MD09G1197000.v1.1

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001575
Physical Location & Seq
Reverse (-)
17939682 .. 17940968
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1197000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAAGTAGGACCATGGGGAGGAAATGGTGGAACTCCTTGGGACGACGGCATCTACAATGGCGTGAGAGAAATCACGCTTGTTTATGGCGAATGCATCGACTCCATCACCGTGGTGTATGATAAAAATGGCAAGCCCTTCAAAGCTGAAATGCATGGAGGTCACGGAGGCACTCAAACCGCGGAGATAAAGCTGCTGTACCCCGATGAGTACATAGTCAGTGTGACCGGGCACTATTGTACGATGGTGGATGCCGGCATCTATTCCCCTATCATCCGTTCGCTTAAATTTCAGAGCAATCGAAGAACATTCGGACCATTTGGAAGGGATCAAGGCACACCGTTTACTTACACTCTGGATGGAGGCAAAATTGTTGGCTTGAAGGGAAGAGATGGTTTATACATAGATGCCATTGGGTTTCATGTATCCCCTGCCCCAGCAAGACTCTCTGACAGAGTTCAGAAGAGTTTCAGAAGGCTCGGGTGCTCGGTTACCTGCGGAGCCCAGATAAACTGCAACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD Jacalin

Protein Analysis

175

Amino Acids

18.95

Weight (kDa)

6.89

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 3 - 141 1e-42 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 503
AccII CGCG 1 cut(s) 182
AciI CCGC 3 cut(s) 180, 182, 498
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 3 cut(s) 200, 212, 241
AfiI CCNNNNNNNGG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 142, 382
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 2 cut(s) 146, 193
AluI AGCT 2 cut(s) 146, 193
Alw21I GWGCWC 1 cut(s) 488
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 287
Asp700I GAANNNNTTC 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 11, 314
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 2 cut(s) 11, 314
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 180
BccI CCATC 4 cut(s) 113, 238, 353, 386
BceAI ACGGC 1 cut(s) 64
BciVI GTATCC 1 cut(s) 436
BcnI CCSGG 1 cut(s) 229
BfaI CTAG 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 503
BfuI GTATCC 1 cut(s) 436
BisI GCNGC 1 cut(s) 194
BlsI GCNGC 1 cut(s) 195
Bme1390I CCNGG 1 cut(s) 229
Bme18I GGWCC 2 cut(s) 11, 314
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 11, 314
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 229
BmsI GCATC 5 cut(s) 60, 105, 241, 267, 397
BpuMI CCSGG 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 14, 38, 111, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse118I RCCGGY 1 cut(s) 254
BseDI CCNNGG 4 cut(s) 14, 38, 111, 180
BseGI GGATG 3 cut(s) 256, 273, 364
BseLI CCNNNNNNNGG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 1 cut(s) 180
BseYI CCCAGC 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 228, 255
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 234, 488, 505
Bsp143I GATC 1 cut(s) 328
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 3 cut(s) 180, 182, 498
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 502
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 14, 38, 111, 180
BssMI GATC 1 cut(s) 328
BssT1I CCWWGG 2 cut(s) 14, 38
Bst4CI ACNGT 2 cut(s) 112, 342
Bst6I CTCTTC 2 cut(s) 382, 458
BstC8I GCNNGC 2 cut(s) 134, 256
BstDSI CCRYGG 3 cut(s) 14, 111, 180
BstEII GGTNACC 1 cut(s) 490
BstF5I GGATG 3 cut(s) 256, 273, 364
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 1 cut(s) 331
BstMBI GATC 1 cut(s) 328
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 227
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 180
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuI GTATCC 1 cut(s) 436
BtgI CCRYGG 3 cut(s) 14, 111, 180
BtsCI GGATG 3 cut(s) 256, 273, 364
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 2 cut(s) 134, 256
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 11, 314
Cfr42I CCGCGG 1 cut(s) 183
Csp6I GTAC 3 cut(s) 199, 211, 240
CviAII CATG 3 cut(s) 15, 155, 422
CviJI RGCY 6 cut(s) 136, 146, 193, 378, 478, 503
CviKI_1 RGCY 6 cut(s) 136, 146, 193, 378, 478, 503
CviQI GTAC 3 cut(s) 199, 211, 240
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 382, 458
EarI CTCTTC 2 cut(s) 382, 458
Eco130I CCWWGG 2 cut(s) 14, 38
Eco24I GRGCYC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 11, 314
Eco88I CYCGRG 1 cut(s) 479
Eco91I GGTNACC 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 490
EcoT14I CCWWGG 2 cut(s) 14, 38
EcoT22I ATGCAT 2 cut(s) 98, 156
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 2 cut(s) 14, 38
FaeI CATG 3 cut(s) 18, 158, 425
FaiI YATR 8 cut(s) 16, 87, 120, 156, 215, 400, 404, 423
FaqI GGGAC 1 cut(s) 56
FatI CATG 3 cut(s) 14, 154, 421
Fnu4HI GCNGC 1 cut(s) 194
FokI GGATG 3 cut(s) 260, 263, 371
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 1 cut(s) 194
FspBI CTAG 1 cut(s) 523
GluI GCNGC 1 cut(s) 194
GsaI CCCAGC 1 cut(s) 440
HapII CCGG 2 cut(s) 228, 255
Hin1II CATG 3 cut(s) 18, 158, 425
HinfI GANTC 2 cut(s) 101, 444
HpaII CCGG 2 cut(s) 228, 255
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 5 cut(s) 294, 314, 451, 462, 473
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 1 cut(s) 345
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 4 cut(s) 148, 318, 376, 468
HpyCH4III ACNGT 2 cut(s) 112, 342
HpyCH4V TGCA 3 cut(s) 96, 154, 516
Hsp92II CATG 3 cut(s) 18, 158, 425
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
KspI CCGCGG 1 cut(s) 183
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 500
LpnPI CCDG 7 cut(s) 241, 268, 341, 444, 450, 508, 518
Lsp1109I GCAGC 1 cut(s) 180
LweI GCATC 5 cut(s) 60, 105, 241, 267, 397
MaeI CTAG 1 cut(s) 523
MaeIII GTNAC 3 cut(s) 161, 223, 490
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 3 cut(s) 315, 399, 475
MhlI GDGCHC 3 cut(s) 234, 488, 505
MluCI AATT 2 cut(s) 287, 369
MlyI GAGTC 2 cut(s) 95, 438
MnlI CCTC 4 cut(s) 14, 152, 161, 356
Mph1103I ATGCAT 2 cut(s) 98, 156
MroNI GCCGGC 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 2 cut(s) 110, 113
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 2 cut(s) 228, 255
MspR9I CCNGG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 98
MvnI CGCG 1 cut(s) 182
NaeI GCCGGC 1 cut(s) 256
NciI CCSGG 1 cut(s) 229
NcoI CCATGG 1 cut(s) 14
NdeII GATC 1 cut(s) 328
NgoMIV GCCGGC 1 cut(s) 254
NlaIII CATG 3 cut(s) 18, 158, 425
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 161, 223
NsiI ATGCAT 2 cut(s) 98, 156
OliI CACNNNNGTG 1 cut(s) 113
PctI GAATGC 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 195
PleI GAGTC 2 cut(s) 95, 438
PpsI GAGTC 2 cut(s) 95, 438
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 2 cut(s) 11, 314
RsaI GTAC 3 cut(s) 200, 212, 241
RsaNI GTAC 3 cut(s) 199, 211, 240
RseI CAYNNNNRTG 2 cut(s) 110, 113
SacII CCGCGG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 194
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 2 cut(s) 11, 314
SchI GAGTC 2 cut(s) 95, 438
ScrFI CCNGG 1 cut(s) 229
SduI GDGCHC 3 cut(s) 234, 488, 505
SetI ASST 4 cut(s) 148, 163, 195, 497
SfaNI GCATC 5 cut(s) 60, 105, 241, 267, 397
Sfr303I CCGCGG 1 cut(s) 183
SgrBI CCGCGG 1 cut(s) 183
SinI GGWCC 2 cut(s) 11, 314
SmiMI CAYNNNNRTG 2 cut(s) 110, 113
Sse9I AATT 2 cut(s) 287, 369
SsiI CCGC 3 cut(s) 180, 182, 498
SspMI CTAG 1 cut(s) 523
StyD4I CCNGG 1 cut(s) 227
StyI CCWWGG 2 cut(s) 14, 38
TaaI ACNGT 2 cut(s) 112, 342
TaqI TCGA 2 cut(s) 99, 301
TasI AATT 2 cut(s) 287, 369
TatI WGTACW 1 cut(s) 210
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 161, 223
TseI GCWGC 1 cut(s) 193
Tsp45I GTSAC 2 cut(s) 161, 223
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 2 cut(s) 180, 266
TspRI CASTG 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 11, 314
XapI RAATTY 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 523
Zsp2I ATGCAT 2 cut(s) 98, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.