MD12G1255900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002273
Physical Location & Seq
Forward (+)
32363508 .. 32364017
510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1255900.v1.1.491

Sequence Viewer

Length: 510 bp
ATGAAGAACAACGAAGATCCCCAATCGCAATCGACGAAGAAAACCAGAGAGTTACCGAATCTGTTGGAGTGCCATTGTTGCCACCTGAGAGTTGACATTGCCAACGCCAGCGCCAAGAGCAAGCTTCAGATCCTCTACAGCGAGTGGCGCGTCGTCCTTCTCTGCAAGAAATGCCTCACGCGCGTCGAGTCCTCGGAGCTCTGCTCCTACTGCTTCGCCGCCACTTCGCCGTCGCAGGAGGACTCTTTCACTTGCTGCCAGTGTAATCGGAGAGTGCACAGGCGTTGCGATTCCGAGTATCGAGGGATTGCGCTGTTGTCTCAGAATTCTTGCTTGGCATTGGAGGCTGAGGTTTGTGCCGATTGCTGGCTGCCGGAGTCGCTTGCGCGGTGGAGAGGGGTTGTGAGGAGTCAGAAGGTGAGGAGTGGAAAGGGGAGAGCTTGTTTGGGATTGGGGAAGTATAGGGTTTTCGCCCGTGGTGATCCAGGGAAAATCATTATCTTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.02

Weight (kDa)

9.01

Isoelectric Point (pI)

68.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 150, 181, 183, 388
AciI CCGC 2 cut(s) 219, 388
AclWI GGATC 3 cut(s) 11, 124, 476
AcsI RAATTY 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 366
AjnI CCWGG 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 124, 199, 440
AluI AGCT 3 cut(s) 124, 199, 440
Alw21I GWGCWC 2 cut(s) 201, 279
Alw26I GTCTC 1 cut(s) 324
Alw44I GTGCAC 1 cut(s) 275
AlwI GGATC 3 cut(s) 11, 124, 476
ApaLI GTGCAC 1 cut(s) 275
ApeKI GCWGC 2 cut(s) 255, 370
ApoI RAATTY 1 cut(s) 325
AspLEI GCGC 5 cut(s) 113, 150, 183, 313, 388
AsuHPI GGTGA 2 cut(s) 430, 491
BaeGI GKGCMC 1 cut(s) 279
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 279
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 242, 357
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 1 cut(s) 508
BfmI CTRYAG 1 cut(s) 136
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 3 cut(s) 219, 256, 371
BlsI GCNGC 3 cut(s) 220, 257, 372
Bme1390I CCNGG 1 cut(s) 486
BmrFI CCNGG 1 cut(s) 486
BplI GAGNNNNNCTC 2 cut(s) 188, 220
Bpu10I CCTNAGC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 192, 475, 485
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 3 cut(s) 192, 475, 485
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 3 cut(s) 77, 335, 339
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 2 cut(s) 421, 436
BseSI GKGCMC 1 cut(s) 279
BseXI GCAGC 2 cut(s) 242, 357
Bsh1236I CGCG 4 cut(s) 150, 181, 183, 388
BsiHKAI GWGCWC 2 cut(s) 201, 279
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 201, 279
Bsp143I GATC 3 cut(s) 16, 129, 481
BspACI CCGC 2 cut(s) 219, 388
BspCNI CTCAG 3 cut(s) 78, 334, 340
BspFNI CGCG 4 cut(s) 150, 181, 183, 388
BspPI GGATC 3 cut(s) 11, 124, 476
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 192, 475, 485
BssMI GATC 3 cut(s) 16, 129, 481
Bst2UI CCWGG 1 cut(s) 486
BstAPI GCANNNNNTGC 1 cut(s) 171
BstC8I GCNNGC 4 cut(s) 109, 122, 368, 384
BstDEI CTNAG 3 cut(s) 86, 321, 348
BstDSI CCRYGG 1 cut(s) 475
BstFNI CGCG 4 cut(s) 150, 181, 183, 388
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 5 cut(s) 113, 150, 183, 313, 388
BstKTI GATC 3 cut(s) 19, 132, 484
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 3 cut(s) 16, 129, 481
BstMWI GCNNNNNNNGC 8 cut(s) 78, 117, 147, 171, 180, 210, 344, 379
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 136
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 4 cut(s) 150, 181, 183, 388
BstV1I GCAGC 2 cut(s) 242, 357
BstX2I RGATCY 2 cut(s) 16, 129
BstYI RGATCY 2 cut(s) 16, 129
BtgI CCRYGG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 266
Cac8I GCNNGC 4 cut(s) 109, 122, 368, 384
CfoI GCGC 5 cut(s) 113, 150, 183, 313, 388
CseI GACGC 2 cut(s) 139, 172
CviJI RGCY 6 cut(s) 124, 199, 347, 370, 440, 507
CviKI_1 RGCY 6 cut(s) 124, 199, 347, 370, 440, 507
DdeI CTNAG 3 cut(s) 86, 321, 348
DpnI GATC 3 cut(s) 18, 131, 483
DpnII GATC 3 cut(s) 16, 129, 481
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 201
FaiI YATR 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 219, 256, 371
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 219, 256, 371
FspBI CTAG 1 cut(s) 508
GlaI GCGC 5 cut(s) 112, 149, 182, 312, 387
GluI GCNGC 3 cut(s) 219, 256, 371
HaeII RGCGCY 1 cut(s) 114
HapII CCGG 1 cut(s) 374
HgaI GACGC 2 cut(s) 139, 172
HhaI GCGC 5 cut(s) 113, 150, 183, 313, 388
Hin6I GCGC 5 cut(s) 111, 148, 181, 311, 386
HinP1I GCGC 5 cut(s) 111, 148, 181, 311, 386
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 6 cut(s) 58, 188, 242, 290, 377, 409
HpaII CCGG 1 cut(s) 374
HphI GGTGA 2 cut(s) 430, 491
Hpy166II GTNNAC 2 cut(s) 94, 277
Hpy188I TCNGA 6 cut(s) 129, 196, 270, 295, 324, 414
Hpy8I GTNNAC 2 cut(s) 94, 277
Hpy99I CGWCG 4 cut(s) 37, 155, 188, 235
HpyAV CCTTC 2 cut(s) 167, 409
HpyCH4V TGCA 2 cut(s) 165, 277
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 117, 147, 171, 180, 210, 344, 379
HpyF3I CTNAG 3 cut(s) 86, 321, 348
HspAI GCGC 5 cut(s) 111, 148, 181, 311, 386
Kzo9I GATC 3 cut(s) 16, 129, 481
LmnI GCTCC 2 cut(s) 196, 209
Lsp1109I GCAGC 2 cut(s) 242, 357
MaeI CTAG 1 cut(s) 508
MaeIII GTNAC 1 cut(s) 51
MalI GATC 3 cut(s) 18, 131, 483
MboI GATC 3 cut(s) 16, 129, 481
MboII GAAGA 3 cut(s) 16, 26, 49
MflI RGATCY 2 cut(s) 16, 129
MhlI GDGCHC 2 cut(s) 201, 279
MluCI AATT 1 cut(s) 325
MlyI GAGTC 4 cut(s) 197, 236, 386, 418
MmeI TCCRAC 1 cut(s) 45
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 486
MvnI CGCG 4 cut(s) 150, 181, 183, 388
MwoI GCNNNNNNNGC 8 cut(s) 78, 117, 147, 171, 180, 210, 344, 379
NdeII GATC 3 cut(s) 16, 129, 481
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PfeI GAWTC 2 cut(s) 58, 290
PkrI GCNGC 3 cut(s) 220, 257, 372
PleI GAGTC 4 cut(s) 196, 236, 385, 417
PpsI GAGTC 4 cut(s) 196, 236, 385, 417
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PsuI RGATCY 2 cut(s) 16, 129
SacI GAGCTC 1 cut(s) 201
SatI GCNGC 3 cut(s) 219, 256, 371
Sau3AI GATC 3 cut(s) 16, 129, 481
SchI GAGTC 4 cut(s) 197, 236, 386, 418
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 201, 279
SetI ASST 6 cut(s) 87, 126, 201, 354, 420, 442
SfcI CTRYAG 1 cut(s) 136
Sse9I AATT 1 cut(s) 325
SsiI CCGC 2 cut(s) 219, 388
SspMI CTAG 1 cut(s) 508
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 484
TaqI TCGA 3 cut(s) 32, 186, 301
TasI AATT 1 cut(s) 325
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 58, 290
TscAI CASTG 1 cut(s) 266
TseI GCWGC 2 cut(s) 255, 370
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 275
XapI RAATTY 1 cut(s) 325
XspI CTAG 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.