MD17G1061400.v1.1

FCP1 homology domain-containing protein C1271.03c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000879
Physical Location & Seq
Reverse (-)
4979815 .. 4983879
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1061400.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGATCAAGAGTACTAGTTCCTTTACAATGCCCAAGGGTGAAGTCCAAGTTATTCTCTTAGTGAATGCAGGACCTCGGCCTTCCATTATTAGAATTATGGAGACAGGTACTGAAACAACAAAAAATCATGCGCAGTCGAATCAAGTGTTGAAAAAGAAAACAAGAAGGAGACATAGGATGAAGAAGCCAAATTTACTGCAATACACAGGTCTAACTGAGAACCTGCCTCAGGCATATGGTCAGAGTGATGCTAATCATATGCATGGATTGTCAACTTTAAGTCTATCTGATGAAAGAAGTGATGACGTCGTAGTTATTTCATCAGGACGTGTTAAAACCATGAATGAAGAGGACAACAGATCTGGGATTTCAAATATCTCAATTGTAAGAACACCTGTTTGCAATACAGAGAAAAAGCTTCTTATTCTTGATATAAATGGACTACTTGCAGATATAGTATCTCCTCCTCCAAAGGGACTTCCATCTGACATAAGAATTCAAGGGCGAGCAATTTTCAAGAGGCCCTTCTATCTTGATTTTCTTAAGTTCTGCTTTGAGCATTTCGAAGTGGGTGTGTGGTCTTCAAGATCCAAGAGAATTGTGGAAAAAGTGATTGATTATCTGATGGGTGATATGAAGCACAAGTTGCTGTTTTGTTGGGATCTATCCCACTGTACTGCAACAGGGTTCAGGACTCTAGAAAATAAGCATAAGACCTTGGTGTTTAAGGAGCTGAGGAGAATATGGGAAAAACAAGACCCTAGTCTTCCGTGGGAGAAGGGCGCATATAACGAATCAAACACAGTGTTGTTGGATGATTCTCCCTACAAAGCCTTGCTTAATCCTGCACATACTTCAGTGTTTCCTTATCCTTTCACGTTCCAGCGAGGGAGAGACACTTCATTAGGTCCTGGAGGTGATATTCGGGTCTATCTGGAACGGTTGGCTGCAGCTGAAAATATACAGGAATTTGTAGAGCAACAGCCATTTGGCCAAGGACCTATTAATGAAAGTAGTGCATCTTGGCCATTCTACCTCAAGGTTCTTAGAAAGGTGTATGCTATAGAAACCGCCAACATAACCTGTAGTTCTACGGTGAAGCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

369

Amino Acids

41.8

Weight (kDa)

9.4

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 139 - 319 2.4e-16 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
Acc16I TGCGCA 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 231
AciI CCGC 1 cut(s) 1071
AclWI GGATC 2 cut(s) 582, 669
AcoI YGGCCR 2 cut(s) 991, 1025
AcsI RAATTY 3 cut(s) 190, 495, 968
AcuI CTGAAG 1 cut(s) 840
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 3 cut(s) 13, 109, 676
AfiI CCNNNNNNNGG 2 cut(s) 229, 473
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 5 cut(s) 151, 372, 500, 517, 585
AhlI ACTAGT 1 cut(s) 14
AjiI CACGTC 1 cut(s) 329
AjnI CCWGG 1 cut(s) 910
AluBI AGCT 3 cut(s) 418, 733, 953
AluI AGCT 3 cut(s) 418, 733, 953
Alw26I GTCTC 3 cut(s) 95, 163, 888
AlwI GGATC 2 cut(s) 582, 669
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 4 cut(s) 77, 521, 991, 1025
ApeKI GCWGC 2 cut(s) 947, 950
ApoI RAATTY 3 cut(s) 190, 495, 968
AseI ATTAAT 1 cut(s) 1005
AspLEI GCGC 2 cut(s) 133, 785
AspS9I GGNCC 4 cut(s) 71, 522, 908, 998
AsuHPI GGTGA 3 cut(s) 50, 641, 929
AsuII TTCGAA 1 cut(s) 564
AvaII GGWCC 3 cut(s) 71, 908, 998
AxyI CCTNAGG 1 cut(s) 228
BalI TGGCCA 2 cut(s) 993, 1027
BbsI GAAGAC 2 cut(s) 573, 758
BbvCI CCTCAGC 1 cut(s) 734
BbvI GCAGC 2 cut(s) 934, 962
BccI CCATC 2 cut(s) 490, 619
BciT130I CCWGG 1 cut(s) 912
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 95, 163, 888
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 3 cut(s) 15, 698, 762
BfmI CTRYAG 3 cut(s) 948, 1062, 1084
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 231
BglII AGATCT 1 cut(s) 359
BisI GCNGC 2 cut(s) 948, 951
BlsI GCNGC 2 cut(s) 949, 952
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 912
Bme18I GGWCC 3 cut(s) 71, 908, 998
BmgBI CACGTC 1 cut(s) 329
BmgT120I GGNCC 4 cut(s) 71, 522, 908, 998
BmrFI CCNGG 1 cut(s) 912
BmsI GCATC 2 cut(s) 238, 1028
BoxI GACNNNNGTC 1 cut(s) 762
BpiI GAAGAC 2 cut(s) 573, 758
BpmI CTGGAG 1 cut(s) 933
Bpu10I CCTNAGC 1 cut(s) 734
Bpu14I TTCGAA 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 5 cut(s) 33, 74, 717, 770, 994
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 473
Bse21I CCTNAGG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 5 cut(s) 33, 74, 717, 770, 994
BseGI GGATG 2 cut(s) 183, 820
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 229, 473
BseMII CTCAG 3 cut(s) 207, 242, 725
BseRI GAGGAG 3 cut(s) 453, 456, 751
BseXI GCAGC 2 cut(s) 934, 962
BsgI GTGCAG 1 cut(s) 831
BshFI GGCC 4 cut(s) 79, 523, 993, 1027
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 229, 473
BsmAI GTCTC 3 cut(s) 95, 163, 888
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 4 cut(s) 79, 523, 993, 1027
Bsp119I TTCGAA 1 cut(s) 564
Bsp143I GATC 4 cut(s) 3, 359, 587, 661
BspACI CCGC 1 cut(s) 1071
BspANI GGCC 4 cut(s) 79, 523, 993, 1027
BspCNI CTCAG 3 cut(s) 208, 241, 726
BspMAI CTGCAG 1 cut(s) 952
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 2 cut(s) 582, 669
BspT104I TTCGAA 1 cut(s) 564
BspTI CTTAAG 1 cut(s) 542
BssECI CCNNGG 5 cut(s) 33, 74, 717, 770, 994
BssMI GATC 4 cut(s) 3, 359, 587, 661
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 3 cut(s) 33, 717, 994
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 4 cut(s) 674, 805, 942, 1096
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 306
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 1 cut(s) 646
BstBI TTCGAA 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 5 cut(s) 58, 216, 228, 734, 1046
BstDSI CCRYGG 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 183, 820
BstHHI GCGC 2 cut(s) 133, 785
BstKTI GATC 4 cut(s) 6, 362, 590, 664
BstMAI GTCTC 3 cut(s) 95, 163, 888
BstMBI GATC 4 cut(s) 3, 359, 587, 661
BstMWI GCNNNNNNNGC 1 cut(s) 646
BstNI CCWGG 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 762
BstSCI CCNGG 1 cut(s) 910
BstSFI CTRYAG 3 cut(s) 948, 1062, 1084
BstV1I GCAGC 2 cut(s) 934, 962
BstV2I GAAGAC 2 cut(s) 573, 758
BstX2I RGATCY 3 cut(s) 359, 587, 661
BstYI RGATCY 3 cut(s) 359, 587, 661
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 4 cut(s) 79, 523, 993, 1027
BtgI CCRYGG 1 cut(s) 770
BtrI CACGTC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 183, 820
BtsIMutI CAGTG 3 cut(s) 670, 810, 864
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 507
CaiI CAGNNNCTG 1 cut(s) 110
CfoI GCGC 2 cut(s) 133, 785
Cfr13I GGNCC 4 cut(s) 71, 522, 908, 998
Csp6I GTAC 3 cut(s) 12, 108, 675
CviAII CATG 3 cut(s) 128, 263, 340
CviQI GTAC 3 cut(s) 12, 108, 675
DdeI CTNAG 5 cut(s) 58, 216, 228, 734, 1046
DpnI GATC 4 cut(s) 5, 361, 589, 663
DpnII GATC 4 cut(s) 3, 359, 587, 661
EaeI YGGCCR 2 cut(s) 991, 1025
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 33, 717, 994
Eco47I GGWCC 3 cut(s) 71, 908, 998
Eco57I CTGAAG 1 cut(s) 840
Eco81I CCTNAGG 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 4 cut(s) 71, 522, 908, 998
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 3 cut(s) 33, 717, 994
EcoT22I ATGCAT 1 cut(s) 264
ErhI CCWWGG 3 cut(s) 33, 717, 994
FaeI CATG 3 cut(s) 131, 266, 343
FalI AAGNNNNNCTT 6 cut(s) 509, 541, 536, 568, 822, 854
FaqI GGGAC 1 cut(s) 489
FatI CATG 3 cut(s) 127, 262, 339
FauNDI CATATG 2 cut(s) 235, 258
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 948, 951
FokI GGATG 2 cut(s) 190, 827
Fsp4HI GCNGC 2 cut(s) 948, 951
FspBI CTAG 3 cut(s) 15, 698, 762
FspI TGCGCA 1 cut(s) 132
GlaI GCGC 2 cut(s) 132, 784
GluI GCNGC 2 cut(s) 948, 951
GsuI CTGGAG 1 cut(s) 933
HaeIII GGCC 4 cut(s) 79, 523, 993, 1027
HhaI GCGC 2 cut(s) 133, 785
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 3 cut(s) 131, 266, 343
Hin6I GCGC 2 cut(s) 131, 783
HinP1I GCGC 2 cut(s) 131, 783
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 4 cut(s) 139, 694, 794, 818
HphI GGTGA 3 cut(s) 50, 641, 929
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 4 cut(s) 243, 289, 487, 624
Hpy188III TCNNGA 9 cut(s) 7, 324, 428, 517, 533, 585, 691, 698, 935
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 90, 159, 535, 772
HpyCH4III ACNGT 4 cut(s) 674, 805, 942, 1096
HpyCH4IV ACGT 3 cut(s) 306, 328, 878
HpyCH4V TGCA 9 cut(s) 68, 199, 262, 402, 449, 680, 848, 950, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 646
HpyF3I CTNAG 5 cut(s) 58, 216, 228, 734, 1046
HpySE526I ACGT 3 cut(s) 306, 328, 878
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 3 cut(s) 131, 266, 343
HspAI GCGC 2 cut(s) 131, 783
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 359, 587, 661
LmnI GCTCC 1 cut(s) 730
Lsp1109I GCAGC 2 cut(s) 934, 962
LweI GCATC 2 cut(s) 238, 1028
MaeI CTAG 3 cut(s) 15, 698, 762
MaeII ACGT 3 cut(s) 306, 328, 878
MalI GATC 4 cut(s) 5, 361, 589, 663
MboI GATC 4 cut(s) 3, 359, 587, 661
MboII GAAGA 4 cut(s) 193, 359, 573, 758
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 3 cut(s) 359, 587, 661
MlsI TGGCCA 2 cut(s) 993, 1027
MluCI AATT 7 cut(s) 93, 190, 381, 495, 510, 597, 968
MluNI TGGCCA 2 cut(s) 993, 1027
MlyI GAGTC 1 cut(s) 688
MmeI TCCRAC 1 cut(s) 792
Mox20I TGGCCA 2 cut(s) 993, 1027
Mph1103I ATGCAT 1 cut(s) 264
MscI TGGCCA 2 cut(s) 993, 1027
MseI TTAA 6 cut(s) 278, 333, 543, 726, 840, 1005
MslI CAYNNNNRTG 1 cut(s) 261
Msp20I TGGCCA 2 cut(s) 993, 1027
MspA1I CMGCKG 1 cut(s) 953
MspCI CTTAAG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 912
MunI CAATTG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 1 cut(s) 912
MwoI GCNNNNNNNGC 1 cut(s) 646
NdeI CATATG 2 cut(s) 235, 258
NdeII GATC 4 cut(s) 3, 359, 587, 661
NlaIII CATG 3 cut(s) 131, 266, 343
NmeAIII GCCGAG 1 cut(s) 55
NsbI TGCGCA 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 264
NspV TTCGAA 1 cut(s) 564
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 3 cut(s) 139, 794, 818
PfoI TCCNGGA 1 cut(s) 910
PkrI GCNGC 2 cut(s) 949, 952
PleI GAGTC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 688
PpuMI RGGWCCY 3 cut(s) 71, 908, 998
PshAI GACNNNNGTC 1 cut(s) 762
PshBI ATTAAT 1 cut(s) 1005
Psp5II RGGWCCY 3 cut(s) 71, 908, 998
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspPI GGNCC 4 cut(s) 71, 522, 908, 998
PspPPI RGGWCCY 3 cut(s) 71, 908, 998
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 3 cut(s) 359, 587, 661
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 3 cut(s) 13, 109, 676
RsaNI GTAC 3 cut(s) 12, 108, 675
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 6 cut(s) 278, 333, 543, 726, 840, 1005
SatI GCNGC 2 cut(s) 948, 951
Sau3AI GATC 4 cut(s) 3, 359, 587, 661
Sau96I GGNCC 4 cut(s) 71, 522, 908, 998
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 688
ScrFI CCNGG 1 cut(s) 912
SfaNI GCATC 2 cut(s) 238, 1028
SfcI CTRYAG 3 cut(s) 948, 1062, 1084
SfuI TTCGAA 1 cut(s) 564
SinI GGWCC 3 cut(s) 71, 908, 998
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 542, 1037
SmoI CTYRAG 2 cut(s) 542, 1037
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 7 cut(s) 93, 190, 381, 495, 510, 597, 968
SsiI CCGC 1 cut(s) 1071
SspMI CTAG 3 cut(s) 15, 698, 762
StyD4I CCNGG 1 cut(s) 910
StyI CCWWGG 3 cut(s) 33, 717, 994
TaaI ACNGT 4 cut(s) 674, 805, 942, 1096
TaiI ACGT 3 cut(s) 309, 331, 881
TaqI TCGA 2 cut(s) 137, 564
TasI AATT 7 cut(s) 93, 190, 381, 495, 510, 597, 968
TatI WGTACW 2 cut(s) 11, 674
TfiI GAWTC 3 cut(s) 139, 794, 818
Tru1I TTAA 6 cut(s) 278, 333, 543, 726, 840, 1005
Tru9I TTAA 6 cut(s) 278, 333, 543, 726, 840, 1005
TscAI CASTG 3 cut(s) 677, 810, 864
TseI GCWGC 2 cut(s) 947, 950
TspDTI ATGAA 8 cut(s) 194, 306, 309, 356, 360, 650, 891, 1023
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 3 cut(s) 677, 810, 864
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 3 cut(s) 71, 908, 998
VspI ATTAAT 1 cut(s) 1005
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 3 cut(s) 190, 495, 968
XbaI TCTAGA 1 cut(s) 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XspI CTAG 3 cut(s) 15, 698, 762
ZraI GACGTC 1 cut(s) 307
ZrmI AGTACT 1 cut(s) 13
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.