Rorug02G0613900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002273
Physical Location & Seq
Forward (+)
73166602 .. 73168795
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0613900.1

Sequence Viewer

Length: 1029 bp
ATGGCTGCAGCTCCAAGTGAGTGCATACCTTCTGTATTTGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAGTGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTAATCAAGGTGGTTGCTGCTTCTCTTAACCCAGTTGATTTCAAAAGGGCTCTTGGTCGCTTCAAGGACATAGAAGGTGATACTATCTTACCTACAATTCCAGGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTACAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCACTGAGATTCCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACGGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAATCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACTGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCAGCTGGTAAATCAGTCCTTGTTTTGGGAGGCGCTGGGGGTGTTGGAACACATGTGATTCAGCTAGCTAAACATGATTTTGGGGCATCCAAAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTAAGAAGCTTGGGTACAGATTTGGCTATTGATTACACCAAGGAAAACATCGAAGACCTGCCAGAGAAATTTGATGTTGTCTATGATGCAGTTGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAAGGTAGTGACAATAGTCATAGGTCCAGTAACTCCTCCGGCGTTCAAATTTAGGTTCACCCCTACAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTGAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCTAGAGCTACAGGAAAGGCCCTGAGGGCTGCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

36.24

Weight (kDa)

6.17

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 153 8.3e-17 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 167 - 276 3.6e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 194 - 278 7.5e-14 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 227 - 336 2.2e-16 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 735
AccBSI CCGCTC 2 cut(s) 533, 909
AciI CCGC 3 cut(s) 533, 788, 909
AclWI GGATC 3 cut(s) 86, 375, 926
AcsI RAATTY 3 cut(s) 86, 737, 851
AcuI CTGAAG 2 cut(s) 911, 978
AfaI GTAC 3 cut(s) 279, 422, 685
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 565, 870
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 6 cut(s) 175, 196, 479, 527, 850, 968
AjnI CCWGG 2 cut(s) 126, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
Alw26I GTCTC 2 cut(s) 500, 980
AlwI GGATC 3 cut(s) 86, 375, 926
AlwNI CAGNNNCTG 1 cut(s) 644
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 1 cut(s) 1008
ApeKI GCWGC 8 cut(s) 5, 8, 149, 428, 482, 638, 1019, 1022
ApoI RAATTY 3 cut(s) 86, 737, 851
AspLEI GCGC 2 cut(s) 523, 575
AspS9I GGNCC 4 cut(s) 124, 827, 941, 1009
AsuHPI GGTGA 6 cut(s) 221, 271, 400, 802, 853, 928
AsuNHI GCTAGC 2 cut(s) 485, 604
AvaII GGWCC 3 cut(s) 124, 827, 941
AxyI CCTNAGG 1 cut(s) 1013
BanII GRGCYC 1 cut(s) 184
BarI GAAGNNNNNNTAC 2 cut(s) 667, 699
BbsI GAAGAC 1 cut(s) 729
BbvI GCAGC 7 cut(s) 20, 136, 415, 469, 650, 1006, 1009
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 2 cut(s) 128, 234
BcoDI GTCTC 2 cut(s) 500, 980
BfaI CTAG 3 cut(s) 486, 605, 993
BfmI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
BfoI RGCGCY 2 cut(s) 524, 576
BfuAI ACCTGC 1 cut(s) 735
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 8 cut(s) 6, 9, 150, 429, 483, 639, 1020, 1023
BlsI GCNGC 8 cut(s) 7, 10, 151, 430, 484, 640, 1021, 1024
Bme1390I CCNGG 2 cut(s) 128, 234
Bme18I GGWCC 3 cut(s) 124, 827, 941
BmgT120I GGNCC 4 cut(s) 124, 827, 941, 1009
BmrFI CCNGG 2 cut(s) 128, 234
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 2 cut(s) 635, 745
BmtI GCTAGC 2 cut(s) 489, 608
BmuI ACTGGG 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 818
BpiI GAAGAC 1 cut(s) 729
Bpu10I CCTNAGC 1 cut(s) 496
BpuEI CTTGAG 1 cut(s) 1004
BsaJI CCNNGG 3 cut(s) 233, 361, 708
Bsc4I CCNNNNNNNGG 2 cut(s) 565, 870
Bse1I ACTGG 4 cut(s) 164, 666, 830, 923
Bse21I CCTNAGG 1 cut(s) 1013
BseBI CCWGG 2 cut(s) 128, 234
BseDI CCNNGG 3 cut(s) 233, 361, 708
BseGI GGATG 2 cut(s) 304, 626
BseLI CCNNNNNNNGG 2 cut(s) 565, 870
BseMII CTCAG 3 cut(s) 321, 458, 1004
BseNI ACTGG 4 cut(s) 164, 666, 830, 923
BseRI GAGGAG 1 cut(s) 828
BseXI GCAGC 7 cut(s) 20, 136, 415, 469, 650, 1006, 1009
BseYI CCCAGC 2 cut(s) 403, 575
BshFI GGCC 1 cut(s) 1010
BsiSI CCGG 1 cut(s) 842
BslI CCNNNNNNNGG 2 cut(s) 565, 870
BsmAI GTCTC 2 cut(s) 500, 980
BsnI GGCC 1 cut(s) 1010
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 3 cut(s) 91, 367, 931
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 3 cut(s) 533, 788, 909
BspANI GGCC 1 cut(s) 1010
BspCNI CTCAG 3 cut(s) 322, 459, 1005
BspMAI CTGCAG 3 cut(s) 10, 76, 433
BspMI ACCTGC 1 cut(s) 735
BspOI GCTAGC 2 cut(s) 489, 608
BspPI GGATC 3 cut(s) 86, 375, 926
BsrBI CCGCTC 2 cut(s) 533, 909
BsrI ACTGG 4 cut(s) 164, 666, 830, 923
BssECI CCNNGG 3 cut(s) 233, 361, 708
BssMI GATC 3 cut(s) 91, 367, 931
BssT1I CCWWGG 2 cut(s) 361, 708
Bst2UI CCWGG 2 cut(s) 128, 234
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 487, 606, 649
BstDEI CTNAG 5 cut(s) 330, 351, 467, 496, 1013
BstDSI CCRYGG 1 cut(s) 361
BstEII GGTNACC 1 cut(s) 977
BstF5I GGATG 2 cut(s) 304, 626
BstH2I RGCGCY 2 cut(s) 524, 576
BstHHI GCGC 2 cut(s) 523, 575
BstKTI GATC 3 cut(s) 94, 370, 934
BstMAI GTCTC 2 cut(s) 500, 980
BstMBI GATC 3 cut(s) 91, 367, 931
BstMWI GCNNNNNNNGC 4 cut(s) 518, 644, 785, 1016
BstNI CCWGG 2 cut(s) 128, 234
BstNSI RCATGY 1 cut(s) 596
BstPAI GACNNNNGTC 1 cut(s) 818
BstPI GGTNACC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 126, 232
BstSFI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
BstV1I GCAGC 7 cut(s) 20, 136, 415, 469, 650, 1006, 1009
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
Bsu36I CCTNAGG 1 cut(s) 1013
BsuRI GGCC 1 cut(s) 1010
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 304, 626
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 3 cut(s) 327, 786, 930
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 487, 606, 649
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 2 cut(s) 523, 575
Cfr13I GGNCC 4 cut(s) 124, 827, 941, 1009
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 3 cut(s) 278, 421, 684
CviAII CATG 5 cut(s) 347, 362, 442, 593, 614
CviQI GTAC 3 cut(s) 278, 421, 684
DdeI CTNAG 5 cut(s) 330, 351, 467, 496, 1013
DpnI GATC 3 cut(s) 93, 369, 933
DpnII GATC 3 cut(s) 91, 367, 931
Eco130I CCWWGG 2 cut(s) 361, 708
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 3 cut(s) 124, 827, 941
Eco47III AGCGCT 1 cut(s) 522
Eco57I CTGAAG 2 cut(s) 911, 978
Eco81I CCTNAGG 1 cut(s) 1013
Eco91I GGTNACC 1 cut(s) 977
EcoO109I RGGNCCY 1 cut(s) 1009
EcoO65I GGTNACC 1 cut(s) 977
EcoRII CCWGG 2 cut(s) 126, 232
EcoT14I CCWWGG 2 cut(s) 361, 708
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 361, 708
FaeI CATG 5 cut(s) 350, 365, 445, 596, 617
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FatI CATG 5 cut(s) 346, 361, 441, 592, 613
FauI CCCGC 1 cut(s) 902
Fnu4HI GCNGC 8 cut(s) 6, 9, 150, 429, 483, 639, 1020, 1023
FokI GGATG 2 cut(s) 311, 613
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 8 cut(s) 6, 9, 150, 429, 483, 639, 1020, 1023
FspBI CTAG 3 cut(s) 486, 605, 993
GlaI GCGC 2 cut(s) 522, 574
GluI GCNGC 8 cut(s) 6, 9, 150, 429, 483, 639, 1020, 1023
GsaI CCCAGC 2 cut(s) 407, 579
HaeII RGCGCY 2 cut(s) 524, 576
HaeIII GGCC 1 cut(s) 1010
HapII CCGG 1 cut(s) 842
HhaI GCGC 2 cut(s) 523, 575
Hin1II CATG 5 cut(s) 350, 365, 445, 596, 617
Hin6I GCGC 2 cut(s) 521, 573
HinP1I GCGC 2 cut(s) 521, 573
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 2 cut(s) 334, 598
HpaII CCGG 1 cut(s) 842
HphI GGTGA 6 cut(s) 221, 271, 400, 802, 853, 928
Hpy166II GTNNAC 2 cut(s) 423, 861
Hpy188I TCNGA 4 cut(s) 58, 84, 468, 958
Hpy188III TCNNGA 2 cut(s) 110, 993
Hpy8I GTNNAC 2 cut(s) 423, 861
HpyAV CCTTC 9 cut(s) 39, 200, 256, 500, 778, 787, 791, 799, 907
HpyCH4V TGCA 6 cut(s) 8, 24, 74, 431, 647, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 644, 785, 1016
HpyF3I CTNAG 5 cut(s) 330, 351, 467, 496, 1013
Hsp92II CATG 5 cut(s) 350, 365, 445, 596, 617
HspAI GCGC 2 cut(s) 521, 573
Kzo9I GATC 3 cut(s) 91, 367, 931
LmnI GCTCC 3 cut(s) 16, 518, 535
Lsp1109I GCAGC 7 cut(s) 20, 136, 415, 469, 650, 1006, 1009
LweI GCATC 2 cut(s) 635, 745
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 3 cut(s) 486, 605, 993
MaeIII GTNAC 3 cut(s) 811, 832, 977
MalI GATC 3 cut(s) 93, 369, 933
MbiI CCGCTC 2 cut(s) 533, 909
MboI GATC 3 cut(s) 91, 367, 931
MboII GAAGA 1 cut(s) 734
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 5 cut(s) 86, 114, 228, 737, 851
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 4 cut(s) 115, 563, 849, 1008
MseI TTAA 4 cut(s) 117, 159, 288, 671
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 842
MspR9I CCNGG 2 cut(s) 128, 234
MvaI CCWGG 2 cut(s) 128, 234
MwoI GCNNNNNNNGC 4 cut(s) 518, 644, 785, 1016
NcoI CCATGG 1 cut(s) 361
NdeII GATC 3 cut(s) 91, 367, 931
NheI GCTAGC 2 cut(s) 485, 604
NlaIII CATG 5 cut(s) 350, 365, 445, 596, 617
NmuCI GTSAC 1 cut(s) 811
NspI RCATGY 1 cut(s) 596
PciI ACATGT 1 cut(s) 592
PfeI GAWTC 2 cut(s) 334, 598
PkrI GCNGC 8 cut(s) 7, 10, 151, 430, 484, 640, 1021, 1024
PscI ACATGT 1 cut(s) 592
PshAI GACNNNNGTC 1 cut(s) 818
Psp6I CCWGG 2 cut(s) 126, 232
PspEI GGTNACC 1 cut(s) 977
PspFI CCCAGC 2 cut(s) 403, 575
PspGI CCWGG 2 cut(s) 126, 232
PspPI GGNCC 4 cut(s) 124, 827, 941, 1009
PstI CTGCAG 3 cut(s) 10, 76, 433
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 3 cut(s) 279, 422, 685
RsaNI GTAC 3 cut(s) 278, 421, 684
SaqAI TTAA 4 cut(s) 117, 159, 288, 671
SatI GCNGC 8 cut(s) 6, 9, 150, 429, 483, 639, 1020, 1023
Sau3AI GATC 3 cut(s) 91, 367, 931
Sau96I GGNCC 4 cut(s) 124, 827, 941, 1009
ScrFI CCNGG 2 cut(s) 128, 234
SduI GDGCHC 1 cut(s) 184
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 2 cut(s) 635, 745
SfcI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
SinI GGWCC 3 cut(s) 124, 827, 941
SmlI CTYRAG 1 cut(s) 983
SmoI CTYRAG 1 cut(s) 983
Sse9I AATT 5 cut(s) 86, 114, 228, 737, 851
SsiI CCGC 3 cut(s) 533, 788, 909
SspMI CTAG 3 cut(s) 486, 605, 993
StyD4I CCNGG 2 cut(s) 126, 232
StyI CCWWGG 2 cut(s) 361, 708
TaqI TCGA 1 cut(s) 720
TasI AATT 5 cut(s) 86, 114, 228, 737, 851
TatI WGTACW 2 cut(s) 277, 420
TfiI GAWTC 2 cut(s) 334, 598
Tru1I TTAA 4 cut(s) 117, 159, 288, 671
Tru9I TTAA 4 cut(s) 117, 159, 288, 671
TscAI CASTG 3 cut(s) 334, 786, 930
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 8 cut(s) 5, 8, 149, 428, 482, 638, 1019, 1022
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 1 cut(s) 315
TspRI CASTG 3 cut(s) 334, 786, 930
VpaK11BI GGWCC 3 cut(s) 124, 827, 941
XapI RAATTY 3 cut(s) 86, 737, 851
XbaI TCTAGA 1 cut(s) 992
XceI RCATGY 1 cut(s) 596
XspI CTAG 3 cut(s) 486, 605, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.