146, 173, 317, 692
SsiI CCGC 4 cut(s) 193, 195, 711, 714
SspMI CTAG 3 cut(s) 213, 257, 381
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 2 cut(s) 119, 203
TaiI ACGT 2 cut(s) 330, 432
TaqI TCGA 6 cut(s) 6, 63, 268, 586, 627, 696
TasI AATT 5 cut(s) 112, 146, 173, 317, 692
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 713, 716
TfiI GAWTC 2 cut(s) 260, 624
Tru1I TTAA 4 cut(s) 42, 149, 605, 748
Tru9I TTAA 4 cut(s) 42, 149, 605, 748
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 3 cut(s) 75, 348, 591
XapI RAATTY 1 cut(s) 692
XceI RCATGY 2 cut(s) 169, 593
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 3 cut(s) 213, 257, 381
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.