RLG00000025884

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002273
Physical Location & Seq
Forward (+)
48927598 .. 48930618
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025884

Sequence Viewer

Length: 1386 bp
ATGACGACGACGAGCAGTGAGAAGAAGAAGAAGACTCGGGAGTTGCCGAACCTGACGGAGTGCCATTGCTGCCACCTCAGAGTCGACCTCGCCAAATCCTCCAAGAACAACAAGCTTCAGATTCTCTACAGCGAGTGGCGGATCGTCCTCCTCTGCAACAAGTGCTTGGCCCGCGTCGAATCCACCGAGCTCTGCTCCTACTGCTTCAACGACTCGTTGTCCGGCCACTGCTTCTCTTGCCGCCAGTGCAGGCGGCGAGTCCACAGGGACTGCGATTCCGAGTACCGCAGCGCCGCCGTCTGGTCCGGGTCGTCGTCTGCCCCCGCCGACGAGGTCTCTGTCTGCGCCGATTGCTGGGTGTCGGAGTCGCTGGCCAGGTGGAGGAGAAGTAGAAACGTCAGTAGTGGTAGAAAGAGTAGGGCGGATTTGGGATTGGGGAGATCTAGGGTTTTGAAGTATGAGAGGAAATGTGATCCAAATTCGACTGATTCATCTACAGAAGAGGAGGCCGGGTGTTTGAGACTTGATGGTGTTGAAGAAGAAGAAGAGATTGGGGATGATGAGCCGTTGAAGGAAGGACAAGGGAGTTGCTCAAATGGTGTCGAATCGGAGTCTCCACCGTCTTATAAACAAGATGACTGCAACTGTATCGCTTTGCCGAAAGTCGAAATTGGGAATGCAAAGCTTGATCCTTATTATTTCAAGTACAGGAGGAGGAGGAGCAGTGTTGATCACTCTCTGTTGACATACAAGAGGAGGAGCATTGTTGACTGCTTTAAGATTGATTGTCCTCTGTTGACGTATAAGAGGAGAGGCAGTGTTGACTATCGGTTTATGTTTACGTATAAGAGGAGGAGGAACTTTGATCAATCTATGTTGACATATAAGAGGAGGAGACCTGAAATCGTTTACGCGAGTTCAAAGATGCAGCTCGAGGGGGAAGTAACTTCAATTCATGATAAGATTCTAGACTATGTTAAAGATCCTTTTCACTTGGCATGTTTCCAAAGAAGAAACATTGGGCTTGATGGACTGTTGAAGGATGGAAGTTGTACAAATGGTGTTGACTTGGAGTCTCCACCTTATAAACAAGAACACTGCATGTGTATCACCAGGCCAAAGGAACGAATAATGCAATGCAAAGTTCAACCTATTTTTCTAAGTACAGGAGAAGGAGCACTATTGATCACTTTGTTAACATACAAGAGGAGGAGCAGTTTTCATCGCTGTAAGAATTTGAGCACCATTGATTGCCCTATGTTGACATATAGCACCATCGGTTGCTCTTTGTTAACATATGAGCGGAGGATAGCTGAAATCCTCTACACTAGTTCGAGATTAAGTTTGGTTGAAGGTAGCAGCATCACAAACTTTGATGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

52.9

Weight (kDa)

8.71

Isoelectric Point (pI)

74.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 627, 1086
AccBSI CCGCTC 1 cut(s) 1303
AccI GTMKAC 1 cut(s) 84
AccII CGCG 2 cut(s) 174, 914
AciI CCGC 9 cut(s) 139, 172, 241, 253, 286, 294, 324, 422, 1303
AclWI GGATC 4 cut(s) 149, 467, 683, 977
AcoI YGGCCR 2 cut(s) 223, 372
AcsI RAATTY 2 cut(s) 478, 1234
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 4 cut(s) 284, 707, 1054, 1165
AfiI CCNNNNNNNGG 3 cut(s) 300, 354, 381
AhdI GACNNNNNGTC 2 cut(s) 217, 1072
AhlI ACTAGT 1 cut(s) 1328
AjnI CCWGG 2 cut(s) 374, 1112
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AluBI AGCT 5 cut(s) 115, 190, 685, 931, 1313
AluI AGCT 5 cut(s) 115, 190, 685, 931, 1313
Alw21I GWGCWC 3 cut(s) 192, 1180, 1244
Alw26I GTCTC 5 cut(s) 340, 514, 618, 889, 1080
AlwI GGATC 4 cut(s) 149, 467, 683, 977
AlwNI CAGNNNCTG 1 cut(s) 270
Ama87I CYCGRG 2 cut(s) 36, 932
AoxI GGCC 5 cut(s) 168, 223, 372, 507, 1115
ApeKI GCWGC 4 cut(s) 69, 288, 928, 1359
ApoI RAATTY 2 cut(s) 478, 1234
AspLEI GCGC 2 cut(s) 293, 347
AspS9I GGNCC 2 cut(s) 169, 303
AsuC2I CCSGG 2 cut(s) 307, 511
AsuHPI GGTGA 1 cut(s) 1102
AvaI CYCGRG 2 cut(s) 36, 932
AvaII GGWCC 1 cut(s) 303
BalI TGGCCA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 3 cut(s) 192, 1180, 1244
BbvI GCAGC 4 cut(s) 56, 300, 940, 1371
BccI CCATC 4 cut(s) 521, 1022, 1037, 1283
BceAI ACGGC 2 cut(s) 281, 550
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BciT130I CCWGG 2 cut(s) 376, 1114
BclI TGATCA 3 cut(s) 730, 865, 1185
BcnI CCSGG 2 cut(s) 307, 511
BcoDI GTCTC 5 cut(s) 340, 514, 618, 889, 1080
BcuI ACTAGT 1 cut(s) 1328
BfaI CTAG 3 cut(s) 444, 968, 1329
BfmI CTRYAG 2 cut(s) 127, 495
BfoI RGCGCY 1 cut(s) 294
BglII AGATCT 1 cut(s) 440
BisI GCNGC 7 cut(s) 70, 241, 254, 289, 294, 929, 1360
BlsI GCNGC 7 cut(s) 71, 242, 255, 290, 295, 930, 1361
Bme1390I CCNGG 4 cut(s) 307, 376, 511, 1114
Bme18I GGWCC 1 cut(s) 303
BmeRI GACNNNNNGTC 2 cut(s) 217, 1072
BmeT110I CYCGRG 2 cut(s) 36, 932
BmgT120I GGNCC 2 cut(s) 169, 303
BmrFI CCNGG 4 cut(s) 307, 376, 511, 1114
BmsI GCATC 2 cut(s) 915, 1371
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 4 cut(s) 72, 104, 179, 211
BpuMI CCSGG 2 cut(s) 307, 511
BsaAI YACGTR 1 cut(s) 843
BsaI GGTCTC 2 cut(s) 340, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 354, 381
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 2 cut(s) 64, 1142
BseBI CCWGG 2 cut(s) 376, 1114
BseGI GGATG 2 cut(s) 562, 1048
BseLI CCNNNNNNNGG 3 cut(s) 300, 354, 381
BseMI GCAATG 2 cut(s) 64, 1142
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 1 cut(s) 244
BseXI GCAGC 4 cut(s) 56, 300, 940, 1371
BseYI CCCAGC 1 cut(s) 354
BsgI GTGCAG 1 cut(s) 268
Bsh1236I CGCG 2 cut(s) 174, 914
BshFI GGCC 5 cut(s) 170, 225, 374, 509, 1117
BsiHKAI GWGCWC 3 cut(s) 192, 1180, 1244
BsiHKCI CYCGRG 2 cut(s) 36, 932
BsiSI CCGG 3 cut(s) 222, 306, 510
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 3 cut(s) 300, 354, 381
BsmAI GTCTC 5 cut(s) 340, 514, 618, 889, 1080
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 5 cut(s) 170, 225, 374, 509, 1117
Bso31I GGTCTC 2 cut(s) 340, 889
BsoBI CYCGRG 2 cut(s) 36, 932
Bsp1286I GDGCHC 3 cut(s) 192, 1180, 1244
Bsp1407I TGTACA 1 cut(s) 1052
Bsp143I GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
BspACI CCGC 9 cut(s) 139, 172, 241, 253, 286, 294, 324, 422, 1303
BspANI GGCC 5 cut(s) 170, 225, 374, 509, 1117
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 2 cut(s) 174, 914
BspHI TCATGA 1 cut(s) 955
BspPI GGATC 4 cut(s) 149, 467, 683, 977
BspTNI GGTCTC 2 cut(s) 340, 889
BsrBI CCGCTC 1 cut(s) 1303
BsrDI GCAATG 2 cut(s) 64, 1142
BsrGI TGTACA 1 cut(s) 1052
BsrI ACTGG 1 cut(s) 244
BssMI GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
Bst2UI CCWGG 2 cut(s) 376, 1114
Bst4CI ACNGT 3 cut(s) 621, 647, 1035
Bst6I CTCTTC 2 cut(s) 495, 540
BstAPI GCANNNNNTGC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 1052
BstBAI YACGTR 1 cut(s) 843
BstC8I GCNNGC 3 cut(s) 172, 251, 372
BstDEI CTNAG 2 cut(s) 77, 1160
BstF5I GGATG 2 cut(s) 562, 1048
BstFNI CGCG 2 cut(s) 174, 914
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 2 cut(s) 293, 347
BstKTI GATC 8 cut(s) 144, 443, 475, 691, 733, 868, 985, 1188
BstMAI GTCTC 5 cut(s) 340, 514, 618, 889, 1080
BstMBI GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
BstMWI GCNNNNNNNGC 7 cut(s) 69, 162, 171, 201, 237, 246, 351
BstNI CCWGG 2 cut(s) 376, 1114
BstNSI RCATGY 2 cut(s) 1002, 1105
BstSCI CCNGG 4 cut(s) 305, 374, 509, 1112
BstSFI CTRYAG 2 cut(s) 127, 495
BstSNI TACGTA 1 cut(s) 843
BstUI CGCG 2 cut(s) 174, 914
BstV1I GCAGC 4 cut(s) 56, 300, 940, 1371
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 440, 982
BstYI RGATCY 2 cut(s) 440, 982
BsuRI GGCC 5 cut(s) 170, 225, 374, 509, 1117
BtgZI GCGATG 1 cut(s) 1208
BtsCI GGATG 2 cut(s) 562, 1048
BtsI GCAGTG 5 cut(s) 22, 226, 730, 823, 1096
BtsIMutI CAGTG 6 cut(s) 22, 226, 251, 730, 823, 1096
Cac8I GCNNGC 3 cut(s) 172, 251, 372
CaiI CAGNNNCTG 1 cut(s) 270
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 293, 347
Cfr13I GGNCC 2 cut(s) 169, 303
CseI GACGC 1 cut(s) 163
Csp6I GTAC 4 cut(s) 283, 706, 1053, 1164
CviAII CATG 3 cut(s) 956, 999, 1102
CviQI GTAC 4 cut(s) 283, 706, 1053, 1164
DdeI CTNAG 2 cut(s) 77, 1160
DpnI GATC 8 cut(s) 143, 442, 474, 690, 732, 867, 984, 1187
DpnII GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
DriI GACNNNNNGTC 2 cut(s) 217, 1072
EaeI YGGCCR 2 cut(s) 223, 372
Eam1104I CTCTTC 2 cut(s) 495, 540
Eam1105I GACNNNNNGTC 2 cut(s) 217, 1072
EarI CTCTTC 2 cut(s) 495, 540
EciI GGCGGA 2 cut(s) 154, 437
Ecl136II GAGCTC 1 cut(s) 190
Eco105I TACGTA 1 cut(s) 843
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 2 cut(s) 340, 889
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 101
Eco88I CYCGRG 2 cut(s) 36, 932
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 374, 1112
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 3 cut(s) 959, 1002, 1105
FaqI GGGAC 1 cut(s) 281
FatI CATG 3 cut(s) 955, 998, 1101
FauI CCCGC 2 cut(s) 179, 331
FauNDI CATATG 1 cut(s) 1297
FbaI TGATCA 3 cut(s) 730, 865, 1185
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 7 cut(s) 70, 241, 254, 289, 294, 929, 1360
FokI GGATG 2 cut(s) 569, 1055
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 7 cut(s) 70, 241, 254, 289, 294, 929, 1360
FspBI CTAG 3 cut(s) 444, 968, 1329
GlaI GCGC 2 cut(s) 292, 346
GluI GCNGC 7 cut(s) 70, 241, 254, 289, 294, 929, 1360
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 5 cut(s) 170, 225, 374, 509, 1117
HapII CCGG 3 cut(s) 222, 306, 510
HgaI GACGC 1 cut(s) 163
HhaI GCGC 2 cut(s) 293, 347
Hin1II CATG 3 cut(s) 959, 1002, 1105
Hin6I GCGC 2 cut(s) 291, 345
HinP1I GCGC 2 cut(s) 291, 345
HindIII AAGCTT 2 cut(s) 113, 683
HpaI GTTAAC 2 cut(s) 1197, 1293
HpaII CCGG 3 cut(s) 222, 306, 510
HphI GGTGA 1 cut(s) 1102
Hpy188I TCNGA 5 cut(s) 80, 120, 280, 364, 610
Hpy188III TCNNGA 4 cut(s) 38, 956, 968, 1335
Hpy99I CGWCG 5 cut(s) 10, 13, 179, 316, 332
HpyAV CCTTC 5 cut(s) 565, 569, 1033, 1166, 1346
HpyCH4III ACNGT 3 cut(s) 621, 647, 1035
HpyCH4IV ACGT 3 cut(s) 396, 800, 842
HpyCH4V TGCA 8 cut(s) 156, 249, 642, 680, 928, 1101, 1135, 1140
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 162, 171, 201, 237, 246, 351
HpyF3I CTNAG 2 cut(s) 77, 1160
HpySE526I ACGT 3 cut(s) 396, 800, 842
Hsp92II CATG 3 cut(s) 959, 1002, 1105
HspAI GCGC 2 cut(s) 291, 345
Ksp22I TGATCA 3 cut(s) 730, 865, 1185
KspAI GTTAAC 2 cut(s) 1197, 1293
Kzo9I GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
LmnI GCTCC 5 cut(s) 200, 720, 759, 1175, 1212
Lsp1109I GCAGC 4 cut(s) 56, 300, 940, 1371
LweI GCATC 2 cut(s) 915, 1371
MaeI CTAG 3 cut(s) 444, 968, 1329
MaeII ACGT 3 cut(s) 396, 800, 842
MaeIII GTNAC 1 cut(s) 943
MalI GATC 8 cut(s) 143, 442, 474, 690, 732, 867, 984, 1187
MbiI CCGCTC 1 cut(s) 1303
MboI GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
MflI RGATCY 2 cut(s) 440, 982
MhlI GDGCHC 3 cut(s) 192, 1180, 1244
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 4 cut(s) 478, 669, 951, 1234
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 7 cut(s) 28, 90, 206, 267, 374, 620, 1082
MmeI TCCRAC 1 cut(s) 342
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
MseI TTAA 5 cut(s) 777, 978, 1196, 1292, 1340
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 3 cut(s) 222, 306, 510
MspR9I CCNGG 4 cut(s) 307, 376, 511, 1114
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 2 cut(s) 376, 1114
MvnI CGCG 2 cut(s) 174, 914
MwoI GCNNNNNNNGC 7 cut(s) 69, 162, 171, 201, 237, 246, 351
NciI CCSGG 2 cut(s) 307, 511
NdeI CATATG 1 cut(s) 1297
NdeII GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
NlaIII CATG 3 cut(s) 959, 1002, 1105
NspI RCATGY 2 cut(s) 1002, 1105
PaeR7I CTCGAG 1 cut(s) 932
PagI TCATGA 1 cut(s) 955
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 6 cut(s) 121, 179, 275, 488, 605, 964
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 7 cut(s) 71, 242, 255, 290, 295, 930, 1361
PleI GAGTC 7 cut(s) 28, 89, 206, 266, 373, 619, 1081
PpsI GAGTC 7 cut(s) 28, 89, 206, 266, 373, 619, 1081
Ppu21I YACGTR 1 cut(s) 843
PsiI TTATAA 2 cut(s) 627, 1086
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 374, 1112
PspFI CCCAGC 1 cut(s) 354
PspGI CCWGG 2 cut(s) 374, 1112
PspPI GGNCC 2 cut(s) 169, 303
PspXI VCTCGAGB 1 cut(s) 932
PstNI CAGNNNCTG 1 cut(s) 270
PsuI RGATCY 2 cut(s) 440, 982
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 4 cut(s) 284, 707, 1054, 1165
RsaNI GTAC 4 cut(s) 283, 706, 1053, 1164
SacI GAGCTC 1 cut(s) 192
SalI GTCGAC 1 cut(s) 83
SaqAI TTAA 5 cut(s) 777, 978, 1196, 1292, 1340
SatI GCNGC 7 cut(s) 70, 241, 254, 289, 294, 929, 1360
Sau3AI GATC 8 cut(s) 141, 440, 472, 688, 730, 865, 982, 1185
Sau96I GGNCC 2 cut(s) 169, 303
SchI GAGTC 7 cut(s) 28, 90, 206, 267, 374, 620, 1082
ScrFI CCNGG 4 cut(s) 307, 376, 511, 1114
SduI GDGCHC 3 cut(s) 192, 1180, 1244
SfaNI GCATC 2 cut(s) 915, 1371
SfcI CTRYAG 2 cut(s) 127, 495
Sfr274I CTCGAG 1 cut(s) 932
SinI GGWCC 1 cut(s) 303
SlaI CTCGAG 1 cut(s) 932
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
SnaBI TACGTA 1 cut(s) 843
SpeI ACTAGT 1 cut(s) 1328
Sse9I AATT 4 cut(s) 478, 669, 951, 1234
SsiI CCGC 9 cut(s) 139, 172, 241, 253, 286, 294, 324, 422, 1303
SspMI CTAG 3 cut(s) 444, 968, 1329
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 305, 374, 509, 1112
TaaI ACNGT 3 cut(s) 621, 647, 1035
TaiI ACGT 3 cut(s) 399, 803, 845
TaqI TCGA 7 cut(s) 84, 177, 482, 603, 666, 933, 1334
TasI AATT 4 cut(s) 478, 669, 951, 1234
TatI WGTACW 3 cut(s) 705, 1052, 1163
TauI GCSGC 3 cut(s) 243, 256, 296
TfiI GAWTC 6 cut(s) 121, 179, 275, 488, 605, 964
Tru1I TTAA 5 cut(s) 777, 978, 1196, 1292, 1340
Tru9I TTAA 5 cut(s) 777, 978, 1196, 1292, 1340
TscAI CASTG 6 cut(s) 22, 233, 251, 730, 823, 1103
TseI GCWGC 4 cut(s) 69, 288, 928, 1359
TspDTI ATGAA 3 cut(s) 480, 944, 1211
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 6 cut(s) 22, 233, 251, 730, 823, 1103
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 2 cut(s) 478, 1234
XbaI TCTAGA 1 cut(s) 967
XceI RCATGY 2 cut(s) 1002, 1105
XhoI CTCGAG 1 cut(s) 932
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 3 cut(s) 444, 968, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.