pycom01g07290

WD repeat domain 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002681
Physical Location & Seq
Forward (+)
8107551 .. 8109299
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07290.1

Sequence Viewer

Length: 798 bp
ATGACTCCTCCACTCCCACCATCTCCGCCGCCCAGACCTTCGAAGGCCACTCCCAGGGCGTCTCCGATCTCGCCTTCTCCGCCGACTCTCGCTTCATGGTCTCCGCCTCCGACGACAAGACCCTCCGCCTCTGGGACGTCGCCACCGGCCAAACCATCAAGACCCTCCATGGCCACACCAACTACGTCTTCTGCGCCAACTTCAACCCTCAGTCCAATATCGATCGTCTCCGGTTCCTTCGACGAAACGGTCCGTATTTGGGATGTCAAGACCGGGACATGCTTGAAGGTTTTGCCGGCTCACTCCGACCCTGTCACCGCCGTTGATTTCAATCGGGATGGCTCGGTTATCGTTTCGAGCAGCTACGATGGGTTGTGCAGGACCTGGGATGCCTCCACTGGCCACTGCACCAAGACATTGATTGATGATGAGAATCCTCCGGTTTCGTTTGTTAAGTTTGCGCCTAATGGAAAGTACATTCTTGTTGGCACTTTGGATGACACTCTGAGGCTCTGGAACTTTTCAACTGGGAAATGTCTAAAGACTTACACCGGTCACAAAAGCTCGAGATTCTGCATTTCTTCCGCATTTTCTGTGACAAATGGGATATACATTGTAAGTGGTTCTGAAGATAATTGTTTGTACTTGTGGGAATTGCAAAGCAGGAAGGTAGTTCAGAAATTGGAAGGTCACACTGACACTGTTATATCAGTATCATGTCACCCAACTAAGAACATAATTGCTTCTGGTGCACTTGGGAATGACAAGACGGTGAAGATCTGGACTCAGGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0001085 GO:0001501 GO:0001654 GO:0002682 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005671 GO:0005737 GO:0005815 GO:0005856 GO:0005929 GO:0006109 GO:0006111 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007482 GO:0007568 GO:0008134 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008340 GO:0008757 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010259 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010675 GO:0010676 GO:0010906 GO:0010907 GO:0015630 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022008 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030162 GO:0030182 GO:0031175 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032501 GO:0032502 GO:0032991 GO:0034708 GO:0034968 GO:0035064 GO:0035097 GO:0035216 GO:0035295 GO:0035947 GO:0035948 GO:0036064 GO:0036211 GO:0042054 GO:0042176 GO:0042393 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043255 GO:0043412 GO:0043414 GO:0043543 GO:0043687 GO:0043966 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044446 GO:0044451 GO:0044463 GO:0044464 GO:0044545 GO:0044665 GO:0044666 GO:0044782 GO:0045595 GO:0045637 GO:0045652 GO:0045722 GO:0045732 GO:0045862 GO:0045893 GO:0045913 GO:0045935 GO:0045944 GO:0048188 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048736 GO:0048737 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051173 GO:0051239 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0060255 GO:0060271 GO:0060290 GO:0060429 GO:0061136 GO:0062012 GO:0062013 GO:0065007 GO:0070013 GO:0070925 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:0140030 GO:0140034 GO:0140096 GO:1900402 GO:1901564 GO:1901800 GO:1902493 GO:1902494 GO:1902562 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1903706 GO:1990234 GO:2000026 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

266

Amino Acids

28.33

Weight (kDa)

8.82

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR90_beta-prop_4th PF23342 73 - 152 7.6e-06 WDR90, 4th beta-propeller
WD40_CDC20-Fz PF24807 73 - 133 1.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 74 - 143 7.2e-18 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 74 - 139 9.2e-08 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 74 - 229 7e-28 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 75 - 262 8.2e-93 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 75 - 173 4.3e-14 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 75 - 229 2.8e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 75 - 137 3.3e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 75 - 140 1.3e-11 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 75 - 140 1.8e-13 THOC3 beta-propeller domain
Beta-prop_EML PF23409 76 - 165 3.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 81 - 159 5.8e-08 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 92 - 130 3.9e-10 WD domain, G-beta repeat
Beta-prop_Aladin PF25460 97 - 231 8.4e-10 Aladin seven-bladed propeller
EIF3I PF24805 98 - 192 4e-09 EIF3I
WD40_Gbeta PF25391 98 - 221 6.9e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 123 - 262 1.7e-09 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 128 - 263 1.2e-20 CDC20/Fizzy WD40 domain
Beta-prop_NWD2_C PF23586 141 - 253 6.4e-06 NWD2 C-terminal beta-propeller
Beta-prop_TEP1_2nd PF25047 142 - 263 2.5e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 144 - 262 6e-21 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 145 - 189 8.9e-06 WDHD1 first WD40 domain
EIF3I PF24805 147 - 228 2.1e-06 EIF3I
Beta-prop_SCAP PF24017 147 - 262 6.7e-07 SCAP Beta-propeller
Beta-prop_THOC3 PF25174 148 - 249 1.3e-19 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 150 - 219 1.9e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 150 - 249 2.9e-08 WDR55
Beta-prop_WDR3_2nd PF25172 151 - 262 4.2e-11 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 154 - 248 6.5e-07 MABP1/WDR62 second WD40 domain
Beta-prop_EML PF23409 168 - 250 1.9e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 177 - 217 3.7e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 201 - 262 3.1e-11 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 201 - 264 3.2e-06 WDHD1 first WD40 domain
WD40 PF00400 223 - 261 4e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 140
AciI CCGC 7 cut(s) 26, 29, 80, 104, 126, 318, 585
AcoI YGGCCR 3 cut(s) 147, 171, 400
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 2 cut(s) 59, 137
AfaI GTAC 2 cut(s) 476, 644
AfiI CCNNNNNNNGG 3 cut(s) 54, 132, 259
AgeI ACCGGT 1 cut(s) 551
AgsI TTSAA 4 cut(s) 204, 286, 331, 525
AjnI CCWGG 2 cut(s) 53, 383
AjuI GAANNNNNNNTTGG 2 cut(s) 172, 204
AluBI AGCT 2 cut(s) 363, 564
AluI AGCT 2 cut(s) 363, 564
Alw21I GWGCWC 1 cut(s) 754
Alw26I GTCTC 3 cut(s) 66, 105, 232
Alw44I GTGCAC 1 cut(s) 750
AlwNI CAGNNNCTG 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 4 cut(s) 45, 147, 171, 400
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 1 cut(s) 360
AsiGI ACCGGT 1 cut(s) 551
AspLEI GCGC 2 cut(s) 196, 463
AspS9I GGNCC 2 cut(s) 250, 381
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 3 cut(s) 307, 713, 784
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 2 cut(s) 250, 381
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 2 cut(s) 173, 402
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 1 cut(s) 372
BccI CCATC 4 cut(s) 28, 163, 332, 362
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 2 cut(s) 55, 385
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 3 cut(s) 66, 105, 232
BglII AGATCT 1 cut(s) 777
BisI GCNGC 2 cut(s) 29, 361
BlsI GCNGC 2 cut(s) 30, 362
Bme1390I CCNGG 3 cut(s) 55, 274, 385
Bme18I GGWCC 2 cut(s) 250, 381
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 250, 381
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 3 cut(s) 55, 274, 385
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 221
BsaBI GATNNNNATC 2 cut(s) 330, 432
BsaHI GRCGYC 2 cut(s) 59, 137
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 4 cut(s) 53, 54, 168, 384
BsaWI WCCGGW 3 cut(s) 230, 439, 551
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 132, 259
Bse118I RCCGGY 3 cut(s) 145, 295, 551
Bse1I ACTGG 2 cut(s) 403, 532
Bse8I GATNNNNATC 2 cut(s) 330, 432
BseBI CCWGG 2 cut(s) 55, 385
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 4 cut(s) 53, 54, 168, 384
BseGI GGATG 4 cut(s) 268, 343, 394, 502
BseJI GATNNNNATC 2 cut(s) 330, 432
BseLI CCNNNNNNNGG 3 cut(s) 54, 132, 259
BseMII CTCAG 2 cut(s) 223, 497
BseNI ACTGG 2 cut(s) 403, 532
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 1 cut(s) 372
BsgI GTGCAG 2 cut(s) 391, 397
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 4 cut(s) 47, 149, 173, 402
BshTI ACCGGT 1 cut(s) 551
BshVI ATCGAT 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 6 cut(s) 146, 231, 273, 296, 440, 552
BslFI GGGAC 2 cut(s) 148, 289
BslI CCNNNNNNNGG 3 cut(s) 54, 132, 259
BsmAI GTCTC 3 cut(s) 66, 105, 232
BsmBI CGTCTC 2 cut(s) 66, 232
BsmFI GGGAC 2 cut(s) 148, 289
BsnI GGCC 4 cut(s) 47, 149, 173, 402
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 565
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 3 cut(s) 66, 222, 777
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 7 cut(s) 26, 29, 80, 104, 126, 318, 585
BspANI GGCC 4 cut(s) 47, 149, 173, 402
BspCNI CTCAG 2 cut(s) 222, 498
BspDI ATCGAT 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 105
BsrFI RCCGGY 3 cut(s) 145, 295, 551
BsrI ACTGG 2 cut(s) 403, 532
BssAI RCCGGY 3 cut(s) 145, 295, 551
BssECI CCNNGG 4 cut(s) 53, 54, 168, 384
BssMI GATC 3 cut(s) 66, 222, 777
BssNI GRCGYC 2 cut(s) 59, 137
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 55, 385
Bst4CI ACNGT 3 cut(s) 250, 703, 772
BstACI GRCGYC 2 cut(s) 59, 137
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 4 cut(s) 209, 506, 729, 786
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 4 cut(s) 268, 343, 394, 502
BstHHI GCGC 2 cut(s) 196, 463
BstKTI GATC 3 cut(s) 69, 225, 780
BstMAI GTCTC 3 cut(s) 66, 105, 232
BstMBI GATC 3 cut(s) 66, 222, 777
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 79, 749
BstNI CCWGG 2 cut(s) 55, 385
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 3 cut(s) 53, 272, 383
BstSLI GKGCMC 1 cut(s) 754
BstV1I GCAGC 1 cut(s) 372
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 777
BstYI RGATCY 1 cut(s) 777
Bsu15I ATCGAT 1 cut(s) 221
BsuRI GGCC 4 cut(s) 47, 149, 173, 402
BsuTUI ATCGAT 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 4 cut(s) 268, 343, 394, 502
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 4 cut(s) 396, 403, 693, 699
Cac8I GCNNGC 1 cut(s) 297
CaiI CAGNNNCTG 1 cut(s) 384
CfoI GCGC 2 cut(s) 196, 463
Cfr10I RCCGGY 3 cut(s) 145, 295, 551
Cfr13I GGNCC 2 cut(s) 250, 381
ClaI ATCGAT 1 cut(s) 221
CpoI CGGWCCG 1 cut(s) 250
CseI GACGC 1 cut(s) 48
Csp6I GTAC 2 cut(s) 475, 643
CspAI ACCGGT 1 cut(s) 551
CspI CGGWCCG 1 cut(s) 250
CviAII CATG 4 cut(s) 96, 169, 279, 717
CviJI RGCY 9 cut(s) 47, 149, 173, 299, 342, 363, 402, 511, 564
CviKI_1 RGCY 9 cut(s) 47, 149, 173, 299, 342, 363, 402, 511, 564
CviQI GTAC 2 cut(s) 475, 643
DdeI CTNAG 4 cut(s) 209, 506, 729, 786
DpnI GATC 3 cut(s) 68, 224, 779
DpnII GATC 3 cut(s) 66, 222, 777
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 3 cut(s) 147, 171, 400
EciI GGCGGA 4 cut(s) 15, 69, 93, 115
Eco130I CCWWGG 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 250, 381
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 53, 383
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
Esp3I CGTCTC 2 cut(s) 66, 232
FaeI CATG 4 cut(s) 99, 172, 282, 720
FaiI YATR 7 cut(s) 97, 170, 280, 610, 707, 718, 737
FaqI GGGAC 2 cut(s) 148, 289
FatI CATG 4 cut(s) 95, 168, 278, 716
Fnu4HI GCNGC 2 cut(s) 29, 361
FokI GGATG 4 cut(s) 275, 350, 401, 509
Fsp4HI GCNGC 2 cut(s) 29, 361
GlaI GCGC 2 cut(s) 195, 462
GluI GCNGC 2 cut(s) 29, 361
HaeIII GGCC 4 cut(s) 47, 149, 173, 402
HapII CCGG 6 cut(s) 146, 231, 273, 296, 440, 552
HgaI GACGC 1 cut(s) 48
HhaI GCGC 2 cut(s) 196, 463
Hin1I GRCGYC 2 cut(s) 59, 137
Hin1II CATG 4 cut(s) 99, 172, 282, 720
Hin6I GCGC 2 cut(s) 194, 461
HinP1I GCGC 2 cut(s) 194, 461
HinfI GANTC 5 cut(s) 4, 85, 433, 570, 784
HpaII CCGG 6 cut(s) 146, 231, 273, 296, 440, 552
HphI GGTGA 3 cut(s) 307, 713, 784
Hpy166II GTNNAC 1 cut(s) 752
Hpy188I TCNGA 6 cut(s) 66, 111, 307, 507, 628, 678
Hpy188III TCNNGA 7 cut(s) 159, 268, 335, 514, 567, 781, 788
Hpy8I GTNNAC 1 cut(s) 752
Hpy99I CGWCG 3 cut(s) 115, 142, 245
HpyAV CCTTC 7 cut(s) 37, 48, 84, 247, 280, 661, 680
HpyCH4III ACNGT 3 cut(s) 250, 703, 772
HpyCH4IV ACGT 2 cut(s) 137, 185
HpyCH4V TGCA 5 cut(s) 378, 408, 576, 658, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 749
HpyF3I CTNAG 4 cut(s) 209, 506, 729, 786
HpySE526I ACGT 2 cut(s) 137, 185
Hsp92I GRCGYC 2 cut(s) 59, 137
Hsp92II CATG 4 cut(s) 99, 172, 282, 720
HspAI GCGC 2 cut(s) 194, 461
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 66, 222, 777
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 1 cut(s) 379
MaeII ACGT 2 cut(s) 137, 185
MaeIII GTNAC 5 cut(s) 313, 554, 595, 689, 719
MalI GATC 3 cut(s) 68, 224, 779
MboI GATC 3 cut(s) 66, 222, 777
MboII GAAGA 4 cut(s) 180, 573, 641, 787
MflI RGATCY 1 cut(s) 777
MhlI GDGCHC 1 cut(s) 754
MlsI TGGCCA 2 cut(s) 173, 402
MluCI AATT 4 cut(s) 634, 653, 680, 738
MluNI TGGCCA 2 cut(s) 173, 402
MlyI GAGTC 2 cut(s) 79, 778
MmeI TCCRAC 2 cut(s) 134, 330
MnlI CCTC 9 cut(s) 18, 117, 133, 139, 175, 218, 403, 447, 501
Mox20I TGGCCA 2 cut(s) 173, 402
MroNI GCCGGC 1 cut(s) 295
MscI TGGCCA 2 cut(s) 173, 402
MseI TTAA 1 cut(s) 453
Msp20I TGGCCA 2 cut(s) 173, 402
MspI CCGG 6 cut(s) 146, 231, 273, 296, 440, 552
MspR9I CCNGG 3 cut(s) 55, 274, 385
MvaI CCWGG 2 cut(s) 55, 385
MwoI GCNNNNNNNGC 2 cut(s) 79, 749
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 66, 222, 777
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 4 cut(s) 99, 172, 282, 720
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 5 cut(s) 313, 554, 595, 689, 719
NspI RCATGY 1 cut(s) 282
NspV TTCGAA 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 565
PasI CCCWGGG 1 cut(s) 54
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 433, 570
PflFI GACNNNGTC 1 cut(s) 311
PinAI ACCGGT 1 cut(s) 551
PkrI GCNGC 2 cut(s) 30, 362
Ple19I CGATCG 1 cut(s) 225
PleI GAGTC 2 cut(s) 79, 778
PpsI GAGTC 2 cut(s) 79, 778
PpuMI RGGWCCY 1 cut(s) 381
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 53, 383
PspGI CCWGG 2 cut(s) 53, 383
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 2 cut(s) 250, 381
PspPPI RGGWCCY 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 777
PsyI GACNNNGTC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 225
RsaI GTAC 2 cut(s) 476, 644
RsaNI GTAC 2 cut(s) 475, 643
Rsr2I CGGWCCG 1 cut(s) 250
RsrII CGGWCCG 1 cut(s) 250
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 29, 361
Sau3AI GATC 3 cut(s) 66, 222, 777
Sau96I GGNCC 2 cut(s) 250, 381
SchI GAGTC 2 cut(s) 79, 778
ScrFI CCNGG 3 cut(s) 55, 274, 385
SduI GDGCHC 1 cut(s) 754
SetI ASST 9 cut(s) 40, 140, 188, 291, 365, 386, 566, 672, 691
SfaNI GCATC 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 41
SinI GGWCC 2 cut(s) 250, 381
SlaI CTCGAG 1 cut(s) 565
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 4 cut(s) 634, 653, 680, 738
SsiI CCGC 7 cut(s) 26, 29, 80, 104, 126, 318, 585
StyD4I CCNGG 3 cut(s) 53, 272, 383
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 3 cut(s) 250, 703, 772
TaiI ACGT 2 cut(s) 140, 188
TaqI TCGA 5 cut(s) 41, 221, 240, 356, 566
TasI AATT 4 cut(s) 634, 653, 680, 738
TatI WGTACW 2 cut(s) 474, 642
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 2 cut(s) 433, 570
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 4 cut(s) 403, 410, 700, 706
TseFI GTSAC 5 cut(s) 313, 554, 595, 689, 719
TseI GCWGC 1 cut(s) 360
Tsp45I GTSAC 5 cut(s) 313, 554, 595, 689, 719
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 4 cut(s) 403, 410, 700, 706
Tth111I GACNNNGTC 1 cut(s) 311
VneI GTGCAC 1 cut(s) 750
VpaK11BI GGWCC 2 cut(s) 250, 381
XceI RCATGY 1 cut(s) 282
XhoI CTCGAG 1 cut(s) 565
ZraI GACGTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.