MD17G1178400.v1.1

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001575
Physical Location & Seq
Reverse (-)
20421895 .. 20422817
923 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1178400.v1.1.491

Sequence Viewer

Length: 573 bp
ATGCTTCCATTAAGCACCAAGGAAGGTGGGAAGAGGAAGACCACGGTAGTGGGACCATGGGGAGGAAATGGTGGAATTCCTTGGGACGATGGCATCTACAATGGCGTGAGAGAAATCACGGTTGTTTACGGCCACTGCATCGACTCCATCACCCTGGTGTATGATAAAAACGGCAAGCCTGTCAAAGCCAATACACATGGAGGCCGCGGAGGCACTCAGACCGCGGAGATAAAGTTGGAGTATCCGGATGAGTACCTAGTCAGTGTGACTGGGAACTACTGTCTGTTGGCTTACGGCGGCACAGCTATCATCCGTTCGCTTAAATTTCAGAGCAATCGAAGAACATTCGGACCTTTTGGGATAGAGGAAGGCACAACGTTTACTTACAATGTTGAAGGAGGAAAAATTGTTGGGTTGAAGGGAAGAAATGGTTGGTACATAGATGCCATTGGGTTTCATGTATCCCCTGCCCCTACAAAACTCTTTCGGAGAGTTCAGAAGAGTTTCAGAAGGCTCGGGAGCTCGGGCACCAGAGAAACTGAAACGACTGCTCCCAAGACTAAAGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBD Jacalin

Protein Analysis

191

Amino Acids

20.7

Weight (kDa)

9.67

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 17 - 153 2.8e-43 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 527
AccII CGCG 2 cut(s) 207, 224
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 5 cut(s) 205, 207, 222, 224, 297
AclI AACGTT 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 2 cut(s) 75, 323
AfaI GTAC 2 cut(s) 254, 437
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 2 cut(s) 395, 418
AjnI CCWGG 1 cut(s) 153
AjuI GAANNNNNNNTTGG 2 cut(s) 415, 447
AleI CACNNNNGTG 2 cut(s) 47, 155
AluBI AGCT 2 cut(s) 305, 522
AluI AGCT 2 cut(s) 305, 522
Alw21I GWGCWC 1 cut(s) 524
Ama87I CYCGRG 2 cut(s) 515, 523
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 2 cut(s) 130, 202
ApoI RAATTY 2 cut(s) 75, 323
Asp700I GAANNNNTTC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 53, 350
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 2 cut(s) 515, 523
AvaII GGWCC 2 cut(s) 53, 350
BaeGI GKGCMC 1 cut(s) 530
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 524
BccI CCATC 2 cut(s) 83, 155
BceAI ACGGC 3 cut(s) 145, 187, 310
BciT130I CCWGG 1 cut(s) 155
BciVI GTATCC 2 cut(s) 252, 472
BfaI CTAG 1 cut(s) 257
BfuI GTATCC 2 cut(s) 252, 472
BglI GCCNNNNNGGC 1 cut(s) 210
BisI GCNGC 2 cut(s) 205, 298
BlsI GCNGC 2 cut(s) 206, 299
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 2 cut(s) 53, 350
BmeT110I CYCGRG 2 cut(s) 515, 523
BmgT120I GGNCC 2 cut(s) 53, 350
BmiI GGNNCC 2 cut(s) 54, 529
BmrFI CCNGG 1 cut(s) 155
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 3 cut(s) 102, 147, 433
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 44
BsaJI CCNNGG 7 cut(s) 18, 42, 56, 80, 153, 205, 222
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 274
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 7 cut(s) 18, 42, 56, 80, 153, 205, 222
BseGI GGATG 2 cut(s) 253, 309
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 274
BseSI GKGCMC 1 cut(s) 530
Bsh1236I CGCG 2 cut(s) 207, 224
BshFI GGCC 2 cut(s) 132, 204
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 524
BsiHKCI CYCGRG 2 cut(s) 515, 523
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 2 cut(s) 66, 98
BslI CCNNNNNNNGG 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 66, 98
BsnI GGCC 2 cut(s) 132, 204
BsoBI CYCGRG 2 cut(s) 515, 523
Bsp1286I GDGCHC 2 cut(s) 524, 530
Bsp13I TCCGGA 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 5 cut(s) 205, 207, 222, 224, 297
BspANI GGCC 2 cut(s) 132, 204
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 207, 224
BspLI GGNNCC 2 cut(s) 54, 529
BspT107I GGYRCC 1 cut(s) 527
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 7 cut(s) 18, 42, 56, 80, 153, 205, 222
BssT1I CCWWGG 3 cut(s) 18, 56, 80
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 3 cut(s) 46, 121, 281
Bst6I CTCTTC 2 cut(s) 26, 494
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 4 cut(s) 42, 56, 205, 222
BstF5I GGATG 2 cut(s) 253, 309
BstFNI CGCG 2 cut(s) 207, 224
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 530
BstUI CGCG 2 cut(s) 207, 224
BstV2I GAAGAC 1 cut(s) 44
BstXI CCANNNNNNTGG 2 cut(s) 49, 154
BsuI GTATCC 2 cut(s) 252, 472
BsuRI GGCC 2 cut(s) 132, 204
BtgI CCRYGG 4 cut(s) 42, 56, 205, 222
BtsCI GGATG 2 cut(s) 253, 309
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 133, 268
Cac8I GCNNGC 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 53, 350
Cfr42I CCGCGG 2 cut(s) 208, 225
Csp6I GTAC 2 cut(s) 253, 436
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 3 cut(s) 57, 197, 458
CviJI RGCY 8 cut(s) 132, 178, 188, 204, 290, 305, 514, 522
CviKI_1 RGCY 8 cut(s) 132, 178, 188, 204, 290, 305, 514, 522
CviQI GTAC 2 cut(s) 253, 436
DdeI CTNAG 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 26, 494
EarI CTCTTC 2 cut(s) 26, 494
Ecl136II GAGCTC 1 cut(s) 522
Eco130I CCWWGG 3 cut(s) 18, 56, 80
Eco24I GRGCYC 1 cut(s) 524
Eco47I GGWCC 2 cut(s) 53, 350
Eco53kI GAGCTC 1 cut(s) 522
Eco88I CYCGRG 2 cut(s) 515, 523
EcoICRI GAGCTC 1 cut(s) 522
EcoRI GAATTC 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 3 cut(s) 18, 56, 80
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 3 cut(s) 18, 56, 80
FaeI CATG 3 cut(s) 60, 200, 461
FaiI YATR 5 cut(s) 58, 162, 198, 440, 459
FaqI GGGAC 2 cut(s) 66, 98
FatI CATG 3 cut(s) 56, 196, 457
Fnu4HI GCNGC 2 cut(s) 205, 298
FokI GGATG 2 cut(s) 260, 296
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 2 cut(s) 205, 298
FspBI CTAG 1 cut(s) 257
GluI GCNGC 2 cut(s) 205, 298
HaeIII GGCC 2 cut(s) 132, 204
HapII CCGG 1 cut(s) 245
Hin1II CATG 3 cut(s) 60, 200, 461
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 127, 381
Hpy188I TCNGA 6 cut(s) 219, 330, 350, 489, 498, 509
Hpy188III TCNNGA 2 cut(s) 245, 517
Hpy8I GTNNAC 2 cut(s) 127, 381
HpyAV CCTTC 5 cut(s) 17, 362, 389, 412, 504
HpyCH4III ACNGT 3 cut(s) 46, 121, 281
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 1 cut(s) 377
Hsp92II CATG 3 cut(s) 60, 200, 461
Kpn2I TCCGGA 1 cut(s) 244
KspI CCGCGG 2 cut(s) 208, 225
LmnI GCTCC 2 cut(s) 519, 556
LpnPI CCDG 7 cut(s) 140, 167, 192, 255, 258, 480, 544
LweI GCATC 3 cut(s) 102, 147, 433
MaeI CTAG 1 cut(s) 257
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 1 cut(s) 265
MboII GAAGA 5 cut(s) 43, 49, 351, 435, 511
MhlI GDGCHC 2 cut(s) 524, 530
MluCI AATT 3 cut(s) 75, 323, 405
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 6 cut(s) 27, 56, 194, 203, 358, 392
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 503
MseI TTAA 2 cut(s) 11, 321
MslI CAYNNNNRTG 2 cut(s) 47, 155
MspA1I CMGCKG 2 cut(s) 207, 224
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 2 cut(s) 207, 224
MwoI GCNNNNNNNGC 1 cut(s) 210
NcoI CCATGG 1 cut(s) 56
NlaIII CATG 3 cut(s) 60, 200, 461
NlaIV GGNNCC 2 cut(s) 54, 529
NmuCI GTSAC 1 cut(s) 265
OliI CACNNNNGTG 2 cut(s) 47, 155
PdmI GAANNNNTTC 1 cut(s) 503
PkrI GCNGC 2 cut(s) 206, 299
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 54, 529
PspPI GGNCC 2 cut(s) 53, 350
RsaI GTAC 2 cut(s) 254, 437
RsaNI GTAC 2 cut(s) 253, 436
RseI CAYNNNNRTG 2 cut(s) 47, 155
SacI GAGCTC 1 cut(s) 524
SacII CCGCGG 2 cut(s) 208, 225
SaqAI TTAA 2 cut(s) 11, 321
SatI GCNGC 2 cut(s) 205, 298
Sau96I GGNCC 2 cut(s) 53, 350
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 2 cut(s) 524, 530
SetI ASST 6 cut(s) 28, 258, 307, 355, 380, 524
SfaNI GCATC 3 cut(s) 102, 147, 433
Sfr303I CCGCGG 2 cut(s) 208, 225
SgrBI CCGCGG 2 cut(s) 208, 225
SinI GGWCC 2 cut(s) 53, 350
SmiMI CAYNNNNRTG 2 cut(s) 47, 155
Sse9I AATT 3 cut(s) 75, 323, 405
SsiI CCGC 5 cut(s) 205, 207, 222, 224, 297
SspMI CTAG 1 cut(s) 257
SstI GAGCTC 1 cut(s) 524
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 3 cut(s) 18, 56, 80
TaaI ACNGT 3 cut(s) 46, 121, 281
TaiI ACGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 141, 337
TasI AATT 3 cut(s) 75, 323, 405
TauI GCSGC 2 cut(s) 207, 300
Tru1I TTAA 2 cut(s) 11, 321
Tru9I TTAA 2 cut(s) 11, 321
TscAI CASTG 2 cut(s) 140, 268
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 446
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 2 cut(s) 140, 268
VpaK11BI GGWCC 2 cut(s) 53, 350
XapI RAATTY 2 cut(s) 75, 323
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.