Rorug04G0412300

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002039
Physical Location & Seq
Reverse (-)
57430732 .. 57432426
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0412300.1

Sequence Viewer

Length: 852 bp
ATGTCCGATCCACAAATTCTGACAACATTGGAAGAAGGTGCCGGCAGCTCGCCTCCGACTAAGAGCAGATTTTCTGGGCTTTGTTCATCTTCTAAGATTGTTCACCTTCTCCAAAAGCTAATTGCAGAGGTGATGGGTACATATTTCTTGATATTTGCCGGCTGTGGTTCGATAGTGGTGAACAAGATATATGGTTCAATAACATTTCCAGGGGTGGCTGTCGTCTGGGGCTTAACCGCTATGGTCATGATCTATTCCGTTGGTCATATCTCCGGCGCCCATTTTAATCCCGCCGTCACCATTACTTTTGCCATCTTTCGCGATTTTCCTCTCAAACAGTTACCTCTGTACATCGTGGCGCAGCTTTTAGGTTCCATACTTGCGAGTGGGACGTTATGCCTTTGTTTCACCGTACATCCGGATGATTTCTTTGGAACTGTACCGGTTGGATCATATGTTCAATCTTTGGTCATTGAAATCATTGCCTCATTTCTCTTGATGTTCGTCATTTCCGGCGTTGCCACAGATAATAGAGCTATTGGCCAAATGGCAGGAATTGCTATAGGAATGACCATATTAGTCGATGTCTTCGTTTGCGGGCCTGTATCAGGGGCGTCGATGAACCCAGCTCGGAGCATTGGGCCGGCATTGATTATGAAGAACTTTAAGGGACTCTGGGTTTACATTGTAGGTCCAATCATTGGGACAATATTGGGTGGATTTGCTTACAATTTGATAAGACGCACCGACAAACCACTCCGGGAAATAACGAAGAGCGCTTCCATGTCGATCAAGAGCGTTCCCATCCTCAAGTCCATGTCCAGGAACACCACTTTCAACAGTAGAACCTAA

Protein Analysis

283

Amino Acids

30.2

Weight (kDa)

9.38

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 33 - 243 9.8e-61 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 38, 275
AccB7I CCANNNNNTGG 1 cut(s) 701
AccII CGCG 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 3 cut(s) 237, 291, 597
AclWI GGATC 2 cut(s) 2, 457
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 1 cut(s) 15
AcyI GRCGYC 2 cut(s) 276, 614
AfaI GTAC 4 cut(s) 139, 350, 414, 441
AfeI AGCGCT 1 cut(s) 778
AfiI CCNNNNNNNGG 3 cut(s) 608, 701, 822
AgeI ACCGGT 1 cut(s) 442
AgsI TTSAA 4 cut(s) 198, 461, 476, 838
AjnI CCWGG 2 cut(s) 208, 821
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 5 cut(s) 48, 118, 364, 536, 629
AluI AGCT 5 cut(s) 48, 118, 364, 536, 629
AlwI GGATC 2 cut(s) 2, 457
Aor13HI TCCGGA 1 cut(s) 418
Aor51HI AGCGCT 1 cut(s) 778
AoxI GGCC 3 cut(s) 541, 599, 641
ApeKI GCWGC 2 cut(s) 45, 361
ApoI RAATTY 1 cut(s) 15
AsiGI ACCGGT 1 cut(s) 442
AspLEI GCGC 3 cut(s) 278, 361, 779
AspS9I GGNCC 3 cut(s) 599, 641, 692
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 5 cut(s) 95, 142, 190, 289, 400
AvaII GGWCC 1 cut(s) 692
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 2 cut(s) 38, 275
BbsI GAAGAC 1 cut(s) 580
BbvI GCAGC 2 cut(s) 57, 373
BccI CCATC 3 cut(s) 127, 320, 812
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 210, 823
BcnI CCSGG 1 cut(s) 761
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 2 cut(s) 279, 780
BisI GCNGC 2 cut(s) 46, 362
BlsI GCNGC 2 cut(s) 47, 363
Bme1390I CCNGG 3 cut(s) 210, 761, 823
Bme18I GGWCC 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 599, 641, 692
BmiI GGNNCC 3 cut(s) 40, 277, 373
BmrFI CCNGG 3 cut(s) 210, 761, 823
BpiI GAAGAC 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 761
BsaHI GRCGYC 2 cut(s) 276, 614
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 2 cut(s) 418, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 701, 822
Bse118I RCCGGY 4 cut(s) 41, 158, 442, 643
Bse3DI GCAATG 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 2 cut(s) 210, 823
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 3 cut(s) 415, 427, 804
BseLI CCNNNNNNNGG 3 cut(s) 608, 701, 822
BseMI GCAATG 1 cut(s) 480
BseXI GCAGC 2 cut(s) 57, 373
BseYI CCCAGC 1 cut(s) 625
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 3 cut(s) 543, 601, 643
BshNI GGYRCC 2 cut(s) 38, 275
BshTI ACCGGT 1 cut(s) 442
BsiSI CCGG 8 cut(s) 42, 159, 273, 419, 443, 513, 644, 760
BslFI GGGAC 3 cut(s) 403, 684, 718
BslI CCNNNNNNNGG 3 cut(s) 608, 701, 822
BsmFI GGGAC 3 cut(s) 403, 684, 718
BsnI GGCC 3 cut(s) 543, 601, 643
Bsp13I TCCGGA 1 cut(s) 418
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 4 cut(s) 7, 249, 449, 789
Bsp68I TCGCGA 1 cut(s) 321
BspACI CCGC 3 cut(s) 237, 291, 597
BspANI GGCC 3 cut(s) 543, 601, 643
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 3 cut(s) 40, 277, 373
BspPI GGATC 2 cut(s) 2, 457
BspQI GCTCTTC 1 cut(s) 767
BspT107I GGYRCC 2 cut(s) 38, 275
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 4 cut(s) 41, 158, 442, 643
BsrGI TGTACA 1 cut(s) 348
BssAI RCCGGY 4 cut(s) 41, 158, 442, 643
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 4 cut(s) 7, 249, 449, 789
BssNI GRCGYC 2 cut(s) 276, 614
Bst2UI CCWGG 2 cut(s) 210, 823
Bst4CI ACNGT 4 cut(s) 339, 412, 439, 842
Bst6I CTCTTC 1 cut(s) 767
BstACI GRCGYC 2 cut(s) 276, 614
BstAPI GCANNNNNTGC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 5 cut(s) 43, 50, 160, 599, 645
BstDEI CTNAG 2 cut(s) 60, 93
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 415, 427, 804
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 2 cut(s) 279, 780
BstHHI GCGC 3 cut(s) 278, 361, 779
BstKTI GATC 4 cut(s) 10, 252, 452, 792
BstMBI GATC 4 cut(s) 7, 249, 449, 789
BstMWI GCNNNNNNNGC 1 cut(s) 557
BstNI CCWGG 2 cut(s) 210, 823
BstSCI CCNGG 3 cut(s) 208, 759, 821
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 57, 373
BstV2I GAAGAC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 543, 601, 643
BtsCI GGATG 3 cut(s) 415, 427, 804
BtuMI TCGCGA 1 cut(s) 321
Cac8I GCNNGC 5 cut(s) 43, 50, 160, 599, 645
CciI TCATGA 1 cut(s) 246
CfoI GCGC 3 cut(s) 278, 361, 779
Cfr10I RCCGGY 4 cut(s) 41, 158, 442, 643
Cfr13I GGNCC 3 cut(s) 599, 641, 692
CseI GACGC 2 cut(s) 603, 750
Csp6I GTAC 4 cut(s) 138, 349, 413, 440
CspAI ACCGGT 1 cut(s) 442
CviAII CATG 3 cut(s) 247, 784, 817
CviQI GTAC 4 cut(s) 138, 349, 413, 440
DdeI CTNAG 2 cut(s) 60, 93
DinI GGCGCC 1 cut(s) 277
DpnI GATC 4 cut(s) 9, 251, 451, 791
DpnII GATC 4 cut(s) 7, 249, 449, 789
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 767
EarI CTCTTC 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 692
Eco47III AGCGCT 1 cut(s) 778
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 208, 821
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
FaeI CATG 3 cut(s) 250, 787, 820
FaqI GGGAC 3 cut(s) 403, 684, 718
FatI CATG 3 cut(s) 246, 783, 816
FauI CCCGC 2 cut(s) 298, 590
FauNDI CATATG 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 46, 362
FokI GGATG 3 cut(s) 402, 434, 791
Fsp4HI GCNGC 2 cut(s) 46, 362
GlaI GCGC 3 cut(s) 277, 360, 778
GluI GCNGC 2 cut(s) 46, 362
GsaI CCCAGC 1 cut(s) 629
HaeII RGCGCY 2 cut(s) 279, 780
HaeIII GGCC 3 cut(s) 543, 601, 643
HapII CCGG 8 cut(s) 42, 159, 273, 419, 443, 513, 644, 760
HgaI GACGC 2 cut(s) 603, 750
HhaI GCGC 3 cut(s) 278, 361, 779
Hin1I GRCGYC 2 cut(s) 276, 614
Hin1II CATG 3 cut(s) 250, 787, 820
Hin6I GCGC 3 cut(s) 276, 359, 777
HinP1I GCGC 3 cut(s) 276, 359, 777
HinfI GANTC 1 cut(s) 672
HpaII CCGG 8 cut(s) 42, 159, 273, 419, 443, 513, 644, 760
HphI GGTGA 5 cut(s) 95, 142, 190, 289, 400
Hpy166II GTNNAC 3 cut(s) 103, 181, 682
Hpy188I TCNGA 4 cut(s) 7, 21, 57, 633
Hpy188III TCNNGA 6 cut(s) 148, 247, 320, 419, 496, 793
Hpy8I GTNNAC 3 cut(s) 103, 181, 682
Hpy99I CGWCG 1 cut(s) 619
HpyAV CCTTC 2 cut(s) 29, 116
HpyCH4III ACNGT 4 cut(s) 339, 412, 439, 842
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 1 cut(s) 557
HpyF3I CTNAG 2 cut(s) 60, 93
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 2 cut(s) 276, 614
Hsp92II CATG 3 cut(s) 250, 787, 820
HspAI GCGC 3 cut(s) 276, 359, 777
KasI GGCGCC 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 418
KroI GCCGGC 3 cut(s) 41, 158, 643
KroNI GCCGGC 3 cut(s) 43, 160, 645
Kzo9I GATC 4 cut(s) 7, 249, 449, 789
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 1 cut(s) 633
Lsp1109I GCAGC 2 cut(s) 57, 373
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 295, 339
MalI GATC 4 cut(s) 9, 251, 451, 791
MboI GATC 4 cut(s) 7, 249, 449, 789
MboII GAAGA 5 cut(s) 44, 81, 580, 670, 784
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 4 cut(s) 15, 120, 555, 730
MluNI TGGCCA 1 cut(s) 543
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 666
MmeI TCCRAC 2 cut(s) 80, 427
MnlI CCTC 6 cut(s) 63, 121, 339, 354, 496, 818
Mox20I TGGCCA 1 cut(s) 543
MroI TCCGGA 1 cut(s) 418
MroNI GCCGGC 3 cut(s) 41, 158, 643
MscI TGGCCA 1 cut(s) 543
MseI TTAA 3 cut(s) 233, 285, 666
MslI CAYNNNNRTG 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 8 cut(s) 42, 159, 273, 419, 443, 513, 644, 760
MspR9I CCNGG 3 cut(s) 210, 761, 823
MvaI CCWGG 2 cut(s) 210, 823
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 557
NaeI GCCGGC 3 cut(s) 43, 160, 645
NarI GGCGCC 1 cut(s) 276
NciI CCSGG 1 cut(s) 761
NdeI CATATG 1 cut(s) 454
NdeII GATC 4 cut(s) 7, 249, 449, 789
NgoMIV GCCGGC 3 cut(s) 41, 158, 643
NlaIII CATG 3 cut(s) 250, 787, 820
NlaIV GGNNCC 3 cut(s) 40, 277, 373
NmuCI GTSAC 1 cut(s) 295
NruI TCGCGA 1 cut(s) 321
PagI TCATGA 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PdiI GCCGGC 3 cut(s) 43, 160, 645
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 2 cut(s) 759, 821
PinAI ACCGGT 1 cut(s) 442
PkrI GCNGC 2 cut(s) 47, 363
PleI GAGTC 1 cut(s) 666
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 208, 821
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 2 cut(s) 208, 821
PspN4I GGNNCC 3 cut(s) 40, 277, 373
PspPI GGNCC 3 cut(s) 599, 641, 692
RruI TCGCGA 1 cut(s) 321
RsaI GTAC 4 cut(s) 139, 350, 414, 441
RsaNI GTAC 4 cut(s) 138, 349, 413, 440
RseI CAYNNNNRTG 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 767
SaqAI TTAA 3 cut(s) 233, 285, 666
SatI GCNGC 2 cut(s) 46, 362
Sau3AI GATC 4 cut(s) 7, 249, 449, 789
Sau96I GGNCC 3 cut(s) 599, 641, 692
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 3 cut(s) 210, 761, 823
SfcI CTRYAG 1 cut(s) 561
SfoI GGCGCC 1 cut(s) 277
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 4 cut(s) 15, 120, 555, 730
SsiI CCGC 3 cut(s) 237, 291, 597
SspDI GGCGCC 1 cut(s) 275
SspI AATATT 1 cut(s) 711
StyD4I CCNGG 3 cut(s) 208, 759, 821
TaaI ACNGT 4 cut(s) 339, 412, 439, 842
TaiI ACGT 1 cut(s) 395
TaqI TCGA 4 cut(s) 170, 582, 617, 788
TasI AATT 4 cut(s) 15, 120, 555, 730
TatI WGTACW 1 cut(s) 348
Tru1I TTAA 3 cut(s) 233, 285, 666
Tru9I TTAA 3 cut(s) 233, 285, 666
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 45, 361
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 3 cut(s) 75, 635, 671
TspGWI ACGGA 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 692
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.