Rmu_sc0034965.1_g000001

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034965.1
OG0002231
Physical Location & Seq
Forward (+)
1 .. 1868
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034965.1_g000001.1.cds

Sequence Viewer

Length: 735 bp
gactatgtgatgtatgttggagggcctgtttgggcattggattggtgtcctagagttcatgaaagtcacgaccgtcatgcaaagtgcgagtttattgccattgctgctcatccccctggatcttcttatcacaagttaggtgagccacttactggcagaggtgccattcagatatggtgtcttctaaatgctagtgtaaatgatgaaggtgttcctataggagacaaaccaaaaggaggaaataaaaggagtggagccacagaaaaatgcactgaacaaaaaaggggaagacccagaaagaagccattagaagaatctgttggcaaagaggccacagaagaaaaactgtctgagccaaaaaggccgagaggaagacctagaaagaagccaatagaagaatctattgacaaagagggcaaagaagagaaatcaactcaaccaaagaggcctagaggaagacctagaaataaatcagtagaagaatctgttgacaacttggatggtctcaccaattgtgatgaggttcttgctattgattatccaaaggaatcatctgcgttgcattctatggattgtcttcctgcaaatactcaaggaaattttgtacaagaatatcatggcaaaaagcaactaagttatgggcagagtcaaccgaagaagcctagaggaagacccagaaagaagccaataaaagaacctgttgacaatttagatgggggcaacaataattttgag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

245

Amino Acids

27.39

Weight (kDa)

9.1

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 152
AclWI GGATC 1 cut(s) 127
AcsI RAATTY 1 cut(s) 598
AfaI GTAC 1 cut(s) 606
AfiI CCNNNNNNNGG 2 cut(s) 152, 236
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
Alw26I GTCTC 2 cut(s) 216, 509
AlwI GGATC 1 cut(s) 127
AoxI GGCC 4 cut(s) 23, 330, 362, 446
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 2 cut(s) 152, 499
BanI GGYRCC 1 cut(s) 161
BbsI GAAGAC 6 cut(s) 173, 295, 379, 463, 569, 676
BbvI GCAGC 1 cut(s) 91
BccI CCATC 2 cut(s) 494, 707
BcgI CGANNNNNNTGC 4 cut(s) 59, 77, 93, 111
BciT130I CCWGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 216, 509
BfaI CTAG 6 cut(s) 51, 192, 378, 450, 462, 663
BfmI CTRYAG 1 cut(s) 216
BglI GCCNNNNNGGC 1 cut(s) 361
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 117
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 163, 256
BmrFI CCNGG 1 cut(s) 117
BpiI GAAGAC 6 cut(s) 173, 295, 379, 463, 569, 676
BpuEI CTTGAG 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 236
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 2 cut(s) 109, 505
BseLI CCNNNNNNNGG 2 cut(s) 152, 236
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 157
BseXI GCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 4 cut(s) 25, 332, 364, 448
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 152, 236
BsmAI GTCTC 2 cut(s) 216, 509
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 4 cut(s) 25, 332, 364, 448
Bso31I GGTCTC 1 cut(s) 509
Bsp1407I TGTACA 1 cut(s) 604
Bsp143I GATC 1 cut(s) 119
BspANI GGCC 4 cut(s) 25, 332, 364, 448
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 163, 256
BspPI GGATC 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 99
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 74, 348
Bst6I CTCTTC 1 cut(s) 417
BstAUI TGTACA 1 cut(s) 604
BstDEI CTNAG 2 cut(s) 351, 632
BstF5I GGATG 2 cut(s) 109, 505
BstKTI GATC 1 cut(s) 122
BstMAI GTCTC 2 cut(s) 216, 509
BstMBI GATC 1 cut(s) 119
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 104, 361
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 6 cut(s) 173, 295, 379, 463, 569, 676
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BsuRI GGCC 4 cut(s) 25, 332, 364, 448
BtsCI GGATG 2 cut(s) 109, 505
BtsIMutI CAGTG 1 cut(s) 270
CciI TCATGA 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 605
CviAII CATG 3 cut(s) 59, 77, 617
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 2 cut(s) 351, 632
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 448
Eco31I GGTCTC 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 115
FaeI CATG 3 cut(s) 62, 80, 620
FaiI YATR 9 cut(s) 6, 15, 60, 78, 175, 218, 569, 618, 639
FatI CATG 3 cut(s) 58, 76, 616
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 2 cut(s) 96, 512
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 6 cut(s) 51, 192, 378, 450, 462, 663
GluI GCNGC 1 cut(s) 105
HaeIII GGCC 4 cut(s) 25, 332, 364, 448
Hin1II CATG 3 cut(s) 62, 80, 620
HincII GTYRAC 3 cut(s) 490, 650, 703
HindII GTYRAC 3 cut(s) 490, 650, 703
HinfI GANTC 5 cut(s) 314, 398, 482, 548, 646
HphI GGTGA 2 cut(s) 152, 499
Hpy166II GTNNAC 3 cut(s) 490, 650, 703
Hpy188I TCNGA 2 cut(s) 171, 352
Hpy188III TCNNGA 2 cut(s) 59, 68
Hpy8I GTNNAC 3 cut(s) 490, 650, 703
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 2 cut(s) 74, 348
HpyCH4V TGCA 4 cut(s) 80, 270, 562, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 361
HpyF3I CTNAG 2 cut(s) 351, 632
Hsp92II CATG 3 cut(s) 62, 80, 620
Kzo9I GATC 1 cut(s) 119
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 8 cut(s) 39, 102, 129, 138, 307, 594, 688, 711
Lsp1109I GCAGC 1 cut(s) 91
MaeI CTAG 6 cut(s) 51, 192, 378, 450, 462, 663
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 121
MboI GATC 1 cut(s) 119
MfeI CAATTG 1 cut(s) 511
MflI RGATCY 1 cut(s) 119
MluCI AATT 4 cut(s) 511, 598, 706, 727
MlyI GAGTC 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 104, 361
NdeII GATC 1 cut(s) 119
NlaIII CATG 3 cut(s) 62, 80, 620
NlaIV GGNNCC 2 cut(s) 163, 256
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 65
PagI TCATGA 1 cut(s) 58
PceI AGGCCT 1 cut(s) 448
PctI GAATGC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 4 cut(s) 314, 398, 482, 548
PflMI CCANNNNNTGG 1 cut(s) 152
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 654
PpsI GAGTC 1 cut(s) 654
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 163, 256
PspPI GGNCC 1 cut(s) 23
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 1 cut(s) 119
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 1 cut(s) 117
SetI ASST 7 cut(s) 142, 163, 211, 379, 463, 525, 700
SfcI CTRYAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
Sse9I AATT 4 cut(s) 511, 598, 706, 727
SseBI AGGCCT 1 cut(s) 448
SspMI CTAG 6 cut(s) 51, 192, 378, 450, 462, 663
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 74, 348
TasI AATT 4 cut(s) 511, 598, 706, 727
TatI WGTACW 1 cut(s) 604
TfiI GAWTC 4 cut(s) 314, 398, 482, 548
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 3 cut(s) 47, 75, 219
TspRI CASTG 1 cut(s) 277
Van91I CCANNNNNTGG 1 cut(s) 152
XapI RAATTY 1 cut(s) 598
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 6 cut(s) 51, 192, 378, 450, 462, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.