Rw4G012710

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002231
Physical Location & Seq
Forward (+)
29899597 .. 29908677
9081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012710.1

Sequence Viewer

Length: 3291 bp
ATGAATGAAGAGGAAGTGGAACCTCCACTCGGGGTTCTATTGGGTCGAAAGCGCGGGAAAACGAAGTCCGCCAAGAGAGCAAGCCAAGTAACACCGCACAGCATAGAGCCACTTCAAATCGAACCAGAGCCTGTCCCGATCGATGCGGAGCCAATTCAAATTGAGTCAGAGCCAGTTGTAATTGAGCCAGAGAACTCAAGGAATGGCGCTAGGGTTAGTGTTTCGATGTTCGATGACTCGGTGGAGAATCACTTTAGAGTCATGCACACGATTTCTGAGCTCTGTGGCGACGCCGAAGACGAGCCACTCCCGGTGAGCGACATTCAACGCTTATCATCCTCCACCACTTTCTTGAGGGAATGGGCAGATTTTAAGTATGAACCTAGAGATGTTCGATTCACTTGCGAAGCAGAAAGCTCTGATGAGGAGGATGTTGTCAGTAGGATAAACTTGCGGCAGTTTTCTTCTGCAACTGTTCCACAGAACGAGGCGGGGAACACATCTTCTCCAGAGTTGAGACAAGACTATGTGATGTATGTTGGAGGGCCTGTTTGGGCATTGGATTGGTGTCCTAGAGTTCATGAAAGTCACGACCGTCATGCAAAGTGCGAGTTTATTGCCATTGCTGCTCATCCCCCTGGATCTTCTTATCACAAGTTAGGTGAGCCACTTACTGGCAGAGACAAACCAAAAGGAGGAAATAAAAGGAGTAGAGCCACAGAAAAATGCACTGAACAAAAAAGGGGAAGACCCAGAAAGAAGCCATTAGAAGAATCTGTTGGCAAAGAGGCCACAGAAGAAAAACTGTCTGAGCCAAAAAGGCCGAGAGGAAGACCTAGAAAGAAGCCAATAGAAGAATCTATTGACAAAGAGGGCAAAGAAGAGAAATCAACTCAACCAAAGAGGTCTAGAGGAAGACCTAGAAATAAATCAATAGAAGAATCTGTTGACAACTTGGATGGTCTCACCAATTGTGATGAGGTTCTTGCTATTGATTATCCAAAGGAATCATCTGAGTTGCATTCTATGGATTGTCTTCCTGCAAATACTCAAGGAAATTTTGTACAAGAATATCATGGCAAAAAGCAACTAAGTTATGGGCAGAGTCAACCGAAGAAGCCTAGAGGAAGACCCAGAAAGAAGCCAATAAAAGAACCTGTTGACAATTTAGATGGGGGCAACAATAATTTTGAGGTTCTTGCCATTGAATACCAAAAGGAATCACTGGAGTTGCATTCTACGGATTGTCTTCTTGCAAATACTCAAGTGGACGCTGTACAAGAAACAAGTTACAAGCAGACTCGATCAAAGAGGCCTAGAGGAATACATGGAAAGAAGCCAATAGAAGAATCTGTTGAAAACTTGGATGACAGCAGCAATACGGGTGAGGCTCTTGATGATCAATATCCAAAGGAATTATTGGAGTTGGAGGGCAGGGATTGCGCTCCTGAAAATACTCAAGGAGATGAGGTACAAGATGATAGTGGTAAGAAAAGAAAAACGTACAGAAAGACTCGAGCAAAGATTCCTAGAGAAAAACCTGGGGAGAAGCTAGTAGAATATTCTGTTGATAACTTGCATGGGAGCAGCCATTATGCGGAGGCTCTTGGAGTCCAATATCTCAAGGAAACATCAGAGTTGCATGCTATGGATTGTGTTCCTGCATTTACTCAGGCGCATGATGTACAAGAAGCTCTTAATGAAAAACTTGAAAGTTCCGGACCGGGTCCTCAAACAAATAATAATGTTTCAAATAATGGTTATGCTGAAATTGGCTCAATTAGATGTTCAGTTCCAAAGGATGTAGCATTGCCAAGAATCATGTTCTGCCTAGCTCACCATGGAAAGGTTGCTTGGGATGTGAAATGGCGTCCTCTTAATGAACATGATTCCAGATGCAAGCATCGAATGGGCTATCTTGCTGTTCTGTTGGGGAACGGGTCTCTGGAAGTGTGGGAGGTTCCTGTTCCTCGTGCAATTGAAGTTATTTATTCTTCTTCATCTGGAGATGGCACTGATCCTCGCTTTGTAAAATTGGCACCAGTATTTAGATGCTCAATGTTGAAGTCTGGTGACAAAAAAAGCATCCCTCTTACTGTGGAATGGTCAGCTTCACCACCTCACGATTACTTAATTGCCGGATGCCATGATGGAACTGTTGCTATGTGGAAGTTTTCTGCTAGTAATGTATCTCAAGATACAAGGCCTTTACTTTGTTTCAGCGCAGATACAAATCCTATTAGGGCGCTTTCTTGGGCCCCAGCTCAAAGCAATTCAGACAATGCAAATGTTATAGCTACTGCTGGACATGGAGGCCTAAAATTTTGGGACCTACGTGATCCATTCCGTCCCTTGTGGGACATCGATCATATACCAAGGTTCATATATAGTCTGGATTGGATTCCAGATCCAAGGTGTGTTCTTCTCTCCTTTGATGATGGAACAATGAAACTTCTCAGCTTGACAAAGGCTGCCTCTGATGCCCCTGTAACAGGAAAACCCTTTGCTGGAACAAAACAACAGGGCCTGCACAATCTTGGTTGTTTGCCTTTTGCTATCTGGAGTGTTCAAGTATCAAGACTAACAGGTATGGTTGCATATTGTGGTGCAGATGGAACTGTTCTGCGCTTCCAACTTACGTCCAATGCTGTGGAGAAAGATGCTACACGAAACCGGGCCCCACATTTTCTGTGCGTGTCACTAACTGAAGAGGACTCTGTTGTGACCATCAATACCCCAGTACCACATAATCCTTTCCCGTTGAAAACATCTCGTAAAGCTGAGCCAAACAAAGTGAAGAGAGAACATGATAAGATGACAACTACATCACAAGATCAAGTTTTAGCTCTTTGTTACGGTGATGATCCGGGTATTGAATTAGAATCTGGGAAAGAAGCAGCATTTCTTAGAAGCAAAACACTCAAATCTGCGGATGACCAAGCATTAGTATGCATGGATCAAGAGCCATTTAATACCCAAGACGAAGAAATTGGAGAAAAGGGTACATCTCTTAAAGGCATTGTAAAACAGAAATCCAAGTCTAGTAAGAAAAATACAGAGGATGAACAAGAACTGGAAGGCAGAGATGAAGAGCTGATTAATACGCAGAGAGAAAAAACTGGGACCGAGTATGAAGTTTTTCCTTCCAAGCAAGTAGCAATGCACCGAGTGAGATGGAACATGAACAAGGGTAGTGAGAGATGGTTATGCTATGGAGGGGCAGCTGGAATAGTACGTTGTCAAGAGATTGTTTTGTCTGACATTGATATGAAATGGGCGAGGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook

Protein Analysis

1096

Amino Acids

122.56

Weight (kDa)

5.98

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 699 - 825 7.9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2038
AccB7I CCANNNNNTGG 1 cut(s) 674
AccII CGCG 1 cut(s) 54
AccIII TCCGGA 1 cut(s) 1718
AciI CCGC 8 cut(s) 54, 69, 95, 146, 454, 491, 1598, 2930
AclWI GGATC 6 cut(s) 649, 2012, 2333, 2402, 2858, 2964
AcsI RAATTY 2 cut(s) 1057, 2321
AcuI CTGAAG 1 cut(s) 2727
AcyI GRCGYC 2 cut(s) 291, 1870
AdeI CACNNNGTG 1 cut(s) 3169
AfaI GTAC 8 cut(s) 1065, 1278, 1473, 1505, 1686, 2742, 3004, 3234
AfiI CCNNNNNNNGG 7 cut(s) 29, 674, 695, 1597, 1846, 2492, 2507
AjnI CCWGG 2 cut(s) 637, 1540
AjuI GAANNNNNNNTTGG 4 cut(s) 762, 794, 1837, 1869
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 4 cut(s) 511, 675, 968, 1947
AlwI GGATC 6 cut(s) 649, 2012, 2333, 2402, 2858, 2964
AlwNI CAGNNNCTG 2 cut(s) 131, 2527
Ama87I CYCGRG 2 cut(s) 29, 1515
Aor13HI TCCGGA 1 cut(s) 1718
AoxI GGCC 9 cut(s) 545, 789, 821, 1313, 2204, 2256, 2314, 2524, 2676
ApaI GGGCCC 2 cut(s) 2260, 2680
ApeKI GCWGC 6 cut(s) 626, 1374, 1587, 2471, 2897, 3221
ApoI RAATTY 2 cut(s) 1057, 2321
AseI ATTAAT 1 cut(s) 3099
Asp700I GAANNNNTTC 1 cut(s) 3138
AspLEI GCGC 7 cut(s) 54, 209, 1445, 1678, 2225, 2248, 2628
AsuC2I CCSGG 4 cut(s) 311, 1725, 2675, 2868
AsuHPI GGTGA 8 cut(s) 325, 674, 958, 1397, 1829, 2084, 2106, 2870
AvaI CYCGRG 2 cut(s) 29, 1515
AvaII GGWCC 4 cut(s) 1721, 1727, 2329, 3123
BaeGI GKGCMC 2 cut(s) 2260, 2680
BaeI ACNNNNGTAYC 2 cut(s) 2724, 2757
BanI GGYRCC 1 cut(s) 2038
BanII GRGCYC 3 cut(s) 282, 2260, 2680
BauI CACGAG 1 cut(s) 1971
BbsI GAAGAC 7 cut(s) 303, 754, 838, 922, 1028, 1135, 1241
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 6 cut(s) 613, 1386, 1599, 2458, 2909, 3233
BccI CCATC 9 cut(s) 953, 1166, 2003, 2144, 2432, 2606, 2735, 3168, 3195
BcgI CGANNNNNNTGC 6 cut(s) 384, 418, 581, 599, 615, 633
BciT130I CCWGG 2 cut(s) 639, 1542
BclI TGATCA 1 cut(s) 1399
BcnI CCSGG 4 cut(s) 311, 1725, 2675, 2868
BcoDI GTCTC 4 cut(s) 511, 675, 968, 1947
BfoI RGCGCY 2 cut(s) 210, 2249
BglI GCCNNNNNGGC 1 cut(s) 820
BisI GCNGC 7 cut(s) 455, 627, 1375, 1588, 2472, 2898, 3222
BlpI GCTNAGC 1 cut(s) 2781
BlsI GCNGC 7 cut(s) 456, 628, 1376, 1589, 2473, 2899, 3223
Bme1390I CCNGG 6 cut(s) 311, 639, 1542, 1725, 2675, 2868
Bme18I GGWCC 4 cut(s) 1721, 1727, 2329, 3123
BmeT110I CYCGRG 2 cut(s) 29, 1515
BmrFI CCNGG 6 cut(s) 311, 639, 1542, 1725, 2675, 2868
BmrI ACTGGG 2 cut(s) 2732, 3129
BmsI GCATC 8 cut(s) 133, 1886, 1912, 2042, 2094, 2132, 2470, 2650
BmuI ACTGGG 2 cut(s) 2732, 3129
BpiI GAAGAC 7 cut(s) 303, 754, 838, 922, 1028, 1135, 1241
BpmI CTGGAG 4 cut(s) 492, 1247, 2025, 2581
Bpu1102I GCTNAGC 1 cut(s) 2781
BpuEI CTTGAG 7 cut(s) 181, 373, 1035, 1248, 1443, 1607, 2178
BpuMI CCSGG 4 cut(s) 311, 1725, 2675, 2868
Bsa29I ATCGAT 2 cut(s) 141, 2364
BsaAI YACGTR 1 cut(s) 2336
BsaBI GATNNNNATC 2 cut(s) 1404, 2232
BsaHI GRCGYC 2 cut(s) 291, 1870
BsaI GGTCTC 2 cut(s) 968, 1947
BsaJI CCNNGG 5 cut(s) 637, 1541, 1840, 2375, 2411
BsaWI WCCGGW 1 cut(s) 1718
BsaXI ACNNNNNCTCC 6 cut(s) 490, 520, 534, 564, 1455, 1485
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 674, 695, 1597, 1846, 2492, 2507
Bse1I ACTGG 7 cut(s) 173, 679, 1230, 2042, 2738, 3078, 3124
Bse3DI GCAATG 3 cut(s) 621, 1808, 3165
Bse8I GATNNNNATC 2 cut(s) 1404, 2232
BseAI TCCGGA 1 cut(s) 1718
BseBI CCWGG 2 cut(s) 639, 1542
BseCI ATCGAT 2 cut(s) 141, 2364
BseDI CCNNGG 5 cut(s) 637, 1541, 1840, 2375, 2411
BseJI GATNNNNATC 2 cut(s) 1404, 2232
BseLI CCNNNNNNNGG 7 cut(s) 29, 674, 695, 1597, 1846, 2492, 2507
BseMI GCAATG 3 cut(s) 621, 1808, 3165
BseMII CTCAG 6 cut(s) 267, 801, 1005, 1685, 2470, 2772
BseNI ACTGG 7 cut(s) 173, 679, 1230, 2042, 2738, 3078, 3124
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 2 cut(s) 2260, 2680
BseXI GCAGC 6 cut(s) 613, 1386, 1599, 2458, 2909, 3233
BseYI CCCAGC 1 cut(s) 2260
BsgI GTGCAG 2 cut(s) 2513, 2628
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 2 cut(s) 141, 595
BshFI GGCC 9 cut(s) 547, 791, 823, 1315, 2206, 2258, 2316, 2526, 2678
BshNI GGYRCC 1 cut(s) 2038
BshVI ATCGAT 2 cut(s) 141, 2364
BsiEI CGRYCG 2 cut(s) 141, 595
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 2 cut(s) 29, 1515
BsiSI CCGG 6 cut(s) 311, 1719, 1724, 2139, 2674, 2867
BslFI GGGAC 5 cut(s) 119, 2334, 2342, 2372, 3136
BslI CCNNNNNNNGG 7 cut(s) 29, 674, 695, 1597, 1846, 2492, 2507
BsmAI GTCTC 4 cut(s) 511, 675, 968, 1947
BsmFI GGGAC 5 cut(s) 119, 2334, 2342, 2372, 3136
BsmI GAATGC 2 cut(s) 1021, 1234
BsnI GGCC 9 cut(s) 547, 791, 823, 1315, 2206, 2258, 2316, 2526, 2678
Bso31I GGTCTC 2 cut(s) 968, 1947
BsoBI CYCGRG 2 cut(s) 29, 1515
Bsp120I GGGCCC 2 cut(s) 2256, 2676
Bsp1286I GDGCHC 3 cut(s) 282, 2260, 2680
Bsp13I TCCGGA 1 cut(s) 1718
Bsp1407I TGTACA 3 cut(s) 1063, 1276, 1684
Bsp1720I GCTNAGC 1 cut(s) 2781
Bsp19I CCATGG 1 cut(s) 1840
BspACI CCGC 8 cut(s) 54, 69, 95, 146, 454, 491, 1598, 2930
BspANI GGCC 9 cut(s) 547, 791, 823, 1315, 2206, 2258, 2316, 2526, 2678
BspCNI CTCAG 6 cut(s) 268, 802, 1006, 1684, 2469, 2773
BspDI ATCGAT 2 cut(s) 141, 2364
BspEI TCCGGA 1 cut(s) 1718
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 580
BspPI GGATC 6 cut(s) 649, 2012, 2333, 2402, 2858, 2964
BspQI GCTCTTC 1 cut(s) 3084
BspT107I GGYRCC 1 cut(s) 2038
BspTNI GGTCTC 2 cut(s) 968, 1947
BsrDI GCAATG 3 cut(s) 621, 1808, 3165
BsrGI TGTACA 3 cut(s) 1063, 1276, 1684
BsrI ACTGG 7 cut(s) 173, 679, 1230, 2042, 2738, 3078, 3124
BssECI CCNNGG 5 cut(s) 637, 1541, 1840, 2375, 2411
BssNI GRCGYC 2 cut(s) 291, 1870
BssSI CACGAG 1 cut(s) 1971
BssT1I CCWWGG 3 cut(s) 1840, 2375, 2411
Bst2BI CACGAG 1 cut(s) 1971
Bst2UI CCWGG 2 cut(s) 639, 1542
Bst4CI ACNGT 7 cut(s) 475, 596, 807, 2098, 2158, 2620, 2858
Bst6I CTCTTC 5 cut(s) 3, 876, 2703, 2792, 3084
BstACI GRCGYC 2 cut(s) 291, 1870
BstAPI GCANNNNNTGC 1 cut(s) 1440
BstAUI TGTACA 3 cut(s) 1063, 1276, 1684
BstBAI YACGTR 1 cut(s) 2336
BstC8I GCNNGC 4 cut(s) 82, 1644, 1901, 2528
BstDEI CTNAG 8 cut(s) 276, 810, 1014, 1091, 1671, 2456, 2781, 2906
BstDSI CCRYGG 1 cut(s) 1840
BstENI CCTNNNNNAGG 1 cut(s) 2490
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 2 cut(s) 210, 2249
BstHHI GCGC 7 cut(s) 54, 209, 1445, 1678, 2225, 2248, 2628
BstMAI GTCTC 4 cut(s) 511, 675, 968, 1947
BstMCI CGRYCG 2 cut(s) 141, 595
BstMWI GCNNNNNNNGC 5 cut(s) 77, 626, 820, 1440, 2480
BstNI CCWGG 2 cut(s) 639, 1542
BstNSI RCATGY 1 cut(s) 1646
BstSCI CCNGG 6 cut(s) 309, 637, 1540, 1723, 2673, 2866
BstSLI GKGCMC 2 cut(s) 2260, 2680
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 6 cut(s) 613, 1386, 1599, 2458, 2909, 3233
BstV2I GAAGAC 7 cut(s) 303, 754, 838, 922, 1028, 1135, 1241
BstX2I RGATCY 2 cut(s) 641, 2407
BstXI CCANNNNNNTGG 1 cut(s) 2650
BstYI RGATCY 2 cut(s) 641, 2407
Bsu15I ATCGAT 2 cut(s) 141, 2364
BsuRI GGCC 9 cut(s) 547, 791, 823, 1315, 2206, 2258, 2316, 2526, 2678
BsuTUI ATCGAT 2 cut(s) 141, 2364
BtgI CCRYGG 1 cut(s) 1840
BtsIMutI CAGTG 3 cut(s) 729, 1223, 2013
Cac8I GCNNGC 4 cut(s) 82, 1644, 1901, 2528
CaiI CAGNNNCTG 2 cut(s) 131, 2527
CciI TCATGA 1 cut(s) 580
CfoI GCGC 7 cut(s) 54, 209, 1445, 1678, 2225, 2248, 2628
ClaI ATCGAT 2 cut(s) 141, 2364
CpoI CGGWCCG 1 cut(s) 1721
CseI GACGC 3 cut(s) 299, 1280, 1859
Csp6I GTAC 8 cut(s) 1064, 1277, 1472, 1504, 1685, 2741, 3003, 3233
CspI CGGWCCG 1 cut(s) 1721
CviQI GTAC 8 cut(s) 1064, 1277, 1472, 1504, 1685, 2741, 3003, 3233
DdeI CTNAG 8 cut(s) 276, 810, 1014, 1091, 1671, 2456, 2781, 2906
DraIII CACNNNGTG 1 cut(s) 3169
Eam1104I CTCTTC 5 cut(s) 3, 876, 2703, 2792, 3084
EarI CTCTTC 5 cut(s) 3, 876, 2703, 2792, 3084
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 1840, 2375, 2411
Eco147I AGGCCT 3 cut(s) 1315, 2206, 2316
Eco24I GRGCYC 3 cut(s) 282, 2260, 2680
Eco31I GGTCTC 2 cut(s) 968, 1947
Eco47I GGWCC 4 cut(s) 1721, 1727, 2329, 3123
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 2727
Eco88I CYCGRG 2 cut(s) 29, 1515
EcoICRI GAGCTC 1 cut(s) 280
EcoNI CCTNNNNNAGG 1 cut(s) 2490
EcoO109I RGGNCCY 6 cut(s) 545, 1727, 2257, 2329, 2524, 2677
EcoRII CCWGG 2 cut(s) 637, 1540
EcoT14I CCWWGG 3 cut(s) 1840, 2375, 2411
EcoT22I ATGCAT 1 cut(s) 2954
EcoT38I GRGCYC 3 cut(s) 282, 2260, 2680
ErhI CCWWGG 3 cut(s) 1840, 2375, 2411
FalI AAGNNNNNCTT 2 cut(s) 1680, 1712
FaqI GGGAC 5 cut(s) 119, 2334, 2342, 2372, 3136
FauI CCCGC 2 cut(s) 47, 484
FbaI TGATCA 1 cut(s) 1399
Fnu4HI GCNGC 7 cut(s) 455, 627, 1375, 1588, 2472, 2898, 3222
FriOI GRGCYC 3 cut(s) 282, 2260, 2680
Fsp4HI GCNGC 7 cut(s) 455, 627, 1375, 1588, 2472, 2898, 3222
GlaI GCGC 7 cut(s) 53, 208, 1444, 1677, 2224, 2247, 2627
GluI GCNGC 7 cut(s) 455, 627, 1375, 1588, 2472, 2898, 3222
GsaI CCCAGC 1 cut(s) 2264
GsuI CTGGAG 4 cut(s) 492, 1247, 2025, 2581
HaeII RGCGCY 2 cut(s) 210, 2249
HaeIII GGCC 9 cut(s) 547, 791, 823, 1315, 2206, 2258, 2316, 2526, 2678
HapII CCGG 6 cut(s) 311, 1719, 1724, 2139, 2674, 2867
HgaI GACGC 3 cut(s) 299, 1280, 1859
HhaI GCGC 7 cut(s) 54, 209, 1445, 1678, 2225, 2248, 2628
Hin1I GRCGYC 2 cut(s) 291, 1870
Hin6I GCGC 7 cut(s) 52, 207, 1443, 1676, 2223, 2246, 2626
HinP1I GCGC 7 cut(s) 52, 207, 1443, 1676, 2223, 2246, 2626
HincII GTYRAC 3 cut(s) 949, 1109, 1162
HindII GTYRAC 3 cut(s) 949, 1109, 1162
HpaII CCGG 6 cut(s) 311, 1719, 1724, 2139, 2674, 2867
HphI GGTGA 8 cut(s) 325, 674, 958, 1397, 1829, 2084, 2106, 2870
Hpy166II GTNNAC 4 cut(s) 949, 1109, 1162, 1270
Hpy188I TCNGA 9 cut(s) 169, 277, 421, 811, 1015, 1636, 2278, 2479, 3259
Hpy8I GTNNAC 4 cut(s) 949, 1109, 1162, 1270
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 3071, 3153
HpyCH4III ACNGT 7 cut(s) 475, 596, 807, 2098, 2158, 2620, 2858
HpyCH4IV ACGT 4 cut(s) 1502, 2335, 2639, 3235
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 626, 820, 1440, 2480
HpyF3I CTNAG 8 cut(s) 276, 810, 1014, 1091, 1671, 2456, 2781, 2906
HpySE526I ACGT 4 cut(s) 1502, 2335, 2639, 3235
Hsp92I GRCGYC 2 cut(s) 291, 1870
HspAI GCGC 7 cut(s) 52, 207, 1443, 1676, 2223, 2246, 2626
Kpn2I TCCGGA 1 cut(s) 1718
Ksp22I TGATCA 1 cut(s) 1399
LguI GCTCTTC 1 cut(s) 3084
LmnI GCTCC 3 cut(s) 148, 1450, 1584
Lsp1109I GCAGC 6 cut(s) 613, 1386, 1599, 2458, 2909, 3233
LweI GCATC 8 cut(s) 133, 1886, 1912, 2042, 2094, 2132, 2470, 2650
MaeII ACGT 4 cut(s) 1502, 2335, 2639, 3235
MaeIII GTNAC 8 cut(s) 88, 587, 1289, 2072, 2488, 2697, 2722, 2852
MfeI CAATTG 2 cut(s) 970, 1977
MflI RGATCY 2 cut(s) 641, 2407
MhlI GDGCHC 3 cut(s) 282, 2260, 2680
MlyI GAGTC 8 cut(s) 173, 230, 267, 1114, 1294, 1507, 1620, 2708
MmeI TCCRAC 3 cut(s) 520, 1407, 2656
Mph1103I ATGCAT 1 cut(s) 2954
MroI TCCGGA 1 cut(s) 1718
MroXI GAANNNNTTC 1 cut(s) 3138
MseI TTAA 7 cut(s) 372, 1698, 1878, 2132, 2970, 3012, 3099
MslI CAYNNNNRTG 2 cut(s) 2947, 3266
MspA1I CMGCKG 1 cut(s) 3224
MspI CCGG 6 cut(s) 311, 1719, 1724, 2139, 2674, 2867
MspR9I CCNGG 6 cut(s) 311, 639, 1542, 1725, 2675, 2868
MunI CAATTG 2 cut(s) 970, 1977
Mva1269I GAATGC 2 cut(s) 1021, 1234
MvaI CCWGG 2 cut(s) 639, 1542
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 5 cut(s) 77, 626, 820, 1440, 2480
NciI CCSGG 4 cut(s) 311, 1725, 2675, 2868
NcoI CCATGG 1 cut(s) 1840
NmeAIII GCCGAG 1 cut(s) 849
NmuCI GTSAC 4 cut(s) 587, 2072, 2697, 2722
NsiI ATGCAT 1 cut(s) 2954
NspI RCATGY 1 cut(s) 1646
PaeI GCATGC 1 cut(s) 1646
PaeR7I CTCGAG 1 cut(s) 1515
PagI TCATGA 1 cut(s) 580
PceI AGGCCT 3 cut(s) 1315, 2206, 2316
PciSI GCTCTTC 1 cut(s) 3084
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 2 cut(s) 1021, 1234
PdmI GAANNNNTTC 1 cut(s) 3138
PflFI GACNNNGTC 1 cut(s) 1725
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 7 cut(s) 456, 628, 1376, 1589, 2473, 2899, 3223
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 8 cut(s) 172, 230, 266, 1113, 1294, 1507, 1619, 2708
PpsI GAGTC 8 cut(s) 172, 230, 266, 1113, 1294, 1507, 1619, 2708
Ppu21I YACGTR 1 cut(s) 2336
PpuMI RGGWCCY 2 cut(s) 1727, 2329
PshBI ATTAAT 1 cut(s) 3099
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 2 cut(s) 1727, 2329
Psp6I CCWGG 2 cut(s) 637, 1540
PspFI CCCAGC 1 cut(s) 2260
PspGI CCWGG 2 cut(s) 637, 1540
PspOMI GGGCCC 2 cut(s) 2256, 2676
PspPPI RGGWCCY 2 cut(s) 1727, 2329
PspXI VCTCGAGB 1 cut(s) 1515
PstNI CAGNNNCTG 2 cut(s) 131, 2527
PsuI RGATCY 2 cut(s) 641, 2407
PsyI GACNNNGTC 1 cut(s) 1725
PvuI CGATCG 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 3224
RsaI GTAC 8 cut(s) 1065, 1278, 1473, 1505, 1686, 2742, 3004, 3234
RsaNI GTAC 8 cut(s) 1064, 1277, 1472, 1504, 1685, 2741, 3003, 3233
RseI CAYNNNNRTG 2 cut(s) 2947, 3266
Rsr2I CGGWCCG 1 cut(s) 1721
RsrII CGGWCCG 1 cut(s) 1721
SacI GAGCTC 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 3084
SaqAI TTAA 7 cut(s) 372, 1698, 1878, 2132, 2970, 3012, 3099
SatI GCNGC 7 cut(s) 455, 627, 1375, 1588, 2472, 2898, 3222
SchI GAGTC 8 cut(s) 173, 230, 267, 1114, 1294, 1507, 1620, 2708
ScrFI CCNGG 6 cut(s) 311, 639, 1542, 1725, 2675, 2868
SduI GDGCHC 3 cut(s) 282, 2260, 2680
SfaNI GCATC 8 cut(s) 133, 1886, 1912, 2042, 2094, 2132, 2470, 2650
Sfr274I CTCGAG 1 cut(s) 1515
SinI GGWCC 4 cut(s) 1721, 1727, 2329, 3123
SlaI CTCGAG 1 cut(s) 1515
SmiMI CAYNNNNRTG 2 cut(s) 2947, 3266
SmlI CTYRAG 8 cut(s) 196, 352, 1050, 1263, 1458, 1515, 1622, 2193
SmoI CTYRAG 8 cut(s) 196, 352, 1050, 1263, 1458, 1515, 1622, 2193
SphI GCATGC 1 cut(s) 1646
SseBI AGGCCT 3 cut(s) 1315, 2206, 2316
SsiI CCGC 8 cut(s) 54, 69, 95, 146, 454, 491, 1598, 2930
SspI AATATT 1 cut(s) 1562
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 3 cut(s) 1315, 2206, 2316
StyD4I CCNGG 6 cut(s) 309, 637, 1540, 1723, 2673, 2866
StyI CCWWGG 3 cut(s) 1840, 2375, 2411
TaaI ACNGT 7 cut(s) 475, 596, 807, 2098, 2158, 2620, 2858
TaiI ACGT 4 cut(s) 1505, 2338, 2642, 3238
TaqII GACCGA 1 cut(s) 3140
TatI WGTACW 3 cut(s) 1063, 1276, 1684
TauI GCSGC 1 cut(s) 457
Tru1I TTAA 7 cut(s) 372, 1698, 1878, 2132, 2970, 3012, 3099
Tru9I TTAA 7 cut(s) 372, 1698, 1878, 2132, 2970, 3012, 3099
TscAI CASTG 3 cut(s) 736, 1230, 2020
TseFI GTSAC 4 cut(s) 587, 2072, 2697, 2722
TseI GCWGC 6 cut(s) 626, 1374, 1587, 2471, 2897, 3221
Tsp45I GTSAC 4 cut(s) 587, 2072, 2697, 2722
TspGWI ACGGA 2 cut(s) 1256, 2336
TspRI CASTG 3 cut(s) 736, 1230, 2020
Tth111I GACNNNGTC 1 cut(s) 1725
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 4 cut(s) 1721, 1727, 2329, 3123
VspI ATTAAT 1 cut(s) 3099
XagI CCTNNNNNAGG 1 cut(s) 2490
XapI RAATTY 2 cut(s) 1057, 2321
XbaI TCTAGA 1 cut(s) 908
XceI RCATGY 1 cut(s) 1646
XhoI CTCGAG 1 cut(s) 1515
XmnI GAANNNNTTC 1 cut(s) 3138
Zsp2I ATGCAT 1 cut(s) 2954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.