RchiOBHm_Chr5g0004631

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002039
Physical Location & Seq
Forward (+)
3017005 .. 3019500
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28586

Sequence Viewer

Length: 1752 bp
ATGGATTGTGGTATGCACTTGCATAACCATCAAGCTGATGAGCAACAGATACTGCAATGGCAGATGAAATCCGAACCCCCTTCCTTGGTACATGATCAAAGACGATCTGCCAATTACAAGCCGAATATTTGGAAGTATGATTTCCTTGAATCTCTTAACAGCAAATTTGATGGAGAATGTTATCGAATTCAGATTCAGAAGCTAATAGAAGATGTTAAGGATTTGTTTGGTGAATGTAAGGAATCATGTTCACTGGGTAAGTTGAAGTTGATTGACAGCATCCAGAAACTAGGCCTCAACAACCACTTTGAGAAGGAAATCAAGGAAGCCCTAGACACCATAGCATCTGTAGAACTCGAAAACAATAGCAATCCCTGCATTAGTTCTGAGGGTGATCTCTATGCTACTGCACTACTCTTTAAGATTCTTAGGCAGCATGGTTATAAAGTTTCACAAGATATATTTGGTAGTTTGATAGATGAAATGGGTACATTAAAGAAGAACACATGTGGGGATGCGAAAGGAATGCTTGAAATGGTGGAGGCCTCAAACTTAGCTTTAGAAGGTGAAAATATCCTAGAGGAGGCTAAAGCTTTCTTAATGGTCACTCTCAGAGATACCAATACTAGGTGTGATGATATAGACAATTGCATTTCCAAGCATGTGACTTATGCCCTGGAGTTTCCATCACAAAGGAGAGTGCAGTGGTTTAATGTGAAATGGCACATCAAATCCTACGAGAAAGACCATACTGATCAAGCCAACATGACCACTTTACTAGAGTTGGCTAAACTTAACTTCAACATAGTTCAAGCCACACTTCAAAAAGATCTAAGGGAAGTATCCATGTGGTGGAACAATCTGGGTCTCAATAAGAACTTGGACTTTGCAAGAGATAGACTGGTCGAATGTTTCATGTGTGCTGTGGGATTAGCTTTCGAGCCTCAGTACAAGTCCTTTAGAAAATGGATAACTAAAGTCGTCAATTTGATTCTCATCATCGATGACGTTTATGATGTTTATGGCTCATTGGAAGAGCTAAAACATTTCACCAATGCTGTTGAAAGGTGGGATATTAGGGAAACCGAGCACCTTCCGGAGTGTATGAAGATCTGTTTCCAAGTACTCTACAATACTATTTGTGAAATTGCTTATGAAATTGAGGAGGAGAATGGTTGGAACCAAGTATTACCTCATTTGCAGAAAGTGTGGGGAGAGTTCTGTAAAGCATTATTGGTGGACGCAGAGTGGTACAATAAGGCCTACACACCGTCCTTTGAAGCGCAGCTTAGCAATGGTTGCATTTCATCATCTGCTTCGCTGATTTTGACTCATGCCTTCTTCTCCACAACACATGATGAAGGGGCCAAAAACATTACTGATTTTCTGCACAAGAATGAAGATCTTGTGTACAATATCTCTTTGATACTTCGACTCTTAAACGATTTGGGAACTTCCGCGGCTGAGCAAGAGAGAGGTGATGCTGCTTCATCAATCCTATGTTACATGAAAGAAGCGAATGCTTCAGAAGAAATAGCTCGAAAGCACATCAAAGACATGATAGACAATGCATGGAAGAAAATAAACAAAAAATGCTTCACTCAAATGCCCGAAATATCTTCATTCATCAACATTATGACAAATATAGCTCGAGTTGGACATAGCCTTTATCAAGATGGAGATGGATTTGGTGATCAAGAGTACGGGACTCGGGGACAGATCCAATCTTTACTAGTTGAACCTCTCTCTTAA

Protein Analysis

583

Amino Acids

67.0

Weight (kDa)

5.14

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 43 - 226 2.1e-42 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 287 - 527 1e-86 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 326 - 527 7.5e-52 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 852
AccII CGCG 1 cut(s) 1460
AccIII TCCGGA 1 cut(s) 1096
AciI CCGC 2 cut(s) 1458, 1460
AclWI GGATC 1 cut(s) 1714
AcsI RAATTY 2 cut(s) 164, 186
AcuI CTGAAG 1 cut(s) 1509
AfaI GTAC 7 cut(s) 90, 490, 950, 1125, 1253, 1412, 1703
AfiI CCNNNNNNNGG 4 cut(s) 85, 583, 627, 852
AflIII ACRYGT 1 cut(s) 506
AgsI TTSAA 9 cut(s) 149, 265, 533, 802, 812, 824, 1064, 1280, 1739
AhlI ACTAGT 1 cut(s) 1732
AjnI CCWGG 1 cut(s) 675
AluBI AGCT 9 cut(s) 35, 202, 557, 593, 935, 1039, 1288, 1538, 1649
AluI AGCT 9 cut(s) 35, 202, 557, 593, 935, 1039, 1288, 1538, 1649
Alw21I GWGCWC 1 cut(s) 1092
Alw26I GTCTC 1 cut(s) 872
AlwI GGATC 1 cut(s) 1714
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 2 cut(s) 1650, 1710
Aor13HI TCCGGA 1 cut(s) 1096
AoxI GGCC 4 cut(s) 292, 543, 1260, 1365
ApeKI GCWGC 3 cut(s) 433, 1285, 1484
ApoI RAATTY 2 cut(s) 164, 186
AspLEI GCGC 1 cut(s) 1285
AspS9I GGNCC 1 cut(s) 1365
AsuHPI GGTGA 6 cut(s) 242, 404, 578, 1042, 1490, 1703
AvaI CYCGRG 2 cut(s) 1650, 1710
Bbv12I GWGCWC 1 cut(s) 1092
BbvI GCAGC 3 cut(s) 445, 1297, 1471
BccI CCATC 5 cut(s) 36, 164, 694, 1670, 1676
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 1 cut(s) 677
BciVI GTATCC 1 cut(s) 853
BclI TGATCA 3 cut(s) 94, 754, 1693
BcoDI GTCTC 1 cut(s) 872
BcuI ACTAGT 1 cut(s) 1732
BfaI CTAG 6 cut(s) 290, 332, 578, 627, 779, 1733
BfmI CTRYAG 1 cut(s) 348
BfuI GTATCC 1 cut(s) 853
BglII AGATCT 3 cut(s) 829, 1110, 1402
BisI GCNGC 4 cut(s) 434, 1286, 1461, 1485
BlpI GCTNAGC 2 cut(s) 1289, 1464
BlsI GCNGC 4 cut(s) 435, 1287, 1462, 1486
BmcAI AGTACT 1 cut(s) 1125
Bme1390I CCNGG 1 cut(s) 677
BmeT110I CYCGRG 2 cut(s) 1650, 1710
BmgT120I GGNCC 1 cut(s) 1365
BmiI GGNNCC 2 cut(s) 1181, 1366
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 4 cut(s) 288, 353, 505, 1471
BmuI ACTGGG 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 698
Bpu1102I GCTNAGC 2 cut(s) 1289, 1464
Bsa29I ATCGAT 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 995
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 3 cut(s) 84, 675, 1458
BsaWI WCCGGW 1 cut(s) 1096
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 583, 627, 852
Bse1I ACTGG 2 cut(s) 258, 906
Bse3DI GCAATG 2 cut(s) 62, 1300
Bse8I GATNNNNATC 1 cut(s) 995
BseAI TCCGGA 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 677
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 3 cut(s) 84, 675, 1458
BseGI GGATG 2 cut(s) 279, 520
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 4 cut(s) 85, 583, 627, 852
BseMI GCAATG 2 cut(s) 62, 1300
BseMII CTCAG 4 cut(s) 378, 625, 959, 1455
BseNI ACTGG 2 cut(s) 258, 906
BseRI GAGGAG 3 cut(s) 596, 1178, 1181
BseXI GCAGC 3 cut(s) 445, 1297, 1471
BsgI GTGCAG 3 cut(s) 393, 722, 1373
Bsh1236I CGCG 1 cut(s) 1460
BshFI GGCC 4 cut(s) 294, 545, 1262, 1367
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 1092
BsiHKCI CYCGRG 2 cut(s) 1650, 1710
BsiSI CCGG 1 cut(s) 1097
BslFI GGGAC 2 cut(s) 1720, 1728
BslI CCNNNNNNNGG 4 cut(s) 85, 583, 627, 852
BsmAI GTCTC 1 cut(s) 872
BsmFI GGGAC 2 cut(s) 1720, 1728
BsmI GAATGC 2 cut(s) 531, 1525
BsnI GGCC 4 cut(s) 294, 545, 1262, 1367
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 2 cut(s) 1650, 1710
Bsp1286I GDGCHC 1 cut(s) 1092
Bsp13I TCCGGA 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 1410
Bsp143I GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
Bsp1720I GCTNAGC 2 cut(s) 1289, 1464
BspACI CCGC 2 cut(s) 1458, 1460
BspANI GGCC 4 cut(s) 294, 545, 1262, 1367
BspCNI CTCAG 4 cut(s) 379, 624, 958, 1456
BspDI ATCGAT 1 cut(s) 1002
BspEI TCCGGA 1 cut(s) 1096
BspFNI CGCG 1 cut(s) 1460
BspLI GGNNCC 2 cut(s) 1181, 1366
BspPI GGATC 1 cut(s) 1714
BspQI GCTCTTC 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 2 cut(s) 62, 1300
BsrGI TGTACA 1 cut(s) 1410
BsrI ACTGG 2 cut(s) 258, 906
BssECI CCNNGG 3 cut(s) 84, 675, 1458
BssMI GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 1 cut(s) 1272
Bst6I CTCTTC 1 cut(s) 1029
BstAPI GCANNNNNTGC 2 cut(s) 375, 1299
BstAUI TGTACA 1 cut(s) 1410
BstDEI CTNAG 8 cut(s) 387, 428, 553, 611, 833, 945, 1289, 1464
BstDSI CCRYGG 1 cut(s) 1458
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 2 cut(s) 279, 520
BstFNI CGCG 1 cut(s) 1460
BstHHI GCGC 1 cut(s) 1285
BstKTI GATC 9 cut(s) 97, 107, 397, 757, 832, 1113, 1405, 1696, 1722
BstMAI GTCTC 1 cut(s) 872
BstMBI GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
BstMWI GCNNNNNNNGC 2 cut(s) 375, 1299
BstNI CCWGG 1 cut(s) 677
BstNSI RCATGY 2 cut(s) 510, 665
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 348
BstUI CGCG 1 cut(s) 1460
BstV1I GCAGC 3 cut(s) 445, 1297, 1471
BstX2I RGATCY 4 cut(s) 829, 1110, 1402, 1719
BstYI RGATCY 4 cut(s) 829, 1110, 1402, 1719
Bsu15I ATCGAT 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 4 cut(s) 294, 545, 1262, 1367
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 1 cut(s) 1458
BtsCI GGATG 2 cut(s) 279, 520
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 2 cut(s) 251, 710
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 1285
Cfr13I GGNCC 1 cut(s) 1365
Cfr42I CCGCGG 1 cut(s) 1461
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 1 cut(s) 1250
Csp6I GTAC 7 cut(s) 89, 489, 949, 1124, 1252, 1411, 1702
CviQI GTAC 7 cut(s) 89, 489, 949, 1124, 1252, 1411, 1702
DdeI CTNAG 8 cut(s) 387, 428, 553, 611, 833, 945, 1289, 1464
DpnI GATC 9 cut(s) 96, 106, 396, 756, 831, 1112, 1404, 1695, 1721
DpnII GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
Eam1104I CTCTTC 1 cut(s) 1029
EarI CTCTTC 1 cut(s) 1029
Eco130I CCWWGG 1 cut(s) 84
Eco147I AGGCCT 3 cut(s) 294, 545, 1262
Eco31I GGTCTC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 1509
Eco88I CYCGRG 2 cut(s) 1650, 1710
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 1573
ErhI CCWWGG 1 cut(s) 84
FalI AAGNNNNNCTT 6 cut(s) 513, 545, 804, 836, 1272, 1304
FaqI GGGAC 2 cut(s) 1720, 1728
FbaI TGATCA 3 cut(s) 94, 754, 1693
Fnu4HI GCNGC 4 cut(s) 434, 1286, 1461, 1485
FokI GGATG 2 cut(s) 266, 527
Fsp4HI GCNGC 4 cut(s) 434, 1286, 1461, 1485
FspBI CTAG 6 cut(s) 290, 332, 578, 627, 779, 1733
GlaI GCGC 1 cut(s) 1284
GluI GCNGC 4 cut(s) 434, 1286, 1461, 1485
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 4 cut(s) 294, 545, 1262, 1367
HapII CCGG 1 cut(s) 1097
HgaI GACGC 1 cut(s) 1250
HhaI GCGC 1 cut(s) 1285
Hin6I GCGC 1 cut(s) 1283
HinP1I GCGC 1 cut(s) 1283
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 8 cut(s) 149, 193, 242, 424, 991, 1330, 1434, 1708
HpaII CCGG 1 cut(s) 1097
HphI GGTGA 6 cut(s) 242, 404, 578, 1042, 1490, 1703
Hpy166II GTNNAC 3 cut(s) 251, 1240, 1411
Hpy188I TCNGA 6 cut(s) 73, 192, 198, 388, 614, 1528
Hpy188III TCNNGA 4 cut(s) 283, 1097, 1673, 1697
Hpy8I GTNNAC 3 cut(s) 251, 1240, 1411
HpyAV CCTTC 6 cut(s) 90, 307, 557, 1103, 1348, 1355
HpyCH4III ACNGT 1 cut(s) 1272
HpyCH4IV ACGT 1 cut(s) 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 1299
HpyF3I CTNAG 8 cut(s) 387, 428, 553, 611, 833, 945, 1289, 1464
HpySE526I ACGT 1 cut(s) 1008
HspAI GCGC 1 cut(s) 1283
Kpn2I TCCGGA 1 cut(s) 1096
Ksp22I TGATCA 3 cut(s) 94, 754, 1693
KspI CCGCGG 1 cut(s) 1461
Kzo9I GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
LguI GCTCTTC 1 cut(s) 1029
LpnPI CCDG 8 cut(s) 239, 296, 388, 662, 689, 848, 887, 1110
Lsp1109I GCAGC 3 cut(s) 445, 1297, 1471
LweI GCATC 4 cut(s) 288, 353, 505, 1471
MaeI CTAG 6 cut(s) 290, 332, 578, 627, 779, 1733
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 3 cut(s) 604, 664, 1502
MalI GATC 9 cut(s) 96, 106, 396, 756, 831, 1112, 1404, 1695, 1721
MboI GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
MboII GAAGA 9 cut(s) 221, 511, 1046, 1120, 1333, 1412, 1541, 1588, 1611
MfeI CAATTG 1 cut(s) 646
MflI RGATCY 4 cut(s) 829, 1110, 1402, 1719
MhlI GDGCHC 1 cut(s) 1092
MluCI AATT 7 cut(s) 112, 164, 186, 646, 985, 1146, 1158
MlyI GAGTC 3 cut(s) 1324, 1428, 1702
MmeI TCCRAC 2 cut(s) 1157, 1636
Mph1103I ATGCAT 1 cut(s) 1573
MroI TCCGGA 1 cut(s) 1096
MseI TTAA 9 cut(s) 156, 216, 420, 494, 599, 711, 795, 1439, 1750
MslI CAYNNNNRTG 2 cut(s) 1395, 1604
MspA1I CMGCKG 1 cut(s) 1460
MspI CCGG 1 cut(s) 1097
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 646
Mva1269I GAATGC 2 cut(s) 531, 1525
MvaI CCWGG 1 cut(s) 677
MvnI CGCG 1 cut(s) 1460
MwoI GCNNNNNNNGC 2 cut(s) 375, 1299
NdeII GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
NlaIV GGNNCC 2 cut(s) 1181, 1366
NmuCI GTSAC 2 cut(s) 604, 664
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 2 cut(s) 510, 665
PaeR7I CTCGAG 1 cut(s) 1650
PceI AGGCCT 3 cut(s) 294, 545, 1262
PciI ACATGT 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 1029
PctI GAATGC 2 cut(s) 531, 1525
PfeI GAWTC 5 cut(s) 149, 193, 242, 424, 991
PflMI CCANNNNNTGG 1 cut(s) 852
PkrI GCNGC 4 cut(s) 435, 1287, 1462, 1486
PleI GAGTC 3 cut(s) 1324, 1428, 1702
PpsI GAGTC 3 cut(s) 1324, 1428, 1702
PscI ACATGT 1 cut(s) 506
PsiI TTATAA 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 2 cut(s) 1181, 1366
PspPI GGNCC 1 cut(s) 1365
PspXI VCTCGAGB 1 cut(s) 1650
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 4 cut(s) 829, 1110, 1402, 1719
RsaI GTAC 7 cut(s) 90, 490, 950, 1125, 1253, 1412, 1703
RsaNI GTAC 7 cut(s) 89, 489, 949, 1124, 1252, 1411, 1702
RseI CAYNNNNRTG 2 cut(s) 1395, 1604
SacII CCGCGG 1 cut(s) 1461
SapI GCTCTTC 1 cut(s) 1029
SaqAI TTAA 9 cut(s) 156, 216, 420, 494, 599, 711, 795, 1439, 1750
SatI GCNGC 4 cut(s) 434, 1286, 1461, 1485
Sau3AI GATC 9 cut(s) 94, 104, 394, 754, 829, 1110, 1402, 1693, 1719
Sau96I GGNCC 1 cut(s) 1365
ScaI AGTACT 1 cut(s) 1125
SchI GAGTC 3 cut(s) 1324, 1428, 1702
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 1 cut(s) 1092
SfaNI GCATC 4 cut(s) 288, 353, 505, 1471
SfcI CTRYAG 1 cut(s) 348
Sfr274I CTCGAG 1 cut(s) 1650
Sfr303I CCGCGG 1 cut(s) 1461
SgrBI CCGCGG 1 cut(s) 1461
SlaI CTCGAG 1 cut(s) 1650
SmiMI CAYNNNNRTG 2 cut(s) 1395, 1604
SmlI CTYRAG 1 cut(s) 1650
SmoI CTYRAG 1 cut(s) 1650
SpeI ACTAGT 1 cut(s) 1732
Sse9I AATT 7 cut(s) 112, 164, 186, 646, 985, 1146, 1158
SseBI AGGCCT 3 cut(s) 294, 545, 1262
SsiI CCGC 2 cut(s) 1458, 1460
SspI AATATT 1 cut(s) 127
SspMI CTAG 6 cut(s) 290, 332, 578, 627, 779, 1733
StuI AGGCCT 3 cut(s) 294, 545, 1262
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 1272
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 8 cut(s) 184, 357, 906, 939, 1002, 1432, 1540, 1651
TasI AATT 7 cut(s) 112, 164, 186, 646, 985, 1146, 1158
TatI WGTACW 3 cut(s) 948, 1123, 1410
TauI GCSGC 1 cut(s) 1463
TfiI GAWTC 5 cut(s) 149, 193, 242, 424, 991
Tru1I TTAA 9 cut(s) 156, 216, 420, 494, 599, 711, 795, 1439, 1750
Tru9I TTAA 9 cut(s) 156, 216, 420, 494, 599, 711, 795, 1439, 1750
TscAI CASTG 2 cut(s) 258, 710
TseFI GTSAC 2 cut(s) 604, 664
TseI GCWGC 3 cut(s) 433, 1285, 1484
Tsp45I GTSAC 2 cut(s) 604, 664
TspRI CASTG 2 cut(s) 258, 710
Van91I CCANNNNNTGG 1 cut(s) 852
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 2 cut(s) 164, 186
XceI RCATGY 2 cut(s) 510, 665
XhoI CTCGAG 1 cut(s) 1650
XspI CTAG 6 cut(s) 290, 332, 578, 627, 779, 1733
ZrmI AGTACT 1 cut(s) 1125
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.