Rorug02G0614000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002273
Physical Location & Seq
Forward (+)
73176762 .. 73177545
784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0614000.1

Sequence Viewer

Length: 540 bp
ATGGCTGCAGCTCCAAGTGATTCATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAATTGCAGATGTTCTGAAATTTGATCCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTAGTTGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTGGTTTCTTCAAGGACATAGAGGGTGATAGTCCCTTACCTATTTTTCTACTACATCAGAAGAAGTTACCTTTGTTTATTTGTTCATTTTCAATTTGGTTTGCAGACAATTCCAGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCTAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAATAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACTGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAATCTGAGCTTTGTTGAAGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.65

Weight (kDa)

5.36

Isoelectric Point (pI)

26.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 98 - 170 1.3e-06 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 74, 426
AcsI RAATTY 1 cut(s) 74
AfaI GTAC 1 cut(s) 473
AgsI TTSAA 3 cut(s) 184, 264, 530
AjnI CCWGG 2 cut(s) 114, 284
AluBI AGCT 3 cut(s) 11, 170, 522
AluI AGCT 3 cut(s) 11, 170, 522
Alw21I GWGCWC 1 cut(s) 172
AlwI GGATC 2 cut(s) 74, 426
AlwNI CAGNNNCTG 1 cut(s) 121
ApeKI GCWGC 4 cut(s) 5, 8, 137, 479
ApoI RAATTY 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 209, 322, 451
AvaII GGWCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 3 cut(s) 20, 124, 466
BciT130I CCWGG 2 cut(s) 116, 286
BclI TGATCA 1 cut(s) 123
BfaI CTAG 2 cut(s) 326, 538
BfmI CTRYAG 2 cut(s) 6, 480
BisI GCNGC 4 cut(s) 6, 9, 138, 480
BlsI GCNGC 4 cut(s) 7, 10, 139, 481
Bme1390I CCNGG 2 cut(s) 116, 286
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmrFI CCNGG 2 cut(s) 116, 286
BsaJI CCNNGG 1 cut(s) 412
BseBI CCWGG 2 cut(s) 116, 286
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 509
BseXI GCAGC 3 cut(s) 20, 124, 466
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 172
BslFI GGGAC 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 189
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 79, 123, 418
Bsp19I CCATGG 1 cut(s) 412
BspCNI CTCAG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 162
BspMAI CTGCAG 2 cut(s) 10, 484
BspPI GGATC 2 cut(s) 74, 426
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 3 cut(s) 79, 123, 418
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 2 cut(s) 116, 286
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 1 cut(s) 518
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 3 cut(s) 82, 126, 421
BstMBI GATC 3 cut(s) 79, 123, 418
BstNI CCWGG 2 cut(s) 116, 286
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 2 cut(s) 114, 284
BstSFI CTRYAG 2 cut(s) 6, 480
BstV1I GCAGC 3 cut(s) 20, 124, 466
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 535
CaiI CAGNNNCTG 1 cut(s) 121
CciI TCATGA 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 114
Csp6I GTAC 1 cut(s) 472
CviAII CATG 5 cut(s) 163, 398, 413, 493, 534
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 1 cut(s) 518
DpnI GATC 3 cut(s) 81, 125, 420
DpnII GATC 3 cut(s) 79, 123, 418
Ecl136II GAGCTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 114, 284
EcoT14I CCWWGG 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 5 cut(s) 166, 401, 416, 496, 537
FaqI GGGAC 1 cut(s) 189
FatI CATG 5 cut(s) 162, 397, 412, 492, 533
FauNDI CATATG 1 cut(s) 25
FbaI TGATCA 1 cut(s) 123
Fnu4HI GCNGC 4 cut(s) 6, 9, 138, 480
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 4 cut(s) 6, 9, 138, 480
FspBI CTAG 2 cut(s) 326, 538
GluI GCNGC 4 cut(s) 6, 9, 138, 480
GsaI CCCAGC 1 cut(s) 458
Hin1II CATG 5 cut(s) 166, 401, 416, 496, 537
HinfI GANTC 2 cut(s) 20, 385
HphI GGTGA 3 cut(s) 209, 322, 451
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 4 cut(s) 46, 72, 231, 519
Hpy188III TCNNGA 2 cut(s) 98, 163
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 1 cut(s) 307
HpyCH4V TGCA 5 cut(s) 8, 62, 275, 331, 482
HpyF3I CTNAG 1 cut(s) 518
Hsp92II CATG 5 cut(s) 166, 401, 416, 496, 537
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 3 cut(s) 79, 123, 418
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 9 cut(s) 101, 111, 128, 271, 285, 298, 436, 440, 468
Lsp1109I GCAGC 3 cut(s) 20, 124, 466
MabI ACCWGGT 1 cut(s) 114
MaeI CTAG 2 cut(s) 326, 538
MaeIII GTNAC 1 cut(s) 237
MalI GATC 3 cut(s) 81, 125, 420
MboI GATC 3 cut(s) 79, 123, 418
MboII GAAGA 2 cut(s) 172, 244
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 6 cut(s) 57, 74, 102, 153, 264, 280
MnlI CCTC 2 cut(s) 103, 187
MseI TTAA 3 cut(s) 105, 147, 339
MspR9I CCNGG 2 cut(s) 116, 286
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 2 cut(s) 116, 286
NcoI CCATGG 1 cut(s) 412
NdeI CATATG 1 cut(s) 25
NdeII GATC 3 cut(s) 79, 123, 418
NlaIII CATG 5 cut(s) 166, 401, 416, 496, 537
NspI RCATGY 1 cut(s) 537
PaeI GCATGC 1 cut(s) 537
PagI TCATGA 1 cut(s) 162
PfeI GAWTC 2 cut(s) 20, 385
PkrI GCNGC 4 cut(s) 7, 10, 139, 481
Psp124BI GAGCTC 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 114, 284
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 2 cut(s) 114, 284
PspPI GGNCC 1 cut(s) 112
PstI CTGCAG 2 cut(s) 10, 484
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 3 cut(s) 105, 147, 339
SatI GCNGC 4 cut(s) 6, 9, 138, 480
Sau3AI GATC 3 cut(s) 79, 123, 418
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 116, 286
SduI GDGCHC 1 cut(s) 172
SexAI ACCWGGT 1 cut(s) 114
SfcI CTRYAG 2 cut(s) 6, 480
SinI GGWCC 1 cut(s) 112
SphI GCATGC 1 cut(s) 537
Sse9I AATT 6 cut(s) 57, 74, 102, 153, 264, 280
SspMI CTAG 2 cut(s) 326, 538
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 114, 284
StyI CCWWGG 1 cut(s) 412
TasI AATT 6 cut(s) 57, 74, 102, 153, 264, 280
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 2 cut(s) 20, 385
Tru1I TTAA 3 cut(s) 105, 147, 339
Tru9I TTAA 3 cut(s) 105, 147, 339
TscAI CASTG 1 cut(s) 481
TseI GCWGC 4 cut(s) 5, 8, 137, 479
TspDTI ATGAA 4 cut(s) 12, 151, 246, 366
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 74
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XspI CTAG 2 cut(s) 326, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.