pycom04g14060

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002774
Physical Location & Seq
Reverse (-)
16949186 .. 16949941
756 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14060.1

Sequence Viewer

Length: 756 bp
ATGTCAACCGGCTCTCACAGCGGCAATACATCGAACACATTCGTGGCATTCTTCGAGGGCTGGCTGGTCCGGCAAGAACACTTCCTCGACGAGCTCCTATCGACCCAGCAGACAATCGACGAGGCCCGAGATGAAGACCTTCGAGATTTGATATCCCGGGTTCTCCTCCATTACCAGCAGTACTACGACGAGAAATCGCGACTTGCCCATCGGGATGTTTTCCTGGTTTTCTCCCCGAAGTGGTTTAGTTCCTACGAAAGGACCCTCCTTTGGATCGCCGGGTACAAACCGGGTCTCGTATTCCGGATAGTGACGGAGTCGGTGCCCGATTTGAGCGACCGGCAGCGGGTCAATATAGCCCGGCTGAGGGAGGAGACCCGGGTCGAGGAGCGCGCGCTCAACGACAAGCTTGCCAAGATTCACGAGAGCGTGGCGGCGCCGCCGTTCGTCGACGTGCTGCGGCAGTACGGGAGGGCCAGGGACGGGGGGATCGTGGAGGACATCGCGGTGATCGAATCGCTGAAGCCGGCATTGGAGTCCGTGCTGGTGAATGCCAACTTGCTGAGGACGACGATGGTGACGAAGCTGGTGGAGATACTTAGCTCGAGGCAGGCGGTGAGGTTTTTGACGGCGGTGGCGCAGTTTCAGCTGAAGATTAGGAGTTTGGGGTTGGAGAGGGACGCCGAGAAGCGGAGGGAATTGAGCGGAAGGAGCGGCGGCGGTGGGAGTCCAATTGGTAGTACTAGTAGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

252

Amino Acids

28.58

Weight (kDa)

7.99

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 31 - 105 7.2e-23 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 322, 436
AccBSI CCGCTC 2 cut(s) 705, 714
AccI GTMKAC 1 cut(s) 450
AccII CGCG 4 cut(s) 199, 393, 395, 506
AccIII TCCGGA 1 cut(s) 303
AclWI GGATC 2 cut(s) 281, 497
AcuI CTGAAG 2 cut(s) 542, 671
AcyI GRCGYC 2 cut(s) 437, 681
AfaI GTAC 4 cut(s) 182, 284, 467, 742
AfiI CCNNNNNNNGG 9 cut(s) 240, 258, 270, 346, 366, 367, 385, 483, 690
AhlI ACTAGT 1 cut(s) 743
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 2 cut(s) 222, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 5 cut(s) 94, 409, 586, 603, 649
AluI AGCT 5 cut(s) 94, 409, 586, 603, 649
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 299, 368
AlwI GGATC 2 cut(s) 281, 497
Ama87I CYCGRG 4 cut(s) 126, 156, 378, 604
Aor13HI TCCGGA 1 cut(s) 303
AoxI GGCC 2 cut(s) 123, 474
ApeKI GCWGC 2 cut(s) 343, 457
Asp700I GAANNNNTTC 2 cut(s) 38, 138
AspLEI GCGC 5 cut(s) 393, 395, 397, 439, 640
AspS9I GGNCC 4 cut(s) 67, 124, 261, 474
AsuC2I CCSGG 7 cut(s) 157, 158, 280, 291, 361, 379, 380
AsuHPI GGTGA 4 cut(s) 520, 559, 589, 628
AvaI CYCGRG 4 cut(s) 126, 156, 378, 604
AvaII GGWCC 2 cut(s) 67, 261
BaeGI GKGCMC 1 cut(s) 327
BanI GGYRCC 2 cut(s) 322, 436
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 2 cut(s) 365, 563
BbvI GCAGC 2 cut(s) 355, 444
BccI CCATC 2 cut(s) 216, 568
BceAI ACGGC 2 cut(s) 427, 645
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 2 cut(s) 224, 478
BcnI CCSGG 7 cut(s) 157, 158, 280, 291, 361, 379, 380
BcoDI GTCTC 2 cut(s) 299, 368
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 1 cut(s) 744
BfoI RGCGCY 1 cut(s) 440
BisI GCNGC 8 cut(s) 22, 344, 435, 440, 458, 461, 715, 718
BlsI GCNGC 8 cut(s) 23, 345, 436, 441, 459, 462, 716, 719
BmcAI AGTACT 2 cut(s) 182, 742
Bme1390I CCNGG 9 cut(s) 157, 158, 224, 280, 291, 361, 379, 380, 478
Bme18I GGWCC 2 cut(s) 67, 261
BmeT110I CYCGRG 4 cut(s) 126, 156, 378, 604
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 4 cut(s) 67, 124, 261, 474
BmiI GGNNCC 3 cut(s) 263, 324, 438
BmrFI CCNGG 9 cut(s) 157, 158, 224, 280, 291, 361, 379, 380, 478
BoxI GACNNNNGTC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 141
Bpu10I CCTNAGC 2 cut(s) 365, 563
BpuMI CCSGG 7 cut(s) 157, 158, 280, 291, 361, 379, 380
BsaHI GRCGYC 2 cut(s) 437, 681
BsaI GGTCTC 2 cut(s) 299, 368
BsaJI CCNNGG 3 cut(s) 156, 378, 477
BsaWI WCCGGW 1 cut(s) 303
Bsc4I CCNNNNNNNGG 9 cut(s) 240, 258, 270, 346, 366, 367, 385, 483, 690
Bse118I RCCGGY 3 cut(s) 8, 339, 526
BseAI TCCGGA 1 cut(s) 303
BseBI CCWGG 2 cut(s) 224, 478
BseDI CCNNGG 3 cut(s) 156, 378, 477
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 9 cut(s) 240, 258, 270, 346, 366, 367, 385, 483, 690
BseMII CTCAG 2 cut(s) 356, 554
BsePI GCGCGC 2 cut(s) 391, 393
BseRI GAGGAG 3 cut(s) 155, 386, 401
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 2 cut(s) 355, 444
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 4 cut(s) 199, 393, 395, 506
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 2 cut(s) 125, 476
BshNI GGYRCC 2 cut(s) 322, 436
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 4 cut(s) 126, 156, 378, 604
BslFI GGGAC 2 cut(s) 494, 692
BslI CCNNNNNNNGG 9 cut(s) 240, 258, 270, 346, 366, 367, 385, 483, 690
BsmAI GTCTC 2 cut(s) 299, 368
BsmFI GGGAC 2 cut(s) 494, 692
BsmI GAATGC 2 cut(s) 47, 556
BsnI GGCC 2 cut(s) 125, 476
Bso31I GGTCTC 2 cut(s) 299, 368
BsoBI CYCGRG 4 cut(s) 126, 156, 378, 604
Bsp1286I GDGCHC 2 cut(s) 96, 327
Bsp13I TCCGGA 1 cut(s) 303
Bsp143I GATC 3 cut(s) 273, 489, 510
Bsp68I TCGCGA 1 cut(s) 199
BspANI GGCC 2 cut(s) 125, 476
BspCNI CTCAG 2 cut(s) 357, 555
BspEI TCCGGA 1 cut(s) 303
BspFNI CGCG 4 cut(s) 199, 393, 395, 506
BspLI GGNNCC 3 cut(s) 263, 324, 438
BspPI GGATC 2 cut(s) 281, 497
BspT107I GGYRCC 2 cut(s) 322, 436
BspTNI GGTCTC 2 cut(s) 299, 368
BsrBI CCGCTC 2 cut(s) 705, 714
BsrFI RCCGGY 3 cut(s) 8, 339, 526
BssAI RCCGGY 3 cut(s) 8, 339, 526
BssECI CCNNGG 3 cut(s) 156, 378, 477
BssHII GCGCGC 2 cut(s) 391, 393
BssMI GATC 3 cut(s) 273, 489, 510
BssNI GRCGYC 2 cut(s) 437, 681
BssSI CACGAG 1 cut(s) 422
Bst2BI CACGAG 1 cut(s) 422
Bst2UI CCWGG 2 cut(s) 224, 478
BstACI GRCGYC 2 cut(s) 437, 681
BstC8I GCNNGC 6 cut(s) 62, 393, 395, 411, 528, 612
BstDEI CTNAG 3 cut(s) 365, 563, 599
BstENI CCTNNNNNAGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 220
BstFNI CGCG 4 cut(s) 199, 393, 395, 506
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 5 cut(s) 393, 395, 397, 439, 640
BstKTI GATC 3 cut(s) 276, 492, 513
BstMAI GTCTC 2 cut(s) 299, 368
BstMBI GATC 3 cut(s) 273, 489, 510
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 18, 70, 646, 711
BstNI CCWGG 2 cut(s) 224, 478
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 9 cut(s) 155, 156, 222, 278, 289, 359, 377, 378, 476
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 4 cut(s) 199, 393, 395, 506
BstV1I GCAGC 2 cut(s) 355, 444
BstV2I GAAGAC 1 cut(s) 141
BsuRI GGCC 2 cut(s) 125, 476
BtgZI GCGATG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 1 cut(s) 220
BtuMI TCGCGA 1 cut(s) 199
Cac8I GCNNGC 6 cut(s) 62, 393, 395, 411, 528, 612
CfoI GCGC 5 cut(s) 393, 395, 397, 439, 640
Cfr10I RCCGGY 3 cut(s) 8, 339, 526
Cfr13I GGNCC 4 cut(s) 67, 124, 261, 474
Cfr9I CCCGGG 2 cut(s) 156, 378
CseI GACGC 1 cut(s) 689
Csp6I GTAC 4 cut(s) 181, 283, 466, 741
CviQI GTAC 4 cut(s) 181, 283, 466, 741
DdeI CTNAG 3 cut(s) 365, 563, 599
DinI GGCGCC 1 cut(s) 438
DpnI GATC 3 cut(s) 275, 491, 512
DpnII GATC 3 cut(s) 273, 489, 510
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 2 cut(s) 299, 368
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 2 cut(s) 67, 261
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 542, 671
Eco88I CYCGRG 4 cut(s) 126, 156, 378, 604
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 222, 476
EcoRV GATATC 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 96
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
FaiI YATR 1 cut(s) 356
FaqI GGGAC 2 cut(s) 494, 692
FauI CCCGC 1 cut(s) 339
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 8 cut(s) 22, 344, 435, 440, 458, 461, 715, 718
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 8 cut(s) 22, 344, 435, 440, 458, 461, 715, 718
FspBI CTAG 1 cut(s) 744
GlaI GCGC 5 cut(s) 392, 394, 396, 438, 639
GluI GCNGC 8 cut(s) 22, 344, 435, 440, 458, 461, 715, 718
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 2 cut(s) 125, 476
HgaI GACGC 1 cut(s) 689
HhaI GCGC 5 cut(s) 393, 395, 397, 439, 640
Hin1I GRCGYC 2 cut(s) 437, 681
Hin6I GCGC 5 cut(s) 391, 393, 395, 437, 638
HinP1I GCGC 5 cut(s) 391, 393, 395, 437, 638
HincII GTYRAC 2 cut(s) 6, 451
HindII GTYRAC 2 cut(s) 6, 451
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 5 cut(s) 317, 418, 515, 536, 727
HphI GGTGA 4 cut(s) 520, 559, 589, 628
Hpy166II GTNNAC 2 cut(s) 6, 451
Hpy188III TCNNGA 5 cut(s) 143, 198, 212, 304, 422
Hpy8I GTNNAC 2 cut(s) 6, 451
Hpy99I CGWCG 6 cut(s) 92, 122, 191, 452, 455, 574
HpyAV CCTTC 2 cut(s) 149, 702
HpyCH4IV ACGT 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 70, 646, 711
HpyF3I CTNAG 3 cut(s) 365, 563, 599
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 2 cut(s) 437, 681
HspAI GCGC 5 cut(s) 391, 393, 395, 437, 638
KasI GGCGCC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 303
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
Kzo9I GATC 3 cut(s) 273, 489, 510
LmnI GCTCC 3 cut(s) 99, 388, 711
Lsp1109I GCAGC 2 cut(s) 355, 444
MaeI CTAG 1 cut(s) 744
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 310, 577
MalI GATC 3 cut(s) 275, 491, 512
MbiI CCGCTC 2 cut(s) 705, 714
MboI GATC 3 cut(s) 273, 489, 510
MboII GAAGA 3 cut(s) 43, 146, 664
MfeI CAATTG 1 cut(s) 732
MhlI GDGCHC 2 cut(s) 96, 327
MluCI AATT 2 cut(s) 698, 732
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 3 cut(s) 326, 545, 736
MmeI TCCRAC 1 cut(s) 651
MroI TCCGGA 1 cut(s) 303
MroNI GCCGGC 1 cut(s) 526
MroXI GAANNNNTTC 2 cut(s) 38, 138
MslI CAYNNNNRTG 3 cut(s) 41, 213, 506
MspA1I CMGCKG 3 cut(s) 21, 346, 649
MspR9I CCNGG 9 cut(s) 157, 158, 224, 280, 291, 361, 379, 380, 478
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 2 cut(s) 47, 556
MvaI CCWGG 2 cut(s) 224, 478
MvnI CGCG 4 cut(s) 199, 393, 395, 506
MwoI GCNNNNNNNGC 4 cut(s) 18, 70, 646, 711
NaeI GCCGGC 1 cut(s) 528
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 7 cut(s) 157, 158, 280, 291, 361, 379, 380
NdeII GATC 3 cut(s) 273, 489, 510
NgoMIV GCCGGC 1 cut(s) 526
NlaIV GGNNCC 3 cut(s) 263, 324, 438
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 310, 577
NruI TCGCGA 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 604
PauI GCGCGC 2 cut(s) 391, 393
PcsI WCGNNNNNNNCGW 3 cut(s) 489, 510, 578
PctI GAATGC 2 cut(s) 47, 556
PdiI GCCGGC 1 cut(s) 528
PdmI GAANNNNTTC 2 cut(s) 38, 138
PfeI GAWTC 2 cut(s) 418, 515
PflFI GACNNNGTC 1 cut(s) 316
PkrI GCNGC 8 cut(s) 23, 345, 436, 441, 459, 462, 716, 719
PleI GAGTC 3 cut(s) 325, 544, 735
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 3 cut(s) 325, 544, 735
PpuMI RGGWCCY 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 222, 476
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 2 cut(s) 222, 476
PspN4I GGNNCC 3 cut(s) 263, 324, 438
PspPI GGNCC 4 cut(s) 67, 124, 261, 474
PspPPI RGGWCCY 1 cut(s) 261
PspXI VCTCGAGB 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 316
PteI GCGCGC 2 cut(s) 391, 393
PvuII CAGCTG 1 cut(s) 649
RruI TCGCGA 1 cut(s) 199
RsaI GTAC 4 cut(s) 182, 284, 467, 742
RsaNI GTAC 4 cut(s) 181, 283, 466, 741
RseI CAYNNNNRTG 3 cut(s) 41, 213, 506
SacI GAGCTC 1 cut(s) 96
SalI GTCGAC 1 cut(s) 449
SatI GCNGC 8 cut(s) 22, 344, 435, 440, 458, 461, 715, 718
Sau3AI GATC 3 cut(s) 273, 489, 510
Sau96I GGNCC 4 cut(s) 67, 124, 261, 474
ScaI AGTACT 2 cut(s) 182, 742
SchI GAGTC 3 cut(s) 326, 545, 736
ScrFI CCNGG 9 cut(s) 157, 158, 224, 280, 291, 361, 379, 380, 478
SduI GDGCHC 2 cut(s) 96, 327
SetI ASST 9 cut(s) 96, 141, 411, 456, 588, 605, 623, 651, 752
SfoI GGCGCC 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 604
SgrDI CGTCGACG 1 cut(s) 449
SinI GGWCC 2 cut(s) 67, 261
SlaI CTCGAG 1 cut(s) 604
SmaI CCCGGG 2 cut(s) 158, 380
SmiMI CAYNNNNRTG 3 cut(s) 41, 213, 506
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
SpeI ACTAGT 1 cut(s) 743
Sse9I AATT 2 cut(s) 698, 732
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 1 cut(s) 744
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 9 cut(s) 155, 156, 222, 278, 289, 359, 377, 378, 476
TaiI ACGT 1 cut(s) 456
TasI AATT 2 cut(s) 698, 732
TatI WGTACW 2 cut(s) 180, 740
TauI GCSGC 6 cut(s) 24, 437, 442, 463, 717, 720
TfiI GAWTC 2 cut(s) 418, 515
TseFI GTSAC 2 cut(s) 310, 577
TseI GCWGC 2 cut(s) 343, 457
Tsp45I GTSAC 2 cut(s) 310, 577
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 2 cut(s) 329, 529
TspMI CCCGGG 2 cut(s) 156, 378
Tth111I GACNNNGTC 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 67, 261
XagI CCTNNNNNAGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 604
XmaI CCCGGG 2 cut(s) 156, 378
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 2 cut(s) 38, 138
XspI CTAG 1 cut(s) 744
ZrmI AGTACT 2 cut(s) 182, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.