Rorug04G0092500

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002231
Physical Location & Seq
Forward (+)
14302282 .. 14303028
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0092500.1

Sequence Viewer

Length: 747 bp
ATGGCTAAATGGATCCTTCTTCTCGTCACTTTTGTTCAACCAACGGTGCTGTGTGGGGGTTATTTGGATTTCGGAGATGGTCTACTCTTTATAGAGCAACGTTTGTTTGTTGACAAGGGTTGGTGGCTGGCAGAGACTCTGGCGCCGCTCTCTGATGGCTTTCAACCGGGGCTTCTTGGCCTGGATTTGGTGGCTACAGGTGGTAATGGTGATGGCAACAAAAATGTCAAAATGGTGAAGAACGATGGAGTCAGGTTCAGAGGTGGACGGATTGGTGTAGGGGTGACTGCCGACAACTGTACTGCCCTGTTTCAATCTATGTTCATTGGAGAGGCTAACGGCTGGGAAGAGCTATTTCGGTCAGATCTGTGGAGGTGCTATGCTGCGGTGACGTATGTCAGAAGGGATGATGATCTGGATTTTGGTGGTTGCAGACCGGCAGACAAGGATGGTCGCGGTGATTCTACAGGCGTTGGGCTAGGTGGTCTGCAATGTCGATGTCATCTGGCAGCGGCAAAGGTTGCCAGTGGTGATGGTCTGGTATTCCATGGAGAACGCTATGGGGTGCCCTTAGCATTTCCTGATTGGGTGCCTTTAGGGTTTCTATACCTTGGGCTATTTGGGCCAGGCTTGCTTTGGATGGTGATGTGGACGTCATGGGCTCGAGCAGAATTGGAAGGGGAAACTGCTACTCCTCTTCTGAAAATTATTTTATTTTTATTAGGTCGCAAAGATCATAATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

248

Amino Acids

27.08

Weight (kDa)

5.23

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 656
AccB1I GGYRCC 3 cut(s) 142, 565, 589
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 82
AccII CGCG 1 cut(s) 456
AciI CCGC 4 cut(s) 146, 386, 456, 512
AclI AACGTT 1 cut(s) 100
AclWI GGATC 2 cut(s) 7, 20
AcyI GRCGYC 2 cut(s) 143, 653
AfaI GTAC 1 cut(s) 301
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 3 cut(s) 38, 164, 314
AjnI CCWGG 2 cut(s) 180, 625
AluBI AGCT 1 cut(s) 352
AluI AGCT 1 cut(s) 352
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 7, 20
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 2 cut(s) 178, 623
ApeKI GCWGC 2 cut(s) 383, 509
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 1 cut(s) 623
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 7 cut(s) 221, 247, 295, 400, 470, 542, 655
AvaI CYCGRG 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 570
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 3 cut(s) 142, 565, 589
BanII GRGCYC 1 cut(s) 664
BbvI GCAGC 2 cut(s) 370, 521
BccI CCATC 7 cut(s) 71, 149, 206, 239, 443, 527, 634
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 2 cut(s) 182, 627
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 195, 465
BfoI RGCGCY 1 cut(s) 146
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 146, 384, 510, 513
BlsI GCNGC 4 cut(s) 147, 385, 511, 514
Bme1390I CCNGG 3 cut(s) 168, 182, 627
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 623
BmiI GGNNCC 4 cut(s) 14, 144, 567, 591
BmrFI CCNGG 3 cut(s) 168, 182, 627
BoxI GACNNNNGTC 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 168
BsaBI GATNNNNATC 2 cut(s) 411, 738
BsaHI GRCGYC 2 cut(s) 143, 653
BsaJI CCNNGG 3 cut(s) 167, 547, 610
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 1 cut(s) 525
Bse3DI GCAATG 1 cut(s) 497
Bse8I GATNNNNATC 2 cut(s) 411, 738
BseBI CCWGG 2 cut(s) 182, 627
BseDI CCNNGG 3 cut(s) 167, 547, 610
BseGI GGATG 3 cut(s) 412, 454, 645
BseJI GATNNNNATC 2 cut(s) 411, 738
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMI GCAATG 1 cut(s) 497
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 2 cut(s) 370, 521
BseYI CCCAGC 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 180, 625
BshNI GGYRCC 3 cut(s) 142, 565, 589
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 2 cut(s) 167, 437
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 180, 625
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 2 cut(s) 570, 664
Bsp143I GATC 4 cut(s) 12, 364, 412, 733
Bsp19I CCATGG 1 cut(s) 547
BspACI CCGC 4 cut(s) 146, 386, 456, 512
BspANI GGCC 2 cut(s) 180, 625
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 4 cut(s) 14, 144, 567, 591
BspPI GGATC 2 cut(s) 7, 20
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 3 cut(s) 142, 565, 589
BsrBI CCGCTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 3 cut(s) 167, 547, 610
BssMI GATC 4 cut(s) 12, 364, 412, 733
BssNI GRCGYC 2 cut(s) 143, 653
BssT1I CCWWGG 2 cut(s) 547, 610
Bst2UI CCWGG 2 cut(s) 182, 627
Bst4CI ACNGT 2 cut(s) 46, 299
Bst6I CTCTTC 2 cut(s) 342, 702
BstACI GRCGYC 2 cut(s) 143, 653
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 129, 632
BstDEI CTNAG 1 cut(s) 571
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 3 cut(s) 412, 454, 645
BstFNI CGCG 1 cut(s) 456
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 4 cut(s) 15, 367, 415, 736
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 4 cut(s) 12, 364, 412, 733
BstMWI GCNNNNNNNGC 3 cut(s) 521, 622, 631
BstNI CCWGG 2 cut(s) 182, 627
BstPAI GACNNNNGTC 1 cut(s) 395
BstSCI CCNGG 3 cut(s) 166, 180, 625
BstSFI CTRYAG 2 cut(s) 195, 465
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 370, 521
BstX2I RGATCY 2 cut(s) 12, 364
BstYI RGATCY 2 cut(s) 12, 364
BsuRI GGCC 2 cut(s) 180, 625
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 3 cut(s) 412, 454, 645
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 129, 632
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 300
CviAII CATG 2 cut(s) 548, 657
CviQI GTAC 1 cut(s) 300
DdeI CTNAG 1 cut(s) 571
DinI GGCGCC 1 cut(s) 144
DpnI GATC 4 cut(s) 14, 366, 414, 735
DpnII GATC 4 cut(s) 12, 364, 412, 733
Eam1104I CTCTTC 2 cut(s) 342, 702
EarI CTCTTC 2 cut(s) 342, 702
Eco130I CCWWGG 2 cut(s) 547, 610
Eco24I GRGCYC 1 cut(s) 664
Eco88I CYCGRG 1 cut(s) 663
EcoRII CCWGG 2 cut(s) 180, 625
EcoT14I CCWWGG 2 cut(s) 547, 610
EcoT38I GRGCYC 1 cut(s) 664
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
ErhI CCWWGG 2 cut(s) 547, 610
FaeI CATG 2 cut(s) 551, 660
FaiI YATR 9 cut(s) 92, 320, 381, 396, 549, 561, 607, 658, 738
FatI CATG 2 cut(s) 547, 656
FblI GTMKAC 1 cut(s) 82
Fnu4HI GCNGC 4 cut(s) 146, 384, 510, 513
FokI GGATG 3 cut(s) 419, 461, 652
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 4 cut(s) 146, 384, 510, 513
FspBI CTAG 1 cut(s) 479
GlaI GCGC 1 cut(s) 144
GluI GCNGC 4 cut(s) 146, 384, 510, 513
GsaI CCCAGC 1 cut(s) 346
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 2 cut(s) 180, 625
HapII CCGG 2 cut(s) 167, 437
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 2 cut(s) 143, 653
Hin1II CATG 2 cut(s) 551, 660
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 3 cut(s) 136, 249, 461
HpaII CCGG 2 cut(s) 167, 437
HphI GGTGA 7 cut(s) 221, 247, 295, 400, 470, 542, 655
Hpy166II GTNNAC 4 cut(s) 83, 112, 266, 651
Hpy188I TCNGA 7 cut(s) 74, 154, 260, 364, 401, 702, 746
Hpy188III TCNNGA 2 cut(s) 416, 581
Hpy8I GTNNAC 4 cut(s) 83, 112, 266, 651
HpyAV CCTTC 3 cut(s) 26, 396, 671
HpyCH4III ACNGT 2 cut(s) 46, 299
HpyCH4IV ACGT 3 cut(s) 100, 392, 653
HpyCH4V TGCA 2 cut(s) 432, 490
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 622, 631
HpyF3I CTNAG 1 cut(s) 571
HpySE526I ACGT 3 cut(s) 100, 392, 653
Hsp92I GRCGYC 2 cut(s) 143, 653
Hsp92II CATG 2 cut(s) 551, 660
HspAI GCGC 1 cut(s) 143
KasI GGCGCC 1 cut(s) 142
Kzo9I GATC 4 cut(s) 12, 364, 412, 733
LguI GCTCTTC 1 cut(s) 342
Lsp1109I GCAGC 2 cut(s) 370, 521
MaeI CTAG 1 cut(s) 479
MaeII ACGT 3 cut(s) 100, 392, 653
MaeIII GTNAC 3 cut(s) 25, 283, 388
MalI GATC 4 cut(s) 14, 366, 414, 735
MbiI CCGCTC 1 cut(s) 148
MboI GATC 4 cut(s) 12, 364, 412, 733
MboII GAAGA 4 cut(s) 11, 250, 359, 689
MflI RGATCY 2 cut(s) 12, 364
MhlI GDGCHC 2 cut(s) 570, 664
MluCI AATT 2 cut(s) 671, 705
Mly113I GGCGCC 1 cut(s) 143
MlyI GAGTC 2 cut(s) 130, 258
MnlI CCTC 4 cut(s) 254, 325, 366, 705
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 2 cut(s) 167, 437
MspR9I CCNGG 3 cut(s) 168, 182, 627
MvaI CCWGG 2 cut(s) 182, 627
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 521, 622, 631
NarI GGCGCC 1 cut(s) 143
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 547
NdeII GATC 4 cut(s) 12, 364, 412, 733
NlaIII CATG 2 cut(s) 551, 660
NlaIV GGNNCC 4 cut(s) 14, 144, 567, 591
NmuCI GTSAC 3 cut(s) 25, 283, 388
PaeR7I CTCGAG 1 cut(s) 663
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 461
PkrI GCNGC 4 cut(s) 147, 385, 511, 514
PleI GAGTC 2 cut(s) 130, 257
PluTI GGCGCC 1 cut(s) 146
PpsI GAGTC 2 cut(s) 130, 257
PshAI GACNNNNGTC 1 cut(s) 395
Psp1406I AACGTT 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 180, 625
PspFI CCCAGC 1 cut(s) 342
PspGI CCWGG 2 cut(s) 180, 625
PspN4I GGNNCC 4 cut(s) 14, 144, 567, 591
PspPI GGNCC 1 cut(s) 623
PspXI VCTCGAGB 1 cut(s) 663
PsuI RGATCY 2 cut(s) 12, 364
RsaI GTAC 1 cut(s) 301
RsaNI GTAC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 342
SatI GCNGC 4 cut(s) 146, 384, 510, 513
Sau3AI GATC 4 cut(s) 12, 364, 412, 733
Sau96I GGNCC 1 cut(s) 623
SchI GAGTC 2 cut(s) 130, 258
ScrFI CCNGG 3 cut(s) 168, 182, 627
SduI GDGCHC 2 cut(s) 570, 664
SfcI CTRYAG 2 cut(s) 195, 465
SfoI GGCGCC 1 cut(s) 144
Sfr274I CTCGAG 1 cut(s) 663
SlaI CTCGAG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 2 cut(s) 671, 705
SsiI CCGC 4 cut(s) 146, 386, 456, 512
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 3 cut(s) 166, 180, 625
StyI CCWWGG 2 cut(s) 547, 610
TaaI ACNGT 2 cut(s) 46, 299
TaiI ACGT 3 cut(s) 103, 395, 656
TaqI TCGA 2 cut(s) 496, 664
TaqII GACCGA 1 cut(s) 348
TasI AATT 2 cut(s) 671, 705
TatI WGTACW 1 cut(s) 299
TauI GCSGC 2 cut(s) 148, 515
TfiI GAWTC 1 cut(s) 461
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 3 cut(s) 25, 283, 388
TseI GCWGC 2 cut(s) 383, 509
Tsp45I GTSAC 3 cut(s) 25, 283, 388
TspDTI ATGAA 1 cut(s) 313
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XhoI CTCGAG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 82
XspI CTAG 1 cut(s) 479
ZraI GACGTC 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.