Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002039

Physical Location & Seq

Genomic Coordinates Forward (+)

20542876 .. 20545390

Gene Structure 2515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G009340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGGATTCTAGTATGCACTTACAAGACCCTCAAGTTGATGAGCAACAGATACTGCAATGGCAGACGAAATCTGAACCCTCTTACTTTGTACATGATCAAAGACGATTTGCAAATTACAAGCCAAATATTTGGAAGTATGAGTTCCTCGAATCCCTTAATAGCAAATTTGATGGAGAATGTTATCGAATTCAGATTCAGAAGCTAATAGAAGATGTAAAGGAAATGTTTGCTGATTGTAAGGAATCAGATTTAATAACCAAGTTGGAGTTGCTTGACAGCATCCAAAAACTAGGCCTGAACAACCATTTTGGAAAGGAAATCAAGAAAGCCCTAGACACCATAACATCTGTTGAACTCGAAAATAACAGCAATCCATACATTAGTTCAAGAGGTGATCTCTATGCTGCTGCACTACTCTTTAAGATTCTTAGGCAGCATGGTTACAAAGTTTCACAAGATATATTTGGTGGTTTGATGGATGAAATGGGTACATTAAAGAAGAAAACATTTGGGGATGTGAAAGGAATGCTTGAAATGTTGGAGGCCTCAAACCTAGCTTTAGAAGGTGAATATATCCTAGAGGAGGCTAAAGCTTTCTTAATGGTCACTCTCAGAGATACCAATACTAGGTGTGATGATATAGACAATTGCATTTTCAAGCATGTGATTTATACACTGGAGCTTCCATCACAAAGGAGAGTGCAGTGGTTTAATGTGAAATGGCACATCAAATCCTACGAGAAAGACCATACTAATCAAGCCAACATGACCACTTTACTTGAGTTGGCTAAACTTAACTTCAACATGGTTCAAGCCATACTCCAAAAAGATCTAAGGGAAGTATCCATGTGGTGGAACAATCTAGGTCTCAATAAGAACTTGGACTTTGCAAGAGATAGACTGGTTGAATGTTTCATGTGTGCCGTGGGATTAGCTTTCGAGCCTGAGTACAAGTCCTTTAGAAAATGGATAACTAAAGTCATCAATTTAATTCTCATCATCGATGACGTTTATGATGTTTATGGCTCATTAGAAGAGCTAAAACATTTCACCAATGCTGTTAAAAGGTGGGATATTAAGGAAACCGAGCAGCTTCCGGAGTGTATGAAGATCTGTTTCCAAGTACTCTACAATACTACTTGTGAAATTGCTTATGAAATTGAGGAGGAGAAAGGTTGGAACCAAGTATTACCTCATTTGCGGAAAGTGTGGGGAGATTTCTGTAAAGCATTATTGGTGGAGGCAGAGTGGTACAATAAGGCCTACACACCGTCCTTTGAAGAACACCTTAGCAATGGTTGCATTTCATCATCTACTTTGCTGGCTTTGACTCATGCCTTCTTCTCCATGACACGTGCTGAAGGGGCCAAAAACATTGCTGATTTTCTGCACAAGAATGAAGATCTTGTGTACAATATCTCTTTGATTGTTCGACTCTTAAACGATCTGGGAACTTCTGTGGCTGAGCAAAAGAGAGGTGATGTTGCTTCATCAATCCTATGTTACATGAGAGAAGCGAATGCTTCAGAAGAAATAGCTCGAAATCACATCAAAGGCATGATAGACAATGCATGGAAGAAAATAAACGAAAAATGCTTCACTCAAATGCCTGAAATATCTTCATTCTTCAACATTATGACAAATATAGCTCGGATTGGGCATAGCCTTTATCAAGATGGAGATGGATTTGGTGATCAAGAGCACGGGACTCGGGCACAGATCCAATCTTTACTAGTTGAACCTCTCTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0010333 GO:0010334 GO:0016829 GO:0016835 GO:0016838 GO:0042214 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046246 GO:0051761 GO:0051762 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

583

Amino Acids

67.6

Weight (kDa)

5.37

Isoelectric Point (pI)

41.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	43 - 225	3.4e-40	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	287 - 527	3.9e-89	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	326 - 527	2.8e-54	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002039)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g03000
malus_domestica	MD10G1311000.v1.1
prunus_persica	Prupe.4G029900_v2.0.a1 Prupe.4G125200_v2.0.a1 Prupe.4G125300_v2.0.a1 Prupe.4G125300_v2.0.a1 Prupe.4G125500_v2.0.a1 Prupe.4G126400_v2.0.a1 Prupe.4G126600_v2.0.a1 Prupe.4G126600_v2.0.a1
pyrus_communis	pycom10g26190
rosa_chinensis	RchiOBHm_Chr1g0331201 RchiOBHm_Chr1g0331211 RchiOBHm_Chr5g0004591 RchiOBHm_Chr5g0004631 RchiOBHm_Chr5g0029171 RchiOBHm_Chr5g0029181 RchiOBHm_Chr5g0029221 RchiOBHm_Chr5g0029231
rosa_laevigata	RLG00000031243 RLG00000031247 RLG00000031248
rosa_multiflora	Rmu_sc0003758.1_g000015 Rmu_sc0005711.1_g000009 Rmu_sc0012347.1_g000002
rosa_roxburghii	Rroxscaffold_1G00070900 Rroxscaffold_1G00070960
rosa_rugosa	Rorug04G0412300 Rorug04G0412700 Rorug04G0412800 Rorug04G0412900 Rorug04G0413000
rosa_samantha	Rh1AG116200 Rh1DG120600 Rh5AG040400 Rh5BG039900 Rh5CG043700 Rh5CG044200 Rh5CG224800 Rh5DG039500 Rh5DG039600 Rh5DG040200
rosa_wichuraiana	Rw1G009340 Rw5G003880 Rw5G003920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	852
AccIII	TCCGGA	1 cut(s)	1096
AciI	CCGC	1 cut(s)	1201
AclWI	GGATC	1 cut(s)	1714
AcsI	RAATTY	2 cut(s)	164, 186
AcuI	CTGAAG	2 cut(s)	1380, 1509
AcvI	CACGTG	1 cut(s)	1355
AfaI	GTAC	6 cut(s)	90, 490, 950, 1125, 1253, 1412
AfiI	CCNNNNNNNGG	3 cut(s)	583, 627, 852
AflIII	ACRYGT	1 cut(s)	1352
AhlI	ACTAGT	1 cut(s)	1732
AluBI	AGCT	9 cut(s)	202, 557, 593, 682, 935, 1039, 1093, 1538, 1649
AluI	AGCT	9 cut(s)	202, 557, 593, 682, 935, 1039, 1093, 1538, 1649
Alw21I	GWGCWC	1 cut(s)	1704
Alw26I	GTCTC	1 cut(s)	872
AlwI	GGATC	1 cut(s)	1714
AlwNI	CAGNNNCTG	1 cut(s)	52
Ama87I	CYCGRG	1 cut(s)	1710
Aor13HI	TCCGGA	1 cut(s)	1096
AoxI	GGCC	4 cut(s)	292, 543, 1260, 1365
ApeKI	GCWGC	4 cut(s)	404, 407, 433, 1090
ApoI	RAATTY	2 cut(s)	164, 186
AspS9I	GGNCC	1 cut(s)	1365
AsuHPI	GGTGA	5 cut(s)	404, 578, 1042, 1490, 1703
AvaI	CYCGRG	1 cut(s)	1710
BaeGI	GKGCMC	1 cut(s)	1717
BauI	CACGAG	1 cut(s)	1747
BbrPI	CACGTG	1 cut(s)	1355
Bbv12I	GWGCWC	1 cut(s)	1704
BbvI	GCAGC	4 cut(s)	391, 394, 445, 1102
BccI	CCATC	5 cut(s)	164, 469, 694, 1670, 1676
BceAI	ACGGC	1 cut(s)	908
BcgI	CGANNNNNNTGC	2 cut(s)	1691, 1725
BciVI	GTATCC	1 cut(s)	853
BclI	TGATCA	2 cut(s)	94, 1693
BcoDI	GTCTC	1 cut(s)	872
BcuI	ACTAGT	1 cut(s)	1732
BfaI	CTAG	8 cut(s)	9, 290, 332, 554, 578, 627, 863, 1733
BfuI	GTATCC	1 cut(s)	853
BglII	AGATCT	3 cut(s)	829, 1110, 1402
BisI	GCNGC	4 cut(s)	405, 408, 434, 1091
BlpI	GCTNAGC	1 cut(s)	1464
BlsI	GCNGC	4 cut(s)	406, 409, 435, 1092
BmcAI	AGTACT	1 cut(s)	1125
BmeT110I	CYCGRG	1 cut(s)	1710
BmgT120I	GGNCC	1 cut(s)	1365
BmiI	GGNNCC	2 cut(s)	1181, 1366
BmsI	GCATC	1 cut(s)	288
BplI	GAGNNNNNCTC	2 cut(s)	381, 413
BpmI	CTGGAG	1 cut(s)	698
Bpu10I	CCTNAGC	1 cut(s)	1289
Bpu1102I	GCTNAGC	1 cut(s)	1464
BpuEI	CTTGAG	2 cut(s)	15, 800
Bsa29I	ATCGAT	1 cut(s)	1002
BsaAI	YACGTR	1 cut(s)	1355
BsaI	GGTCTC	1 cut(s)	872
BsaJI	CCNNGG	1 cut(s)	924
BsaWI	WCCGGW	1 cut(s)	1096
BsaXI	ACNNNNNCTCC	2 cut(s)	1232, 1262
Bsc4I	CCNNNNNNNGG	3 cut(s)	583, 627, 852
Bse1I	ACTGG	2 cut(s)	681, 906
Bse3DI	GCAATG	3 cut(s)	62, 1300, 1374
BseAI	TCCGGA	1 cut(s)	1096
BseCI	ATCGAT	1 cut(s)	1002
BseDI	CCNNGG	1 cut(s)	924
BseGI	GGATG	3 cut(s)	279, 484, 520
BseLI	CCNNNNNNNGG	3 cut(s)	583, 627, 852
BseMI	GCAATG	3 cut(s)	62, 1300, 1374
BseMII	CTCAG	3 cut(s)	625, 936, 1455
BseNI	ACTGG	2 cut(s)	681, 906
BseRI	GAGGAG	3 cut(s)	596, 1178, 1181
BseSI	GKGCMC	1 cut(s)	1717
BseXI	GCAGC	4 cut(s)	391, 394, 445, 1102
BsgI	GTGCAG	3 cut(s)	393, 722, 1373
BshFI	GGCC	4 cut(s)	294, 545, 1262, 1367
BshVI	ATCGAT	1 cut(s)	1002
BsiHKAI	GWGCWC	1 cut(s)	1704
BsiHKCI	CYCGRG	1 cut(s)	1710
BsiSI	CCGG	1 cut(s)	1097
BslFI	GGGAC	1 cut(s)	1720
BslI	CCNNNNNNNGG	3 cut(s)	583, 627, 852
BsmAI	GTCTC	1 cut(s)	872
BsmFI	GGGAC	1 cut(s)	1720
BsmI	GAATGC	2 cut(s)	531, 1525
BsnI	GGCC	4 cut(s)	294, 545, 1262, 1367
Bso31I	GGTCTC	1 cut(s)	872
BsoBI	CYCGRG	1 cut(s)	1710
Bsp1286I	GDGCHC	2 cut(s)	1704, 1717
Bsp13I	TCCGGA	1 cut(s)	1096
Bsp1407I	TGTACA	2 cut(s)	88, 1410
Bsp143I	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
Bsp1720I	GCTNAGC	1 cut(s)	1464
BspACI	CCGC	1 cut(s)	1201
BspANI	GGCC	4 cut(s)	294, 545, 1262, 1367
BspCNI	CTCAG	3 cut(s)	624, 937, 1456
BspDI	ATCGAT	1 cut(s)	1002
BspEI	TCCGGA	1 cut(s)	1096
BspLI	GGNNCC	2 cut(s)	1181, 1366
BspPI	GGATC	1 cut(s)	1714
BspQI	GCTCTTC	1 cut(s)	1029
BspTNI	GGTCTC	1 cut(s)	872
BsrDI	GCAATG	3 cut(s)	62, 1300, 1374
BsrGI	TGTACA	2 cut(s)	88, 1410
BsrI	ACTGG	2 cut(s)	681, 906
BssECI	CCNNGG	1 cut(s)	924
BssMI	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
BssSI	CACGAG	1 cut(s)	1747
Bst2BI	CACGAG	1 cut(s)	1747
Bst4CI	ACNGT	1 cut(s)	1272
Bst6I	CTCTTC	1 cut(s)	1029
BstAPI	GCANNNNNTGC	1 cut(s)	1299
BstAUI	TGTACA	2 cut(s)	88, 1410
BstBAI	YACGTR	1 cut(s)	1355
BstC8I	GCNNGC	1 cut(s)	1323
BstDEI	CTNAG	6 cut(s)	428, 611, 833, 945, 1289, 1464
BstDSI	CCRYGG	1 cut(s)	924
BstENI	CCTNNNNNAGG	1 cut(s)	581
BstF5I	GGATG	3 cut(s)	279, 484, 520
BstKTI	GATC	8 cut(s)	97, 397, 832, 1113, 1405, 1447, 1696, 1722
BstMAI	GTCTC	1 cut(s)	872
BstMBI	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
BstMWI	GCNNNNNNNGC	2 cut(s)	1299, 1364
BstNSI	RCATGY	1 cut(s)	665
BstSLI	GKGCMC	1 cut(s)	1717
BstV1I	GCAGC	4 cut(s)	391, 394, 445, 1102
BstX2I	RGATCY	4 cut(s)	829, 1110, 1402, 1719
BstXI	CCANNNNNNTGG	1 cut(s)	129
BstYI	RGATCY	4 cut(s)	829, 1110, 1402, 1719
Bsu15I	ATCGAT	1 cut(s)	1002
BsuI	GTATCC	1 cut(s)	853
BsuRI	GGCC	4 cut(s)	294, 545, 1262, 1367
BsuTUI	ATCGAT	1 cut(s)	1002
BtgI	CCRYGG	1 cut(s)	924
BtsCI	GGATG	3 cut(s)	279, 484, 520
BtsI	GCAGTG	1 cut(s)	710
BtsIMutI	CAGTG	2 cut(s)	674, 710
Cac8I	GCNNGC	1 cut(s)	1323
CaiI	CAGNNNCTG	1 cut(s)	52
Cfr13I	GGNCC	1 cut(s)	1365
ClaI	ATCGAT	1 cut(s)	1002
Csp6I	GTAC	6 cut(s)	89, 489, 949, 1124, 1252, 1411
CspCI	CAANNNNNGTGG	2 cut(s)	760, 795
CviQI	GTAC	6 cut(s)	89, 489, 949, 1124, 1252, 1411
DdeI	CTNAG	6 cut(s)	428, 611, 833, 945, 1289, 1464
DpnI	GATC	8 cut(s)	96, 396, 831, 1112, 1404, 1446, 1695, 1721
DpnII	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
Eam1104I	CTCTTC	1 cut(s)	1029
EarI	CTCTTC	1 cut(s)	1029
Eco147I	AGGCCT	3 cut(s)	294, 545, 1262
Eco31I	GGTCTC	1 cut(s)	872
Eco57I	CTGAAG	2 cut(s)	1380, 1509
Eco72I	CACGTG	1 cut(s)	1355
Eco88I	CYCGRG	1 cut(s)	1710
EcoNI	CCTNNNNNAGG	1 cut(s)	581
EcoRI	GAATTC	1 cut(s)	186
EcoT22I	ATGCAT	1 cut(s)	1573
FalI	AAGNNNNNCTT	4 cut(s)	513, 545, 1272, 1304
FaqI	GGGAC	1 cut(s)	1720
FbaI	TGATCA	2 cut(s)	94, 1693
Fnu4HI	GCNGC	4 cut(s)	405, 408, 434, 1091
FokI	GGATG	3 cut(s)	266, 491, 527
Fsp4HI	GCNGC	4 cut(s)	405, 408, 434, 1091
FspBI	CTAG	8 cut(s)	9, 290, 332, 554, 578, 627, 863, 1733
GluI	GCNGC	4 cut(s)	405, 408, 434, 1091
GsuI	CTGGAG	1 cut(s)	698
HaeIII	GGCC	4 cut(s)	294, 545, 1262, 1367
HapII	CCGG	1 cut(s)	1097
HindIII	AAGCTT	1 cut(s)	591
HinfI	GANTC	8 cut(s)	5, 149, 193, 242, 424, 1330, 1434, 1708
HpaII	CCGG	1 cut(s)	1097
HphI	GGTGA	5 cut(s)	404, 578, 1042, 1490, 1703
Hpy166II	GTNNAC	1 cut(s)	1411
Hpy188I	TCNGA	7 cut(s)	73, 192, 198, 247, 614, 1528, 1653
Hpy188III	TCNNGA	6 cut(s)	322, 387, 1097, 1673, 1697, 1749
Hpy8I	GTNNAC	1 cut(s)	1411
HpyAV	CCTTC	3 cut(s)	557, 1348, 1355
HpyCH4III	ACNGT	1 cut(s)	1272
HpyCH4IV	ACGT	2 cut(s)	1008, 1354
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1299, 1364
HpyF3I	CTNAG	6 cut(s)	428, 611, 833, 945, 1289, 1464
HpySE526I	ACGT	2 cut(s)	1008, 1354
Kpn2I	TCCGGA	1 cut(s)	1096
Ksp22I	TGATCA	2 cut(s)	94, 1693
Kzo9I	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
LguI	GCTCTTC	1 cut(s)	1029
LmnI	GCTCC	1 cut(s)	679
LpnPI	CCDG	8 cut(s)	308, 662, 887, 957, 1110, 1307, 1433, 1623
Lsp1109I	GCAGC	4 cut(s)	391, 394, 445, 1102
LweI	GCATC	1 cut(s)	288
MaeI	CTAG	8 cut(s)	9, 290, 332, 554, 578, 627, 863, 1733
MaeII	ACGT	2 cut(s)	1008, 1354
MaeIII	GTNAC	3 cut(s)	440, 604, 1502
MalI	GATC	8 cut(s)	96, 396, 831, 1112, 1404, 1446, 1695, 1721
MboI	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
MfeI	CAATTG	1 cut(s)	646
MflI	RGATCY	4 cut(s)	829, 1110, 1402, 1719
MhlI	GDGCHC	2 cut(s)	1704, 1717
MluCI	AATT	8 cut(s)	112, 164, 186, 646, 985, 990, 1146, 1158
MlyI	GAGTC	3 cut(s)	1324, 1428, 1702
MmeI	TCCRAC	3 cut(s)	243, 519, 1157
Mph1103I	ATGCAT	1 cut(s)	1573
MroI	TCCGGA	1 cut(s)	1096
MslI	CAYNNNNRTG	2 cut(s)	1395, 1604
MspI	CCGG	1 cut(s)	1097
MunI	CAATTG	1 cut(s)	646
Mva1269I	GAATGC	2 cut(s)	531, 1525
MwoI	GCNNNNNNNGC	2 cut(s)	1299, 1364
NdeII	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
NlaIV	GGNNCC	2 cut(s)	1181, 1366
NmuCI	GTSAC	1 cut(s)	604
NsiI	ATGCAT	1 cut(s)	1573
NspI	RCATGY	1 cut(s)	665
PceI	AGGCCT	3 cut(s)	294, 545, 1262
PciSI	GCTCTTC	1 cut(s)	1029
PctI	GAATGC	2 cut(s)	531, 1525
PfeI	GAWTC	5 cut(s)	5, 149, 193, 242, 424
PflMI	CCANNNNNTGG	1 cut(s)	852
PkrI	GCNGC	4 cut(s)	406, 409, 435, 1092
PleI	GAGTC	3 cut(s)	1324, 1428, 1702
PmaCI	CACGTG	1 cut(s)	1355
PmlI	CACGTG	1 cut(s)	1355
PpsI	GAGTC	3 cut(s)	1324, 1428, 1702
Ppu21I	YACGTR	1 cut(s)	1355
PspCI	CACGTG	1 cut(s)	1355
PspN4I	GGNNCC	2 cut(s)	1181, 1366
PspPI	GGNCC	1 cut(s)	1365
PstNI	CAGNNNCTG	1 cut(s)	52
PsuI	RGATCY	4 cut(s)	829, 1110, 1402, 1719
RsaI	GTAC	6 cut(s)	90, 490, 950, 1125, 1253, 1412
RsaNI	GTAC	6 cut(s)	89, 489, 949, 1124, 1252, 1411
RseI	CAYNNNNRTG	2 cut(s)	1395, 1604
SapI	GCTCTTC	1 cut(s)	1029
SatI	GCNGC	4 cut(s)	405, 408, 434, 1091
Sau3AI	GATC	8 cut(s)	94, 394, 829, 1110, 1402, 1444, 1693, 1719
Sau96I	GGNCC	1 cut(s)	1365
ScaI	AGTACT	1 cut(s)	1125
SchI	GAGTC	3 cut(s)	1324, 1428, 1702
SduI	GDGCHC	2 cut(s)	1704, 1717
SfaNI	GCATC	1 cut(s)	288
SmiMI	CAYNNNNRTG	2 cut(s)	1395, 1604
SmlI	CTYRAG	2 cut(s)	30, 779
SmoI	CTYRAG	2 cut(s)	30, 779
SpeI	ACTAGT	1 cut(s)	1732
Sse9I	AATT	8 cut(s)	112, 164, 186, 646, 985, 990, 1146, 1158
SseBI	AGGCCT	3 cut(s)	294, 545, 1262
SsiI	CCGC	1 cut(s)	1201
SspI	AATATT	1 cut(s)	127
SspMI	CTAG	8 cut(s)	9, 290, 332, 554, 578, 627, 863, 1733
StuI	AGGCCT	3 cut(s)	294, 545, 1262
TaaI	ACNGT	1 cut(s)	1272
TaiI	ACGT	2 cut(s)	1011, 1357
TaqI	TCGA	7 cut(s)	147, 184, 357, 939, 1002, 1432, 1540
TasI	AATT	8 cut(s)	112, 164, 186, 646, 985, 990, 1146, 1158
TatI	WGTACW	4 cut(s)	88, 948, 1123, 1410
TfiI	GAWTC	5 cut(s)	5, 149, 193, 242, 424
TscAI	CASTG	2 cut(s)	681, 710
TseFI	GTSAC	1 cut(s)	604
TseI	GCWGC	4 cut(s)	404, 407, 433, 1090
Tsp45I	GTSAC	1 cut(s)	604
TspDTI	ATGAA	8 cut(s)	495, 904, 1121, 1170, 1296, 1413, 1479, 1611
TspRI	CASTG	2 cut(s)	681, 710
Van91I	CCANNNNNTGG	1 cut(s)	852
XagI	CCTNNNNNAGG	1 cut(s)	581
XapI	RAATTY	2 cut(s)	164, 186
XceI	RCATGY	1 cut(s)	665
XspI	CTAG	8 cut(s)	9, 290, 332, 554, 578, 627, 863, 1733
ZrmI	AGTACT	1 cut(s)	1125
Zsp2I	ATGCAT	1 cut(s)	1573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.