MD11G1213300.v1.1

Eukaryotic translation initiation factor eIF2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002681
Physical Location & Seq
Reverse (-)
31197943 .. 31198263
321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1213300.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGTCTCCGCCTTCGACGACAAGACCCTCCGCCTCTGGGACGTCGCCACCGGCCAAACCATCAAGACCCTCCATGGCCACACCAACTACGTCTTCTGCGCCAACTTCAACCCTCAGTCCAATATGATCGTCTCCGGTTCCTTCGACGAAACGGTGCGTATTTGGGATGTCAAGACCGGGAAATGCTTGAAGGTTTTGCCGGCTCACTCCGACCCTGTCACCGCCGTTGATTTCAATCGGGATGGCTCGGTTATCGTTTCGAGCTGCTACGATGGGTTGTGCAGGACCTGGGATGCCTCCACCGCCCTCTGCACCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0001085 GO:0001501 GO:0001654 GO:0002682 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005671 GO:0005737 GO:0005815 GO:0005856 GO:0005929 GO:0006109 GO:0006111 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007482 GO:0007568 GO:0008134 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008340 GO:0008757 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010259 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010675 GO:0010676 GO:0010906 GO:0010907 GO:0015630 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022008 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030162 GO:0030182 GO:0031175 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032501 GO:0032502 GO:0032991 GO:0034708 GO:0034968 GO:0035064 GO:0035097 GO:0035216 GO:0035295 GO:0035947 GO:0035948 GO:0036064 GO:0036211 GO:0042054 GO:0042176 GO:0042393 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043255 GO:0043412 GO:0043414 GO:0043543 GO:0043687 GO:0043966 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044446 GO:0044451 GO:0044463 GO:0044464 GO:0044545 GO:0044665 GO:0044666 GO:0044782 GO:0045595 GO:0045637 GO:0045652 GO:0045722 GO:0045732 GO:0045862 GO:0045893 GO:0045913 GO:0045935 GO:0045944 GO:0048188 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051173 GO:0051239 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0060255 GO:0060271 GO:0060290 GO:0060429 GO:0061136 GO:0062012 GO:0062013 GO:0065007 GO:0070013 GO:0070925 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:0140030 GO:0140034 GO:0140096 GO:1900402 GO:1901564 GO:1901800 GO:1902493 GO:1902494 GO:1902562 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1903706 GO:1990234 GO:2000026 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

107

Amino Acids

11.72

Weight (kDa)

6.23

Isoelectric Point (pI)

22.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 1 - 105 3.7e-52 WDR5 beta-propeller domain
WDR55 PF24796 1 - 100 3.9e-10 WDR55
Beta-prop_SCAP PF24017 2 - 103 8.2e-09 SCAP Beta-propeller
Beta-prop_WDR41 PF25178 2 - 74 3.6e-09 WDR41
Beta-prop_THOC3 PF25174 2 - 102 1.4e-23 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 2 - 66 6.2e-13 WDHD1 first WD40 domain
EIF3I PF24805 2 - 86 1.2e-14 EIF3I
WD40_Gbeta PF25391 2 - 99 2.9e-16 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 3 - 104 4.9e-20 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 3 - 97 1.9e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 4 - 105 2.6e-27 WDR3 first beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 4 - 102 1.3e-12 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 4 - 63 3.9e-10 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 4 - 101 1.2e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 4 - 100 2.1e-16 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 6 - 101 1.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 6 - 98 5.5e-09 WDR36/Utp21 first beta-propeller
WDR90_beta-prop_4th PF23342 6 - 95 5.4e-06 WDR90, 4th beta-propeller
Beta-prop_WDR75_1st PF23869 9 - 58 7.4e-06 WD repeat-containing protein 75 first beta-propeller
WD40_MABP1-WDR62_1st PF24780 9 - 88 2.1e-08 MABP1/WDR62 first WD40 domain
NBCH_WD40 PF20426 10 - 102 6.3e-07 Neurobeachin beta propeller domain
Beta-prop_IFT122_1st PF23381 10 - 89 4.7e-07 IFT122 first beta-propeller
WD40 PF00400 18 - 56 1.7e-11 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 20 - 103 9.1e-14 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 28 - 104 2.9e-07 Aladin seven-bladed propeller
Beta-prop_TEP1_2nd PF25047 39 - 100 4.5e-08 TEP-1 second beta-propeller
WD40 PF00400 60 - 98 1.1e-10 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
AciI CCGC 4 cut(s) 9, 31, 222, 303
AcoI YGGCCR 2 cut(s) 52, 76
AcyI GRCGYC 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 3 cut(s) 109, 190, 235
AjnI CCWGG 2 cut(s) 287, 314
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 1 cut(s) 264
AluI AGCT 1 cut(s) 264
Alw26I GTCTC 2 cut(s) 10, 136
AlwNI CAGNNNCTG 1 cut(s) 288
AoxI GGCC 2 cut(s) 52, 76
ApeKI GCWGC 1 cut(s) 264
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 1 cut(s) 285
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 285
BalI TGGCCA 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 251
BccI CCATC 3 cut(s) 68, 236, 266
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 2 cut(s) 289, 316
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 10, 136
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme1390I CCNGG 3 cut(s) 178, 289, 316
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 3 cut(s) 178, 289, 316
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 85
BpuMI CCSGG 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 73, 288
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 2 cut(s) 50, 199
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 2 cut(s) 289, 316
BseDI CCNNGG 2 cut(s) 73, 288
BseGI GGATG 3 cut(s) 172, 247, 298
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 251
BsgI GTGCAG 2 cut(s) 295, 301
BshFI GGCC 2 cut(s) 54, 78
BsiSI CCGG 4 cut(s) 51, 135, 177, 200
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 10, 136
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 2 cut(s) 54, 78
Bso31I GGTCTC 1 cut(s) 10
Bsp143I GATC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 4 cut(s) 9, 31, 222, 303
BspANI GGCC 2 cut(s) 54, 78
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 2 cut(s) 50, 199
BssAI RCCGGY 2 cut(s) 50, 199
BssECI CCNNGG 2 cut(s) 73, 288
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 289, 316
Bst4CI ACNGT 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 3 cut(s) 172, 247, 298
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 10, 136
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 2 cut(s) 289, 316
BstSCI CCNGG 3 cut(s) 176, 287, 314
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 54, 78
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 3 cut(s) 172, 247, 298
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 1 cut(s) 288
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 2 cut(s) 50, 199
Cfr13I GGNCC 1 cut(s) 285
CsiI ACCWGGT 1 cut(s) 314
CviAII CATG 1 cut(s) 74
CviJI RGCY 5 cut(s) 54, 78, 203, 246, 264
CviKI_1 RGCY 5 cut(s) 54, 78, 203, 246, 264
DdeI CTNAG 1 cut(s) 114
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EaeI YGGCCR 2 cut(s) 52, 76
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 287, 314
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 1 cut(s) 77
FaiI YATR 2 cut(s) 75, 125
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 73
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 3 cut(s) 179, 254, 305
Fsp4HI GCNGC 1 cut(s) 265
GlaI GCGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 265
HaeIII GGCC 2 cut(s) 54, 78
HapII CCGG 4 cut(s) 51, 135, 177, 200
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 1 cut(s) 77
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HpaII CCGG 4 cut(s) 51, 135, 177, 200
HphI GGTGA 1 cut(s) 211
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 3 cut(s) 64, 172, 239
Hpy99I CGWCG 3 cut(s) 20, 47, 149
HpyAV CCTTC 3 cut(s) 22, 151, 184
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 42, 90
HpyCH4V TGCA 2 cut(s) 282, 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 2 cut(s) 42, 90
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 1 cut(s) 77
HspAI GCGC 1 cut(s) 99
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 1 cut(s) 126
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 1 cut(s) 283
MabI ACCWGGT 1 cut(s) 314
MaeII ACGT 2 cut(s) 42, 90
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 78
MluNI TGGCCA 1 cut(s) 78
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 6 cut(s) 38, 44, 80, 123, 307, 317
Mox20I TGGCCA 1 cut(s) 78
MroNI GCCGGC 1 cut(s) 199
MscI TGGCCA 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 78
MspI CCGG 4 cut(s) 51, 135, 177, 200
MspR9I CCNGG 3 cut(s) 178, 289, 316
MvaI CCWGG 2 cut(s) 289, 316
MwoI GCNNNNNNNGC 1 cut(s) 302
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 178
NcoI CCATGG 1 cut(s) 73
NdeII GATC 1 cut(s) 126
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 1 cut(s) 77
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 217
PdiI GCCGGC 1 cut(s) 201
PflFI GACNNNGTC 1 cut(s) 215
PkrI GCNGC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 287, 314
PspGI CCWGG 2 cut(s) 287, 314
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 1 cut(s) 285
PspPPI RGGWCCY 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 288
PsyI GACNNNGTC 1 cut(s) 215
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 285
ScrFI CCNGG 3 cut(s) 178, 289, 316
SetI ASST 6 cut(s) 45, 93, 195, 266, 290, 320
SexAI ACCWGGT 1 cut(s) 314
SfaNI GCATC 1 cut(s) 283
SinI GGWCC 1 cut(s) 285
SsiI CCGC 4 cut(s) 9, 31, 222, 303
StyD4I CCNGG 3 cut(s) 176, 287, 314
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 45, 93
TaqI TCGA 3 cut(s) 15, 144, 260
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 264
Tsp45I GTSAC 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 285
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.