RchiOBHm_Chr5g0029171

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002039
Physical Location & Seq
Forward (+)
22892388 .. 22893425
1038 bp
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UTR
Exon/CDS
Intron
PRQ30858

Sequence Viewer

Length: 666 bp
ATGTGGCTCCGCCTCTGGATACTGCAATGGCAGACGAAATCCGAACCCCCTTCCTCGGTACATGATCAAAGACGATCTGCTAATTACAAGCCGAATATTTGGAAGTATGATTTCCTTGAATCTCTTAATAGCAAATTTGATGGAGAATGTTATCGAATTCAGATTCAGAAGCTAATAGAAGATGTTAAGGATTTATTTGGTGAATGTAAGGAATTAGGTTTACTAGCTAAGTTGGAGCTGATTGACAGCATCCAAAAACTCGGCCTGAACAGCTACTTTGAGACGGAAATCAAGGAAACCCTAGACACCATAGCATCTGTCGAACTGAAAAACAATAGCAATCCCTTCATTAGTTCAGAGGGTGATCTCTACGGTACGGCACTACTCTTTAAGATTCTGAGATGTATTTGGTGTTTTCATAGACGAAATTGGTACATCAAAGAAGAGCACATTTGGGATGTGAAAGGAATGCTAGAACTTTTGGAGGCCTCAAACTTAGCTTTAGAAGGTGAAAACATCCTAGATGAGGCTAAAGCTTTCTTAATGGTCACTCTCAGAGATACCAATACCATGTGTGATGATATAGACCGTTGCATTTCCAAGCATGTGGCCTATGCCTTGAAGCTTTCATCACAAAGGAGAGTCCAGTGGTTTAATGTGAAATGA

Protein Analysis

221

Amino Acids

26.02

Weight (kDa)

5.68

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 33 - 207 3.1e-27 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 10
AcsI RAATTY 2 cut(s) 134, 156
AfaI GTAC 3 cut(s) 60, 376, 434
AfiI CCNNNNNNNGG 2 cut(s) 55, 526
AgsI TTSAA 2 cut(s) 119, 622
AluBI AGCT 7 cut(s) 172, 227, 238, 273, 500, 536, 625
AluI AGCT 7 cut(s) 172, 227, 238, 273, 500, 536, 625
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 275
AoxI GGCC 3 cut(s) 262, 486, 609
ApoI RAATTY 2 cut(s) 134, 156
AsuHPI GGTGA 3 cut(s) 212, 374, 521
Bbv12I GWGCWC 1 cut(s) 450
BccI CCATC 1 cut(s) 134
BceAI ACGGC 1 cut(s) 393
BciVI GTATCC 1 cut(s) 12
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 4 cut(s) 224, 302, 473, 521
BfuI GTATCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 8
BmsI GCATC 2 cut(s) 258, 323
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 526
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 3 cut(s) 249, 463, 516
BseLI CCNNNNNNNGG 2 cut(s) 55, 526
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 389, 568
BseNI ACTGG 1 cut(s) 646
BshFI GGCC 3 cut(s) 264, 488, 611
BsiHKAI GWGCWC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 55, 526
BsmAI GTCTC 1 cut(s) 275
BsmBI CGTCTC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 3 cut(s) 264, 488, 611
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 3 cut(s) 64, 74, 364
BspACI CCGC 1 cut(s) 10
BspANI GGCC 3 cut(s) 264, 488, 611
BspCNI CTCAG 2 cut(s) 390, 567
BspLI GGNNCC 1 cut(s) 8
BspQI GCTCTTC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 32
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 3 cut(s) 64, 74, 364
Bst4CI ACNGT 2 cut(s) 374, 590
Bst6I CTCTTC 1 cut(s) 438
BstDEI CTNAG 4 cut(s) 228, 398, 496, 554
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 3 cut(s) 249, 463, 516
BstKTI GATC 3 cut(s) 67, 77, 367
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 3 cut(s) 64, 74, 364
BstMWI GCNNNNNNNGC 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 608
BstXI CCANNNNNNTGG 1 cut(s) 607
BsuI GTATCC 1 cut(s) 12
BsuRI GGCC 3 cut(s) 264, 488, 611
BtsCI GGATG 3 cut(s) 249, 463, 516
BtsIMutI CAGTG 1 cut(s) 653
Csp6I GTAC 3 cut(s) 59, 375, 433
CviAII CATG 3 cut(s) 62, 571, 605
CviQI GTAC 3 cut(s) 59, 375, 433
DdeI CTNAG 4 cut(s) 228, 398, 496, 554
DpnI GATC 3 cut(s) 66, 76, 366
DpnII GATC 3 cut(s) 64, 74, 364
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 488
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 3 cut(s) 65, 574, 608
FaiI YATR 8 cut(s) 63, 108, 311, 420, 572, 584, 606, 615
FatI CATG 3 cut(s) 61, 570, 604
FbaI TGATCA 1 cut(s) 64
FokI GGATG 3 cut(s) 236, 470, 503
FspBI CTAG 4 cut(s) 224, 302, 473, 521
HaeIII GGCC 3 cut(s) 264, 488, 611
Hin1II CATG 3 cut(s) 65, 574, 608
HindIII AAGCTT 2 cut(s) 534, 623
HinfI GANTC 4 cut(s) 119, 163, 394, 642
HphI GGTGA 3 cut(s) 212, 374, 521
Hpy166II GTNNAC 1 cut(s) 221
Hpy188I TCNGA 6 cut(s) 43, 162, 168, 358, 399, 557
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 60, 355, 500
HpyCH4III ACNGT 2 cut(s) 374, 590
HpyCH4V TGCA 2 cut(s) 25, 594
HpyF10VI GCNNNNNNNGC 1 cut(s) 270
HpyF3I CTNAG 4 cut(s) 228, 398, 496, 554
Hsp92II CATG 3 cut(s) 65, 574, 608
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 3 cut(s) 64, 74, 364
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 12, 235
LpnPI CCDG 2 cut(s) 278, 659
LweI GCATC 2 cut(s) 258, 323
MaeI CTAG 4 cut(s) 224, 302, 473, 521
MaeIII GTNAC 1 cut(s) 547
MalI GATC 3 cut(s) 66, 76, 366
MboI GATC 3 cut(s) 64, 74, 364
MboII GAAGA 2 cut(s) 191, 455
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 5 cut(s) 82, 134, 156, 212, 427
MlyI GAGTC 1 cut(s) 651
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 6 cut(s) 23, 64, 352, 478, 499, 520
MseI TTAA 5 cut(s) 126, 186, 390, 542, 654
Mva1269I GAATGC 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 270
NdeII GATC 3 cut(s) 64, 74, 364
NlaIII CATG 3 cut(s) 65, 574, 608
NlaIV GGNNCC 1 cut(s) 8
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 1 cut(s) 608
PceI AGGCCT 1 cut(s) 488
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 1 cut(s) 474
PfeI GAWTC 3 cut(s) 119, 163, 394
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
PspN4I GGNNCC 1 cut(s) 8
RsaI GTAC 3 cut(s) 60, 376, 434
RsaNI GTAC 3 cut(s) 59, 375, 433
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 5 cut(s) 126, 186, 390, 542, 654
Sau3AI GATC 3 cut(s) 64, 74, 364
SchI GAGTC 1 cut(s) 651
SduI GDGCHC 1 cut(s) 450
SetI ASST 9 cut(s) 174, 220, 229, 240, 275, 502, 511, 538, 627
SfaNI GCATC 2 cut(s) 258, 323
Sse9I AATT 5 cut(s) 82, 134, 156, 212, 427
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 1 cut(s) 10
SspI AATATT 1 cut(s) 97
SspMI CTAG 4 cut(s) 224, 302, 473, 521
StuI AGGCCT 1 cut(s) 488
TaaI ACNGT 2 cut(s) 374, 590
TaqI TCGA 2 cut(s) 154, 321
TasI AATT 5 cut(s) 82, 134, 156, 212, 427
TfiI GAWTC 3 cut(s) 119, 163, 394
Tru1I TTAA 5 cut(s) 126, 186, 390, 542, 654
Tru9I TTAA 5 cut(s) 126, 186, 390, 542, 654
TscAI CASTG 1 cut(s) 653
TseFI GTSAC 1 cut(s) 547
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 337, 407, 618
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 653
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 2 cut(s) 134, 156
XceI RCATGY 1 cut(s) 608
XspI CTAG 4 cut(s) 224, 302, 473, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.