pycom06g17920

magnesium ion export from mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001683
Physical Location & Seq
Reverse (-)
21846791 .. 21848650
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17920.1

Sequence Viewer

Length: 1095 bp
ATGTCCTTTGCATTTGTACAAAATCTGTCTAAAAGTCTAGAACTTTCCCACTTCACATCTCTTTCTCGACTTCGAAGCGCACCACTTCACTTTTCCGTGCTTTCTCCGTCGCCAAGCCAGTGGCCACCACGCACCACCACCAGACGACTCCTGGCGGAGCCACCGCGAGGTCCACCACTACGTCTCGGTCTGGTATTTCCAACACGCCGTTTGTTCCTTTTTTCTTCGCACCTCAGCCACCACCACCTATCCCTTCCACCACCTCCACACTCCGTCTCCCCCGGCCACCACCTCCGCGGGTCCACGTCTTCTCTCTGGTCATCACTGACTGGATTGCTTCTGGGTTGTTGCATACGACGGAAGTGCGGGGATACTGTCCATATGGATGCTGAAGGTTCTAGTGGTAAGAGGCCCACTTCCTCGAAAAGGAAACGGAAAACCTCTACAGATGATGCACAGCTAGATTCTCAAACGACTCTATGGGGAGACAAGATTTTGAAGGATCCATTTCCAGGAGGTCCGGAGGATCCGAGGGTGCTTAGGAGTTTCCCAAGTCATGTAGCTGCTGCTATTTGGCATAATGTGGAACGTGAACCCCTTAGGGTGTTCAATCACACTTGTAAGCTTCAAGCATGGAAATTTGACCCATCCGTTTCTAACTGCAAGTTTTGGAGATATATTGATAGCTCCGGGTTGCGACCGCTTATTGATTGCTCATACAGGACATCTAATAAAATTGTTGTATCCGCATTCTGTGAGAGATGGCAACCCAAGACAAATACTTTCCATCTACCATTTGGTGAGTTGACAATCACATTAGATGATGTGTCCAACATACTAGGAATTCCAGTGGATGGTAACTCTGTATCTGTTTGCGATGATGAATTGGACAACTCTGTCCTAGCTAGTCAGTGCCATGATTTATTTGTCACTGCATTAGGAGTTACAGATGATGAGGCACGAGCTGAGATGGGTCGCTTCAGTGGTAGTGCGGTTACACTTGAATGGTTAAGGCAGAGATTTCACAAAGTATCGGATGATGAGTCTCCTGAATTTATAGAGCGTGCAGCAAGGGTTACCTATTATATCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
Mito_carr

Protein Analysis

365

Amino Acids

40.89

Weight (kDa)

8.85

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 243 - 342 2.2e-17 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 896
AccB7I CCANNNNNTGG 1 cut(s) 854
AccII CGCG 2 cut(s) 166, 297
AccIII TCCGGA 1 cut(s) 520
AciI CCGC 8 cut(s) 155, 164, 295, 297, 366, 701, 747, 992
AclWI GGATC 4 cut(s) 497, 510, 521, 534
AcoI YGGCCR 2 cut(s) 122, 283
AcsI RAATTY 3 cut(s) 638, 843, 1052
AcuI CTGAAG 2 cut(s) 411, 964
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 4 cut(s) 167, 426, 512, 854
AgsI TTSAA 4 cut(s) 499, 610, 629, 1004
AjiI CACGTC 1 cut(s) 306
AjnI CCWGG 2 cut(s) 150, 511
AluBI AGCT 6 cut(s) 460, 563, 625, 687, 905, 965
AluI AGCT 6 cut(s) 460, 563, 625, 687, 905, 965
Alw26I GTCTC 4 cut(s) 188, 280, 480, 1050
AlwI GGATC 4 cut(s) 497, 510, 521, 534
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 3 cut(s) 122, 283, 410
ApeKI GCWGC 3 cut(s) 563, 566, 1067
ApoI RAATTY 3 cut(s) 638, 843, 1052
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 170, 300, 411, 518
AsuC2I CCSGG 2 cut(s) 282, 691
AsuHPI GGTGA 1 cut(s) 812
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 3 cut(s) 170, 300, 518
AxyI CCTNAGG 1 cut(s) 599
BalI TGGCCA 1 cut(s) 124
BamHI GGATCC 2 cut(s) 502, 526
BauI CACGAG 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 3 cut(s) 550, 553, 1079
BccI CCATC 5 cut(s) 655, 756, 795, 848, 964
BceAI ACGGC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BciT130I CCWGG 2 cut(s) 152, 513
BciVI GTATCC 2 cut(s) 364, 754
BcnI CCSGG 2 cut(s) 282, 691
BcoDI GTCTC 4 cut(s) 188, 280, 480, 1050
BfaI CTAG 6 cut(s) 38, 399, 461, 839, 902, 906
BfmI CTRYAG 1 cut(s) 444
BfuI GTATCC 2 cut(s) 364, 754
BisI GCNGC 3 cut(s) 564, 567, 1068
BlsI GCNGC 3 cut(s) 565, 568, 1069
Bme1390I CCNGG 4 cut(s) 152, 282, 513, 691
Bme18I GGWCC 3 cut(s) 170, 300, 518
BmgBI CACGTC 1 cut(s) 306
BmgT120I GGNCC 4 cut(s) 170, 300, 411, 518
BmiI GGNNCC 4 cut(s) 159, 301, 504, 528
BmrFI CCNGG 4 cut(s) 152, 282, 513, 691
BmsI GCATC 2 cut(s) 376, 442
BpiI GAAGAC 1 cut(s) 300
Bpu10I CCTNAGC 2 cut(s) 233, 539
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 282, 691
BsaJI CCNNGG 3 cut(s) 280, 295, 530
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 426, 512, 854
Bse1I ACTGG 3 cut(s) 118, 334, 848
Bse21I CCTNAGG 1 cut(s) 599
BseAI TCCGGA 1 cut(s) 520
BseBI CCWGG 2 cut(s) 152, 513
BseDI CCNNGG 3 cut(s) 280, 295, 530
BseGI GGATG 4 cut(s) 391, 647, 859, 1042
BseLI CCNNNNNNNGG 4 cut(s) 167, 426, 512, 854
BseMII CTCAG 2 cut(s) 247, 957
BseNI ACTGG 3 cut(s) 118, 334, 848
BseXI GCAGC 3 cut(s) 550, 553, 1079
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 2 cut(s) 166, 297
Bsh1285I CGRYCG 1 cut(s) 701
BshFI GGCC 3 cut(s) 124, 285, 412
BsiEI CGRYCG 1 cut(s) 701
BsiSI CCGG 3 cut(s) 282, 521, 690
BslI CCNNNNNNNGG 4 cut(s) 167, 426, 512, 854
BsmAI GTCTC 4 cut(s) 188, 280, 480, 1050
BsmBI CGTCTC 2 cut(s) 188, 280
BsmI GAATGC 1 cut(s) 749
BsnI GGCC 3 cut(s) 124, 285, 412
Bsp119I TTCGAA 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 2 cut(s) 502, 526
BspACI CCGC 8 cut(s) 155, 164, 295, 297, 366, 701, 747, 992
BspANI GGCC 3 cut(s) 124, 285, 412
BspCNI CTCAG 2 cut(s) 246, 958
BspEI TCCGGA 1 cut(s) 520
BspFNI CGCG 2 cut(s) 166, 297
BspLI GGNNCC 4 cut(s) 159, 301, 504, 528
BspPI GGATC 4 cut(s) 497, 510, 521, 534
BspT104I TTCGAA 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 3 cut(s) 118, 334, 848
BssECI CCNNGG 3 cut(s) 280, 295, 530
BssMI GATC 2 cut(s) 502, 526
BssSI CACGAG 1 cut(s) 960
Bst2BI CACGAG 1 cut(s) 960
Bst2UI CCWGG 2 cut(s) 152, 513
Bst4CI ACNGT 1 cut(s) 376
BstAUI TGTACA 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 1065
BstDEI CTNAG 4 cut(s) 233, 539, 599, 966
BstDSI CCRYGG 1 cut(s) 295
BstEII GGTNACC 1 cut(s) 1075
BstENI CCTNNNNNAGG 1 cut(s) 424
BstF5I GGATG 4 cut(s) 391, 647, 859, 1042
BstFNI CGCG 2 cut(s) 166, 297
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 2 cut(s) 505, 529
BstMAI GTCTC 4 cut(s) 188, 280, 480, 1050
BstMBI GATC 2 cut(s) 502, 526
BstMCI CGRYCG 1 cut(s) 701
BstNI CCWGG 2 cut(s) 152, 513
BstPI GGTNACC 1 cut(s) 1075
BstSCI CCNGG 4 cut(s) 150, 280, 511, 689
BstSFI CTRYAG 1 cut(s) 444
BstUI CGCG 2 cut(s) 166, 297
BstV1I GCAGC 3 cut(s) 550, 553, 1079
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 2 cut(s) 502, 526
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 2 cut(s) 502, 526
Bsu36I CCTNAGG 1 cut(s) 599
BsuI GTATCC 2 cut(s) 364, 754
BsuRI GGCC 3 cut(s) 124, 285, 412
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 891
BtrI CACGTC 1 cut(s) 306
BtsCI GGATG 4 cut(s) 391, 647, 859, 1042
BtsI GCAGTG 1 cut(s) 930
BtsIMutI CAGTG 6 cut(s) 125, 323, 855, 917, 930, 988
Cac8I GCNNGC 1 cut(s) 1065
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 4 cut(s) 170, 300, 411, 518
Cfr42I CCGCGG 1 cut(s) 298
Csp6I GTAC 1 cut(s) 17
CviAII CATG 3 cut(s) 557, 633, 917
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 4 cut(s) 233, 539, 599, 966
DpnI GATC 2 cut(s) 504, 528
DpnII GATC 2 cut(s) 502, 526
DrdI GACNNNNNNGTC 1 cut(s) 896
DseDI GACNNNNNNGTC 1 cut(s) 896
EaeI YGGCCR 2 cut(s) 122, 283
EciI GGCGGA 1 cut(s) 170
Eco47I GGWCC 3 cut(s) 170, 300, 518
Eco57I CTGAAG 2 cut(s) 411, 964
Eco81I CCTNAGG 1 cut(s) 599
Eco91I GGTNACC 1 cut(s) 1075
EcoNI CCTNNNNNAGG 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 1075
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 2 cut(s) 150, 511
Esp3I CGTCTC 2 cut(s) 188, 280
FaeI CATG 3 cut(s) 560, 636, 920
FatI CATG 3 cut(s) 556, 632, 916
FauI CCCGC 2 cut(s) 290, 359
FauNDI CATATG 1 cut(s) 381
Fnu4HI GCNGC 3 cut(s) 564, 567, 1068
FokI GGATG 4 cut(s) 398, 634, 866, 1049
Fsp4HI GCNGC 3 cut(s) 564, 567, 1068
FspBI CTAG 6 cut(s) 38, 399, 461, 839, 902, 906
GlaI GCGC 1 cut(s) 79
GluI GCNGC 3 cut(s) 564, 567, 1068
HaeIII GGCC 3 cut(s) 124, 285, 412
HapII CCGG 3 cut(s) 282, 521, 690
HhaI GCGC 1 cut(s) 80
Hin1II CATG 3 cut(s) 560, 636, 920
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 4 cut(s) 147, 464, 475, 1043
HpaII CCGG 3 cut(s) 282, 521, 690
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 4 cut(s) 173, 303, 593, 807
Hpy188I TCNGA 2 cut(s) 531, 1036
Hpy188III TCNNGA 4 cut(s) 38, 66, 521, 1049
Hpy8I GTNNAC 4 cut(s) 173, 303, 593, 807
Hpy99I CGWCG 2 cut(s) 112, 360
HpyAV CCTTC 3 cut(s) 263, 386, 493
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 3 cut(s) 181, 305, 589
HpyCH4V TGCA 6 cut(s) 11, 351, 455, 663, 935, 1067
HpyF3I CTNAG 4 cut(s) 233, 539, 599, 966
HpySE526I ACGT 3 cut(s) 181, 305, 589
Hsp92II CATG 3 cut(s) 560, 636, 920
HspAI GCGC 1 cut(s) 78
Kpn2I TCCGGA 1 cut(s) 520
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 2 cut(s) 502, 526
LmnI GCTCC 2 cut(s) 157, 692
Lsp1109I GCAGC 3 cut(s) 550, 553, 1079
LweI GCATC 2 cut(s) 376, 442
MaeI CTAG 6 cut(s) 38, 399, 461, 839, 902, 906
MaeII ACGT 3 cut(s) 181, 305, 589
MaeIII GTNAC 5 cut(s) 857, 928, 943, 994, 1075
MalI GATC 2 cut(s) 504, 528
MboI GATC 2 cut(s) 502, 526
MboII GAAGA 2 cut(s) 216, 300
MflI RGATCY 2 cut(s) 502, 526
MlsI TGGCCA 1 cut(s) 124
MluCI AATT 5 cut(s) 638, 735, 843, 884, 1052
MluNI TGGCCA 1 cut(s) 124
MlyI GAGTC 3 cut(s) 141, 469, 1052
MmeI TCCRAC 2 cut(s) 224, 855
Mox20I TGGCCA 1 cut(s) 124
MroI TCCGGA 1 cut(s) 520
MscI TGGCCA 1 cut(s) 124
MseI TTAA 1 cut(s) 1010
MslI CAYNNNNRTG 2 cut(s) 384, 1003
Msp20I TGGCCA 1 cut(s) 124
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 3 cut(s) 282, 521, 690
MspR9I CCNGG 4 cut(s) 152, 282, 513, 691
Mva1269I GAATGC 1 cut(s) 749
MvaI CCWGG 2 cut(s) 152, 513
MvnI CGCG 2 cut(s) 166, 297
NciI CCSGG 2 cut(s) 282, 691
NdeI CATATG 1 cut(s) 381
NdeII GATC 2 cut(s) 502, 526
NlaIII CATG 3 cut(s) 560, 636, 920
NlaIV GGNNCC 4 cut(s) 159, 301, 504, 528
NmuCI GTSAC 1 cut(s) 928
NspV TTCGAA 1 cut(s) 73
PctI GAATGC 1 cut(s) 749
PfeI GAWTC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 854
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 3 cut(s) 565, 568, 1069
PleI GAGTC 3 cut(s) 141, 469, 1051
PpsI GAGTC 3 cut(s) 141, 469, 1051
Psp6I CCWGG 2 cut(s) 150, 511
PspEI GGTNACC 1 cut(s) 1075
PspGI CCWGG 2 cut(s) 150, 511
PspN4I GGNNCC 4 cut(s) 159, 301, 504, 528
PspPI GGNCC 4 cut(s) 170, 300, 411, 518
PsuI RGATCY 2 cut(s) 502, 526
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 384, 1003
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 1 cut(s) 1010
SatI GCNGC 3 cut(s) 564, 567, 1068
Sau3AI GATC 2 cut(s) 502, 526
Sau96I GGNCC 4 cut(s) 170, 300, 411, 518
SchI GAGTC 3 cut(s) 141, 469, 1052
ScrFI CCNGG 4 cut(s) 152, 282, 513, 691
SfaNI GCATC 2 cut(s) 376, 442
SfcI CTRYAG 1 cut(s) 444
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 3 cut(s) 170, 300, 518
SmiMI CAYNNNNRTG 2 cut(s) 384, 1003
Sse9I AATT 5 cut(s) 638, 735, 843, 884, 1052
SsiI CCGC 8 cut(s) 155, 164, 295, 297, 366, 701, 747, 992
SspMI CTAG 6 cut(s) 38, 399, 461, 839, 902, 906
StyD4I CCNGG 4 cut(s) 150, 280, 511, 689
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 3 cut(s) 184, 308, 592
TaqI TCGA 3 cut(s) 67, 73, 422
TaqII GACCGA 1 cut(s) 176
TasI AATT 5 cut(s) 638, 735, 843, 884, 1052
TatI WGTACW 1 cut(s) 16
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 1010
Tru9I TTAA 1 cut(s) 1010
TscAI CASTG 6 cut(s) 125, 330, 855, 917, 937, 988
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 3 cut(s) 563, 566, 1067
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 1 cut(s) 897
TspGWI ACGGA 6 cut(s) 85, 96, 262, 373, 448, 640
TspRI CASTG 6 cut(s) 125, 330, 855, 917, 937, 988
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 3 cut(s) 170, 300, 518
XagI CCTNNNNNAGG 1 cut(s) 424
XapI RAATTY 3 cut(s) 638, 843, 1052
XbaI TCTAGA 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 2 cut(s) 148, 794
XspI CTAG 6 cut(s) 38, 399, 461, 839, 902, 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.