Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001683

Physical Location & Seq

Genomic Coordinates Reverse (-)

33587486 .. 33592104

Gene Structure 4619 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1202700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2115 bp

ATGCGACAACGACTCGATCTTATGCATGATACTGAGGTTGTCTGGGATCCATATCATGGTCAGAGAGGACATCATTCTTTACCAAAGATGGCATTCTATACTGGTCTCCTCAAGTGTGTGGATATTGTGGAGCCTTATTATGTTGATAGGGTTCTACGACAGTTTAGCCTTGTTCAGACAATTCCCCGAATGACATATACCCCTTCTCGAGTTCAACGGAGCACTGATACAAAAATGTACAAGGTTGTGCATACGTACTTTGATGGGATTTGGCAAAGGCGTGAATATCACCTATTGGGACCAGAAAACCTTAGCAGGGAGGTTATAGCCCCATCAGATTGTGATCCTGGGTACCACTCATGGTATCGTAAAATCACCCATCCATACGTGCATCACCCTGATCATAGGTCTGCTGCCATACGGCCACATGACATAAATGATCCACATCAGCGCATAGCACGTCTTCTAGCCATCATGGAGCCTATTTCACAGTCTAATCTTCGTTGTGCTTGTAAATTTTACGAGGCATTTGAGCAAGTCCGGTCAATTTTGCAAGGGTCTGAGGCAGAAATTGCAGCAACAGCAGCAGCAGCTCAAGCAGAACCAGCAACACCTGTTCCATCTGCATCTACAACCCAAAACATTGCAGACCCAGCAGCACCTGTTTCATCCGCATCTGCCACTCAAGACGTTGCAGAACCAGCAGCAGCTCAAGATGTTGCAGAACCTGCAACACCTGTTCCATCTACATCTGCAACTCAAGACGTTGCAGAACCAGCAGCTGCTCAAGACGTTGCAGGACGAGTAGCACTGCCGCTGGTCTTCGGTTCATCGTCTTCCTTTGTCAAAAACTCTGAATCCAAAGACAACCCATTGTCCTTGCCGCCGCCACCTGAAACCCCAATTTTCTGGCCGCCTGACGGAACGCTCAGCCTTGAATGGGTTCGGAACCTAATGTCCACTTTCGATTGGGCTTCTAGAAATCTTGAACCAAACCAATTTCCGGATGTATTTCCTATGGAGGTTTTTGATAGCTTGGTCCTCTGCGCCTTCAATATCCTCCACAAAGAGGCAAATTGCATTGCCGTCGACCAGTTAGGTATGGAATCCACTGTTGTCGTTGTTGGAGACATTCACGGTCAGCTGCACGATCTCCTTTTCCTTCTAAATGATGCTGGATTTCCTTCAGAAAATCGATTATACGTCTTCAATGGCGATTATGTTGATAGAGGGGCTTGGGGGATCGAAACCTTCTTGATTTTATTGGCTTGGAAGGTGTTTTTGCCCAAGAGCGTTTATCTTCTTAGAGGAAATCACGAATCGAAATACTGCACTTCCGTTTACGGATTTGAAAAGGAAGTGTTGACAAAGTATGGAGGAGACAAAGGGAAGGATGTGTACCAAAAATGTCTCGAGTGCTTTAAACGTCTCCCTTTGACATCCATTATCGGGAAATACGTATACACTGCTCATGGTGGGCTGTTTCGCAGCATGGTTGCATACCCCAAGAGATCAAAGGGAAAGAAGAGGAGGAAAATATCTTTCGTTCCTGAACTGAGCAGTAAATTGTCTCTTGGCACGTTTGAAGAATTGAAGAAGGCTCAAAGATCGGTTCTTAATCCTCCGTGGATGGGTTCAAACTTGATTCCTGGTGACGTGCTATGGTCAGATCCCTCCATGGATCCCGGCCTTTCTTTGAATGAAGAGAGAGGCATTGGTTTGCTTTGGGGCCCTGACTGCACTGAGGAGTTTCTCAAGAAGTTTCAACTCAAGTTGATTATTAGATCGCATGAAGGCCCTGATGCACGGGACAAGAGGCCAGGTCTTGGTGGAATGGATAATGGGTTCACCATAGATCACACTGTGGAGTCAGGGAAGCTCATTACTTTGTTTAGTGCTCCAGACTACCCACAATTTCAGGCAACAGAAGATAGGTACAGAAATAAAGGAGCTTACATCGTTCTGGAACCCCCTAATTTTGACGATCCTGTTATCCACAGCTTTGAAGCAATCACTCCAAGACCTCAGGCAAATCCCTTTTATGACTTTGAAGAAGTGGCTGATTCTGATCAAGAGTTGGACTTTGCATCGCCATCGATGGAGACTAGCCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009637 GO:0009785 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023052 GO:0030522 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0104004 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04922 map04010 map04922

Pfam Domains

Protein Families

Metallophos PMD

Protein Analysis

705

Amino Acids

78.87

Weight (kDa)

5.59

Isoelectric Point (pI)

47.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMD	PF10536	1 - 122	4.6e-10	Plant mobile domain
Metallophos	PF00149	371 - 598	1.1e-28	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001683)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g02784 FvH4_5g13891 FvH4_6g36961
malus_domestica	MD06G1202700.v1.1 MD17G1027800.v1.1 MD17G1028300.v1.1
pyrus_communis	pycom06g17890 pycom06g17910 pycom06g17920 pycom17g02270 pycom17g02380 pycom17g02450
rosa_chinensis	RchiOBHm_Chr1g0332031 RchiOBHm_Chr5g0043391 RchiOBHm_Chr6g0252591
rosa_laevigata	RLG00000029816 RLG00000030362
rosa_multiflora	Rmu_sc0000431.1_g000002 Rmu_sc0001114.1_g000010 Rmu_sc0003724.1_g000008 Rmu_sc0003994.1_g000024 Rmu_sc0004755.1_g000019 Rmu_sc0005212.1_g000002 Rmu_sc0007943.1_g000002
rosa_rugosa	Rorug02G0188700 Rorug02G0515000 Rorug04G0360500 Rorug04G0360500 Rorug04G0360600 Rorug05G0596000 Rorug06G0366600 Rorug06G0366600 Rorug07G0140000
rosa_samantha	Rh1CG084500 Rh2AG079400 Rh2AG243900 Rh2AG367300 Rh2DG078500 Rh3BG356900 Rh3CG353700 Rh3DG356300 Rh3DG356400 Rh4CG179300 Rh4DG064600 Rh5BG277000 Rh5BG297900 Rh5DG307300
rosa_wichuraiana	Rw3G028120 Rw4G015280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	736
Acc65I	GGTACC	1 cut(s)	351
AccB1I	GGYRCC	1 cut(s)	351
AccB7I	CCANNNNNTGG	2 cut(s)	56, 1826
AccI	GTMKAC	2 cut(s)	1089, 1461
AccIII	TCCGGA	1 cut(s)	1003
AciI	CCGC	5 cut(s)	672, 815, 884, 887, 914
AclWI	GGATC	9 cut(s)	41, 54, 338, 434, 1250, 1664, 1676, 1689, 1979
AcoI	YGGCCR	2 cut(s)	422, 911
AcsI	RAATTY	1 cut(s)	515
AcuI	CTGAAG	1 cut(s)	1170
AdeI	CACNNNGTG	1 cut(s)	1864
AfaI	GTAC	5 cut(s)	239, 257, 353, 1400, 1937
AfiI	CCNNNNNNNGG	9 cut(s)	56, 316, 920, 940, 1003, 1069, 1449, 1631, 1826
AjiI	CACGTC	2 cut(s)	461, 1657
AjnI	CCWGG	3 cut(s)	346, 1648, 1819
AloI	GAACNNNNNNTCC	4 cut(s)	941, 973, 1829, 1861
AluBI	AGCT	8 cut(s)	593, 710, 782, 1035, 1144, 1879, 1952, 2001
AluI	AGCT	8 cut(s)	593, 710, 782, 1035, 1144, 1879, 1952, 2001
Alw21I	GWGCWC	2 cut(s)	224, 1900
Alw26I	GTCTC	7 cut(s)	110, 1122, 1374, 1415, 1433, 1575, 2096
AlwI	GGATC	9 cut(s)	41, 54, 338, 434, 1250, 1664, 1676, 1689, 1979
AlwNI	CAGNNNCTG	3 cut(s)	662, 728, 782
Ama87I	CYCGRG	2 cut(s)	207, 1412
Aor13HI	TCCGGA	1 cut(s)	1003
AoxI	GGCC	6 cut(s)	422, 911, 1687, 1729, 1795, 1817
ApaI	GGGCCC	1 cut(s)	1733
ApoI	RAATTY	1 cut(s)	515
Asp700I	GAANNNNTTC	1 cut(s)	942
Asp718I	GGTACC	1 cut(s)	351
AspLEI	GCGC	2 cut(s)	453, 1049
AspS9I	GGNCC	5 cut(s)	299, 1039, 1729, 1730, 1796
AsuC2I	CCSGG	1 cut(s)	1686
AsuHPI	GGTGA	5 cut(s)	281, 367, 386, 1664, 1840
AvaI	CYCGRG	2 cut(s)	207, 1412
AvaII	GGWCC	2 cut(s)	299, 1039
AxyI	CCTNAGG	1 cut(s)	2025
BaeGI	GKGCMC	1 cut(s)	1733
BaeI	ACNNNNGTAYC	2 cut(s)	21, 54
BamHI	GGATCC	2 cut(s)	46, 1681
BanI	GGYRCC	1 cut(s)	351
BanII	GRGCYC	1 cut(s)	1733
BarI	GAAGNNNNNNTAC	2 cut(s)	1382, 1414
BbsI	GAAGAC	4 cut(s)	455, 814, 828, 1198
Bbv12I	GWGCWC	2 cut(s)	224, 1900
BceAI	ACGGC	2 cut(s)	437, 1070
BcgI	CGANNNNNNTGC	4 cut(s)	1069, 1103, 2076, 2110
BciT130I	CCWGG	3 cut(s)	348, 1650, 1821
BclI	TGATCA	2 cut(s)	400, 2068
BcnI	CCSGG	1 cut(s)	1686
BcoDI	GTCTC	7 cut(s)	110, 1122, 1374, 1415, 1433, 1575, 2096
BfaI	CTAG	3 cut(s)	467, 978, 2106
BfuAI	ACCTGC	1 cut(s)	736
BlpI	GCTNAGC	1 cut(s)	929
Bme1390I	CCNGG	4 cut(s)	348, 1650, 1686, 1821
Bme18I	GGWCC	2 cut(s)	299, 1039
BmeT110I	CYCGRG	2 cut(s)	207, 1412
BmgBI	CACGTC	2 cut(s)	461, 1657
BmgT120I	GGNCC	5 cut(s)	299, 1039, 1729, 1730, 1796
BmrFI	CCNGG	4 cut(s)	348, 1650, 1686, 1821
BmsI	GCATC	6 cut(s)	400, 635, 683, 1162, 1792, 2096
BpiI	GAAGAC	4 cut(s)	455, 814, 828, 1198
BpmI	CTGGAG	1 cut(s)	1884
Bpu10I	CCTNAGC	1 cut(s)	311
Bpu1102I	GCTNAGC	1 cut(s)	929
BpuEI	CTTGAG	8 cut(s)	95, 579, 669, 696, 744, 771, 1739, 1754
BpuMI	CCSGG	1 cut(s)	1686
Bsa29I	ATCGAT	2 cut(s)	1195, 2096
BsaAI	YACGTR	3 cut(s)	255, 388, 1459
BsaBI	GATNNNNATC	4 cut(s)	51, 342, 444, 2067
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	3 cut(s)	347, 1625, 1677
BsaWI	WCCGGW	2 cut(s)	540, 1003
Bsc4I	CCNNNNNNNGG	9 cut(s)	56, 316, 920, 940, 1003, 1069, 1449, 1631, 1826
Bse1I	ACTGG	2 cut(s)	106, 1093
Bse21I	CCTNAGG	1 cut(s)	2025
Bse3DI	GCAATG	2 cut(s)	642, 1080
Bse8I	GATNNNNATC	4 cut(s)	51, 342, 444, 2067
BseAI	TCCGGA	1 cut(s)	1003
BseBI	CCWGG	3 cut(s)	348, 1650, 1821
BseCI	ATCGAT	2 cut(s)	1195, 2096
BseDI	CCNNGG	3 cut(s)	347, 1625, 1677
BseGI	GGATG	6 cut(s)	379, 668, 1012, 1399, 1439, 1635
BseJI	GATNNNNATC	4 cut(s)	51, 342, 444, 2067
BseLI	CCNNNNNNNGG	9 cut(s)	56, 316, 920, 940, 1003, 1069, 1449, 1631, 1826
BseMI	GCAATG	2 cut(s)	642, 1080
BseMII	CTCAG	6 cut(s)	24, 552, 943, 1547, 1734, 2039
BseNI	ACTGG	2 cut(s)	106, 1093
BseRI	GAGGAG	4 cut(s)	98, 1392, 1543, 1760
BseSI	GKGCMC	1 cut(s)	1733
BseYI	CCCAGC	1 cut(s)	652
BsgI	GTGCAG	3 cut(s)	1130, 1315, 1723
BshFI	GGCC	6 cut(s)	424, 913, 1689, 1731, 1797, 1819
BshNI	GGYRCC	1 cut(s)	351
BshVI	ATCGAT	2 cut(s)	1195, 2096
BsiHKAI	GWGCWC	2 cut(s)	224, 1900
BsiHKCI	CYCGRG	2 cut(s)	207, 1412
BsiSI	CCGG	3 cut(s)	541, 1004, 1686
BslFI	GGGAC	2 cut(s)	312, 1823
BslI	CCNNNNNNNGG	9 cut(s)	56, 316, 920, 940, 1003, 1069, 1449, 1631, 1826
BsmAI	GTCTC	7 cut(s)	110, 1122, 1374, 1415, 1433, 1575, 2096
BsmBI	CGTCTC	1 cut(s)	1433
BsmFI	GGGAC	2 cut(s)	312, 1823
BsmI	GAATGC	1 cut(s)	92
BsnI	GGCC	6 cut(s)	424, 913, 1689, 1731, 1797, 1819
Bso31I	GGTCTC	1 cut(s)	110
BsoBI	CYCGRG	2 cut(s)	207, 1412
Bsp120I	GGGCCC	1 cut(s)	1729
Bsp1286I	GDGCHC	3 cut(s)	224, 1733, 1900
Bsp13I	TCCGGA	1 cut(s)	1003
Bsp1407I	TGTACA	1 cut(s)	237
Bsp1720I	GCTNAGC	1 cut(s)	929
Bsp19I	CCATGG	1 cut(s)	1677
BspACI	CCGC	5 cut(s)	672, 815, 884, 887, 914
BspANI	GGCC	6 cut(s)	424, 913, 1689, 1731, 1797, 1819
BspCNI	CTCAG	6 cut(s)	25, 553, 942, 1548, 1735, 2038
BspDI	ATCGAT	2 cut(s)	1195, 2096
BspEI	TCCGGA	1 cut(s)	1003
BspMI	ACCTGC	1 cut(s)	736
BspPI	GGATC	9 cut(s)	41, 54, 338, 434, 1250, 1664, 1676, 1689, 1979
BspT107I	GGYRCC	1 cut(s)	351
BspTNI	GGTCTC	1 cut(s)	110
BsrDI	GCAATG	2 cut(s)	642, 1080
BsrGI	TGTACA	1 cut(s)	237
BsrI	ACTGG	2 cut(s)	106, 1093
BssECI	CCNNGG	3 cut(s)	347, 1625, 1677
BssNAI	GTATAC	1 cut(s)	1462
BssT1I	CCWWGG	1 cut(s)	1677
Bst1107I	GTATAC	1 cut(s)	1462
Bst2UI	CCWGG	3 cut(s)	348, 1650, 1821
Bst4CI	ACNGT	5 cut(s)	162, 492, 1114, 1139, 1864
Bst6I	CTCTTC	2 cut(s)	1520, 1698
BstAPI	GCANNNNNTGC	2 cut(s)	572, 728
BstAUI	TGTACA	1 cut(s)	237
BstBAI	YACGTR	3 cut(s)	255, 388, 1459
BstDEI	CTNAG	8 cut(s)	33, 311, 561, 929, 1304, 1556, 1743, 2025
BstDSI	CCRYGG	2 cut(s)	1625, 1677
BstF5I	GGATG	6 cut(s)	379, 668, 1012, 1399, 1439, 1635
BstHHI	GCGC	2 cut(s)	453, 1049
BstMAI	GTCTC	7 cut(s)	110, 1122, 1374, 1415, 1433, 1575, 2096
BstNI	CCWGG	3 cut(s)	348, 1650, 1821
BstSCI	CCNGG	4 cut(s)	346, 1648, 1684, 1819
BstSLI	GKGCMC	1 cut(s)	1733
BstSNI	TACGTA	2 cut(s)	255, 1459
BstV2I	GAAGAC	4 cut(s)	455, 814, 828, 1198
BstX2I	RGATCY	3 cut(s)	46, 1669, 1681
BstXI	CCANNNNNNTGG	1 cut(s)	909
BstYI	RGATCY	3 cut(s)	46, 1669, 1681
BstZ17I	GTATAC	1 cut(s)	1462
Bsu15I	ATCGAT	2 cut(s)	1195, 2096
Bsu36I	CCTNAGG	1 cut(s)	2025
BsuRI	GGCC	6 cut(s)	424, 913, 1689, 1731, 1797, 1819
BsuTUI	ATCGAT	2 cut(s)	1195, 2096
BtgI	CCRYGG	2 cut(s)	1625, 1677
BtgZI	GCGATG	1 cut(s)	2073
BtrI	CACGTC	2 cut(s)	461, 1657
BtsCI	GGATG	6 cut(s)	379, 668, 1012, 1399, 1439, 1635
BtsI	GCAGTG	2 cut(s)	809, 1464
BtsIMutI	CAGTG	6 cut(s)	222, 809, 1110, 1464, 1740, 1860
BveI	ACCTGC	1 cut(s)	736
CaiI	CAGNNNCTG	3 cut(s)	662, 728, 782
CfoI	GCGC	2 cut(s)	453, 1049
Cfr13I	GGNCC	5 cut(s)	299, 1039, 1729, 1730, 1796
ClaI	ATCGAT	2 cut(s)	1195, 2096
Csp6I	GTAC	5 cut(s)	238, 256, 352, 1399, 1936
CviAII	CATG	9 cut(s)	26, 56, 360, 428, 475, 1472, 1492, 1678, 1790
CviQI	GTAC	5 cut(s)	238, 256, 352, 1399, 1936
DdeI	CTNAG	8 cut(s)	33, 311, 561, 929, 1304, 1556, 1743, 2025
DraI	TTTAAA	1 cut(s)	1423
DraIII	CACNNNGTG	1 cut(s)	1864
EaeI	YGGCCR	2 cut(s)	422, 911
Eam1104I	CTCTTC	2 cut(s)	1520, 1698
EarI	CTCTTC	2 cut(s)	1520, 1698
Eco105I	TACGTA	2 cut(s)	255, 1459
Eco130I	CCWWGG	1 cut(s)	1677
Eco24I	GRGCYC	1 cut(s)	1733
Eco31I	GGTCTC	1 cut(s)	110
Eco47I	GGWCC	2 cut(s)	299, 1039
Eco57I	CTGAAG	1 cut(s)	1170
Eco81I	CCTNAGG	1 cut(s)	2025
Eco88I	CYCGRG	2 cut(s)	207, 1412
EcoO109I	RGGNCCY	3 cut(s)	1729, 1730, 1796
EcoRII	CCWGG	3 cut(s)	346, 1648, 1819
EcoT14I	CCWWGG	1 cut(s)	1677
EcoT22I	ATGCAT	1 cut(s)	27
EcoT38I	GRGCYC	1 cut(s)	1733
ErhI	CCWWGG	1 cut(s)	1677
Esp3I	CGTCTC	1 cut(s)	1433
FaeI	CATG	9 cut(s)	29, 59, 363, 431, 478, 1475, 1495, 1681, 1793
FaqI	GGGAC	2 cut(s)	312, 1823
FatI	CATG	9 cut(s)	25, 55, 359, 427, 474, 1471, 1491, 1677, 1789
FbaI	TGATCA	2 cut(s)	400, 2068
FblI	GTMKAC	2 cut(s)	1089, 1461
FokI	GGATG	6 cut(s)	366, 655, 1019, 1406, 1426, 1642
FriOI	GRGCYC	1 cut(s)	1733
FspBI	CTAG	3 cut(s)	467, 978, 2106
GlaI	GCGC	2 cut(s)	452, 1048
GsaI	CCCAGC	1 cut(s)	656
GsuI	CTGGAG	1 cut(s)	1884
HaeIII	GGCC	6 cut(s)	424, 913, 1689, 1731, 1797, 1819
HapII	CCGG	3 cut(s)	541, 1004, 1686
HhaI	GCGC	2 cut(s)	453, 1049
Hin1II	CATG	9 cut(s)	29, 59, 363, 431, 478, 1475, 1495, 1681, 1793
Hin6I	GCGC	2 cut(s)	451, 1047
HinP1I	GCGC	2 cut(s)	451, 1047
HincII	GTYRAC	2 cut(s)	1090, 1365
HindII	GTYRAC	2 cut(s)	1090, 1365
HinfI	GANTC	7 cut(s)	12, 857, 1106, 1319, 1645, 1868, 2063
HpaII	CCGG	3 cut(s)	541, 1004, 1686
HphI	GGTGA	5 cut(s)	281, 367, 386, 1664, 1840
Hpy166II	GTNNAC	7 cut(s)	960, 1090, 1342, 1365, 1399, 1462, 1848
Hpy188I	TCNGA	9 cut(s)	63, 177, 337, 562, 856, 948, 1189, 1669, 2068
Hpy8I	GTNNAC	7 cut(s)	960, 1090, 1342, 1365, 1399, 1462, 1848
Hpy99I	CGWCG	1 cut(s)	1091
HpyAV	CCTTC	9 cut(s)	213, 1060, 1172, 1194, 1261, 1267, 1384, 1591, 1787
HpyCH4III	ACNGT	5 cut(s)	162, 492, 1114, 1139, 1864
HpyF3I	CTNAG	8 cut(s)	33, 311, 561, 929, 1304, 1556, 1743, 2025
Hsp92II	CATG	9 cut(s)	29, 59, 363, 431, 478, 1475, 1495, 1681, 1793
HspAI	GCGC	2 cut(s)	451, 1047
Kpn2I	TCCGGA	1 cut(s)	1003
KpnI	GGTACC	1 cut(s)	355
Ksp22I	TGATCA	2 cut(s)	400, 2068
LmnI	GCTCC	5 cut(s)	130, 219, 478, 1903, 1949
LweI	GCATC	6 cut(s)	400, 635, 683, 1162, 1792, 2096
MaeI	CTAG	3 cut(s)	467, 978, 2106
MaeIII	GTNAC	1 cut(s)	1652
MflI	RGATCY	3 cut(s)	46, 1669, 1681
MhlI	GDGCHC	3 cut(s)	224, 1733, 1900
MlyI	GAGTC	2 cut(s)	6, 1877
MmeI	TCCRAC	2 cut(s)	1105, 2058
Mph1103I	ATGCAT	1 cut(s)	27
MroI	TCCGGA	1 cut(s)	1003
MroXI	GAANNNNTTC	1 cut(s)	942
MseI	TTAA	2 cut(s)	1422, 1617
MspA1I	CMGCKG	3 cut(s)	782, 817, 1144
MspI	CCGG	3 cut(s)	541, 1004, 1686
MspR9I	CCNGG	4 cut(s)	348, 1650, 1686, 1821
Mva1269I	GAATGC	1 cut(s)	92
MvaI	CCWGG	3 cut(s)	348, 1650, 1821
NciI	CCSGG	1 cut(s)	1686
NcoI	CCATGG	1 cut(s)	1677
NlaIII	CATG	9 cut(s)	29, 59, 363, 431, 478, 1475, 1495, 1681, 1793
NmuCI	GTSAC	1 cut(s)	1652
NsiI	ATGCAT	1 cut(s)	27
PaeR7I	CTCGAG	2 cut(s)	207, 1412
PcsI	WCGNNNNNNNCGW	1 cut(s)	1455
PctI	GAATGC	1 cut(s)	92
PdmI	GAANNNNTTC	1 cut(s)	942
PfeI	GAWTC	5 cut(s)	857, 1106, 1319, 1645, 2063
PflMI	CCANNNNNTGG	2 cut(s)	56, 1826
PleI	GAGTC	2 cut(s)	6, 1876
PpsI	GAGTC	2 cut(s)	6, 1876
Ppu21I	YACGTR	3 cut(s)	255, 388, 1459
Psp6I	CCWGG	3 cut(s)	346, 1648, 1819
PspFI	CCCAGC	1 cut(s)	652
PspGI	CCWGG	3 cut(s)	346, 1648, 1819
PspOMI	GGGCCC	1 cut(s)	1729
PspPI	GGNCC	5 cut(s)	299, 1039, 1729, 1730, 1796
PstNI	CAGNNNCTG	3 cut(s)	662, 728, 782
PsuI	RGATCY	3 cut(s)	46, 1669, 1681
PvuII	CAGCTG	2 cut(s)	782, 1144
RsaI	GTAC	5 cut(s)	239, 257, 353, 1400, 1937
RsaNI	GTAC	5 cut(s)	238, 256, 352, 1399, 1936
SalI	GTCGAC	1 cut(s)	1088
SaqAI	TTAA	2 cut(s)	1422, 1617
Sau96I	GGNCC	5 cut(s)	299, 1039, 1729, 1730, 1796
SchI	GAGTC	2 cut(s)	6, 1877
ScrFI	CCNGG	4 cut(s)	348, 1650, 1686, 1821
SduI	GDGCHC	3 cut(s)	224, 1733, 1900
SfaNI	GCATC	6 cut(s)	400, 635, 683, 1162, 1792, 2096
Sfr274I	CTCGAG	2 cut(s)	207, 1412
SinI	GGWCC	2 cut(s)	299, 1039
SlaI	CTCGAG	2 cut(s)	207, 1412
SnaBI	TACGTA	2 cut(s)	255, 1459
SsiI	CCGC	5 cut(s)	672, 815, 884, 887, 914
SspMI	CTAG	3 cut(s)	467, 978, 2106
StyD4I	CCNGG	4 cut(s)	346, 1648, 1684, 1819
StyI	CCWWGG	1 cut(s)	1677
TaaI	ACNGT	5 cut(s)	162, 492, 1114, 1139, 1864
TaqI	TCGA	9 cut(s)	15, 208, 966, 1089, 1195, 1245, 1322, 1413, 2096
TatI	WGTACW	1 cut(s)	237
TauI	GCSGC	4 cut(s)	817, 886, 889, 916
TfiI	GAWTC	5 cut(s)	857, 1106, 1319, 1645, 2063
Tru1I	TTAA	2 cut(s)	1422, 1617
Tru9I	TTAA	2 cut(s)	1422, 1617
TscAI	CASTG	6 cut(s)	229, 816, 1117, 1471, 1747, 1867
TseFI	GTSAC	1 cut(s)	1652
Tsp45I	GTSAC	1 cut(s)	1652
TspDTI	ATGAA	4 cut(s)	657, 819, 1716, 1806
TspGWI	ACGGA	5 cut(s)	232, 936, 1327, 1359, 1614
TspRI	CASTG	6 cut(s)	229, 816, 1117, 1471, 1747, 1867
Van91I	CCANNNNNTGG	2 cut(s)	56, 1826
VpaK11BI	GGWCC	2 cut(s)	299, 1039
XapI	RAATTY	1 cut(s)	515
XbaI	TCTAGA	1 cut(s)	977
XhoI	CTCGAG	2 cut(s)	207, 1412
XmiI	GTMKAC	2 cut(s)	1089, 1461
XmnI	GAANNNNTTC	1 cut(s)	942
XspI	CTAG	3 cut(s)	467, 978, 2106
Zsp2I	ATGCAT	1 cut(s)	27

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.