RLG00000024634

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002681
Physical Location & Seq
Reverse (-)
37651779 .. 37652349
571 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024634

Sequence Viewer

Length: 423 bp
ATGGACCTGCTTCCCAGGCCTGGCCAATCGAACCTCCGTGGCCTTACGACGCCATTTGATTTGATTCTCAGATCCCTCACCAATCGGGGATTTGACAATCCAGATCTGTCTTTTTGTATTTGTTGTGATCTTGGTCTGAATTGGCTGTGGCGGAGGTCGGTTGTGGCACAATCGGGACCCGTCACTTTTTCTGGAAGATGCGATGGCTTCGGGGACGATTTTGAACTACAGACGGTGACGAGGTTTTGGTTTCGTGGTCTGACTTTGTACACGAGATCGGTCTCGATTGTGAATTGTTTGGATTGCTTGGTGTGCTGCTTCTCTATTGCCGTCAATGGTGATGGTAATATCGATGGCGTCTTACATGTCCGGTGTTCTGGGTATGCAATGGGTTTTCTGGAGGGTCGGCGGTGGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.77

Weight (kDa)

8.12

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AciI CCGC 2 cut(s) 151, 409
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 22
AcyI GRCGYC 2 cut(s) 50, 357
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 20
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 2 cut(s) 14, 19
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 66
AoxI GGCC 3 cut(s) 17, 22, 40
ApeKI GCWGC 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 4, 176
AsuHPI GGTGA 3 cut(s) 70, 247, 350
AvaII GGWCC 2 cut(s) 4, 176
BalI TGGCCA 1 cut(s) 24
BauI CACGAG 1 cut(s) 271
BbvI GCAGC 1 cut(s) 302
BccI CCATC 3 cut(s) 197, 335, 347
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 2 cut(s) 16, 21
BcoDI GTCTC 1 cut(s) 286
BfmI CTRYAG 1 cut(s) 227
BfuAI ACCTGC 1 cut(s) 15
BglII AGATCT 1 cut(s) 103
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme1390I CCNGG 2 cut(s) 16, 21
Bme18I GGWCC 2 cut(s) 4, 176
BmgT120I GGNCC 2 cut(s) 4, 176
BmiI GGNNCC 2 cut(s) 177, 178
BmrFI CCNGG 2 cut(s) 16, 21
BmsI GCATC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 419
Bsa29I ATCGAT 1 cut(s) 351
BsaHI GRCGYC 2 cut(s) 50, 357
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 14, 37
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 2 cut(s) 16, 21
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 14, 37
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 302
BshFI GGCC 3 cut(s) 19, 24, 42
BshVI ATCGAT 1 cut(s) 351
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 2 cut(s) 189, 227
BslI CCNNNNNNNGG 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 189, 227
BsnI GGCC 3 cut(s) 19, 24, 42
Bso31I GGTCTC 1 cut(s) 286
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 4 cut(s) 71, 103, 127, 275
BspACI CCGC 2 cut(s) 151, 409
BspANI GGCC 3 cut(s) 19, 24, 42
BspCNI CTCAG 1 cut(s) 81
BspDI ATCGAT 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 177, 178
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 1 cut(s) 66
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 393
BsrGI TGTACA 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 14, 37
BssMI GATC 4 cut(s) 71, 103, 127, 275
BssNI GRCGYC 2 cut(s) 50, 357
BssSI CACGAG 1 cut(s) 271
Bst2BI CACGAG 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 16, 21
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 2 cut(s) 50, 357
BstAUI TGTACA 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 37
BstKTI GATC 4 cut(s) 74, 106, 130, 278
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 4 cut(s) 71, 103, 127, 275
BstMWI GCNNNNNNNGC 2 cut(s) 16, 312
BstNI CCWGG 2 cut(s) 16, 21
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 14, 19
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 302
BstX2I RGATCY 2 cut(s) 71, 103
BstYI RGATCY 2 cut(s) 71, 103
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 3 cut(s) 19, 24, 42
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 37
BtgZI GCGATG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 4, 176
ClaI ATCGAT 1 cut(s) 351
CseI GACGC 2 cut(s) 58, 346
Csp6I GTAC 1 cut(s) 268
CviAII CATG 1 cut(s) 365
CviJI RGCY 5 cut(s) 19, 24, 42, 145, 207
CviKI_1 RGCY 5 cut(s) 19, 24, 42, 145, 207
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 1 cut(s) 68
DpnI GATC 4 cut(s) 73, 105, 129, 277
DpnII GATC 4 cut(s) 71, 103, 127, 275
EaeI YGGCCR 1 cut(s) 22
EciI GGCGGA 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 2 cut(s) 4, 176
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 14, 19
FaeI CATG 1 cut(s) 368
FaiI YATR 2 cut(s) 366, 384
FaqI GGGAC 2 cut(s) 189, 227
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 316
Fsp4HI GCNGC 1 cut(s) 316
GluI GCNGC 1 cut(s) 316
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 3 cut(s) 19, 24, 42
HapII CCGG 1 cut(s) 370
HgaI GACGC 2 cut(s) 58, 346
Hin1I GRCGYC 2 cut(s) 50, 357
Hin1II CATG 1 cut(s) 368
HinfI GANTC 1 cut(s) 64
HpaII CCGG 1 cut(s) 370
HphI GGTGA 3 cut(s) 70, 247, 350
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 3 cut(s) 71, 138, 261
Hpy188III TCNNGA 5 cut(s) 101, 174, 192, 283, 398
Hpy8I GTNNAC 1 cut(s) 270
Hpy99I CGWCG 1 cut(s) 52
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 312
HpyF3I CTNAG 1 cut(s) 68
Hsp92I GRCGYC 2 cut(s) 50, 357
Hsp92II CATG 1 cut(s) 368
KflI GGGWCCC 1 cut(s) 176
Kzo9I GATC 4 cut(s) 71, 103, 127, 275
LpnPI CCDG 9 cut(s) 6, 20, 28, 33, 114, 177, 363, 383, 383
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 181, 235
MalI GATC 4 cut(s) 73, 105, 129, 277
MboI GATC 4 cut(s) 71, 103, 127, 275
MboII GAAGA 1 cut(s) 207
MflI RGATCY 2 cut(s) 71, 103
MlsI TGGCCA 1 cut(s) 24
MluCI AATT 2 cut(s) 139, 292
MluNI TGGCCA 1 cut(s) 24
MnlI CCTC 6 cut(s) 44, 86, 147, 234, 394, 408
Mox20I TGGCCA 1 cut(s) 24
MscI TGGCCA 1 cut(s) 24
Msp20I TGGCCA 1 cut(s) 24
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 2 cut(s) 16, 21
MvaI CCWGG 2 cut(s) 16, 21
MwoI GCNNNNNNNGC 2 cut(s) 16, 312
NdeII GATC 4 cut(s) 71, 103, 127, 275
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 2 cut(s) 177, 178
NmuCI GTSAC 2 cut(s) 181, 235
NspI RCATGY 1 cut(s) 368
PceI AGGCCT 1 cut(s) 19
PciI ACATGT 1 cut(s) 364
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 317
PpuMI RGGWCCY 1 cut(s) 176
PscI ACATGT 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 14, 19
PspGI CCWGG 2 cut(s) 14, 19
PspN4I GGNNCC 2 cut(s) 177, 178
PspPI GGNCC 2 cut(s) 4, 176
PspPPI RGGWCCY 1 cut(s) 176
PsuI RGATCY 2 cut(s) 71, 103
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 4 cut(s) 71, 103, 127, 275
Sau96I GGNCC 2 cut(s) 4, 176
ScrFI CCNGG 2 cut(s) 16, 21
SetI ASST 4 cut(s) 9, 36, 158, 245
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 227
SinI GGWCC 2 cut(s) 4, 176
Sse9I AATT 2 cut(s) 139, 292
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 151, 409
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 14, 19
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 3 cut(s) 29, 284, 351
TaqII GACCGA 1 cut(s) 268
TasI AATT 2 cut(s) 139, 292
TatI WGTACW 1 cut(s) 267
TfiI GAWTC 1 cut(s) 64
TseFI GTSAC 2 cut(s) 181, 235
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 2 cut(s) 181, 235
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 2 cut(s) 4, 176
XceI RCATGY 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.