Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002681

Physical Location & Seq

Genomic Coordinates Forward (+)

27032653 .. 27034547

Gene Structure 1895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g25220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGTCGAAGGAACCATCCACGACCTTCAATCCCTACACCCTCTGCGAAACCTTAACGGGCCACAAGAGCGCCATCTCCGGTGTCAAATTCTCCTCCGACGGCCGCCTCCTCGGTTCCGCTTCCGCGGACAAGACCATCCGCACCTACTCCCTTTCCTTCCCCGATGACTCCTCCACTCCCACCATCTCCGCCGCCCAGACCTTCGAAGGCCACTCCCAGGGCGTCTCCGATCTCGCCTTCTCCGCCGACTCTCGCTTCATGGTCTCCGCCTCCGACGACAAGACCCTCCGCCTCTGGGACGTCGCCACCGGCCAAACCATCAAGGCCCTCCATGGCCACACCAACTACGTCTTCTGCGCCAACTTCAACCCTCAGTCCAATATGATCGTCTCCGGTTCCTTCGACGAAACGGTGCGTATTTGGGATGTCAAGACCGGGAAATGCTTGAAGGTTTTGCCGGCTCACTCCGACCCTGTCACCGCCGTTGATTTCAATCGGGATGGCTCGGTTATCGTTTCGAGCAGCTACGATGGGTTGTGCAGGACCTGGGATGCCTCCACTGGCCACTGCACCAAGACATTGATTGACGATGAGAATCCTCCGGTTTCGTTTGTTAAATTTGCGCCTAATGGAAAGTACATTCTTGTTGGCACTTTGGATGACACTCTGAGGCTCTGGAACTTTTCAACTGGGAAATGTCTAAAGACTTACACCGGTCACAAGAGCTCAAGATTCTGCATTTCTTCCGCATTTTCTGTGACAAATGGGATATACATTGTAAGTGGTTCTGAAGATAATTGTTTGTACTTGTGGGAATTGCAAAGCAGGAAGGTAGTTCAGAAATTGGAAGGTCACACTGACACTGTTATATCAGTATCATGTCACCCAACAAAGAACATAATTGCTTCTGGTGCACTTGGGAATGACAAGACGGTGAAGATCTGGACTCAGGAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000123 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005815 GO:0005856 GO:0005929 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008213 GO:0009791 GO:0009987 GO:0010228 GO:0015630 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0022008 GO:0022414 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030182 GO:0031175 GO:0031248 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034708 GO:0034968 GO:0035097 GO:0036064 GO:0036211 GO:0042393 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044446 GO:0044451 GO:0044463 GO:0044464 GO:0044665 GO:0044782 GO:0048188 GO:0048468 GO:0048608 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0051276 GO:0051568 GO:0060271 GO:0061458 GO:0070013 GO:0070925 GO:0071339 GO:0071704 GO:0071840 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901564 GO:1902493 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

320

Amino Acids

34.73

Weight (kDa)

6.49

Isoelectric Point (pI)

27.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	11 - 183	5.7e-19	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	13 - 190	6.3e-22	TEP-1 second beta-propeller
WD40	PF00400	15 - 49	2.5e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	15 - 110	1.2e-14	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	16 - 110	3.3e-15	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	16 - 148	7.6e-19	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5	PF25175	17 - 316	9.7e-139	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 109	6.8e-13	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	18 - 109	8.6e-11	EIF3I
Beta-prop_THOC3	PF25174	21 - 303	1.1e-62	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	59 - 148	7.7e-06	Aladin seven-bladed propeller
Beta-prop_WDR41	PF25178	61 - 152	1e-10	WDR41
WD40_CDC20-Fz	PF24807	62 - 187	5e-18	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	64 - 108	3.1e-09	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	64 - 315	1.5e-25	WDR55
Beta-prop_WDR3_1st	PF25173	64 - 197	5e-34	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	65 - 275	1.7e-26	G protein beta WD-40 repeat protein
WD40_RFWD3	PF23419	66 - 157	2.9e-06	E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40	PF00400	66 - 100	1.6e-11	WD domain, G-beta repeat
Beta-prop_EML	PF23409	67 - 131	1.1e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_1st	PF24780	68 - 174	1.3e-11	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	72 - 152	3.8e-16	WDHD1 first WD40 domain
Beta-prop_IFT122_1st	PF23381	74 - 175	1.2e-08	IFT122 first beta-propeller
WDR90_beta-prop_4th	PF23342	75 - 206	9e-11	WDR90, 4th beta-propeller
Beta-prop_WDR75_1st	PF23869	80 - 144	1.2e-08	WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	86 - 190	7.5e-12	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	90 - 228	9.1e-12	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	93 - 281	1.2e-36	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	100 - 149	2.7e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	105 - 142	9.5e-10	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	106 - 193	3.2e-13	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	106 - 227	1.5e-18	WDR3 second beta-propeller domain
EIF3I	PF24805	109 - 153	8.2e-08	EIF3I
WD40_WDHD1_1st	PF24817	114 - 194	1.6e-14	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	122 - 282	3.6e-19	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	146 - 184	2.7e-10	WD domain, G-beta repeat
Beta-prop_Aladin	PF25460	150 - 281	1.5e-09	Aladin seven-bladed propeller
EIF3I	PF24805	152 - 246	5.1e-09	EIF3I
Beta-prop_WDR36-Utp21_1st	PF25171	172 - 316	7.2e-10	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	182 - 317	1.4e-20	CDC20/Fizzy WD40 domain
Beta-prop_NWD2_C	PF23586	195 - 307	1e-05	NWD2 C-terminal beta-propeller
Beta-prop_TEP1_2nd	PF25047	196 - 317	4e-10	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	197 - 316	7.7e-21	WDR3 first beta-propeller domain
Beta-prop_SCAP	PF24017	201 - 316	9.8e-07	SCAP Beta-propeller
EIF3I	PF24805	202 - 282	3.4e-06	EIF3I
Beta-prop_WDR3_2nd	PF25172	205 - 316	4.8e-11	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	208 - 301	9e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	222 - 304	2.6e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	231 - 271	4.9e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	255 - 318	4.3e-06	WDHD1 first WD40 domain
WD40	PF00400	277 - 315	5.3e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49660
fragaria_vesca	FvH4_1g18760
malus_domestica	MD01G1047000.v1.1 MD11G1213300.v1.1 MD15G1305300.v1.1 MD16G1282400.v1.1
prunus_persica	Prupe.6G211800_v2.0.a1
pyrus_communis	pycom01g07290 pycom15g26850 pycom16g25220
rosa_chinensis	RchiOBHm_Chr1g0324081 RchiOBHm_Chr2g0109631 RchiOBHm_Chr4g0412241 RchiOBHm_Chr7g0189251
rosa_laevigata	RLG00000017751 RLG00000018963 RLG00000020091 RLG00000024634 RLG00000029197 RLG00000032867 RLG00000034693 RLG00000035499
rosa_multiflora	Rmu_sc0000595.1_g000009 Rmu_sc0004483.1_g000044 Rmu_sc0010548.1_g000002
rosa_roxburghii	Rroxscaffold_2G00113400 Rroxscaffold_2G00134080
rosa_rugosa	Rorug02G0161400
rosa_samantha	Rh1AG321900 Rh2AG213600 Rh2BG224000 Rh2CG215800 Rh2CG303700 Rh2DG219100 Rh5DG065100 Rh5DG130500 Rh7DG103300
rosa_wichuraiana	Rw1G015840 Rw2G032920 Rw2G033190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	303
AccII	CGCG	1 cut(s)	125
AcoI	YGGCCR	4 cut(s)	100, 310, 334, 562
AcsI	RAATTY	2 cut(s)	86, 617
AcuI	CTGAAG	1 cut(s)	810
AcyI	GRCGYC	2 cut(s)	222, 300
AfaI	GTAC	2 cut(s)	638, 806
AfiI	CCNNNNNNNGG	2 cut(s)	217, 295
AgeI	ACCGGT	1 cut(s)	713
AgsI	TTSAA	5 cut(s)	28, 367, 448, 493, 687
AjnI	CCWGG	2 cut(s)	216, 545
AjuI	GAANNNNNNNTTGG	2 cut(s)	335, 367
AluBI	AGCT	2 cut(s)	525, 726
AluI	AGCT	2 cut(s)	525, 726
Alw21I	GWGCWC	2 cut(s)	728, 916
Alw26I	GTCTC	3 cut(s)	229, 268, 394
Alw44I	GTGCAC	1 cut(s)	912
AlwNI	CAGNNNCTG	1 cut(s)	546
AoxI	GGCC	7 cut(s)	58, 100, 208, 310, 324, 334, 562
ApaLI	GTGCAC	1 cut(s)	912
ApeKI	GCWGC	1 cut(s)	522
ApoI	RAATTY	2 cut(s)	86, 617
AsiGI	ACCGGT	1 cut(s)	713
AspLEI	GCGC	3 cut(s)	71, 359, 625
AspS9I	GGNCC	3 cut(s)	58, 325, 543
AsuC2I	CCSGG	1 cut(s)	436
AsuHPI	GGTGA	3 cut(s)	469, 875, 946
AsuII	TTCGAA	1 cut(s)	204
AvaII	GGWCC	1 cut(s)	543
BaeGI	GKGCMC	1 cut(s)	916
BalI	TGGCCA	2 cut(s)	336, 564
BanII	GRGCYC	1 cut(s)	728
BbsI	GAAGAC	1 cut(s)	343
Bbv12I	GWGCWC	2 cut(s)	728, 916
BbvI	GCAGC	1 cut(s)	534
BccI	CCATC	7 cut(s)	22, 80, 143, 191, 326, 494, 524
BceAI	ACGGC	2 cut(s)	115, 467
BciT130I	CCWGG	2 cut(s)	218, 547
BcnI	CCSGG	1 cut(s)	436
BcoDI	GTCTC	3 cut(s)	229, 268, 394
BfoI	RGCGCY	1 cut(s)	72
BglII	AGATCT	1 cut(s)	939
BisI	GCNGC	3 cut(s)	103, 192, 523
BlsI	GCNGC	3 cut(s)	104, 193, 524
Bme1390I	CCNGG	3 cut(s)	218, 436, 547
Bme18I	GGWCC	1 cut(s)	543
BmgT120I	GGNCC	3 cut(s)	58, 325, 543
BmiI	GGNNCC	3 cut(s)	12, 115, 397
BmrFI	CCNGG	3 cut(s)	218, 436, 547
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	1 cut(s)	541
BmuI	ACTGGG	1 cut(s)	699
BpiI	GAAGAC	1 cut(s)	343
Bpu14I	TTCGAA	1 cut(s)	204
BpuEI	CTTGAG	1 cut(s)	712
BpuMI	CCSGG	1 cut(s)	436
BsaBI	GATNNNNATC	2 cut(s)	492, 594
BsaHI	GRCGYC	2 cut(s)	222, 300
BsaI	GGTCTC	1 cut(s)	268
BsaJI	CCNNGG	6 cut(s)	109, 123, 216, 217, 331, 546
BsaWI	WCCGGW	4 cut(s)	77, 392, 601, 713
Bsc4I	CCNNNNNNNGG	2 cut(s)	217, 295
Bse118I	RCCGGY	3 cut(s)	308, 457, 713
Bse1I	ACTGG	2 cut(s)	565, 694
Bse8I	GATNNNNATC	2 cut(s)	492, 594
BseBI	CCWGG	2 cut(s)	218, 547
BseDI	CCNNGG	6 cut(s)	109, 123, 216, 217, 331, 546
BseGI	GGATG	6 cut(s)	14, 135, 430, 505, 556, 664
BseJI	GATNNNNATC	2 cut(s)	492, 594
BseLI	CCNNNNNNNGG	2 cut(s)	217, 295
BseMII	CTCAG	2 cut(s)	386, 659
BseNI	ACTGG	2 cut(s)	565, 694
BseRI	GAGGAG	3 cut(s)	82, 98, 160
BseSI	GKGCMC	1 cut(s)	916
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	1 cut(s)	534
BsgI	GTGCAG	2 cut(s)	553, 559
Bsh1236I	CGCG	1 cut(s)	125
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	7 cut(s)	60, 102, 210, 312, 326, 336, 564
BshTI	ACCGGT	1 cut(s)	713
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	2 cut(s)	728, 916
BsiSI	CCGG	7 cut(s)	78, 309, 393, 435, 458, 602, 714
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	2 cut(s)	217, 295
BsmAI	GTCTC	3 cut(s)	229, 268, 394
BsmBI	CGTCTC	2 cut(s)	229, 394
BsmFI	GGGAC	1 cut(s)	311
BsnI	GGCC	7 cut(s)	60, 102, 210, 312, 326, 336, 564
Bso31I	GGTCTC	1 cut(s)	268
Bsp119I	TTCGAA	1 cut(s)	204
Bsp1286I	GDGCHC	2 cut(s)	728, 916
Bsp143I	GATC	3 cut(s)	229, 384, 939
Bsp19I	CCATGG	1 cut(s)	331
BspANI	GGCC	7 cut(s)	60, 102, 210, 312, 326, 336, 564
BspCNI	CTCAG	2 cut(s)	385, 660
BspFNI	CGCG	1 cut(s)	125
BspLI	GGNNCC	3 cut(s)	12, 115, 397
BspT104I	TTCGAA	1 cut(s)	204
BspTNI	GGTCTC	1 cut(s)	268
BsrFI	RCCGGY	3 cut(s)	308, 457, 713
BsrI	ACTGG	2 cut(s)	565, 694
BssAI	RCCGGY	3 cut(s)	308, 457, 713
BssECI	CCNNGG	6 cut(s)	109, 123, 216, 217, 331, 546
BssMI	GATC	3 cut(s)	229, 384, 939
BssNI	GRCGYC	2 cut(s)	222, 300
BssT1I	CCWWGG	1 cut(s)	331
Bst2UI	CCWGG	2 cut(s)	218, 547
Bst4CI	ACNGT	3 cut(s)	412, 865, 934
BstACI	GRCGYC	2 cut(s)	222, 300
BstBI	TTCGAA	1 cut(s)	204
BstC8I	GCNNGC	1 cut(s)	459
BstDEI	CTNAG	3 cut(s)	372, 668, 948
BstDSI	CCRYGG	2 cut(s)	123, 331
BstF5I	GGATG	6 cut(s)	14, 135, 430, 505, 556, 664
BstFNI	CGCG	1 cut(s)	125
BstH2I	RGCGCY	1 cut(s)	72
BstHHI	GCGC	3 cut(s)	71, 359, 625
BstKTI	GATC	3 cut(s)	232, 387, 942
BstMAI	GTCTC	3 cut(s)	229, 268, 394
BstMBI	GATC	3 cut(s)	229, 384, 939
BstMCI	CGRYCG	1 cut(s)	103
BstMWI	GCNNNNNNNGC	3 cut(s)	66, 242, 911
BstNI	CCWGG	2 cut(s)	218, 547
BstSCI	CCNGG	3 cut(s)	216, 434, 545
BstSLI	GKGCMC	1 cut(s)	916
BstUI	CGCG	1 cut(s)	125
BstV1I	GCAGC	1 cut(s)	534
BstV2I	GAAGAC	1 cut(s)	343
BstX2I	RGATCY	1 cut(s)	939
BstYI	RGATCY	1 cut(s)	939
BstZI	CGGCCG	1 cut(s)	100
BsuRI	GGCC	7 cut(s)	60, 102, 210, 312, 326, 336, 564
BtgI	CCRYGG	2 cut(s)	123, 331
BtsCI	GGATG	6 cut(s)	14, 135, 430, 505, 556, 664
BtsI	GCAGTG	1 cut(s)	565
BtsIMutI	CAGTG	4 cut(s)	558, 565, 855, 861
Cac8I	GCNNGC	1 cut(s)	459
CaiI	CAGNNNCTG	1 cut(s)	546
CfoI	GCGC	3 cut(s)	71, 359, 625
Cfr10I	RCCGGY	3 cut(s)	308, 457, 713
Cfr13I	GGNCC	3 cut(s)	58, 325, 543
Cfr42I	CCGCGG	1 cut(s)	126
CseI	GACGC	1 cut(s)	211
Csp6I	GTAC	2 cut(s)	637, 805
CspAI	ACCGGT	1 cut(s)	713
CviAII	CATG	3 cut(s)	259, 332, 879
CviQI	GTAC	2 cut(s)	637, 805
DdeI	CTNAG	3 cut(s)	372, 668, 948
DpnI	GATC	3 cut(s)	231, 386, 941
DpnII	GATC	3 cut(s)	229, 384, 939
EaeI	YGGCCR	4 cut(s)	100, 310, 334, 562
EagI	CGGCCG	1 cut(s)	100
EciI	GGCGGA	4 cut(s)	178, 232, 256, 278
Ecl136II	GAGCTC	1 cut(s)	726
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	1 cut(s)	331
Eco24I	GRGCYC	1 cut(s)	728
Eco31I	GGTCTC	1 cut(s)	268
Eco47I	GGWCC	1 cut(s)	543
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	1 cut(s)	726
Eco57I	CTGAAG	1 cut(s)	810
EcoICRI	GAGCTC	1 cut(s)	726
EcoO109I	RGGNCCY	2 cut(s)	325, 543
EcoRII	CCWGG	2 cut(s)	216, 545
EcoT14I	CCWWGG	1 cut(s)	331
EcoT38I	GRGCYC	1 cut(s)	728
ErhI	CCWWGG	1 cut(s)	331
Esp3I	CGTCTC	2 cut(s)	229, 394
FaeI	CATG	3 cut(s)	262, 335, 882
FaiI	YATR	7 cut(s)	260, 333, 383, 772, 869, 880, 899
FaqI	GGGAC	1 cut(s)	311
FatI	CATG	3 cut(s)	258, 331, 878
Fnu4HI	GCNGC	3 cut(s)	103, 192, 523
FokI	GGATG	5 cut(s)	122, 437, 512, 563, 671
FriOI	GRGCYC	1 cut(s)	728
Fsp4HI	GCNGC	3 cut(s)	103, 192, 523
GlaI	GCGC	3 cut(s)	70, 358, 624
GluI	GCNGC	3 cut(s)	103, 192, 523
HaeII	RGCGCY	1 cut(s)	72
HaeIII	GGCC	7 cut(s)	60, 102, 210, 312, 326, 336, 564
HapII	CCGG	7 cut(s)	78, 309, 393, 435, 458, 602, 714
HgaI	GACGC	1 cut(s)	211
HhaI	GCGC	3 cut(s)	71, 359, 625
Hin1I	GRCGYC	2 cut(s)	222, 300
Hin1II	CATG	3 cut(s)	262, 335, 882
Hin6I	GCGC	3 cut(s)	69, 357, 623
HinP1I	GCGC	3 cut(s)	69, 357, 623
HinfI	GANTC	5 cut(s)	167, 248, 595, 732, 946
HpaII	CCGG	7 cut(s)	78, 309, 393, 435, 458, 602, 714
HphI	GGTGA	3 cut(s)	469, 875, 946
Hpy166II	GTNNAC	1 cut(s)	914
Hpy188I	TCNGA	7 cut(s)	97, 229, 274, 469, 669, 790, 840
Hpy188III	TCNNGA	6 cut(s)	430, 497, 676, 729, 943, 950
Hpy8I	GTNNAC	1 cut(s)	914
Hpy99I	CGWCG	4 cut(s)	101, 278, 305, 407
HpyAV	CCTTC	9 cut(s)	34, 166, 200, 211, 247, 409, 442, 823, 842
HpyCH4III	ACNGT	3 cut(s)	412, 865, 934
HpyCH4IV	ACGT	2 cut(s)	300, 348
HpyCH4V	TGCA	5 cut(s)	540, 570, 738, 820, 914
HpyF10VI	GCNNNNNNNGC	3 cut(s)	66, 242, 911
HpyF3I	CTNAG	3 cut(s)	372, 668, 948
HpySE526I	ACGT	2 cut(s)	300, 348
Hsp92I	GRCGYC	2 cut(s)	222, 300
Hsp92II	CATG	3 cut(s)	262, 335, 882
HspAI	GCGC	3 cut(s)	69, 357, 623
KroI	GCCGGC	1 cut(s)	457
KroNI	GCCGGC	1 cut(s)	459
KspI	CCGCGG	1 cut(s)	126
Kzo9I	GATC	3 cut(s)	229, 384, 939
Lsp1109I	GCAGC	1 cut(s)	534
LweI	GCATC	1 cut(s)	541
MaeII	ACGT	2 cut(s)	300, 348
MaeIII	GTNAC	5 cut(s)	475, 716, 757, 851, 881
MalI	GATC	3 cut(s)	231, 386, 941
MboI	GATC	3 cut(s)	229, 384, 939
MboII	GAAGA	4 cut(s)	343, 735, 803, 949
MflI	RGATCY	1 cut(s)	939
MhlI	GDGCHC	2 cut(s)	728, 916
MlsI	TGGCCA	2 cut(s)	336, 564
MluCI	AATT	6 cut(s)	86, 617, 796, 815, 842, 900
MluNI	TGGCCA	2 cut(s)	336, 564
MlyI	GAGTC	3 cut(s)	161, 242, 940
MmeI	TCCRAC	3 cut(s)	120, 297, 492
Mox20I	TGGCCA	2 cut(s)	336, 564
MroNI	GCCGGC	1 cut(s)	457
MscI	TGGCCA	2 cut(s)	336, 564
MseI	TTAA	2 cut(s)	53, 615
Msp20I	TGGCCA	2 cut(s)	336, 564
MspA1I	CMGCKG	1 cut(s)	125
MspI	CCGG	7 cut(s)	78, 309, 393, 435, 458, 602, 714
MspR9I	CCNGG	3 cut(s)	218, 436, 547
MvaI	CCWGG	2 cut(s)	218, 547
MvnI	CGCG	1 cut(s)	125
MwoI	GCNNNNNNNGC	3 cut(s)	66, 242, 911
NaeI	GCCGGC	1 cut(s)	459
NciI	CCSGG	1 cut(s)	436
NcoI	CCATGG	1 cut(s)	331
NdeII	GATC	3 cut(s)	229, 384, 939
NgoMIV	GCCGGC	1 cut(s)	457
NlaIII	CATG	3 cut(s)	262, 335, 882
NlaIV	GGNNCC	3 cut(s)	12, 115, 397
NmuCI	GTSAC	5 cut(s)	475, 716, 757, 851, 881
NspV	TTCGAA	1 cut(s)	204
PasI	CCCWGGG	1 cut(s)	217
PdiI	GCCGGC	1 cut(s)	459
PfeI	GAWTC	2 cut(s)	595, 732
PflFI	GACNNNGTC	1 cut(s)	473
PinAI	ACCGGT	1 cut(s)	713
PkrI	GCNGC	3 cut(s)	104, 193, 524
PleI	GAGTC	3 cut(s)	161, 242, 940
PpsI	GAGTC	3 cut(s)	161, 242, 940
PpuMI	RGGWCCY	1 cut(s)	543
Psp124BI	GAGCTC	1 cut(s)	728
Psp5II	RGGWCCY	1 cut(s)	543
Psp6I	CCWGG	2 cut(s)	216, 545
PspGI	CCWGG	2 cut(s)	216, 545
PspN4I	GGNNCC	3 cut(s)	12, 115, 397
PspPI	GGNCC	3 cut(s)	58, 325, 543
PspPPI	RGGWCCY	1 cut(s)	543
PstNI	CAGNNNCTG	1 cut(s)	546
PsuI	RGATCY	1 cut(s)	939
PsyI	GACNNNGTC	1 cut(s)	473
RsaI	GTAC	2 cut(s)	638, 806
RsaNI	GTAC	2 cut(s)	637, 805
SacI	GAGCTC	1 cut(s)	728
SacII	CCGCGG	1 cut(s)	126
SaqAI	TTAA	2 cut(s)	53, 615
SatI	GCNGC	3 cut(s)	103, 192, 523
Sau3AI	GATC	3 cut(s)	229, 384, 939
Sau96I	GGNCC	3 cut(s)	58, 325, 543
SchI	GAGTC	3 cut(s)	161, 242, 940
ScrFI	CCNGG	3 cut(s)	218, 436, 547
SduI	GDGCHC	2 cut(s)	728, 916
SfaNI	GCATC	1 cut(s)	541
Sfr303I	CCGCGG	1 cut(s)	126
SfuI	TTCGAA	1 cut(s)	204
SgrBI	CCGCGG	1 cut(s)	126
SinI	GGWCC	1 cut(s)	543
SmlI	CTYRAG	1 cut(s)	727
SmoI	CTYRAG	1 cut(s)	727
Sse9I	AATT	6 cut(s)	86, 617, 796, 815, 842, 900
SstI	GAGCTC	1 cut(s)	728
StyD4I	CCNGG	3 cut(s)	216, 434, 545
StyI	CCWWGG	1 cut(s)	331
TaaI	ACNGT	3 cut(s)	412, 865, 934
TaiI	ACGT	2 cut(s)	303, 351
TaqI	TCGA	4 cut(s)	5, 204, 402, 518
TasI	AATT	6 cut(s)	86, 617, 796, 815, 842, 900
TatI	WGTACW	2 cut(s)	636, 804
TauI	GCSGC	2 cut(s)	105, 194
TfiI	GAWTC	2 cut(s)	595, 732
Tru1I	TTAA	2 cut(s)	53, 615
Tru9I	TTAA	2 cut(s)	53, 615
TscAI	CASTG	4 cut(s)	565, 572, 862, 868
TseFI	GTSAC	5 cut(s)	475, 716, 757, 851, 881
TseI	GCWGC	1 cut(s)	522
Tsp45I	GTSAC	5 cut(s)	475, 716, 757, 851, 881
TspDTI	ATGAA	1 cut(s)	247
TspRI	CASTG	4 cut(s)	565, 572, 862, 868
Tth111I	GACNNNGTC	1 cut(s)	473
VneI	GTGCAC	1 cut(s)	912
VpaK11BI	GGWCC	1 cut(s)	543
XapI	RAATTY	2 cut(s)	86, 617
ZraI	GACGTC	1 cut(s)	301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.