Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002231

Physical Location & Seq

Genomic Coordinates Reverse (-)

5725260 .. 5740886

Gene Structure 15627 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1081300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3165 bp

ATGAATAATGCAGTGGAGGAGGAGGTGGAAGTTGAACCTCCACTTGCGGTGCTATTGGCTCCAAAACGCGGGAAAACAACCAAGACCAAGCGAAAATCCCAACCAGCAACAACATCCAATCAGCAGAACCCAGAGCCGGAGCCAGTTCGAATCGAGCCGGAGCCTGTTCGAATTGAGCCGGAGCCGGTTGGAGTTGAGCCGGAGGGAGTTCGAATTGAGGCGGAGCCAGTTCGAATTGGGGAAGAAGGCTCGAGGAATGGGTCTACGGTTAGAGTTTCGATGTTCGACGACTCGGTGGAGAATCACTTCAGAGTTATGGACACCATTGCCAAACTCTGCGGCGAGGCGGAGGAGGATCGCGGCGTCGAAGACGGCGAGATTCAACCCTTATCGTCCTCAATTACTTTTCTAAGGGAATGGGCAGATTTTAAGTACGAACCTCGAGATGTTCGATTTGCTTGTGGAGTTGGAAGTCCTGAGGGGGAGGATGTCGTCGGTGGGATAAACTTGCGGCAATTTTCTTCTGCAACAGTTCCTAAGAACGAGGCACTTTCTGGGGTCGCACAATCTCCGGAGTTGAGCCAAGACTTTTTGATGTATGTTGGAGGGCCTGTTTGGGCGTTAGATTGGTGTCCTAGAGTTCATCAAAGTAGCGATGGTCATCCAAAATGCGAGTTTATTGCCGTAGCTGCTCATCCTCCTGGATCTTCTTATCACAAATTAGGCGAGCCACTGACTGGGAGAGGTGCCATTCAGATATGGTGTCTTTTAAATGCTGGTGTAAATAAGGAAGACGAACATTCAATAGCGGAAAAGCAAAAGCAGGGCCGTAAGAACAGTGGAGCTAGACAGGAAAATTCAACTGAACCAAAGAAGCCTTTAGGGAGGCCTAGAAAGAAGCCAATAGAAGAAAAATCAACTGAACCAAAGAGGCCTAGAGGAAGACCTAGAAAGAAGCCAATTGAAGAATCTGCTGACAAAGAGGCCACAGAAGAAAAATCAACTCGACCAAAGAGGCCTAGAGGAAGACCTAAAAAGTATGCAGTAGAAGATTATGTTGACAACTTGGATGAGAGCAATGATTATGTTGAGGTTCTTGCCATCGAATATCCAGAGGGATCACTAGAGTTGCCTTCTATGGATTGTGTTCCTGCAAATACTCATGTGCATGTTGTACAAGAAGATCATGGTAAAAAACGAAAGAGTTACAAACATGCAGCATCTGCATCTGATCCAGCTAGCAAATCACATGTTCAGAGGAGAAAATTGAATAATATGGAAAGTGCAGGGAGTAATAACAGTTATGCATGTCCACCGTTGTCAAATCAAGATGGGGAGAAAGGATCATTTGTTTCAGATCATCATACACAGCAGAATTATGGACAGGATCCTCATAACAGTATTACATGTCCACCGTTGTTGAATCAAGATGGGGAGAAAGGACCACTTGTTGATCATCATACACATCAGAATTCTGGACAGGATCCTCTTAATAGTTGTACATCCCCACCACTGTTGAATCAAGATGGGGAGAAGGGACCACCTGTTTCAGATCATCACACACAGCAGAATTCTGGACATGATCCTCAAACAAGCAAAAATGTTCAAAATAATGGTTACTCTGAAACTGGCTCAACCAGGTGTTCAGTTCCGACAGATGTAGCATTGCCACGAATTGTATTATGCCTGGCTCACCATGGAAAGGTTGCTTGGGATGTGAAATGGCGTCCTCTTAATGAACGTGATTCCAAAAGCAAGTATCGGATGGGCTATCTTGCTGTGCTGTCAGGAAATGGGTCTCTGGAAGTGTGGGATGTTCCTCTTCCTCGTGCAATTGAAGTTATTTATTCTTCTTCATGTGGTGAGGGCACTGATCCTCGCTTTGTAAAATTAGCACCAGTTTTCAGATGCTCAATGTTGAAGTGTGGTGGTGAAAAGAGCATCCCTCTTACTGTGGAATGGTCACCTTCACCACCTTATGATTACCTACTTGTTGGATGCCATGATGGAACGGTTGCTTTATGGAAGTTTTCAGCAAGTAATGCATCTCAAGATACAAGGCCTTTACTTTGCTTCAGTGCAGATACAAATCCAATTAGGGCACTTGCTTGGGCCCCGGCTGAAAGCAATTCAGAGGGTGCCCATCTTATAGCTACTGGTGGACATGGAGGCCTAAAATTTTGGGACCTGCGTGATCCATTCCGTCCGTTGTGGGACCTCGATCATTTACCAAGGTTCATATATAGTCTGAATTGGCTTCCAGATCCAAGGTGTGTTCTTTTATCCTTTGATGATGGAACAATGAAAGCTATCAGCTTGGTGAAGGCTGCATCCGATGACCCTGTAACAGGAAGGGTTGGGACAAAACAACCTGGCCTGCATAATCTCAGCTGTTTGCCTTTTGCTATCTGGAGTGTTCATGCCTCGCAATTAACAGGTATGGTTGCATATTGTGGTGCAGACGGAACTGTTCTTCGCTTCCAGCTTACAACTAAATCTCTGGAGAAAGATCCTCGACGAAACCGAGCCCCACATTTTCTGTGTGTGTCATTGACAATGGAGGAATCTGCTGTAATCATCAATACTCCAGTATCAAATTCCCCTTTCCCACTGAAGGTAGCACGTAACAATGCCGAGTCAAACAAAGTGAAAACTACTAATGATAAGATGGCAAAAGATAACACATCTGAAGATCAAAGTTTAGCTCTTTGTTACGGTGATGATCCAGGTATTGAATTGGAAGGTGGGGGAGAAGTGGCACCTCTCAAAAGCAAAAAAACACAGAAATCCAAGTCCAGTACGAAAAAGCCAGATGATGACCAGGCATTGGTATGCATAGATGAAGAGCCGACTGCTGCACAGGAAGAAGAAACTGGGGTGGCTTCTCTCGACGGCAAAAAAACAAAGAAATCAAAGTCCAGTAAGAAAAATGCGGATGATGACCAAGCATTGGTATGCATGGATGAAGAGCCGACTAATCGAGAAGAAGAAACCGTGAAAGAGCCAGAAGTTTTTCCTGACAAGACAGTAGCAATGCATAGAGTGAGATGGAACATGAACAAGGGTAGTGAGAGATGGCTGTGTTATGGAGGGGCAGCCGGAATTATTCGGTGTCAAGAGATAGTTTTGTCCGACGTCGATAGGGCGTGGGCAGCAAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AT_hook

Protein Analysis

1055

Amino Acids

116.04

Weight (kDa)

5.91

Isoelectric Point (pI)

47.66

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002231)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19485 AT1G19485
fragaria_vesca	FvH4_6g18260 FvH4_6g18260 FvH4_6g18260 FvH4_6g18260 FvH4_7g02500
malus_domestica	MD09G1081300.v1.1 MD12G1022600.v1.1
prunus_persica	Prupe.7G114200_v2.0.a1 Prupe.7G114200_v2.0.a1
pyrus_communis	pycom09g00800 pycom12g02090
rosa_chinensis	RchiOBHm_Chr3g0472691 RchiOBHm_Chr3g0472701 RchiOBHm_Chr4g0409331
rosa_laevigata	RLG00000008568 RLG00000016132 RLG00000024063
rosa_multiflora	Rmu_co8285783.1_g000001 Rmu_sc0001616.1_g000052 Rmu_sc0001616.1_g000057 Rmu_sc0001851.1_g000016 Rmu_sc0003510.1_g000010 Rmu_sc0003510.1_g000011 Rmu_sc0003510.1_g000012 Rmu_sc0034965.1_g000001
rosa_roxburghii	Rroxscaffold_5G00353840 Rroxscaffold_6G00408710
rosa_rugosa	Rorug03G0127200 Rorug04G0092400 Rorug04G0092500
rosa_samantha	Rh3AG179900 Rh3BG205800 Rh3CG202900 Rh3DG169900 Rh4AG155600 Rh4BG153800 Rh4CG164600 Rh4DG148500
rosa_wichuraiana	Rw3G016270 Rw4G012710 Rw4G012730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3138
Acc36I	ACCTGC	1 cut(s)	2196
AccB1I	GGYRCC	3 cut(s)	746, 2138, 2758
AccB7I	CCANNNNNTGG	3 cut(s)	737, 2827, 2950
AccI	GTMKAC	1 cut(s)	263
AccII	CGCG	2 cut(s)	69, 360
AccIII	TCCGGA	1 cut(s)	571
AciI	CCGC	9 cut(s)	47, 69, 221, 339, 347, 360, 511, 809, 2933
AcsI	RAATTY	5 cut(s)	856, 1471, 1570, 2177, 2596
AcuI	CTGAAG	4 cut(s)	292, 2059, 2633, 2709
AcyI	GRCGYC	3 cut(s)	363, 1726, 3135
AfaI	GTAC	4 cut(s)	434, 1176, 1501, 2800
AfiI	CCNNNNNNNGG	8 cut(s)	68, 136, 737, 1702, 2348, 2614, 2827, 2950
AflIII	ACRYGT	2 cut(s)	1249, 1406
AjnI	CCWGG	6 cut(s)	700, 1637, 1686, 2371, 2725, 2820
AjuI	GAANNNNNNNTTGG	2 cut(s)	1693, 1725
AluBI	AGCT	9 cut(s)	689, 845, 1238, 2153, 2309, 2316, 2391, 2485, 2705
AluI	AGCT	9 cut(s)	689, 845, 1238, 2153, 2309, 2316, 2391, 2485, 2705
Alw26I	GTCTC	1 cut(s)	1803
AlwNI	CAGNNNCTG	1 cut(s)	1223
Ama87I	CYCGRG	2 cut(s)	250, 441
Aor13HI	TCCGGA	1 cut(s)	571
ApaI	GGGCCC	1 cut(s)	2116
ApeKI	GCWGC	6 cut(s)	689, 1217, 2327, 2855, 3095, 3152
ApoI	RAATTY	5 cut(s)	856, 1471, 1570, 2177, 2596
Asp700I	GAANNNNTTC	2 cut(s)	305, 3012
AspS9I	GGNCC	8 cut(s)	608, 826, 1442, 1538, 2112, 2113, 2185, 2215
AsuC2I	CCSGG	1 cut(s)	2117
AsuHPI	GGTGA	7 cut(s)	1685, 1874, 1943, 1956, 1962, 2332, 2729
AsuII	TTCGAA	4 cut(s)	148, 169, 211, 232
AsuNHI	GCTAGC	1 cut(s)	1238
AvaI	CYCGRG	2 cut(s)	250, 441
AvaII	GGWCC	4 cut(s)	1442, 1538, 2185, 2215
AxyI	CCTNAGG	1 cut(s)	477
BaeGI	GKGCMC	4 cut(s)	1871, 2104, 2116, 2143
BaeI	ACNNNNGTAYC	2 cut(s)	2574, 2607
BamHI	GGATCC	2 cut(s)	1387, 1483
BanI	GGYRCC	3 cut(s)	746, 2138, 2758
BanII	GRGCYC	2 cut(s)	2116, 2530
BauI	CACGAG	1 cut(s)	1827
BbsI	GAAGAC	4 cut(s)	375, 798, 949, 1033
BbvI	GCAGC	5 cut(s)	676, 1229, 2314, 2842, 3107
BceAI	ACGGC	4 cut(s)	388, 668, 813, 2908
BcgI	CGANNNNNNTGC	2 cut(s)	662, 696
BciT130I	CCWGG	6 cut(s)	702, 1639, 1688, 2373, 2727, 2822
BclI	TGATCA	1 cut(s)	1453
BcnI	CCSGG	1 cut(s)	2117
BcoDI	GTCTC	1 cut(s)	1803
BfaI	CTAG	8 cut(s)	636, 846, 891, 936, 948, 1020, 1124, 1239
BfuAI	ACCTGC	1 cut(s)	2196
BisI	GCNGC	9 cut(s)	340, 361, 512, 690, 1218, 2328, 2856, 3096, 3153
BlsI	GCNGC	9 cut(s)	341, 362, 513, 691, 1219, 2329, 2857, 3097, 3154
Bme1390I	CCNGG	7 cut(s)	702, 1639, 1688, 2117, 2373, 2727, 2822
Bme18I	GGWCC	4 cut(s)	1442, 1538, 2185, 2215
BmeT110I	CYCGRG	2 cut(s)	250, 441
BmgT120I	GGNCC	8 cut(s)	608, 826, 1442, 1538, 2112, 2113, 2185, 2215
BmrFI	CCNGG	7 cut(s)	702, 1639, 1688, 2117, 2373, 2727, 2822
BmrI	ACTGGG	2 cut(s)	747, 2883
BmsI	GCATC	7 cut(s)	1229, 1235, 1898, 1950, 1988, 2054, 2339
BmtI	GCTAGC	1 cut(s)	1242
BmuI	ACTGGG	2 cut(s)	747, 2883
BpiI	GAAGAC	4 cut(s)	375, 798, 949, 1033
BplI	GAGNNNNNCTC	2 cut(s)	1930, 1962
BpmI	CTGGAG	3 cut(s)	2431, 2522, 2571
Bpu14I	TTCGAA	4 cut(s)	148, 169, 211, 232
BpuEI	CTTGAG	1 cut(s)	2034
BpuMI	CCSGG	1 cut(s)	2117
BsaAI	YACGTR	1 cut(s)	2624
BsaBI	GATNNNNATC	2 cut(s)	660, 2088
BsaHI	GRCGYC	3 cut(s)	363, 1726, 3135
BsaI	GGTCTC	1 cut(s)	1803
BsaJI	CCNNGG	4 cut(s)	1696, 2115, 2231, 2267
BsaWI	WCCGGW	1 cut(s)	571
BsaXI	ACNNNNNCTCC	2 cut(s)	597, 627
Bsc4I	CCNNNNNNNGG	8 cut(s)	68, 136, 737, 1702, 2348, 2614, 2827, 2950
Bse118I	RCCGGY	1 cut(s)	184
Bse21I	CCTNAGG	1 cut(s)	477
Bse3DI	GCAATG	4 cut(s)	324, 1084, 1664, 3039
Bse8I	GATNNNNATC	2 cut(s)	660, 2088
BseAI	TCCGGA	1 cut(s)	571
BseBI	CCWGG	6 cut(s)	702, 1639, 1688, 2373, 2727, 2822
BseDI	CCNNGG	4 cut(s)	1696, 2115, 2231, 2267
BseJI	GATNNNNATC	2 cut(s)	660, 2088
BseLI	CCNNNNNNNGG	8 cut(s)	68, 136, 737, 1702, 2348, 2614, 2827, 2950
BseMI	GCAATG	4 cut(s)	324, 1084, 1664, 3039
BseMII	CTCAG	2 cut(s)	468, 2401
BseRI	GAGGAG	4 cut(s)	32, 35, 365, 1273
BseSI	GKGCMC	4 cut(s)	1871, 2104, 2116, 2143
BseXI	GCAGC	5 cut(s)	676, 1229, 2314, 2842, 3107
BsgI	GTGCAG	4 cut(s)	1305, 2100, 2478, 2841
Bsh1236I	CGCG	2 cut(s)	69, 360
BshNI	GGYRCC	3 cut(s)	746, 2138, 2758
BsiHKCI	CYCGRG	2 cut(s)	250, 441
BsiSI	CCGG	8 cut(s)	137, 158, 179, 185, 200, 572, 2117, 3099
BslFI	GGGAC	4 cut(s)	1551, 2198, 2228, 2374
BslI	CCNNNNNNNGG	8 cut(s)	68, 136, 737, 1702, 2348, 2614, 2827, 2950
BsmAI	GTCTC	1 cut(s)	1803
BsmFI	GGGAC	4 cut(s)	1551, 2198, 2228, 2374
Bso31I	GGTCTC	1 cut(s)	1803
BsoBI	CYCGRG	2 cut(s)	250, 441
Bsp119I	TTCGAA	4 cut(s)	148, 169, 211, 232
Bsp120I	GGGCCC	1 cut(s)	2112
Bsp1286I	GDGCHC	5 cut(s)	1871, 2104, 2116, 2143, 2530
Bsp13I	TCCGGA	1 cut(s)	571
Bsp1407I	TGTACA	2 cut(s)	1174, 1499
Bsp19I	CCATGG	1 cut(s)	1696
BspACI	CCGC	9 cut(s)	47, 69, 221, 339, 347, 360, 511, 809, 2933
BspCNI	CTCAG	2 cut(s)	469, 2400
BspEI	TCCGGA	1 cut(s)	571
BspFNI	CGCG	2 cut(s)	69, 360
BspMI	ACCTGC	1 cut(s)	2196
BspOI	GCTAGC	1 cut(s)	1242
BspQI	GCTCTTC	2 cut(s)	2838, 2961
BspT104I	TTCGAA	4 cut(s)	148, 169, 211, 232
BspT107I	GGYRCC	3 cut(s)	746, 2138, 2758
BspTNI	GGTCTC	1 cut(s)	1803
BsrDI	GCAATG	4 cut(s)	324, 1084, 1664, 3039
BsrFI	RCCGGY	1 cut(s)	184
BsrGI	TGTACA	2 cut(s)	1174, 1499
BssAI	RCCGGY	1 cut(s)	184
BssECI	CCNNGG	4 cut(s)	1696, 2115, 2231, 2267
BssNI	GRCGYC	3 cut(s)	363, 1726, 3135
BssSI	CACGAG	1 cut(s)	1827
BssT1I	CCWWGG	3 cut(s)	1696, 2231, 2267
Bst2BI	CACGAG	1 cut(s)	1827
Bst2UI	CCWGG	6 cut(s)	702, 1639, 1688, 2373, 2727, 2822
Bst6I	CTCTTC	3 cut(s)	1827, 2838, 2961
BstACI	GRCGYC	3 cut(s)	363, 1726, 3135
BstAPI	GCANNNNNTGC	2 cut(s)	1223, 2042
BstAUI	TGTACA	2 cut(s)	1174, 1499
BstBAI	YACGTR	1 cut(s)	2624
BstBI	TTCGAA	4 cut(s)	148, 169, 211, 232
BstC8I	GCNNGC	3 cut(s)	728, 1240, 2378
BstDEI	CTNAG	4 cut(s)	410, 477, 537, 2387
BstDSI	CCRYGG	1 cut(s)	1696
BstEII	GGTNACC	1 cut(s)	1962
BstENI	CCTNNNNNAGG	1 cut(s)	2346
BstFNI	CGCG	2 cut(s)	69, 360
BstMAI	GTCTC	1 cut(s)	1803
BstMWI	GCNNNNNNNGC	4 cut(s)	689, 1223, 2042, 3152
BstNI	CCWGG	6 cut(s)	702, 1639, 1688, 2373, 2727, 2822
BstNSI	RCATGY	5 cut(s)	1172, 1217, 1253, 1311, 1410
BstPI	GGTNACC	1 cut(s)	1962
BstSCI	CCNGG	7 cut(s)	700, 1637, 1686, 2115, 2371, 2725, 2820
BstSLI	GKGCMC	4 cut(s)	1871, 2104, 2116, 2143
BstUI	CGCG	2 cut(s)	69, 360
BstV1I	GCAGC	5 cut(s)	676, 1229, 2314, 2842, 3107
BstV2I	GAAGAC	4 cut(s)	375, 798, 949, 1033
BstX2I	RGATCY	5 cut(s)	704, 1387, 1483, 2263, 2509
BstYI	RGATCY	5 cut(s)	704, 1387, 1483, 2263, 2509
Bsu36I	CCTNAGG	1 cut(s)	477
BtgI	CCRYGG	1 cut(s)	1696
BtgZI	GCGATG	1 cut(s)	669
BtsI	GCAGTG	1 cut(s)	18
BtsIMutI	CAGTG	7 cut(s)	18, 731, 844, 1511, 1869, 2083, 2609
BveI	ACCTGC	1 cut(s)	2196
Cac8I	GCNNGC	3 cut(s)	728, 1240, 2378
CaiI	CAGNNNCTG	1 cut(s)	1223
Cfr10I	RCCGGY	1 cut(s)	184
Cfr13I	GGNCC	8 cut(s)	608, 826, 1442, 1538, 2112, 2113, 2185, 2215
CseI	GACGC	2 cut(s)	352, 1715
CsiI	ACCWGGT	1 cut(s)	1637
Csp6I	GTAC	4 cut(s)	433, 1175, 1500, 2799
CspCI	CAANNNNNGTGG	4 cut(s)	1401, 1436, 1497, 1532
CviQI	GTAC	4 cut(s)	433, 1175, 1500, 2799
DdeI	CTNAG	4 cut(s)	410, 477, 537, 2387
DraI	TTTAAA	1 cut(s)	771
Eam1104I	CTCTTC	3 cut(s)	1827, 2838, 2961
EarI	CTCTTC	3 cut(s)	1827, 2838, 2961
EciI	GGCGGA	2 cut(s)	236, 362
Eco130I	CCWWGG	3 cut(s)	1696, 2231, 2267
Eco147I	AGGCCT	5 cut(s)	889, 934, 1018, 2062, 2172
Eco24I	GRGCYC	2 cut(s)	2116, 2530
Eco31I	GGTCTC	1 cut(s)	1803
Eco47I	GGWCC	4 cut(s)	1442, 1538, 2185, 2215
Eco57I	CTGAAG	4 cut(s)	292, 2059, 2633, 2709
Eco81I	CCTNAGG	1 cut(s)	477
Eco88I	CYCGRG	2 cut(s)	250, 441
Eco91I	GGTNACC	1 cut(s)	1962
EcoNI	CCTNNNNNAGG	1 cut(s)	2346
EcoO109I	RGGNCCY	4 cut(s)	608, 2113, 2185, 2215
EcoO65I	GGTNACC	1 cut(s)	1962
EcoRI	GAATTC	2 cut(s)	1471, 1570
EcoRII	CCWGG	6 cut(s)	700, 1637, 1686, 2371, 2725, 2820
EcoT14I	CCWWGG	3 cut(s)	1696, 2231, 2267
EcoT22I	ATGCAT	5 cut(s)	1309, 2047, 2837, 2960, 3039
EcoT38I	GRGCYC	2 cut(s)	2116, 2530
ErhI	CCWWGG	3 cut(s)	1696, 2231, 2267
FalI	AAGNNNNNCTT	2 cut(s)	1431, 1463
FaqI	GGGAC	4 cut(s)	1551, 2198, 2228, 2374
FauI	CCCGC	1 cut(s)	62
FbaI	TGATCA	1 cut(s)	1453
FblI	GTMKAC	1 cut(s)	263
Fnu4HI	GCNGC	9 cut(s)	340, 361, 512, 690, 1218, 2328, 2856, 3096, 3153
FriOI	GRGCYC	2 cut(s)	2116, 2530
Fsp4HI	GCNGC	9 cut(s)	340, 361, 512, 690, 1218, 2328, 2856, 3096, 3153
FspBI	CTAG	8 cut(s)	636, 846, 891, 936, 948, 1020, 1124, 1239
GluI	GCNGC	9 cut(s)	340, 361, 512, 690, 1218, 2328, 2856, 3096, 3153
GsuI	CTGGAG	3 cut(s)	2431, 2522, 2571
HapII	CCGG	8 cut(s)	137, 158, 179, 185, 200, 572, 2117, 3099
HgaI	GACGC	2 cut(s)	352, 1715
Hin1I	GRCGYC	3 cut(s)	363, 1726, 3135
HincII	GTYRAC	1 cut(s)	1060
HindII	GTYRAC	1 cut(s)	1060
HpaII	CCGG	8 cut(s)	137, 158, 179, 185, 200, 572, 2117, 3099
HphI	GGTGA	7 cut(s)	1685, 1874, 1943, 1956, 1962, 2332, 2729
Hpy166II	GTNNAC	5 cut(s)	264, 1060, 1313, 1412, 2162
Hpy8I	GTNNAC	5 cut(s)	264, 1060, 1313, 1412, 2162
Hpy99I	CGWCG	7 cut(s)	290, 368, 497, 2520, 2894, 3137, 3140
HpyAV	CCTTC	8 cut(s)	239, 1143, 1528, 1977, 2317, 2346, 2608, 2735
HpyCH4IV	ACGT	3 cut(s)	1741, 2623, 3135
HpyF10VI	GCNNNNNNNGC	4 cut(s)	689, 1223, 2042, 3152
HpyF3I	CTNAG	4 cut(s)	410, 477, 537, 2387
HpySE526I	ACGT	3 cut(s)	1741, 2623, 3135
Hsp92I	GRCGYC	3 cut(s)	363, 1726, 3135
Kpn2I	TCCGGA	1 cut(s)	571
Ksp22I	TGATCA	1 cut(s)	1453
LguI	GCTCTTC	2 cut(s)	2838, 2961
LmnI	GCTCC	6 cut(s)	64, 139, 160, 181, 223, 842
Lsp1109I	GCAGC	5 cut(s)	676, 1229, 2314, 2842, 3107
LweI	GCATC	7 cut(s)	1229, 1235, 1898, 1950, 1988, 2054, 2339
MabI	ACCWGGT	1 cut(s)	1637
MaeI	CTAG	8 cut(s)	636, 846, 891, 936, 948, 1020, 1124, 1239
MaeII	ACGT	3 cut(s)	1741, 2623, 3135
MaeIII	GTNAC	6 cut(s)	1205, 1616, 1962, 2344, 2624, 2711
MfeI	CAATTG	2 cut(s)	960, 1833
MflI	RGATCY	5 cut(s)	704, 1387, 1483, 2263, 2509
MhlI	GDGCHC	5 cut(s)	1871, 2104, 2116, 2143, 2530
MlyI	GAGTC	2 cut(s)	284, 2645
MmeI	TCCRAC	6 cut(s)	169, 448, 583, 1676, 1975, 3156
Mph1103I	ATGCAT	5 cut(s)	1309, 2047, 2837, 2960, 3039
MroI	TCCGGA	1 cut(s)	571
MroXI	GAANNNNTTC	2 cut(s)	305, 3012
MseI	TTAA	5 cut(s)	429, 770, 1491, 1734, 2432
MslI	CAYNNNNRTG	3 cut(s)	1167, 2830, 2953
MspA1I	CMGCKG	1 cut(s)	2391
MspI	CCGG	8 cut(s)	137, 158, 179, 185, 200, 572, 2117, 3099
MspR9I	CCNGG	7 cut(s)	702, 1639, 1688, 2117, 2373, 2727, 2822
MunI	CAATTG	2 cut(s)	960, 1833
MvaI	CCWGG	6 cut(s)	702, 1639, 1688, 2373, 2727, 2822
MvnI	CGCG	2 cut(s)	69, 360
MwoI	GCNNNNNNNGC	4 cut(s)	689, 1223, 2042, 3152
NciI	CCSGG	1 cut(s)	2117
NcoI	CCATGG	1 cut(s)	1696
NheI	GCTAGC	1 cut(s)	1238
NmeAIII	GCCGAG	1 cut(s)	2659
NmuCI	GTSAC	1 cut(s)	1962
NsiI	ATGCAT	5 cut(s)	1309, 2047, 2837, 2960, 3039
NspI	RCATGY	5 cut(s)	1172, 1217, 1253, 1311, 1410
NspV	TTCGAA	4 cut(s)	148, 169, 211, 232
PaeR7I	CTCGAG	2 cut(s)	250, 441
PceI	AGGCCT	5 cut(s)	889, 934, 1018, 2062, 2172
PciI	ACATGT	2 cut(s)	1249, 1406
PciSI	GCTCTTC	2 cut(s)	2838, 2961
PcsI	WCGNNNNNNNCGW	3 cut(s)	372, 448, 2521
PdmI	GAANNNNTTC	2 cut(s)	305, 3012
PfeI	GAWTC	8 cut(s)	150, 301, 379, 968, 1423, 1519, 1745, 2564
PflMI	CCANNNNNTGG	3 cut(s)	737, 2827, 2950
PfoI	TCCNGGA	1 cut(s)	700
PkrI	GCNGC	9 cut(s)	341, 362, 513, 691, 1219, 2329, 2857, 3097, 3154
PleI	GAGTC	2 cut(s)	284, 2644
PpsI	GAGTC	2 cut(s)	284, 2644
Ppu21I	YACGTR	1 cut(s)	2624
PpuMI	RGGWCCY	2 cut(s)	2185, 2215
PscI	ACATGT	2 cut(s)	1249, 1406
Psp5II	RGGWCCY	2 cut(s)	2185, 2215
Psp6I	CCWGG	6 cut(s)	700, 1637, 1686, 2371, 2725, 2820
PspEI	GGTNACC	1 cut(s)	1962
PspGI	CCWGG	6 cut(s)	700, 1637, 1686, 2371, 2725, 2820
PspOMI	GGGCCC	1 cut(s)	2112
PspPI	GGNCC	8 cut(s)	608, 826, 1442, 1538, 2112, 2113, 2185, 2215
PspPPI	RGGWCCY	2 cut(s)	2185, 2215
PspXI	VCTCGAGB	1 cut(s)	250
PstNI	CAGNNNCTG	1 cut(s)	1223
PsuI	RGATCY	5 cut(s)	704, 1387, 1483, 2263, 2509
PvuII	CAGCTG	1 cut(s)	2391
RsaI	GTAC	4 cut(s)	434, 1176, 1501, 2800
RsaNI	GTAC	4 cut(s)	433, 1175, 1500, 2799
RseI	CAYNNNNRTG	3 cut(s)	1167, 2830, 2953
SapI	GCTCTTC	2 cut(s)	2838, 2961
SaqAI	TTAA	5 cut(s)	429, 770, 1491, 1734, 2432
SatI	GCNGC	9 cut(s)	340, 361, 512, 690, 1218, 2328, 2856, 3096, 3153
Sau96I	GGNCC	8 cut(s)	608, 826, 1442, 1538, 2112, 2113, 2185, 2215
SchI	GAGTC	2 cut(s)	284, 2645
ScrFI	CCNGG	7 cut(s)	702, 1639, 1688, 2117, 2373, 2727, 2822
SduI	GDGCHC	5 cut(s)	1871, 2104, 2116, 2143, 2530
SexAI	ACCWGGT	1 cut(s)	1637
SfaNI	GCATC	7 cut(s)	1229, 1235, 1898, 1950, 1988, 2054, 2339
Sfr274I	CTCGAG	2 cut(s)	250, 441
SfuI	TTCGAA	4 cut(s)	148, 169, 211, 232
SinI	GGWCC	4 cut(s)	1442, 1538, 2185, 2215
SlaI	CTCGAG	2 cut(s)	250, 441
SmiMI	CAYNNNNRTG	3 cut(s)	1167, 2830, 2953
SmlI	CTYRAG	3 cut(s)	250, 441, 2049
SmoI	CTYRAG	3 cut(s)	250, 441, 2049
SseBI	AGGCCT	5 cut(s)	889, 934, 1018, 2062, 2172
SsiI	CCGC	9 cut(s)	47, 69, 221, 339, 347, 360, 511, 809, 2933
SspMI	CTAG	8 cut(s)	636, 846, 891, 936, 948, 1020, 1124, 1239
StuI	AGGCCT	5 cut(s)	889, 934, 1018, 2062, 2172
StyD4I	CCNGG	7 cut(s)	700, 1637, 1686, 2115, 2371, 2725, 2820
StyI	CCWWGG	3 cut(s)	1696, 2231, 2267
TaiI	ACGT	3 cut(s)	1744, 2626, 3138
TatI	WGTACW	2 cut(s)	1174, 1499
TauI	GCSGC	3 cut(s)	342, 363, 514
TfiI	GAWTC	8 cut(s)	150, 301, 379, 968, 1423, 1519, 1745, 2564
Tru1I	TTAA	5 cut(s)	429, 770, 1491, 1734, 2432
Tru9I	TTAA	5 cut(s)	429, 770, 1491, 1734, 2432
TscAI	CASTG	7 cut(s)	18, 738, 844, 1518, 1876, 2083, 2616
TseFI	GTSAC	1 cut(s)	1962
TseI	GCWGC	6 cut(s)	689, 1217, 2327, 2855, 3095, 3152
Tsp45I	GTSAC	1 cut(s)	1962
TspGWI	ACGGA	3 cut(s)	2192, 2196, 2478
TspRI	CASTG	7 cut(s)	18, 738, 844, 1518, 1876, 2083, 2616
Van91I	CCANNNNNTGG	3 cut(s)	737, 2827, 2950
VpaK11BI	GGWCC	4 cut(s)	1442, 1538, 2185, 2215
XagI	CCTNNNNNAGG	1 cut(s)	2346
XapI	RAATTY	5 cut(s)	856, 1471, 1570, 2177, 2596
XceI	RCATGY	5 cut(s)	1172, 1217, 1253, 1311, 1410
XhoI	CTCGAG	2 cut(s)	250, 441
XmiI	GTMKAC	1 cut(s)	263
XmnI	GAANNNNTTC	2 cut(s)	305, 3012
XspI	CTAG	8 cut(s)	636, 846, 891, 936, 948, 1020, 1124, 1239
ZraI	GACGTC	1 cut(s)	3136
Zsp2I	ATGCAT	5 cut(s)	1309, 2047, 2837, 2960, 3039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.