MD10G1311000.v1.1

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002039
Physical Location & Seq
Reverse (-)
39589947 .. 39593656
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1311000.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGGAATTCAGAGTTCACTTGCAAGCTGATAATGAGCAGAAATTTTTTCAAAACCAGATGAAACCCGAACCTGAAGCCTCTTACTTGATTAATCAAAGACGGTCTGCAAATTACAAGCCAAATATTTGGAAGAACGATTTCCTAGATCAATCTCTTATCAGCAAATACGATGGAGATGAGTATCGGAAGCTGTCTGAGAAGTTAATAGAAGAAGTTAAGATTTATATATCTGCTGAAACAATGGATTTAGTAGCTAAGTTGGAGCTCATTGACAGCGTCCGAAAACTAGGCCTCGCGAACCTCTTCGAAAAGGAAATCAAGGAAGCCCTAGACAGCATTGCAGCTATCGAAAGCGACAATCTCGGCACAAGAGACGATCTCTATGGTACTGCATTACACTTCAAGATCCTCAGGCAGCATGGCTATAAAGTTTCACAAGATATATTTGGTAGATTCATGGATGAAAAGGGCACATTAGAGAACCACCATTTCGCGCATTTAAAAGGAATGCTGGAACTTTTCGAGGCCTCAAACCTGGGTTTCGAAGGTGAAGATATTTTAGATGAGGCGAAAGCTTCCTTGACGCTAGCTCTCAGAGATAGTGGTCATATTTGTTATCCAGACAGTAACCTTTCCAGGGACGTAGTTCATTCCCTGGAGCTTCCATCACACCGCAGAGTGCAGTGGTTTGATGTCAAATGGCAAATCAACGCCTATGAAAAAGACATTTGTCGCGTCAACGCCACGTTACTCGAATTAGCAAAGCTTAATTTCAACGTAGTTCAGGCCCAACTCCAAAAAAACTTAAGGGAAGCATCCAGGTGGTGGGCAAATCTGGGCTTCGCAGACAACTTGAAATTTGCAAGAGATAGACTGGTTGAATGTTTCTCATGTGCTGTGGGAGTAGCATTCGAGCCTGAGCACTCATCTTTTAGAATATGTCTTACCAAAGTCATCAACTTAGTACTGATCATAGACGACGTCTATGATATTTATGGCTCAGAGGAAGAGCTAAAGCACTTCACCAATGCTGTTGATAGGTGGGATTCTAGGGAAACTGAGCAGCTTCCAGAGTGTATGAAGATGTGTTTCCAAGTACTCTACAACACTACTTGTGAAATTGCTCGTGAAATTGAGGAGGAGAATGGTTGGAACCAAGTATTACCTCAATTGACCAAAGTGTGGGCAGATTTTTGTAAAGCATTATTGGTGGAGGCAGAGTGGTATAATAAGAGCCATATACCAACCCTTGAAGAGTACCTAAGAAACGGATGCATTTCATCATCAGTTTCAGTGCTTTTGGTTCACTCGTTTTTCTCTATAACTCATGAGGGAACCAAAGAGATGGCTGATTTTCTTCACAAGAATGAAGATCTTTTGTATAATATCTCTCTCATCGTTCGCCTCAACAATGATTTGGGAACTTCCGTGGCTGAACAAGAGAGAGGGGATTCTCCTTCATCAATCGTATGTTACATGAGAGAAGTGAATGCCTCTGAAGAAACAGCTAGGAAGAACATTAAGGGCATGATAGACAATGCATGGAAGAAAGTAAATGGAAAATGCTTCACAACAAACCAAGTGCCTTTTCTGTCATCATTCATGAACAATGCCACAAACATGGCACGTGTGGCGCACAGCCTTTACAAAGATGGAGATGGGTTTGGTGACCAAGAGAAAGGGCCTCGGACCCACATCCTGTCTTTACTATTCCAACCTCTTGTAAACTAG

Protein Analysis

577

Amino Acids

66.29

Weight (kDa)

5.27

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 42 - 219 2.6e-40 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 278 - 517 6.3e-88 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 317 - 517 2.1e-54 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 984
AccB7I CCANNNNNTGG 2 cut(s) 825, 1182
AccII CGCG 3 cut(s) 296, 494, 735
AciI CCGC 1 cut(s) 673
AclWI GGATC 1 cut(s) 400
AcsI RAATTY 3 cut(s) 5, 41, 857
AcuI CTGAAG 2 cut(s) 93, 1518
AcvI CACGTG 1 cut(s) 1628
AcyI GRCGYC 1 cut(s) 981
AfaI GTAC 4 cut(s) 388, 966, 1098, 1259
AfiI CCNNNNNNNGG 3 cut(s) 637, 825, 1182
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 1627
AgsI TTSAA 6 cut(s) 50, 403, 775, 856, 881, 1253
AjnI CCWGG 4 cut(s) 534, 635, 654, 818
AjuI GAANNNNNNNTTGG 2 cut(s) 1572, 1604
Alw21I GWGCWC 2 cut(s) 267, 924
Alw26I GTCTC 1 cut(s) 366
AlwI GGATC 1 cut(s) 400
AoxI GGCC 4 cut(s) 289, 525, 786, 1682
ApeKI GCWGC 3 cut(s) 341, 415, 1063
ApoI RAATTY 3 cut(s) 5, 41, 857
AseI ATTAAT 1 cut(s) 90
Asp700I GAANNNNTTC 2 cut(s) 137, 302
AspLEI GCGC 2 cut(s) 496, 1636
AspS9I GGNCC 3 cut(s) 787, 1682, 1689
AsuHPI GGTGA 3 cut(s) 560, 1015, 1679
AsuII TTCGAA 2 cut(s) 306, 543
AsuNHI GCTAGC 1 cut(s) 586
AvaII GGWCC 1 cut(s) 1689
AxyI CCTNAGG 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 1125
BbrPI CACGTG 1 cut(s) 1628
Bbv12I GWGCWC 2 cut(s) 267, 924
BbvI GCAGC 3 cut(s) 353, 427, 1075
BccI CCATC 5 cut(s) 164, 673, 1339, 1646, 1652
BciT130I CCWGG 4 cut(s) 536, 637, 656, 820
BclI TGATCA 1 cut(s) 969
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 7 cut(s) 143, 287, 329, 587, 1050, 1509, 1729
BfrI CTTAAG 1 cut(s) 805
BglII AGATCT 1 cut(s) 1372
BisI GCNGC 3 cut(s) 342, 416, 1064
BlsI GCNGC 3 cut(s) 343, 417, 1065
BmcAI AGTACT 2 cut(s) 966, 1098
Bme1390I CCNGG 4 cut(s) 536, 637, 656, 820
Bme18I GGWCC 1 cut(s) 1689
BmgT120I GGNCC 3 cut(s) 787, 1682, 1689
BmiI GGNNCC 3 cut(s) 1154, 1336, 1691
BmrFI CCNGG 4 cut(s) 536, 637, 656, 820
BmsI GCATC 2 cut(s) 824, 1262
BmtI GCTAGC 1 cut(s) 590
BoxI GACNNNNGTC 1 cut(s) 729
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BpmI CTGGAG 1 cut(s) 677
Bpu10I CCTNAGC 1 cut(s) 918
Bpu14I TTCGAA 2 cut(s) 306, 543
BsaAI YACGTR 1 cut(s) 1628
BsaBI GATNNNNATC 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 981
BsaJI CCNNGG 5 cut(s) 535, 636, 654, 1428, 1685
BsaXI ACNNNNNCTCC 2 cut(s) 1205, 1235
Bsc4I CCNNNNNNNGG 3 cut(s) 637, 825, 1182
Bse1I ACTGG 1 cut(s) 879
Bse21I CCTNAGG 1 cut(s) 410
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 4 cut(s) 536, 637, 656, 820
BseDI CCNNGG 5 cut(s) 535, 636, 654, 1428, 1685
BseGI GGATG 4 cut(s) 466, 815, 1277, 1695
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 3 cut(s) 637, 825, 1182
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 6 cut(s) 186, 424, 607, 909, 1014, 1050
BseNI ACTGG 1 cut(s) 879
BseRI GAGGAG 2 cut(s) 1151, 1154
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 3 cut(s) 353, 427, 1075
BsgI GTGCAG 1 cut(s) 701
Bsh1236I CGCG 3 cut(s) 296, 494, 735
BshFI GGCC 4 cut(s) 291, 527, 788, 1684
BsiHKAI GWGCWC 2 cut(s) 267, 924
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 637, 825, 1182
BsmAI GTCTC 1 cut(s) 366
BsmBI CGTCTC 1 cut(s) 366
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 3 cut(s) 513, 908, 1495
BsnI GGCC 4 cut(s) 291, 527, 788, 1684
Bsp119I TTCGAA 2 cut(s) 306, 543
Bsp1286I GDGCHC 3 cut(s) 267, 473, 924
Bsp143I GATC 5 cut(s) 145, 376, 405, 969, 1372
Bsp68I TCGCGA 1 cut(s) 296
BspACI CCGC 1 cut(s) 673
BspANI GGCC 4 cut(s) 291, 527, 788, 1684
BspCNI CTCAG 6 cut(s) 187, 423, 606, 910, 1013, 1051
BspFNI CGCG 3 cut(s) 296, 494, 735
BspHI TCATGA 2 cut(s) 1327, 1602
BspLI GGNNCC 3 cut(s) 1154, 1336, 1691
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 1 cut(s) 400
BspQI GCTCTTC 1 cut(s) 1002
BspT104I TTCGAA 2 cut(s) 306, 543
BspTI CTTAAG 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 1 cut(s) 879
BssECI CCNNGG 5 cut(s) 535, 636, 654, 1428, 1685
BssMI GATC 5 cut(s) 145, 376, 405, 969, 1372
BssNI GRCGYC 1 cut(s) 981
BssSI CACGAG 1 cut(s) 1125
Bst2BI CACGAG 1 cut(s) 1125
Bst2UI CCWGG 4 cut(s) 536, 637, 656, 820
Bst4CI ACNGT 2 cut(s) 102, 626
Bst6I CTCTTC 3 cut(s) 308, 1002, 1248
BstACI GRCGYC 1 cut(s) 981
BstAFI CTTAAG 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 1628
BstBI TTCGAA 2 cut(s) 306, 543
BstC8I GCNNGC 2 cut(s) 24, 588
BstDEI CTNAG 9 cut(s) 195, 255, 410, 593, 918, 961, 1000, 1059, 1262
BstDSI CCRYGG 1 cut(s) 1428
BstEII GGTNACC 1 cut(s) 1667
BstF5I GGATG 4 cut(s) 466, 815, 1277, 1695
BstFNI CGCG 3 cut(s) 296, 494, 735
BstHHI GCGC 2 cut(s) 496, 1636
BstKTI GATC 5 cut(s) 148, 379, 408, 972, 1375
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 5 cut(s) 145, 376, 405, 969, 1372
BstMWI GCNNNNNNNGC 1 cut(s) 1631
BstNI CCWGG 4 cut(s) 536, 637, 656, 820
BstPAI GACNNNNGTC 1 cut(s) 729
BstPI GGTNACC 1 cut(s) 1667
BstSCI CCNGG 4 cut(s) 534, 635, 654, 818
BstSLI GKGCMC 1 cut(s) 473
BstUI CGCG 3 cut(s) 296, 494, 735
BstV1I GCAGC 3 cut(s) 353, 427, 1075
BstX2I RGATCY 2 cut(s) 405, 1372
BstXI CCANNNNNNTGG 3 cut(s) 126, 1345, 1621
BstYI RGATCY 2 cut(s) 405, 1372
Bsu36I CCTNAGG 1 cut(s) 410
BsuRI GGCC 4 cut(s) 291, 527, 788, 1684
BtgI CCRYGG 1 cut(s) 1428
BtsCI GGATG 4 cut(s) 466, 815, 1277, 1695
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 2 cut(s) 689, 1299
BtuMI TCGCGA 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 24, 588
CciI TCATGA 2 cut(s) 1327, 1602
CfoI GCGC 2 cut(s) 496, 1636
Cfr13I GGNCC 3 cut(s) 787, 1682, 1689
CseI GACGC 3 cut(s) 265, 592, 724
Csp6I GTAC 4 cut(s) 387, 965, 1097, 1258
CviAII CATG 9 cut(s) 419, 457, 891, 1328, 1477, 1528, 1542, 1603, 1621
CviQI GTAC 4 cut(s) 387, 965, 1097, 1258
DdeI CTNAG 9 cut(s) 195, 255, 410, 593, 918, 961, 1000, 1059, 1262
DpnI GATC 5 cut(s) 147, 378, 407, 971, 1374
DpnII GATC 5 cut(s) 145, 376, 405, 969, 1372
DraI TTTAAA 1 cut(s) 501
Eam1104I CTCTTC 3 cut(s) 308, 1002, 1248
EarI CTCTTC 3 cut(s) 308, 1002, 1248
Ecl136II GAGCTC 1 cut(s) 265
Eco147I AGGCCT 2 cut(s) 291, 527
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 1689
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 93, 1518
Eco72I CACGTG 1 cut(s) 1628
Eco81I CCTNAGG 1 cut(s) 410
Eco91I GGTNACC 1 cut(s) 1667
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 1682
EcoO65I GGTNACC 1 cut(s) 1667
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 4 cut(s) 534, 635, 654, 818
EcoT22I ATGCAT 2 cut(s) 1277, 1543
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 366
FaeI CATG 9 cut(s) 422, 460, 894, 1331, 1480, 1531, 1545, 1606, 1624
FaqI GGGAC 1 cut(s) 653
FatI CATG 9 cut(s) 418, 456, 890, 1327, 1476, 1527, 1541, 1602, 1620
FbaI TGATCA 1 cut(s) 969
Fnu4HI GCNGC 3 cut(s) 342, 416, 1064
FokI GGATG 4 cut(s) 473, 802, 1284, 1682
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 342, 416, 1064
FspBI CTAG 7 cut(s) 143, 287, 329, 587, 1050, 1509, 1729
GlaI GCGC 2 cut(s) 495, 1635
GluI GCNGC 3 cut(s) 342, 416, 1064
GsuI CTGGAG 1 cut(s) 677
HaeIII GGCC 4 cut(s) 291, 527, 788, 1684
HgaI GACGC 3 cut(s) 265, 592, 724
HhaI GCGC 2 cut(s) 496, 1636
Hin1I GRCGYC 1 cut(s) 981
Hin1II CATG 9 cut(s) 422, 460, 894, 1331, 1480, 1531, 1545, 1606, 1624
Hin6I GCGC 2 cut(s) 494, 1634
HinP1I GCGC 2 cut(s) 494, 1634
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 573, 764
HinfI GANTC 3 cut(s) 453, 1046, 1451
HphI GGTGA 3 cut(s) 560, 1015, 1679
Hpy166II GTNNAC 4 cut(s) 16, 739, 1306, 1726
Hpy188I TCNGA 8 cut(s) 11, 186, 196, 281, 596, 1003, 1498, 1689
Hpy188III TCNNGA 7 cut(s) 295, 403, 620, 1070, 1127, 1328, 1603
Hpy8I GTNNAC 4 cut(s) 16, 739, 1306, 1726
Hpy99I CGWCG 1 cut(s) 983
HpyAV CCTTC 2 cut(s) 539, 1467
HpyCH4III ACNGT 2 cut(s) 102, 626
HpyCH4IV ACGT 5 cut(s) 642, 746, 777, 981, 1627
HpyCH4V TGCA 8 cut(s) 22, 107, 341, 392, 682, 863, 1275, 1541
HpyF10VI GCNNNNNNNGC 1 cut(s) 1631
HpyF3I CTNAG 9 cut(s) 195, 255, 410, 593, 918, 961, 1000, 1059, 1262
HpySE526I ACGT 5 cut(s) 642, 746, 777, 981, 1627
Hsp92I GRCGYC 1 cut(s) 981
Hsp92II CATG 9 cut(s) 422, 460, 894, 1331, 1480, 1531, 1545, 1606, 1624
HspAI GCGC 2 cut(s) 494, 1634
Ksp22I TGATCA 1 cut(s) 969
Kzo9I GATC 5 cut(s) 145, 376, 405, 969, 1372
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 2 cut(s) 262, 658
Lsp1109I GCAGC 3 cut(s) 353, 427, 1075
LweI GCATC 2 cut(s) 824, 1262
MaeI CTAG 7 cut(s) 143, 287, 329, 587, 1050, 1509, 1729
MaeII ACGT 5 cut(s) 642, 746, 777, 981, 1627
MaeIII GTNAC 4 cut(s) 626, 747, 1472, 1667
MalI GATC 5 cut(s) 147, 378, 407, 971, 1374
MboI GATC 5 cut(s) 145, 376, 405, 969, 1372
MfeI CAATTG 1 cut(s) 1169
MflI RGATCY 2 cut(s) 405, 1372
MhlI GDGCHC 3 cut(s) 267, 473, 924
MluCI AATT 9 cut(s) 5, 41, 109, 755, 769, 857, 1119, 1131, 1169
MmeI TCCRAC 2 cut(s) 240, 1130
Mph1103I ATGCAT 2 cut(s) 1277, 1543
MroXI GAANNNNTTC 2 cut(s) 137, 302
MseI TTAA 7 cut(s) 90, 203, 216, 500, 768, 806, 1521
MslI CAYNNNNRTG 3 cut(s) 820, 1365, 1619
MspCI CTTAAG 1 cut(s) 805
MspR9I CCNGG 4 cut(s) 536, 637, 656, 820
MunI CAATTG 1 cut(s) 1169
Mva1269I GAATGC 3 cut(s) 513, 908, 1495
MvaI CCWGG 4 cut(s) 536, 637, 656, 820
MvnI CGCG 3 cut(s) 296, 494, 735
MwoI GCNNNNNNNGC 1 cut(s) 1631
NdeII GATC 5 cut(s) 145, 376, 405, 969, 1372
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 9 cut(s) 422, 460, 894, 1331, 1480, 1531, 1545, 1606, 1624
NlaIV GGNNCC 3 cut(s) 1154, 1336, 1691
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 1667
NruI TCGCGA 1 cut(s) 296
NsiI ATGCAT 2 cut(s) 1277, 1543
NspV TTCGAA 2 cut(s) 306, 543
PagI TCATGA 2 cut(s) 1327, 1602
PceI AGGCCT 2 cut(s) 291, 527
PciSI GCTCTTC 1 cut(s) 1002
PctI GAATGC 3 cut(s) 513, 908, 1495
PdmI GAANNNNTTC 2 cut(s) 137, 302
PfeI GAWTC 3 cut(s) 453, 1046, 1451
PflFI GACNNNGTC 2 cut(s) 275, 980
PflMI CCANNNNNTGG 2 cut(s) 825, 1182
PkrI GCNGC 3 cut(s) 343, 417, 1065
PmaCI CACGTG 1 cut(s) 1628
PmlI CACGTG 1 cut(s) 1628
Ppu21I YACGTR 1 cut(s) 1628
PshAI GACNNNNGTC 1 cut(s) 729
PshBI ATTAAT 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 4 cut(s) 534, 635, 654, 818
PspCI CACGTG 1 cut(s) 1628
PspEI GGTNACC 1 cut(s) 1667
PspGI CCWGG 4 cut(s) 534, 635, 654, 818
PspN4I GGNNCC 3 cut(s) 1154, 1336, 1691
PspPI GGNCC 3 cut(s) 787, 1682, 1689
PsuI RGATCY 2 cut(s) 405, 1372
PsyI GACNNNGTC 2 cut(s) 275, 980
RruI TCGCGA 1 cut(s) 296
RsaI GTAC 4 cut(s) 388, 966, 1098, 1259
RsaNI GTAC 4 cut(s) 387, 965, 1097, 1258
RseI CAYNNNNRTG 3 cut(s) 820, 1365, 1619
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 7 cut(s) 90, 203, 216, 500, 768, 806, 1521
SatI GCNGC 3 cut(s) 342, 416, 1064
Sau3AI GATC 5 cut(s) 145, 376, 405, 969, 1372
Sau96I GGNCC 3 cut(s) 787, 1682, 1689
ScaI AGTACT 2 cut(s) 966, 1098
ScrFI CCNGG 4 cut(s) 536, 637, 656, 820
SduI GDGCHC 3 cut(s) 267, 473, 924
SfaNI GCATC 2 cut(s) 824, 1262
SfuI TTCGAA 2 cut(s) 306, 543
SinI GGWCC 1 cut(s) 1689
SmiMI CAYNNNNRTG 3 cut(s) 820, 1365, 1619
SmlI CTYRAG 1 cut(s) 805
SmoI CTYRAG 1 cut(s) 805
Sse9I AATT 9 cut(s) 5, 41, 109, 755, 769, 857, 1119, 1131, 1169
SseBI AGGCCT 2 cut(s) 291, 527
SsiI CCGC 1 cut(s) 673
SspI AATATT 1 cut(s) 124
SspMI CTAG 7 cut(s) 143, 287, 329, 587, 1050, 1509, 1729
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 2 cut(s) 291, 527
StyD4I CCNGG 4 cut(s) 534, 635, 654, 818
TaaI ACNGT 2 cut(s) 102, 626
TaiI ACGT 5 cut(s) 645, 749, 780, 984, 1630
TaqI TCGA 6 cut(s) 306, 348, 522, 543, 753, 912
TasI AATT 9 cut(s) 5, 41, 109, 755, 769, 857, 1119, 1131, 1169
TatI WGTACW 2 cut(s) 964, 1096
TfiI GAWTC 3 cut(s) 453, 1046, 1451
Tru1I TTAA 7 cut(s) 90, 203, 216, 500, 768, 806, 1521
Tru9I TTAA 7 cut(s) 90, 203, 216, 500, 768, 806, 1521
TscAI CASTG 2 cut(s) 689, 1299
TseFI GTSAC 1 cut(s) 1667
TseI GCWGC 3 cut(s) 341, 415, 1063
Tsp45I GTSAC 1 cut(s) 1667
TspGWI ACGGA 2 cut(s) 1284, 1417
TspRI CASTG 2 cut(s) 689, 1299
Tth111I GACNNNGTC 2 cut(s) 275, 980
Van91I CCANNNNNTGG 2 cut(s) 825, 1182
Vha464I CTTAAG 1 cut(s) 805
VpaK11BI GGWCC 1 cut(s) 1689
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 3 cut(s) 5, 41, 857
XmnI GAANNNNTTC 2 cut(s) 137, 302
XspI CTAG 7 cut(s) 143, 287, 329, 587, 1050, 1509, 1729
ZraI GACGTC 1 cut(s) 982
ZrmI AGTACT 2 cut(s) 966, 1098
Zsp2I ATGCAT 2 cut(s) 1277, 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.