RchiOBHm_Chr4g0409331

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002231
Physical Location & Seq
Forward (+)
32737869 .. 32748951
11083 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38036

Sequence Viewer

Length: 3333 bp
ATGAATGAAGAGGAAGTGGAACCTCCACTCGGGGTTCTATTGGGTCGAAAGCGCGGGAAAACGAAGTCCGCCAAGAGAGCAAGCCAAGTAACACCGCACAGCACAGAGCCACTACAAATCGAACCAGAGCCTGTCCCGATCGATGCGGAGCCAATTGCAATTGAGCCAGAGAACTCAAGGAATGGCGCTAGGGTTAGTGTTTCGATGTTCGATGACTCGGTGGAGAATCATTTTAGAGTCATGCACACGATTTCTGAGCTCTGTGGCGACGCCGAAGACGAGCCACTCCCGGTGAGCGACATTCAACGCTTATCATCCTCCACCACTTTCTTGAGGGAATGGGCAGATTTTAAGTATGAACCTAGAGATGTTCGATTCGCTTGCGAAGCAGAAAGCTCTGATGAGGAGGATGTTGTCAGTAGGATAAACTTGCGGCAGTTTTCTTCTGCAACTGTTCCACAGAACGACGCGGGGAACACATCTTCTCCAGAGTTGAGACAAGACTATGTGATGTATGTTGGAGGGCCTGTTTGGGCATTGGATTGGTGTCCTAGAGTTCATGAAAGTCACGACCGTCATGCAAAGTGCGAGTTTATTGCCATTGCTGCTCATCCCCCTGGATCTTCTTATCACAAGTTAGGTGAGCCACTTACTGGCAGAGGTGCCATTCAGATATGGTGTCTTCTAAATGCTAGTGTAAATGATGAAGGTGTTCCTATAGGAGACAAACCAAAAGGAGGAAATAAAAGGAGTGGAGCCACAGAAAAATGCACTGAACAAAAAAGGGGAAGACCCAGAAAGAAGCCATTAGAAGAATCTGTTGGCAAAGAGGCCACAGAAGAAAAACTGTCTGAGCCAAAAAGGCCGAGAGGAAGACCTAGAAAGAAGCCAATAGAAGAATCTATTGACAAAGAGGCCAAAGAAGAGAAATCAACTCAACCAAAGAGGCCTAGAGGAAGACCTAGAAATAAATTAATAGAAGAATCTGTTGACAACTTGGATGGGAGAACCAATTGTGATGAGGTTCTTGCTATTGATTATCCAAAGGAATCATCTGAGTTGCATTCTATGGATTGTCTTCCTGCAAATACTCAAGGAAATTTTGTACAAGAATATCATGGCAAAAAGCAACTAAGCTATGGGCAGAGTCAACCGAAGAAGCCTAGAGGAAGACCCAGAAAGAAGCCAATAAAAGAACCTGTTGACAATTTAGATGGGGGCAACAATAATTTTGAGGTTCTTGCTATTGAATACCAAAAGGAATCACTGGAGTTGCATTCTACGGATTGTCTTCTTGCAAATACTCAAGTGGACGCTGTACAAGAAACAAGTTACAAGCAGACTCGATCAAAGAGGCCTAGAGGAATACATGGAAAGAAGCCAATAGAAGAATCTGTTGAAAACTTGGATGACAGCAGCAATACGGGTGAGGCTCTTGATGATCAATATCCAAAGGAATTATTGGAGTTGGAGGGCAGGGATTGCGCTCCTGAAAATACTCAAGGAGATGAGGTACAAGATGATAGTGGTAAGAAAAGAAAAACGTACAGAAAGACTCGAGCAAAGATTCCTAGAGAAAAACCTGGGGAGAAGCTAGTAGAATATTCTGTTGATAACTTGCATGGGAGCAGCCATTATGCGGAGGCTCTTGGAGTCCAATATCTCAAGGAAACATCAGAGTTGCATGCTATGAATTGTGTTCCTGCATTTACTCAGGCGCATGATGTACAAGAAGCTCTTAATGAAAAACTTGAAAGTTCCGGACCGGGTCCTCAAACAAATAATAATGTTTCAAATAATGGTTATGCTGAAATTGGCTCAATTAGGTGTTCAGTTCCAAAGGATGTAGCATTGCCAAGAATCATATTCTGCCTAGCTCACCATGGAAAGGTTGCTTGGGATGTGAAATGGCGTCCTCTTAATGAACATGATTCCAGATGCAAGCATCGAATGGGCTATCTTGCTGTTCTGTTGGGGAACGGGTCTCTGGAAGTGTGGGAGGTTCCTGTTCCTCGTGCAATTGAAGTTATTTATTCTTCTTCATCTGGAGAGGGCACTGATCCTCGCTTTGTAAAATTGGCACCAGTATTTAGATGCTCAATGTTGAAGTCTGGTGACAAAAAAAGCATCCCTCTTACTGTGGAATGGTCAGCTTCACCACCTCACGATTACTTAATTGCCGGATGCCATGATGGAACTGTTGCTATGTGGAAGTTTTCCGCTAGTAATGTATCTCAAGATACAAGGCCTTTACTTTGTTTCAGCGCAGATACAAATCCTATTAGGGCGCTCTCTTGGGCCCCAGCTCAAAGCAATTCAGACAATGCAAATGTTATAGCTACTGCTGGACATGGAGGCCTAAAATTTTGGGACCTACGTGATCCATTCCGTCCCTTGTGGGACATCGATCATATACCAAGGTTCATATATAGTCTGGATTGGATTCCAGATCCAAGGTGTGTTCTTCTCTCCTTTGATGATGGAACAATGAGACTTCTCAGCTTGACAAAGGCTGCCTCTGATGCCCCTGTAACAGGAAAACCCTTTGCTGGAACAAAACAACAGGGCCTGCACAATCTTGGTTGTTTGCCTTTTGCTATCTGGAGTGTTCAAGTATCAAGACTAACAGGTATGGTTGCATATTGTGGTGCAGATGGAACTGTTCTGCGCTTCCAACTTACGTCCAATGCTGTGGAGAAAGATGCTACGCGAAACCGGGCCCCACATTTTCTGTGCGTGTCACTAACTGAAGAGGACTCTGTTGTGACCATCAATACCCCAGTACCATATAATCCTTTCCCGTTGAAAACATCTCGTAAAGCTGAGCCAAACAAAGTGAAGAGAGAACATGATAAGATGACAACTACATCACAAGATCAAGTTTTAGCTCTTTGTTACGGTGATGATCCGGGTATTGAATTAGAATCTGGGAAAGAAGCAGCATCTCTTAGAAGCAAAACACTCAAATCTGCGGATGACCAAGCATTAGTATGCATGGATCAAGAGCCATTTAATACCCAAGACGAAGAAATTGGAGAAAAGGGTACATCTCTTAAAGGAGTTGTAAAACAGAAATCCAAGTCTAGTAAGAAAAATACAGAGGATGAACAAGAACCGGAAGGCAGAGATGAAGAGCTGATTAATACGCAGAGAGAAAAAACTGGGACCGAGTATGAAGTTTTTCCTTCCAAGCAAGTAGCAATGCACCGAGTGAGATGGAACACGAACAAGGGTAGTGAGAGATGGTTATGCTATGGAGGGGCAGCTGGAATAGTACGTTGCCAAGAGATTGTTTTGTCTGACATTGATATGAAATGGGCAAGGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

1110

Amino Acids

123.77

Weight (kDa)

6.0

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 713 - 839 1e-05 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 662, 2080
AccB7I CCANNNNNTGG 1 cut(s) 653
AccII CGCG 3 cut(s) 54, 470, 2710
AccIII TCCGGA 1 cut(s) 1760
AciI CCGC 9 cut(s) 54, 69, 95, 146, 433, 470, 1640, 2220, 2972
AclWI GGATC 6 cut(s) 628, 2054, 2375, 2444, 2900, 3006
AcsI RAATTY 2 cut(s) 1099, 2363
AcuI CTGAAG 1 cut(s) 2769
AcyI GRCGYC 2 cut(s) 270, 1912
AdeI CACNNNGTG 1 cut(s) 3211
AfaI GTAC 8 cut(s) 1107, 1320, 1515, 1547, 1728, 2784, 3046, 3276
AfiI CCNNNNNNNGG 7 cut(s) 29, 653, 737, 1639, 1888, 2534, 2549
AjnI CCWGG 2 cut(s) 616, 1582
AjuI GAANNNNNNNTTGG 4 cut(s) 804, 836, 1879, 1911
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 4 cut(s) 490, 717, 1989, 2485
AlwI GGATC 6 cut(s) 628, 2054, 2375, 2444, 2900, 3006
AlwNI CAGNNNCTG 2 cut(s) 131, 2569
Ama87I CYCGRG 2 cut(s) 29, 1557
Aor13HI TCCGGA 1 cut(s) 1760
ApaI GGGCCC 2 cut(s) 2302, 2722
ApeKI GCWGC 6 cut(s) 605, 1416, 1629, 2513, 2939, 3263
ApoI RAATTY 2 cut(s) 1099, 2363
AseI ATTAAT 2 cut(s) 974, 3141
Asp700I GAANNNNTTC 2 cut(s) 711, 3180
AspLEI GCGC 7 cut(s) 54, 188, 1487, 1720, 2267, 2290, 2670
AsuC2I CCSGG 4 cut(s) 290, 1767, 2717, 2910
AsuHPI GGTGA 7 cut(s) 304, 653, 1439, 1871, 2126, 2148, 2912
AvaI CYCGRG 2 cut(s) 29, 1557
AvaII GGWCC 4 cut(s) 1763, 1769, 2371, 3165
BaeGI GKGCMC 3 cut(s) 2057, 2302, 2722
BaeI ACNNNNGTAYC 2 cut(s) 2766, 2799
BanI GGYRCC 2 cut(s) 662, 2080
BanII GRGCYC 3 cut(s) 261, 2302, 2722
BauI CACGAG 1 cut(s) 2013
BbsI GAAGAC 8 cut(s) 282, 674, 796, 880, 964, 1070, 1177, 1283
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 6 cut(s) 592, 1428, 1641, 2500, 2951, 3275
BccI CCATC 8 cut(s) 995, 1208, 2186, 2474, 2648, 2777, 3210, 3237
BcgI CGANNNNNNTGC 6 cut(s) 363, 397, 560, 578, 594, 612
BciT130I CCWGG 2 cut(s) 618, 1584
BclI TGATCA 1 cut(s) 1441
BcnI CCSGG 4 cut(s) 290, 1767, 2717, 2910
BcoDI GTCTC 4 cut(s) 490, 717, 1989, 2485
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 2 cut(s) 189, 2291
BglI GCCNNNNNGGC 1 cut(s) 862
BisI GCNGC 7 cut(s) 434, 606, 1417, 1630, 2514, 2940, 3264
BlpI GCTNAGC 1 cut(s) 2823
BlsI GCNGC 7 cut(s) 435, 607, 1418, 1631, 2515, 2941, 3265
Bme1390I CCNGG 6 cut(s) 290, 618, 1584, 1767, 2717, 2910
Bme18I GGWCC 4 cut(s) 1763, 1769, 2371, 3165
BmeT110I CYCGRG 2 cut(s) 29, 1557
BmrFI CCNGG 6 cut(s) 290, 618, 1584, 1767, 2717, 2910
BmrI ACTGGG 2 cut(s) 2774, 3171
BmsI GCATC 9 cut(s) 133, 1928, 1954, 2084, 2136, 2174, 2512, 2692, 2951
BmuI ACTGGG 2 cut(s) 2774, 3171
BpiI GAAGAC 8 cut(s) 282, 674, 796, 880, 964, 1070, 1177, 1283
BpmI CTGGAG 4 cut(s) 471, 1289, 2067, 2623
Bpu1102I GCTNAGC 1 cut(s) 2823
BpuEI CTTGAG 7 cut(s) 160, 352, 1077, 1290, 1485, 1649, 2220
BpuMI CCSGG 4 cut(s) 290, 1767, 2717, 2910
Bsa29I ATCGAT 2 cut(s) 141, 2406
BsaAI YACGTR 1 cut(s) 2378
BsaBI GATNNNNATC 2 cut(s) 1446, 2274
BsaHI GRCGYC 2 cut(s) 270, 1912
BsaI GGTCTC 1 cut(s) 1989
BsaJI CCNNGG 5 cut(s) 616, 1583, 1882, 2417, 2453
BsaWI WCCGGW 2 cut(s) 1760, 3115
BsaXI ACNNNNNCTCC 6 cut(s) 469, 499, 513, 543, 1497, 1527
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 653, 737, 1639, 1888, 2534, 2549
Bse1I ACTGG 5 cut(s) 658, 1272, 2084, 2780, 3166
Bse3DI GCAATG 3 cut(s) 600, 1850, 3207
Bse8I GATNNNNATC 2 cut(s) 1446, 2274
BseAI TCCGGA 1 cut(s) 1760
BseBI CCWGG 2 cut(s) 618, 1584
BseCI ATCGAT 2 cut(s) 141, 2406
BseDI CCNNGG 5 cut(s) 616, 1583, 1882, 2417, 2453
BseJI GATNNNNATC 2 cut(s) 1446, 2274
BseLI CCNNNNNNNGG 7 cut(s) 29, 653, 737, 1639, 1888, 2534, 2549
BseMI GCAATG 3 cut(s) 600, 1850, 3207
BseMII CTCAG 6 cut(s) 246, 843, 1047, 1727, 2512, 2814
BseNI ACTGG 5 cut(s) 658, 1272, 2084, 2780, 3166
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 3 cut(s) 2057, 2302, 2722
BseXI GCAGC 6 cut(s) 592, 1428, 1641, 2500, 2951, 3275
BseYI CCCAGC 1 cut(s) 2302
BsgI GTGCAG 2 cut(s) 2555, 2670
Bsh1236I CGCG 3 cut(s) 54, 470, 2710
Bsh1285I CGRYCG 2 cut(s) 141, 574
BshNI GGYRCC 2 cut(s) 662, 2080
BshVI ATCGAT 2 cut(s) 141, 2406
BsiEI CGRYCG 2 cut(s) 141, 574
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 2 cut(s) 29, 1557
BsiSI CCGG 7 cut(s) 290, 1761, 1766, 2181, 2716, 2909, 3116
BslFI GGGAC 5 cut(s) 119, 2376, 2384, 2414, 3178
BslI CCNNNNNNNGG 7 cut(s) 29, 653, 737, 1639, 1888, 2534, 2549
BsmAI GTCTC 4 cut(s) 490, 717, 1989, 2485
BsmFI GGGAC 5 cut(s) 119, 2376, 2384, 2414, 3178
BsmI GAATGC 2 cut(s) 1063, 1276
Bso31I GGTCTC 1 cut(s) 1989
BsoBI CYCGRG 2 cut(s) 29, 1557
Bsp120I GGGCCC 2 cut(s) 2298, 2718
Bsp1286I GDGCHC 4 cut(s) 261, 2057, 2302, 2722
Bsp13I TCCGGA 1 cut(s) 1760
Bsp1407I TGTACA 3 cut(s) 1105, 1318, 1726
Bsp1720I GCTNAGC 1 cut(s) 2823
Bsp19I CCATGG 1 cut(s) 1882
BspACI CCGC 9 cut(s) 54, 69, 95, 146, 433, 470, 1640, 2220, 2972
BspCNI CTCAG 6 cut(s) 247, 844, 1048, 1726, 2511, 2815
BspDI ATCGAT 2 cut(s) 141, 2406
BspEI TCCGGA 1 cut(s) 1760
BspFNI CGCG 3 cut(s) 54, 470, 2710
BspHI TCATGA 1 cut(s) 559
BspPI GGATC 6 cut(s) 628, 2054, 2375, 2444, 2900, 3006
BspQI GCTCTTC 1 cut(s) 3126
BspT107I GGYRCC 2 cut(s) 662, 2080
BspTNI GGTCTC 1 cut(s) 1989
BsrDI GCAATG 3 cut(s) 600, 1850, 3207
BsrGI TGTACA 3 cut(s) 1105, 1318, 1726
BsrI ACTGG 5 cut(s) 658, 1272, 2084, 2780, 3166
BssECI CCNNGG 5 cut(s) 616, 1583, 1882, 2417, 2453
BssNI GRCGYC 2 cut(s) 270, 1912
BssSI CACGAG 1 cut(s) 2013
BssT1I CCWWGG 3 cut(s) 1882, 2417, 2453
Bst2BI CACGAG 1 cut(s) 2013
Bst2UI CCWGG 2 cut(s) 618, 1584
Bst4CI ACNGT 7 cut(s) 454, 575, 849, 2140, 2200, 2662, 2900
Bst6I CTCTTC 5 cut(s) 3, 918, 2745, 2834, 3126
BstACI GRCGYC 2 cut(s) 270, 1912
BstAPI GCANNNNNTGC 1 cut(s) 1482
BstAUI TGTACA 3 cut(s) 1105, 1318, 1726
BstBAI YACGTR 1 cut(s) 2378
BstC8I GCNNGC 5 cut(s) 82, 382, 1686, 1943, 2570
BstDEI CTNAG 8 cut(s) 255, 852, 1056, 1133, 1713, 2498, 2823, 2948
BstDSI CCRYGG 1 cut(s) 1882
BstENI CCTNNNNNAGG 1 cut(s) 2532
BstFNI CGCG 3 cut(s) 54, 470, 2710
BstH2I RGCGCY 2 cut(s) 189, 2291
BstHHI GCGC 7 cut(s) 54, 188, 1487, 1720, 2267, 2290, 2670
BstMAI GTCTC 4 cut(s) 490, 717, 1989, 2485
BstMCI CGRYCG 2 cut(s) 141, 574
BstMWI GCNNNNNNNGC 6 cut(s) 77, 386, 605, 862, 1482, 2522
BstNI CCWGG 2 cut(s) 618, 1584
BstNSI RCATGY 1 cut(s) 1688
BstSCI CCNGG 6 cut(s) 288, 616, 1582, 1765, 2715, 2908
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 3 cut(s) 2057, 2302, 2722
BstUI CGCG 3 cut(s) 54, 470, 2710
BstV1I GCAGC 6 cut(s) 592, 1428, 1641, 2500, 2951, 3275
BstV2I GAAGAC 8 cut(s) 282, 674, 796, 880, 964, 1070, 1177, 1283
BstX2I RGATCY 2 cut(s) 620, 2449
BstXI CCANNNNNNTGG 1 cut(s) 2692
BstYI RGATCY 2 cut(s) 620, 2449
Bsu15I ATCGAT 2 cut(s) 141, 2406
BsuTUI ATCGAT 2 cut(s) 141, 2406
BtgI CCRYGG 1 cut(s) 1882
BtsIMutI CAGTG 3 cut(s) 771, 1265, 2055
Cac8I GCNNGC 5 cut(s) 82, 382, 1686, 1943, 2570
CaiI CAGNNNCTG 2 cut(s) 131, 2569
CciI TCATGA 1 cut(s) 559
CfoI GCGC 7 cut(s) 54, 188, 1487, 1720, 2267, 2290, 2670
ClaI ATCGAT 2 cut(s) 141, 2406
CpoI CGGWCCG 1 cut(s) 1763
CseI GACGC 4 cut(s) 278, 476, 1322, 1901
Csp6I GTAC 8 cut(s) 1106, 1319, 1514, 1546, 1727, 2783, 3045, 3275
CspI CGGWCCG 1 cut(s) 1763
CviQI GTAC 8 cut(s) 1106, 1319, 1514, 1546, 1727, 2783, 3045, 3275
DdeI CTNAG 8 cut(s) 255, 852, 1056, 1133, 1713, 2498, 2823, 2948
DraIII CACNNNGTG 1 cut(s) 3211
Eam1104I CTCTTC 5 cut(s) 3, 918, 2745, 2834, 3126
EarI CTCTTC 5 cut(s) 3, 918, 2745, 2834, 3126
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 3 cut(s) 1882, 2417, 2453
Eco147I AGGCCT 4 cut(s) 949, 1357, 2248, 2358
Eco24I GRGCYC 3 cut(s) 261, 2302, 2722
Eco31I GGTCTC 1 cut(s) 1989
Eco47I GGWCC 4 cut(s) 1763, 1769, 2371, 3165
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 2769
Eco88I CYCGRG 2 cut(s) 29, 1557
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 2532
EcoO109I RGGNCCY 6 cut(s) 524, 1769, 2299, 2371, 2566, 2719
EcoRII CCWGG 2 cut(s) 616, 1582
EcoT14I CCWWGG 3 cut(s) 1882, 2417, 2453
EcoT22I ATGCAT 1 cut(s) 2996
EcoT38I GRGCYC 3 cut(s) 261, 2302, 2722
ErhI CCWWGG 3 cut(s) 1882, 2417, 2453
FalI AAGNNNNNCTT 2 cut(s) 1722, 1754
FaqI GGGAC 5 cut(s) 119, 2376, 2384, 2414, 3178
FauI CCCGC 2 cut(s) 47, 463
FbaI TGATCA 1 cut(s) 1441
Fnu4HI GCNGC 7 cut(s) 434, 606, 1417, 1630, 2514, 2940, 3264
FriOI GRGCYC 3 cut(s) 261, 2302, 2722
Fsp4HI GCNGC 7 cut(s) 434, 606, 1417, 1630, 2514, 2940, 3264
GlaI GCGC 7 cut(s) 53, 187, 1486, 1719, 2266, 2289, 2669
GluI GCNGC 7 cut(s) 434, 606, 1417, 1630, 2514, 2940, 3264
GsaI CCCAGC 1 cut(s) 2306
GsuI CTGGAG 4 cut(s) 471, 1289, 2067, 2623
HaeII RGCGCY 2 cut(s) 189, 2291
HapII CCGG 7 cut(s) 290, 1761, 1766, 2181, 2716, 2909, 3116
HgaI GACGC 4 cut(s) 278, 476, 1322, 1901
HhaI GCGC 7 cut(s) 54, 188, 1487, 1720, 2267, 2290, 2670
Hin1I GRCGYC 2 cut(s) 270, 1912
Hin6I GCGC 7 cut(s) 52, 186, 1485, 1718, 2265, 2288, 2668
HinP1I GCGC 7 cut(s) 52, 186, 1485, 1718, 2265, 2288, 2668
HincII GTYRAC 3 cut(s) 991, 1151, 1204
HindII GTYRAC 3 cut(s) 991, 1151, 1204
HpaII CCGG 7 cut(s) 290, 1761, 1766, 2181, 2716, 2909, 3116
HphI GGTGA 7 cut(s) 304, 653, 1439, 1871, 2126, 2148, 2912
Hpy166II GTNNAC 4 cut(s) 991, 1151, 1204, 1312
Hpy188I TCNGA 9 cut(s) 256, 400, 672, 853, 1057, 1678, 2320, 2521, 3301
Hpy8I GTNNAC 4 cut(s) 991, 1151, 1204, 1312
Hpy99I CGWCG 2 cut(s) 272, 470
HpyAV CCTTC 3 cut(s) 701, 3113, 3195
HpyCH4III ACNGT 7 cut(s) 454, 575, 849, 2140, 2200, 2662, 2900
HpyCH4IV ACGT 4 cut(s) 1544, 2377, 2681, 3277
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 386, 605, 862, 1482, 2522
HpyF3I CTNAG 8 cut(s) 255, 852, 1056, 1133, 1713, 2498, 2823, 2948
HpySE526I ACGT 4 cut(s) 1544, 2377, 2681, 3277
Hsp92I GRCGYC 2 cut(s) 270, 1912
HspAI GCGC 7 cut(s) 52, 186, 1485, 1718, 2265, 2288, 2668
Kpn2I TCCGGA 1 cut(s) 1760
Ksp22I TGATCA 1 cut(s) 1441
LguI GCTCTTC 1 cut(s) 3126
LmnI GCTCC 4 cut(s) 148, 755, 1492, 1626
Lsp1109I GCAGC 6 cut(s) 592, 1428, 1641, 2500, 2951, 3275
LweI GCATC 9 cut(s) 133, 1928, 1954, 2084, 2136, 2174, 2512, 2692, 2951
MaeII ACGT 4 cut(s) 1544, 2377, 2681, 3277
MaeIII GTNAC 8 cut(s) 88, 566, 1331, 2114, 2530, 2739, 2764, 2894
MfeI CAATTG 4 cut(s) 153, 159, 1012, 2019
MflI RGATCY 2 cut(s) 620, 2449
MhlI GDGCHC 4 cut(s) 261, 2057, 2302, 2722
MlyI GAGTC 7 cut(s) 209, 246, 1156, 1336, 1549, 1662, 2750
MmeI TCCRAC 3 cut(s) 499, 1449, 2698
Mph1103I ATGCAT 1 cut(s) 2996
MroI TCCGGA 1 cut(s) 1760
MroXI GAANNNNTTC 2 cut(s) 711, 3180
MseI TTAA 8 cut(s) 351, 974, 1740, 1920, 2174, 3012, 3054, 3141
MslI CAYNNNNRTG 2 cut(s) 2989, 3308
MspA1I CMGCKG 1 cut(s) 3266
MspI CCGG 7 cut(s) 290, 1761, 1766, 2181, 2716, 2909, 3116
MspR9I CCNGG 6 cut(s) 290, 618, 1584, 1767, 2717, 2910
MunI CAATTG 4 cut(s) 153, 159, 1012, 2019
Mva1269I GAATGC 2 cut(s) 1063, 1276
MvaI CCWGG 2 cut(s) 618, 1584
MvnI CGCG 3 cut(s) 54, 470, 2710
MwoI GCNNNNNNNGC 6 cut(s) 77, 386, 605, 862, 1482, 2522
NciI CCSGG 4 cut(s) 290, 1767, 2717, 2910
NcoI CCATGG 1 cut(s) 1882
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 4 cut(s) 566, 2114, 2739, 2764
NsiI ATGCAT 1 cut(s) 2996
NspI RCATGY 1 cut(s) 1688
PaeI GCATGC 1 cut(s) 1688
PaeR7I CTCGAG 1 cut(s) 1557
PagI TCATGA 1 cut(s) 559
PceI AGGCCT 4 cut(s) 949, 1357, 2248, 2358
PciSI GCTCTTC 1 cut(s) 3126
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 2 cut(s) 1063, 1276
PdmI GAANNNNTTC 2 cut(s) 711, 3180
PflFI GACNNNGTC 1 cut(s) 1767
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 7 cut(s) 435, 607, 1418, 1631, 2515, 2941, 3265
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 7 cut(s) 209, 245, 1155, 1336, 1549, 1661, 2750
PpsI GAGTC 7 cut(s) 209, 245, 1155, 1336, 1549, 1661, 2750
Ppu21I YACGTR 1 cut(s) 2378
PpuMI RGGWCCY 2 cut(s) 1769, 2371
PshBI ATTAAT 2 cut(s) 974, 3141
Psp124BI GAGCTC 1 cut(s) 261
Psp5II RGGWCCY 2 cut(s) 1769, 2371
Psp6I CCWGG 2 cut(s) 616, 1582
PspFI CCCAGC 1 cut(s) 2302
PspGI CCWGG 2 cut(s) 616, 1582
PspOMI GGGCCC 2 cut(s) 2298, 2718
PspPPI RGGWCCY 2 cut(s) 1769, 2371
PspXI VCTCGAGB 1 cut(s) 1557
PstNI CAGNNNCTG 2 cut(s) 131, 2569
PsuI RGATCY 2 cut(s) 620, 2449
PsyI GACNNNGTC 1 cut(s) 1767
PvuI CGATCG 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 3266
RsaI GTAC 8 cut(s) 1107, 1320, 1515, 1547, 1728, 2784, 3046, 3276
RsaNI GTAC 8 cut(s) 1106, 1319, 1514, 1546, 1727, 2783, 3045, 3275
RseI CAYNNNNRTG 2 cut(s) 2989, 3308
Rsr2I CGGWCCG 1 cut(s) 1763
RsrII CGGWCCG 1 cut(s) 1763
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 3126
SaqAI TTAA 8 cut(s) 351, 974, 1740, 1920, 2174, 3012, 3054, 3141
SatI GCNGC 7 cut(s) 434, 606, 1417, 1630, 2514, 2940, 3264
SchI GAGTC 7 cut(s) 209, 246, 1156, 1336, 1549, 1662, 2750
ScrFI CCNGG 6 cut(s) 290, 618, 1584, 1767, 2717, 2910
SduI GDGCHC 4 cut(s) 261, 2057, 2302, 2722
SfaNI GCATC 9 cut(s) 133, 1928, 1954, 2084, 2136, 2174, 2512, 2692, 2951
SfcI CTRYAG 1 cut(s) 717
Sfr274I CTCGAG 1 cut(s) 1557
SinI GGWCC 4 cut(s) 1763, 1769, 2371, 3165
SlaI CTCGAG 1 cut(s) 1557
SmiMI CAYNNNNRTG 2 cut(s) 2989, 3308
SmlI CTYRAG 8 cut(s) 175, 331, 1092, 1305, 1500, 1557, 1664, 2235
SmoI CTYRAG 8 cut(s) 175, 331, 1092, 1305, 1500, 1557, 1664, 2235
SphI GCATGC 1 cut(s) 1688
SseBI AGGCCT 4 cut(s) 949, 1357, 2248, 2358
SsiI CCGC 9 cut(s) 54, 69, 95, 146, 433, 470, 1640, 2220, 2972
SspI AATATT 1 cut(s) 1604
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 4 cut(s) 949, 1357, 2248, 2358
StyD4I CCNGG 6 cut(s) 288, 616, 1582, 1765, 2715, 2908
StyI CCWWGG 3 cut(s) 1882, 2417, 2453
TaaI ACNGT 7 cut(s) 454, 575, 849, 2140, 2200, 2662, 2900
TaiI ACGT 4 cut(s) 1547, 2380, 2684, 3280
TaqII GACCGA 1 cut(s) 3182
TatI WGTACW 3 cut(s) 1105, 1318, 1726
TauI GCSGC 1 cut(s) 436
Tru1I TTAA 8 cut(s) 351, 974, 1740, 1920, 2174, 3012, 3054, 3141
Tru9I TTAA 8 cut(s) 351, 974, 1740, 1920, 2174, 3012, 3054, 3141
TscAI CASTG 3 cut(s) 778, 1272, 2062
TseFI GTSAC 4 cut(s) 566, 2114, 2739, 2764
TseI GCWGC 6 cut(s) 605, 1416, 1629, 2513, 2939, 3263
Tsp45I GTSAC 4 cut(s) 566, 2114, 2739, 2764
TspGWI ACGGA 2 cut(s) 1298, 2378
TspRI CASTG 3 cut(s) 778, 1272, 2062
Tth111I GACNNNGTC 1 cut(s) 1767
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 4 cut(s) 1763, 1769, 2371, 3165
VspI ATTAAT 2 cut(s) 974, 3141
XagI CCTNNNNNAGG 1 cut(s) 2532
XapI RAATTY 2 cut(s) 1099, 2363
XceI RCATGY 1 cut(s) 1688
XhoI CTCGAG 1 cut(s) 1557
XmnI GAANNNNTTC 2 cut(s) 711, 3180
Zsp2I ATGCAT 1 cut(s) 2996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.