MD12G1170000.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002774
Physical Location & Seq
Reverse (-)
25102666 .. 25103433
768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1170000.v1.1.491

Sequence Viewer

Length: 747 bp
ATGACAAACGGCTCTCACAGCGGCAATGCGTCGAACACATTCGAGGCATTCTACGAGGGCTGGCTAGTCCAGCAAGAACACTTCCTCGATGAGCTCTTATCGGCCCAGCAGACGATCGACGAGGCCCGAGATGAAGACCTCCGAGATTTGGTGTCTCGGGTTCTCTTCCATTACCAGCAGTATTACGACGAGAAATCGCGACTTGGACAGCGGGATGTTTTACTGGTTTTCTCGCCGACGTGGTATACTTCCTACGAAAGGAGCCTGCTTTGGATTGCCGGGTACAAACCGGGTATCGTATTCCGGCTCGTGACCGAGTCGGTGCCCGATTTGAGCGACCAGCAGCGGGTCAGGATGGCCCGGCCGAGGGAGGCGACCCGGGTCGAGGAGCGCGCGCTCACCGACAAGCTCGCCAAGATTCACGAGACCGTGGCGGCGCCGCCGTTCATGGATGCGGTGCGGCGGTACGGGAGGGCCAGGCACGGGGATATCGTGGAGGACGACGCGGTGATCAAGTCCCTTAAGTCGGCATTGGAGACCGTGGTGGAGAATGCCAACTTGCCGAGGACGACGATGGCGATGAAGCTGGTGGATTTGCTAAGCTCGGGGCAGGCAGTGAGGTTTTTGACGGCGGTGATGCAGTTTCAGTTAAGGATTCGGAGTTTGAGGTTGGAGAGGGACGCCGAGAAGCAGCGGGAAATGAGCGGAGGCGGTGGTGGTTGGAATCCAATTAGTAGTAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

249

Amino Acids

28.45

Weight (kDa)

6.25

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 29 - 105 3.8e-22 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 322, 436
AccBSI CCGCTC 1 cut(s) 705
AccI GTMKAC 1 cut(s) 245
AccII CGCG 4 cut(s) 199, 393, 395, 506
AcoI YGGCCR 1 cut(s) 362
AcyI GRCGYC 2 cut(s) 437, 681
AfaI GTAC 2 cut(s) 284, 467
AfiI CCNNNNNNNGG 8 cut(s) 148, 258, 346, 366, 367, 385, 483, 526
AflII CTTAAG 1 cut(s) 521
AjiI CACGTC 1 cut(s) 240
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 4 cut(s) 94, 409, 586, 603
AluI AGCT 4 cut(s) 94, 409, 586, 603
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 3 cut(s) 159, 419, 530
Ama87I CYCGRG 4 cut(s) 126, 156, 378, 604
AoxI GGCC 5 cut(s) 102, 123, 357, 362, 474
ApeKI GCWGC 2 cut(s) 343, 691
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 4 cut(s) 393, 395, 397, 439
AspS9I GGNCC 4 cut(s) 103, 124, 358, 474
AsuC2I CCSGG 5 cut(s) 280, 291, 361, 379, 380
AsuHPI GGTGA 3 cut(s) 391, 520, 646
AvaI CYCGRG 4 cut(s) 126, 156, 378, 604
BaeGI GKGCMC 1 cut(s) 327
BanI GGYRCC 2 cut(s) 322, 436
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 2 cut(s) 308, 422
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 355, 703
BccI CCATC 2 cut(s) 349, 568
BceAI ACGGC 3 cut(s) 25, 427, 645
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 5 cut(s) 280, 291, 361, 379, 380
BcoDI GTCTC 3 cut(s) 159, 419, 530
BfaI CTAG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 440
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 6 cut(s) 22, 344, 435, 440, 461, 692
BlpI GCTNAGC 1 cut(s) 599
BlsI GCNGC 6 cut(s) 23, 345, 436, 441, 462, 693
Bme1390I CCNGG 6 cut(s) 280, 291, 361, 379, 380, 478
BmeT110I CYCGRG 4 cut(s) 126, 156, 378, 604
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 4 cut(s) 103, 124, 358, 474
BmiI GGNNCC 3 cut(s) 263, 324, 438
BmrFI CCNGG 6 cut(s) 280, 291, 361, 379, 380, 478
BmsI GCATC 2 cut(s) 442, 627
BoxI GACNNNNGTC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 599
BpuMI CCSGG 5 cut(s) 280, 291, 361, 379, 380
BsaHI GRCGYC 2 cut(s) 437, 681
BsaI GGTCTC 2 cut(s) 419, 530
BsaJI CCNNGG 5 cut(s) 365, 378, 429, 540, 563
Bsc4I CCNNNNNNNGG 8 cut(s) 148, 258, 346, 366, 367, 385, 483, 526
Bse1I ACTGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 5 cut(s) 365, 378, 429, 540, 563
BseGI GGATG 3 cut(s) 220, 360, 457
BseLI CCNNNNNNNGG 8 cut(s) 148, 258, 346, 366, 367, 385, 483, 526
BseMI GCAATG 1 cut(s) 31
BseNI ACTGG 1 cut(s) 228
BsePI GCGCGC 2 cut(s) 391, 393
BseRI GAGGAG 1 cut(s) 401
BseSI GKGCMC 1 cut(s) 327
BseX3I CGGCCG 1 cut(s) 362
BseXI GCAGC 2 cut(s) 355, 703
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 4 cut(s) 199, 393, 395, 506
Bsh1285I CGRYCG 2 cut(s) 117, 365
BshFI GGCC 5 cut(s) 104, 125, 359, 364, 476
BshNI GGYRCC 2 cut(s) 322, 436
BsiEI CGRYCG 2 cut(s) 117, 365
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 4 cut(s) 126, 156, 378, 604
BsiSI CCGG 5 cut(s) 279, 290, 304, 361, 379
BslFI GGGAC 2 cut(s) 502, 692
BslI CCNNNNNNNGG 8 cut(s) 148, 258, 346, 366, 367, 385, 483, 526
BsmAI GTCTC 3 cut(s) 159, 419, 530
BsmFI GGGAC 2 cut(s) 502, 692
BsmI GAATGC 2 cut(s) 47, 556
BsnI GGCC 5 cut(s) 104, 125, 359, 364, 476
Bso31I GGTCTC 2 cut(s) 419, 530
BsoBI CYCGRG 4 cut(s) 126, 156, 378, 604
Bsp1286I GDGCHC 2 cut(s) 96, 327
Bsp143I GATC 2 cut(s) 114, 510
Bsp1720I GCTNAGC 1 cut(s) 599
Bsp68I TCGCGA 1 cut(s) 199
BspANI GGCC 5 cut(s) 104, 125, 359, 364, 476
BspFNI CGCG 4 cut(s) 199, 393, 395, 506
BspLI GGNNCC 3 cut(s) 263, 324, 438
BspT107I GGYRCC 2 cut(s) 322, 436
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 419, 530
BsrBI CCGCTC 1 cut(s) 705
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 1 cut(s) 228
BssECI CCNNGG 5 cut(s) 365, 378, 429, 540, 563
BssHII GCGCGC 2 cut(s) 391, 393
BssMI GATC 2 cut(s) 114, 510
BssNAI GTATAC 1 cut(s) 246
BssNI GRCGYC 2 cut(s) 437, 681
BssSI CACGAG 2 cut(s) 308, 422
Bst1107I GTATAC 1 cut(s) 246
Bst2BI CACGAG 2 cut(s) 308, 422
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 430, 541
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 2 cut(s) 437, 681
BstAFI CTTAAG 1 cut(s) 521
BstC8I GCNNGC 6 cut(s) 62, 266, 393, 395, 411, 612
BstDEI CTNAG 1 cut(s) 599
BstDSI CCRYGG 2 cut(s) 429, 540
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 3 cut(s) 220, 360, 457
BstFNI CGCG 4 cut(s) 199, 393, 395, 506
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 4 cut(s) 393, 395, 397, 439
BstKTI GATC 2 cut(s) 117, 513
BstMAI GTCTC 3 cut(s) 159, 419, 530
BstMBI GATC 2 cut(s) 114, 510
BstMCI CGRYCG 2 cut(s) 117, 365
BstMWI GCNNNNNNNGC 2 cut(s) 18, 70
BstNI CCWGG 1 cut(s) 478
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 6 cut(s) 278, 289, 359, 377, 378, 476
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 4 cut(s) 199, 393, 395, 506
BstV1I GCAGC 2 cut(s) 355, 703
BstV2I GAAGAC 1 cut(s) 141
BstZ17I GTATAC 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 362
BsuRI GGCC 5 cut(s) 104, 125, 359, 364, 476
BtgI CCRYGG 2 cut(s) 429, 540
BtgZI GCGATG 1 cut(s) 593
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 3 cut(s) 220, 360, 457
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 1 cut(s) 621
BtuMI TCGCGA 1 cut(s) 199
Cac8I GCNNGC 6 cut(s) 62, 266, 393, 395, 411, 612
CfoI GCGC 4 cut(s) 393, 395, 397, 439
Cfr13I GGNCC 4 cut(s) 103, 124, 358, 474
Cfr9I CCCGGG 1 cut(s) 378
CseI GACGC 3 cut(s) 18, 512, 689
Csp6I GTAC 2 cut(s) 283, 466
CviAII CATG 1 cut(s) 448
CviQI GTAC 2 cut(s) 283, 466
DdeI CTNAG 1 cut(s) 599
DinI GGCGCC 1 cut(s) 438
DpnI GATC 2 cut(s) 116, 512
DpnII GATC 2 cut(s) 114, 510
EaeI YGGCCR 1 cut(s) 362
EagI CGGCCG 1 cut(s) 362
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 94
EclXI CGGCCG 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 2 cut(s) 419, 530
Eco32I GATATC 1 cut(s) 490
Eco52I CGGCCG 1 cut(s) 362
Eco53kI GAGCTC 1 cut(s) 94
Eco88I CYCGRG 4 cut(s) 126, 156, 378, 604
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 96
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
FaeI CATG 1 cut(s) 451
FaiI YATR 2 cut(s) 246, 449
FaqI GGGAC 2 cut(s) 502, 692
FatI CATG 1 cut(s) 447
FauI CCCGC 3 cut(s) 204, 339, 687
FbaI TGATCA 1 cut(s) 510
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 6 cut(s) 22, 344, 435, 440, 461, 692
FokI GGATG 3 cut(s) 227, 367, 464
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 6 cut(s) 22, 344, 435, 440, 461, 692
FspBI CTAG 1 cut(s) 65
GlaI GCGC 4 cut(s) 392, 394, 396, 438
GluI GCNGC 6 cut(s) 22, 344, 435, 440, 461, 692
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 5 cut(s) 104, 125, 359, 364, 476
HapII CCGG 5 cut(s) 279, 290, 304, 361, 379
HgaI GACGC 3 cut(s) 18, 512, 689
HhaI GCGC 4 cut(s) 393, 395, 397, 439
Hin1I GRCGYC 2 cut(s) 437, 681
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 4 cut(s) 391, 393, 395, 437
HinP1I GCGC 4 cut(s) 391, 393, 395, 437
HinfI GANTC 4 cut(s) 317, 418, 655, 724
HpaII CCGG 5 cut(s) 279, 290, 304, 361, 379
HphI GGTGA 3 cut(s) 391, 520, 646
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 2 cut(s) 143, 660
Hpy188III TCNNGA 4 cut(s) 198, 310, 352, 422
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 6 cut(s) 34, 122, 191, 241, 506, 574
HpyCH4III ACNGT 2 cut(s) 430, 541
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 1 cut(s) 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 70
HpyF3I CTNAG 1 cut(s) 599
HpySE526I ACGT 1 cut(s) 239
Hsp92I GRCGYC 2 cut(s) 437, 681
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 4 cut(s) 391, 393, 395, 437
KasI GGCGCC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 2 cut(s) 114, 510
LmnI GCTCC 2 cut(s) 261, 388
Lsp1109I GCAGC 2 cut(s) 355, 703
LweI GCATC 2 cut(s) 442, 627
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 116, 512
MbiI CCGCTC 1 cut(s) 705
MboI GATC 2 cut(s) 114, 510
MboII GAAGA 2 cut(s) 146, 157
MhlI GDGCHC 2 cut(s) 96, 327
MluCI AATT 1 cut(s) 729
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 651, 701
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 2 cut(s) 522, 650
MspA1I CMGCKG 4 cut(s) 21, 211, 346, 694
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 5 cut(s) 279, 290, 304, 361, 379
MspR9I CCNGG 6 cut(s) 280, 291, 361, 379, 380, 478
Mva1269I GAATGC 2 cut(s) 47, 556
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 4 cut(s) 199, 393, 395, 506
MwoI GCNNNNNNNGC 2 cut(s) 18, 70
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 5 cut(s) 280, 291, 361, 379, 380
NdeII GATC 2 cut(s) 114, 510
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 3 cut(s) 263, 324, 438
NmeAIII GCCGAG 3 cut(s) 390, 588, 709
NmuCI GTSAC 1 cut(s) 310
NruI TCGCGA 1 cut(s) 199
PauI GCGCGC 2 cut(s) 391, 393
PcsI WCGNNNNNNNCGW 3 cut(s) 489, 498, 575
PctI GAATGC 2 cut(s) 47, 556
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 3 cut(s) 418, 655, 724
PflFI GACNNNGTC 1 cut(s) 316
PkrI GCNGC 6 cut(s) 23, 345, 436, 441, 462, 693
Ple19I CGATCG 1 cut(s) 117
PleI GAGTC 1 cut(s) 325
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 325
PshAI GACNNNNGTC 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 3 cut(s) 263, 324, 438
PspPI GGNCC 4 cut(s) 103, 124, 358, 474
PsyI GACNNNGTC 1 cut(s) 316
PteI GCGCGC 2 cut(s) 391, 393
PvuI CGATCG 1 cut(s) 117
RruI TCGCGA 1 cut(s) 199
RsaI GTAC 2 cut(s) 284, 467
RsaNI GTAC 2 cut(s) 283, 466
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 522, 650
SatI GCNGC 6 cut(s) 22, 344, 435, 440, 461, 692
Sau3AI GATC 2 cut(s) 114, 510
Sau96I GGNCC 4 cut(s) 103, 124, 358, 474
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 6 cut(s) 280, 291, 361, 379, 380, 478
SduI GDGCHC 2 cut(s) 96, 327
SetI ASST 9 cut(s) 96, 141, 242, 411, 588, 605, 623, 671, 743
SfaNI GCATC 2 cut(s) 442, 627
SfoI GGCGCC 1 cut(s) 438
SmaI CCCGGG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 1 cut(s) 729
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 6 cut(s) 278, 289, 359, 377, 378, 476
TaaI ACNGT 2 cut(s) 430, 541
TaiI ACGT 1 cut(s) 242
TaqI TCGA 5 cut(s) 32, 42, 87, 117, 384
TaqII GACCGA 1 cut(s) 329
TasI AATT 1 cut(s) 729
TauI GCSGC 4 cut(s) 24, 437, 442, 463
TfiI GAWTC 3 cut(s) 418, 655, 724
Tru1I TTAA 2 cut(s) 522, 650
Tru9I TTAA 2 cut(s) 522, 650
TscAI CASTG 1 cut(s) 621
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 343, 691
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 147, 436, 596
TspMI CCCGGG 1 cut(s) 378
TspRI CASTG 1 cut(s) 621
Tth111I GACNNNGTC 1 cut(s) 316
Vha464I CTTAAG 1 cut(s) 521
XagI CCTNNNNNAGG 1 cut(s) 256
XmaI CCCGGG 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.