RLG00000031247

(E,E)-alpha-farnesene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002039
Physical Location & Seq
Forward (+)
3346457 .. 3348971
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031247

Sequence Viewer

Length: 1674 bp
ATGCACTTGCAAAACCCTCAAGCTGATGAGCGGCAGATACTGCAATGGCAGACTAAATCTGAACCCTCTTACTCTTTACATGATCAAAGACGATTTGCAAATTACAAGCCAAATATTTGGAAGTTTGAGTTCCTCGAATCCCTTAACAGCAAATTTGATGGAGAATGTTATCGAATTCAGATTCAGAAGCTAAGAGAAGATGTAAAGGAAATGTTTGTTGAAAGTAAGGAATCAGATTTAATAACCAAGTTGGAGCTGATTGATAGCATCCAAAAACTAGGCCTCAACAACCATTTTGAAAAGGAAATCAAGAAAGCCCTAGACACCATAGCATTTGTTGAACTCGAAAACAACAGCAATCCATACATTAGTTCAGCAGGTGATCTCTATGCTGCTGCACTACTCTTTAAGATTCTTAGGCAGCATGGTTATAAAGTTTCACAAGATACATACACAGATAAGAACACATTTGGGGATGTGAAAGGAATGCTTGAAATGTTGGAGGCCTCAAACTTAGCTTTAGAAGGTGAAAATATCCTAGAGGAGGCTAAAGCTTTCTTAATGGTCACTCTCAGAGATATCAATACTAGGTGTGATGATATAGACAATTGCATTTCCAAGCATTGGTTTAATGTGAAATGGCACATCAAATCCTACGAGAAAGACCATACTGATCAAGCCAACATGACCACTTTACTTGAGTTGGCTAAACTTAACTTCAACATGGTTCAAGCCACACTCCAAAAAGATCTAAGAGAAGTATCCATGTGGTGGAACAATCTGGCTCTCAATAAGAACTTGGACTTTGCAAGAGATAGACCAGTCGAATGTTTCATGTCTTCTGTGGGATTAGCTTTCGAGCCTGAGTACCAGTCCTTTACAAAATGGATACCTAAAGTCATCAATTTGATTCTCATCATCGATGACGTTTATGATGTTTATGGCTCATTGGAAGAGCTAAAACATTTCACCAATGCTGTTGAAAAGTGGGATATTAGGGAAACTGAGCAGCTTCCGGAGTGTAAGAAGATCTGTTTCCAAGTACTGTACAATACTACTTGTGAAATTGAATATGAAATTGAGGAGGGGAATGGTTGGAACCAAGTATTACCTCATTTGAGGAAAGTGTGGGGAGATTTCTGTAAAGCATTATTGGTGGACGCAGAGTGGTACAATAAGGCCTGCACACCGTCCTTTGAAGAGCACCTTAGCAATGGTTGCATTTCATCATCTGCTTCGCTGATTTTGACTCATGCCTTCTTCTCCAGAACACATGAGGAAGGGGCCAAAAACATTGCTGATTTTCTGCACAAGAATGAGGATCTTGTGTACAATATCTCTTTGATACTTCGACTCTTAAACGATCTAGGAACTTCCGCGGCTGAGCAAGAGAGACGTGATGCTGCTTCATTAATCCTATGTTACATGAGAGAAGCGAATGCTTCAGAAGAAATAGCTCGAAAGCACATCAAATGCATGATAGACTCAAATGCATGGAAGAAAATAAACAAAAAATGCTTCACTCAAATGCCCGAAATATCTTCATTCATCAACATTATGACAAACATAGCTCGGGTGGGGCATAGCCTTTATCAAGATGGAGATGGATTTGGTGATCAAAAGCACGGGACTCGGGGACAGATCCAATCTTTACTGGTTGAACCTCTCTCTTAA

Protein Analysis

558

Amino Acids

64.53

Weight (kDa)

5.31

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 39 - 195 6.8e-35 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 260 - 500 2.5e-78 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 299 - 496 2.6e-47 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AarI CACCTGC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 368
AccB7I CCANNNNNTGG 2 cut(s) 624, 771
AccBSI CCGCTC 1 cut(s) 31
AccII CGCG 1 cut(s) 1379
AccIII TCCGGA 1 cut(s) 1015
AciI CCGC 3 cut(s) 31, 1377, 1379
AclWI GGATC 2 cut(s) 1329, 1636
AcsI RAATTY 2 cut(s) 152, 174
AcuI CTGAAG 1 cut(s) 1428
AfaI GTAC 5 cut(s) 869, 1044, 1049, 1172, 1331
AfiI CCNNNNNNNGG 3 cut(s) 544, 624, 771
AjiI CACGTC 1 cut(s) 1397
Alw21I GWGCWC 1 cut(s) 1206
Alw26I GTCTC 1 cut(s) 1387
AlwI GGATC 2 cut(s) 1329, 1636
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 2 cut(s) 1572, 1632
Aor13HI TCCGGA 1 cut(s) 1015
AoxI GGCC 4 cut(s) 280, 504, 1179, 1284
ApeKI GCWGC 5 cut(s) 392, 395, 421, 1009, 1403
ApoI RAATTY 2 cut(s) 152, 174
AseI ATTAAT 1 cut(s) 1412
AspS9I GGNCC 1 cut(s) 1284
AsuHPI GGTGA 4 cut(s) 392, 539, 961, 1625
AvaI CYCGRG 2 cut(s) 1572, 1632
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 1206
BbvI GCAGC 5 cut(s) 379, 382, 433, 1021, 1390
BccI CCATC 3 cut(s) 152, 1592, 1598
BcgI CGANNNNNNTGC 2 cut(s) 1613, 1647
BciVI GTATCC 2 cut(s) 772, 882
BclI TGATCA 3 cut(s) 82, 673, 1615
BcoDI GTCTC 1 cut(s) 1387
BfaI CTAG 5 cut(s) 278, 320, 539, 588, 1367
BfuAI ACCTGC 1 cut(s) 368
BfuI GTATCC 2 cut(s) 772, 882
BglII AGATCT 2 cut(s) 748, 1029
BisI GCNGC 7 cut(s) 32, 393, 396, 422, 1010, 1380, 1404
BlpI GCTNAGC 1 cut(s) 1383
BlsI GCNGC 7 cut(s) 33, 394, 397, 423, 1011, 1381, 1405
BmcAI AGTACT 1 cut(s) 1044
BmeT110I CYCGRG 2 cut(s) 1572, 1632
BmgBI CACGTC 1 cut(s) 1397
BmgT120I GGNCC 1 cut(s) 1284
BmiI GGNNCC 2 cut(s) 1100, 1285
BmsI GCATC 2 cut(s) 276, 1390
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 1249
Bpu10I CCTNAGC 1 cut(s) 1208
Bpu1102I GCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 1 cut(s) 719
Bsa29I ATCGAT 1 cut(s) 921
BsaBI GATNNNNATC 1 cut(s) 914
BsaJI CCNNGG 1 cut(s) 1377
BsaWI WCCGGW 1 cut(s) 1015
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 624, 771
Bse1I ACTGG 3 cut(s) 821, 871, 1659
Bse3DI GCAATG 3 cut(s) 50, 1219, 1293
Bse8I GATNNNNATC 1 cut(s) 914
BseAI TCCGGA 1 cut(s) 1015
BseCI ATCGAT 1 cut(s) 921
BseDI CCNNGG 1 cut(s) 1377
BseGI GGATG 2 cut(s) 267, 481
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 3 cut(s) 544, 624, 771
BseMI GCAATG 3 cut(s) 50, 1219, 1293
BseMII CTCAG 4 cut(s) 586, 855, 996, 1374
BseNI ACTGG 3 cut(s) 821, 871, 1659
BseRI GAGGAG 2 cut(s) 557, 1097
BseXI GCAGC 5 cut(s) 379, 382, 433, 1021, 1390
BsgI GTGCAG 3 cut(s) 381, 1168, 1292
Bsh1236I CGCG 1 cut(s) 1379
BshFI GGCC 4 cut(s) 282, 506, 1181, 1286
BshVI ATCGAT 1 cut(s) 921
BsiHKAI GWGCWC 1 cut(s) 1206
BsiHKCI CYCGRG 2 cut(s) 1572, 1632
BsiSI CCGG 1 cut(s) 1016
BslFI GGGAC 2 cut(s) 1642, 1650
BslI CCNNNNNNNGG 3 cut(s) 544, 624, 771
BsmAI GTCTC 1 cut(s) 1387
BsmBI CGTCTC 1 cut(s) 1387
BsmFI GGGAC 2 cut(s) 1642, 1650
BsmI GAATGC 2 cut(s) 492, 1444
BsnI GGCC 4 cut(s) 282, 506, 1181, 1286
BsoBI CYCGRG 2 cut(s) 1572, 1632
Bsp1286I GDGCHC 1 cut(s) 1206
Bsp13I TCCGGA 1 cut(s) 1015
Bsp1407I TGTACA 2 cut(s) 1047, 1329
Bsp143I GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
Bsp1720I GCTNAGC 1 cut(s) 1383
BspACI CCGC 3 cut(s) 31, 1377, 1379
BspANI GGCC 4 cut(s) 282, 506, 1181, 1286
BspCNI CTCAG 4 cut(s) 585, 856, 997, 1375
BspDI ATCGAT 1 cut(s) 921
BspEI TCCGGA 1 cut(s) 1015
BspFNI CGCG 1 cut(s) 1379
BspLI GGNNCC 2 cut(s) 1100, 1285
BspMI ACCTGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 1329, 1636
BspQI GCTCTTC 2 cut(s) 948, 1194
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 50, 1219, 1293
BsrGI TGTACA 2 cut(s) 1047, 1329
BsrI ACTGG 3 cut(s) 821, 871, 1659
BssECI CCNNGG 1 cut(s) 1377
BssMI GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
Bst4CI ACNGT 2 cut(s) 1047, 1191
Bst6I CTCTTC 2 cut(s) 948, 1194
BstAPI GCANNNNNTGC 2 cut(s) 40, 1218
BstAUI TGTACA 2 cut(s) 1047, 1329
BstC8I GCNNGC 1 cut(s) 1183
BstDEI CTNAG 9 cut(s) 191, 416, 514, 572, 752, 864, 1005, 1208, 1383
BstDSI CCRYGG 1 cut(s) 1377
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 2 cut(s) 267, 481
BstFNI CGCG 1 cut(s) 1379
BstKTI GATC 9 cut(s) 85, 385, 676, 751, 1032, 1324, 1366, 1618, 1644
BstMAI GTCTC 1 cut(s) 1387
BstMBI GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
BstMWI GCNNNNNNNGC 2 cut(s) 40, 1218
BstUI CGCG 1 cut(s) 1379
BstV1I GCAGC 5 cut(s) 379, 382, 433, 1021, 1390
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 4 cut(s) 748, 1029, 1321, 1641
BstXI CCANNNNNNTGG 1 cut(s) 117
BstYI RGATCY 4 cut(s) 748, 1029, 1321, 1641
Bsu15I ATCGAT 1 cut(s) 921
BsuI GTATCC 2 cut(s) 772, 882
BsuRI GGCC 4 cut(s) 282, 506, 1181, 1286
BsuTUI ATCGAT 1 cut(s) 921
BtgI CCRYGG 1 cut(s) 1377
BtrI CACGTC 1 cut(s) 1397
BtsCI GGATG 2 cut(s) 267, 481
BveI ACCTGC 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 1183
CaiI CAGNNNCTG 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 1284
Cfr42I CCGCGG 1 cut(s) 1380
ClaI ATCGAT 1 cut(s) 921
CseI GACGC 1 cut(s) 1169
Csp6I GTAC 5 cut(s) 868, 1043, 1048, 1171, 1330
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviQI GTAC 5 cut(s) 868, 1043, 1048, 1171, 1330
DdeI CTNAG 9 cut(s) 191, 416, 514, 572, 752, 864, 1005, 1208, 1383
DpnI GATC 9 cut(s) 84, 384, 675, 750, 1031, 1323, 1365, 1617, 1643
DpnII GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
Eam1104I CTCTTC 2 cut(s) 948, 1194
EarI CTCTTC 2 cut(s) 948, 1194
Eco147I AGGCCT 3 cut(s) 282, 506, 1181
Eco32I GATATC 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 1428
Eco88I CYCGRG 2 cut(s) 1572, 1632
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoRI GAATTC 1 cut(s) 174
EcoRV GATATC 1 cut(s) 580
EcoT22I ATGCAT 2 cut(s) 1478, 1495
Esp3I CGTCTC 1 cut(s) 1387
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 1191, 1223
FaqI GGGAC 2 cut(s) 1642, 1650
FbaI TGATCA 3 cut(s) 82, 673, 1615
Fnu4HI GCNGC 7 cut(s) 32, 393, 396, 422, 1010, 1380, 1404
FokI GGATG 2 cut(s) 254, 488
Fsp4HI GCNGC 7 cut(s) 32, 393, 396, 422, 1010, 1380, 1404
FspBI CTAG 5 cut(s) 278, 320, 539, 588, 1367
GluI GCNGC 7 cut(s) 32, 393, 396, 422, 1010, 1380, 1404
GsuI CTGGAG 1 cut(s) 1249
HaeIII GGCC 4 cut(s) 282, 506, 1181, 1286
HapII CCGG 1 cut(s) 1016
HgaI GACGC 1 cut(s) 1169
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 9 cut(s) 137, 181, 230, 412, 910, 1249, 1353, 1484, 1630
HpaII CCGG 1 cut(s) 1016
HphI GGTGA 4 cut(s) 392, 539, 961, 1625
Hpy166II GTNNAC 2 cut(s) 1159, 1330
Hpy188I TCNGA 6 cut(s) 61, 180, 186, 235, 575, 1447
Hpy188III TCNNGA 4 cut(s) 310, 1016, 1266, 1595
Hpy8I GTNNAC 2 cut(s) 1159, 1330
HpyAV CCTTC 3 cut(s) 518, 1267, 1274
HpyCH4III ACNGT 2 cut(s) 1047, 1191
HpyCH4IV ACGT 2 cut(s) 927, 1396
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 1218
HpyF3I CTNAG 9 cut(s) 191, 416, 514, 572, 752, 864, 1005, 1208, 1383
HpySE526I ACGT 2 cut(s) 927, 1396
Kpn2I TCCGGA 1 cut(s) 1015
Ksp22I TGATCA 3 cut(s) 82, 673, 1615
KspI CCGCGG 1 cut(s) 1380
Kzo9I GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
LguI GCTCTTC 2 cut(s) 948, 1194
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 9 cut(s) 363, 767, 834, 876, 884, 1029, 1195, 1279, 1640
Lsp1109I GCAGC 5 cut(s) 379, 382, 433, 1021, 1390
LweI GCATC 2 cut(s) 276, 1390
MaeI CTAG 5 cut(s) 278, 320, 539, 588, 1367
MaeII ACGT 2 cut(s) 927, 1396
MaeIII GTNAC 2 cut(s) 565, 1421
MalI GATC 9 cut(s) 84, 384, 675, 750, 1031, 1323, 1365, 1617, 1643
MbiI CCGCTC 1 cut(s) 31
MboI GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
MboII GAAGA 9 cut(s) 209, 831, 965, 1039, 1211, 1252, 1460, 1510, 1533
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 4 cut(s) 748, 1029, 1321, 1641
MhlI GDGCHC 1 cut(s) 1206
MluCI AATT 7 cut(s) 100, 152, 174, 607, 904, 1065, 1077
MlyI GAGTC 4 cut(s) 1243, 1347, 1478, 1624
MmeI TCCRAC 3 cut(s) 231, 480, 1076
Mph1103I ATGCAT 2 cut(s) 1478, 1495
MroI TCCGGA 1 cut(s) 1015
MseI TTAA 9 cut(s) 144, 239, 408, 560, 630, 714, 1358, 1412, 1672
MslI CAYNNNNRTG 2 cut(s) 1314, 1526
MspA1I CMGCKG 1 cut(s) 1379
MspI CCGG 1 cut(s) 1016
MunI CAATTG 1 cut(s) 607
Mva1269I GAATGC 2 cut(s) 492, 1444
MvnI CGCG 1 cut(s) 1379
MwoI GCNNNNNNNGC 2 cut(s) 40, 1218
NdeII GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
NlaIV GGNNCC 2 cut(s) 1100, 1285
NmuCI GTSAC 1 cut(s) 565
NsiI ATGCAT 2 cut(s) 1478, 1495
PaqCI CACCTGC 1 cut(s) 368
PceI AGGCCT 3 cut(s) 282, 506, 1181
PciSI GCTCTTC 2 cut(s) 948, 1194
PctI GAATGC 2 cut(s) 492, 1444
PfeI GAWTC 5 cut(s) 137, 181, 230, 412, 910
PflMI CCANNNNNTGG 2 cut(s) 624, 771
PkrI GCNGC 7 cut(s) 33, 394, 397, 423, 1011, 1381, 1405
PleI GAGTC 4 cut(s) 1243, 1347, 1478, 1624
PpsI GAGTC 4 cut(s) 1243, 1347, 1478, 1624
PshBI ATTAAT 1 cut(s) 1412
PsiI TTATAA 1 cut(s) 432
PspN4I GGNNCC 2 cut(s) 1100, 1285
PspPI GGNCC 1 cut(s) 1284
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 4 cut(s) 748, 1029, 1321, 1641
RsaI GTAC 5 cut(s) 869, 1044, 1049, 1172, 1331
RsaNI GTAC 5 cut(s) 868, 1043, 1048, 1171, 1330
RseI CAYNNNNRTG 2 cut(s) 1314, 1526
SacII CCGCGG 1 cut(s) 1380
SapI GCTCTTC 2 cut(s) 948, 1194
SaqAI TTAA 9 cut(s) 144, 239, 408, 560, 630, 714, 1358, 1412, 1672
SatI GCNGC 7 cut(s) 32, 393, 396, 422, 1010, 1380, 1404
Sau3AI GATC 9 cut(s) 82, 382, 673, 748, 1029, 1321, 1363, 1615, 1641
Sau96I GGNCC 1 cut(s) 1284
ScaI AGTACT 1 cut(s) 1044
SchI GAGTC 4 cut(s) 1243, 1347, 1478, 1624
SduI GDGCHC 1 cut(s) 1206
SfaNI GCATC 2 cut(s) 276, 1390
Sfr303I CCGCGG 1 cut(s) 1380
SgrBI CCGCGG 1 cut(s) 1380
SmiMI CAYNNNNRTG 2 cut(s) 1314, 1526
SmlI CTYRAG 2 cut(s) 18, 698
SmoI CTYRAG 2 cut(s) 18, 698
Sse9I AATT 7 cut(s) 100, 152, 174, 607, 904, 1065, 1077
SseBI AGGCCT 3 cut(s) 282, 506, 1181
SsiI CCGC 3 cut(s) 31, 1377, 1379
SspI AATATT 1 cut(s) 115
SspMI CTAG 5 cut(s) 278, 320, 539, 588, 1367
StuI AGGCCT 3 cut(s) 282, 506, 1181
TaaI ACNGT 2 cut(s) 1047, 1191
TaiI ACGT 2 cut(s) 930, 1399
TaqI TCGA 8 cut(s) 135, 172, 345, 825, 858, 921, 1351, 1459
TasI AATT 7 cut(s) 100, 152, 174, 607, 904, 1065, 1077
TatI WGTACW 3 cut(s) 1042, 1047, 1329
TauI GCSGC 2 cut(s) 34, 1382
TfiI GAWTC 5 cut(s) 137, 181, 230, 412, 910
Tru1I TTAA 9 cut(s) 144, 239, 408, 560, 630, 714, 1358, 1412, 1672
Tru9I TTAA 9 cut(s) 144, 239, 408, 560, 630, 714, 1358, 1412, 1672
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 5 cut(s) 392, 395, 421, 1009, 1403
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 6 cut(s) 823, 1089, 1215, 1398, 1533, 1537
Van91I CCANNNNNTGG 2 cut(s) 624, 771
VspI ATTAAT 1 cut(s) 1412
XagI CCTNNNNNAGG 1 cut(s) 542
XapI RAATTY 2 cut(s) 152, 174
XspI CTAG 5 cut(s) 278, 320, 539, 588, 1367
ZrmI AGTACT 1 cut(s) 1044
Zsp2I ATGCAT 2 cut(s) 1478, 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.