pycom01g20700
MYB Family

response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001432
Physical Location & Seq
Forward (+)
19020823 .. 19025556
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20700.5

Sequence Viewer

Length: 2340 bp
ATGGATGCTACAGATAGTGCTGAGTTTAGGCAATCTTCTTCCATTTCCAAACCCTCTTTCGTTGGTATGATCAATATCTTGGTTGTGGACGACGATGCCACCACTCTAGCTATTGTTTCAGCAATGCTCAAAACATGGAGCTATCAAGTTGTCGCTGTTAAGAATCCTATGGATGCTTTGGCTATTCTTCGAGCACAAAAAGGCGCATTTGATCTTGTTGTTACGGACCTCCATATGCCTCAGATGGATGGGTTTGAATTACACAAGCATGTTCGTGATGAGTTTAAGCTACCTGTTATTATGATGTCCGCAGATGACAACGAGAATGTGATACTAAAGAGTTTAGAGGGCGGATTTGCTGATTATATGGTAAAGCCTGTGAGCAAAGATGACATTAAAAATGTATGGCAGTATGCTGAGGCAGCTAAGAAAGGTAAATCTGTTGTCATAGAGGAAATAGAAGGTGCTCCAAGAGAAGCACCTGGTGAGGTGGACAATGGAAGTAACTCTGGGATGTCTTCGGATACTGATGAGGAACAACATGAGAAAAAGGCCGAGGCAAAGGCGAAGAAGTCAAAAAAGAGAAATAGGGAAGGCGATAATGAGGAACGCATGGTTGCACCAAAGAAGCCAAAAGTGGTGTGGACAAATTCACTTCACAATCAGTTTCTATTGGCCATTAGACACATCGGTTTGGACAAGGCAGTGCCAAAGAGGATTCTCGAGTTCATGAATGTGCCAGGCTTGACCAGGGAAAATGTAGCTAGTCATTTGCAGAAGTATCGCATGTTCTTGAAACGAGTTGCTGAGAAAGCTAGACTTTCCAAGTGCTTGTCTGAGAGGGTGTTCAGGTCAAGCTTTGCATTTAGCCATCCCGTACCGGTCTTCAATAGTATGGAAGCAGGAGACCAATATTCACAGTACCTGAAATTACAACAGCAAATGGGAGCATCTCCAATCAATATTGCCTCTGCATTTCAACCATCCTCAGCTGGGATTGATGGAAATACCATTAATCTCCTTGGCTCAATCCAATACCCTAATTATCAGCAATCTTCATGCAGCAGCAACACCATGGGATCCATGCCTCAACTTATCGGTTCGGGGCAGTCCCGTCTGCTGAACCATATCACTCAAGCTAATCTTCTCCGCCATCAACCATGGCTTGGAAATGTGAATCATCAGCTGATTTCTCAAGCAAGTCGCCGCCCAGGGGTTATTGGAATGCAACAACTGATGAACAATTTTGGATCAAGTGGAGGCATGTCTTCTAATGCTGCCAATACTACTGGACTTACCACCAATAATATTATTACAAATAATTTGATCCAAATTTTCCAACAAGCAAGGCAGCATGGCATTTTTACCAATAACAACCCATTCAGCTACAACTTTGGCACACCTGGAATTCAGAACAATGTTACTAATCAGCAGCTGATTGGTTTTTCAGGAACCCAAATGATGAACCCTAATGGCAGATCTGAGCTACTGGGAATACTCAATAATTTCAATGGGACTGGTGGATTTTCATCAAATGGGTTTAACTTCAACAATGAGACTATCAATGGCTTGACCAATAGGGTTCGAAACGTGAGTCTGAGTGGTACTACTCCACCAATGGCTACCTTAGGAGGTTCTTCATCGTTGTCGCCTTCTGCTGGGATTGGGATTAGCACTACTACTTCTAGTGCATATCAGTTTCAAAAAGATTTCAGTAACAACAGAGAAGATCAGCAACAAAATGTTTCAGTGTTGCCACCGCCAGCAGAGAATACCGATGATAATAGCACTACTACTCCTACATTGGTCAACAAGAATAATTTGTCTCCAGGACAAGAAATGGACAAAGCTCTTGATGATTTGATGATTAGTAATCTTTTCGTGGACCAGCAGAACAACTATCAGCCTCCTCCTTCTTCTCAGCAACAAGGTGGAGATCAAGATCAACTTCCTCCTGACTTCTCTAGTTTCTTATATCCAGTTGAAGTGCCGGACGCTACTCCGAATCAAATGAACCCTGCTGTTGCGGACCAAGGTCTGGACACTGCTGTGAACAAAGTTTCCGACCCTTCTCCTGACCAGGTCCCAAAACCGAACTCTTCTGGCACATACTGTGTCGAAGAAAATGCACCTAATGAACAATCTTGGAGCCAGGTCACAAACCCTAAATCTTCTGGCCCATACCATGTTGAAGAAAACGGACCTAATGAACAATCTTGGAGCCAGGCATGCATGTTCATTTCATATTTTAAGCACGTACACACGCGCGCGCACAAACACACAAAGGATTCCATCACGATACTAATTATATTGTGGTTGTGTGCAGCAGCAGGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Myb_DNA-binding Response_reg

Protein Analysis

780

Amino Acids

85.39

Weight (kDa)

6.05

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1166, 2038
AccII CGCG 3 cut(s) 2266, 2268, 2270
AciI CCGC 6 cut(s) 309, 351, 1150, 1207, 1760, 2027
AclWI GGATC 4 cut(s) 1074, 1087, 1258, 1321
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 3 cut(s) 649, 1332, 1407
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 4 cut(s) 879, 923, 1606, 2259
AfiI CCNNNNNNNGG 5 cut(s) 993, 1166, 1213, 1658, 2038
AgeI ACCGGT 1 cut(s) 880
AgsI TTSAA 9 cut(s) 257, 796, 889, 980, 1510, 1549, 1703, 1985, 2192
AjnI CCWGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AleI CACNNNNGTG 1 cut(s) 2048
AloI GAACNNNNNNTCC 2 cut(s) 2045, 2077
Alw21I GWGCWC 2 cut(s) 196, 469
Alw26I GTCTC 3 cut(s) 900, 1550, 1830
AlwI GGATC 4 cut(s) 1074, 1087, 1258, 1321
AlwNI CAGNNNCTG 2 cut(s) 925, 1435
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 3 cut(s) 552, 675, 2176
ApeKI GCWGC 8 cut(s) 422, 1062, 1065, 1277, 1351, 1432, 2324, 2327
ApoI RAATTY 3 cut(s) 649, 1332, 1407
ArsI GACNNNNNNTTYG 2 cut(s) 1579, 1611
AseI ATTAAT 1 cut(s) 1014
AsiGI ACCGGT 1 cut(s) 880
AspLEI GCGC 4 cut(s) 206, 2268, 2270, 2272
AspS9I GGNCC 6 cut(s) 226, 1885, 2029, 2083, 2177, 2201
AsuHPI GGTGA 1 cut(s) 497
AsuII TTCGAA 1 cut(s) 1585
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 5 cut(s) 226, 1885, 2029, 2083, 2201
AxyI CCTNAGG 1 cut(s) 1627
BalI TGGCCA 1 cut(s) 677
BamHI GGATCC 1 cut(s) 1079
BbsI GAAGAC 3 cut(s) 510, 877, 1260
Bbv12I GWGCWC 2 cut(s) 196, 469
BbvCI CCTCAGC 2 cut(s) 417, 988
BbvI GCAGC 7 cut(s) 434, 1074, 1077, 1264, 1363, 1444, 2336
BccI CCATC 7 cut(s) 238, 242, 879, 991, 995, 1161, 2300
BcgI CGANNNNNNTGC 4 cut(s) 764, 798, 2108, 2142
BciT130I CCWGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 3 cut(s) 900, 1550, 1830
BfaI CTAG 5 cut(s) 107, 765, 816, 1686, 1965
BfmI CTRYAG 1 cut(s) 9
BfuI GTATCC 1 cut(s) 517
BglII AGATCT 1 cut(s) 1478
BisI GCNGC 9 cut(s) 423, 1063, 1066, 1207, 1278, 1352, 1433, 2325, 2328
BlsI GCNGC 9 cut(s) 424, 1064, 1067, 1208, 1279, 1353, 1434, 2326, 2329
Bme1390I CCNGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
Bme18I GGWCC 5 cut(s) 226, 1885, 2029, 2083, 2201
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 6 cut(s) 226, 1885, 2029, 2083, 2177, 2201
BmiI GGNNCC 5 cut(s) 1081, 1453, 2085, 2150, 2222
BmrFI CCNGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 3 cut(s) 85, 163, 959
BmuI ACTGGG 1 cut(s) 1499
BoxI GACNNNNGTC 1 cut(s) 2034
BpiI GAAGAC 3 cut(s) 510, 877, 1260
BpmI CTGGAG 1 cut(s) 1812
Bpu10I CCTNAGC 2 cut(s) 417, 988
Bpu14I TTCGAA 1 cut(s) 1585
BpuEI CTTGAG 2 cut(s) 1119, 1179
BsaAI YACGTR 1 cut(s) 2257
BsaBI GATNNNNATC 3 cut(s) 74, 1528, 1941
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 8 cut(s) 555, 750, 1021, 1074, 1160, 1210, 1211, 2032
BsaWI WCCGGW 1 cut(s) 880
BsaXI ACNNNNNCTCC 2 cut(s) 1780, 1810
Bsc4I CCNNNNNNNGG 5 cut(s) 993, 1166, 1213, 1658, 2038
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 4 cut(s) 1294, 1494, 1522, 1979
Bse21I CCTNAGG 1 cut(s) 1627
Bse3DI GCAATG 1 cut(s) 129
Bse8I GATNNNNATC 3 cut(s) 74, 1528, 1941
BseBI CCWGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
BseDI CCNNGG 8 cut(s) 555, 750, 1021, 1074, 1160, 1210, 1211, 2032
BseGI GGATG 6 cut(s) 10, 178, 253, 519, 871, 983
BseJI GATNNNNATC 3 cut(s) 74, 1528, 1941
BseLI CCNNNNNNNGG 5 cut(s) 993, 1166, 1213, 1658, 2038
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 9 cut(s) 12, 254, 408, 798, 828, 1002, 1473, 1589, 1934
BseNI ACTGG 4 cut(s) 1294, 1494, 1522, 1979
BsePI GCGCGC 2 cut(s) 2266, 2268
BseRI GAGGAG 1 cut(s) 1899
BseXI GCAGC 7 cut(s) 434, 1074, 1077, 1264, 1363, 1444, 2336
BseYI CCCAGC 2 cut(s) 992, 1658
Bsh1236I CGCG 3 cut(s) 2266, 2268, 2270
BshFI GGCC 3 cut(s) 554, 677, 2178
BshTI ACCGGT 1 cut(s) 880
BsiHKAI GWGCWC 2 cut(s) 196, 469
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 2 cut(s) 881, 1991
BslFI GGGAC 3 cut(s) 1096, 1528, 2069
BslI CCNNNNNNNGG 5 cut(s) 993, 1166, 1213, 1658, 2038
BsmAI GTCTC 3 cut(s) 900, 1550, 1830
BsmFI GGGAC 3 cut(s) 1096, 1528, 2069
BsmI GAATGC 1 cut(s) 1230
BsnI GGCC 3 cut(s) 554, 677, 2178
Bso31I GGTCTC 1 cut(s) 900
BsoBI CYCGRG 1 cut(s) 722
Bsp119I TTCGAA 1 cut(s) 1585
Bsp1286I GDGCHC 2 cut(s) 196, 469
Bsp143I GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
Bsp19I CCATGG 2 cut(s) 1074, 1160
BspACI CCGC 6 cut(s) 309, 351, 1150, 1207, 1760, 2027
BspANI GGCC 3 cut(s) 554, 677, 2178
BspCNI CTCAG 9 cut(s) 13, 253, 409, 799, 829, 1001, 1474, 1590, 1933
BspFNI CGCG 3 cut(s) 2266, 2268, 2270
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 5 cut(s) 1081, 1453, 2085, 2150, 2222
BspPI GGATC 4 cut(s) 1074, 1087, 1258, 1321
BspT104I TTCGAA 1 cut(s) 1585
BspTNI GGTCTC 1 cut(s) 900
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 880
BsrI ACTGG 4 cut(s) 1294, 1494, 1522, 1979
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 8 cut(s) 555, 750, 1021, 1074, 1160, 1210, 1211, 2032
BssHII GCGCGC 2 cut(s) 2266, 2268
BssMI GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
BssT1I CCWWGG 4 cut(s) 1021, 1074, 1160, 2032
Bst2UI CCWGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
Bst4CI ACNGT 2 cut(s) 921, 2114
Bst6I CTCTTC 1 cut(s) 2104
BstBAI YACGTR 1 cut(s) 2257
BstBI TTCGAA 1 cut(s) 1585
BstC8I GCNNGC 4 cut(s) 1764, 2230, 2268, 2270
BstDSI CCRYGG 2 cut(s) 1074, 1160
BstF5I GGATG 6 cut(s) 10, 178, 253, 519, 871, 983
BstFNI CGCG 3 cut(s) 2266, 2268, 2270
BstHHI GCGC 4 cut(s) 206, 2268, 2270, 2272
BstKTI GATC 9 cut(s) 72, 214, 1082, 1253, 1329, 1481, 1732, 1939, 1945
BstMAI GTCTC 3 cut(s) 900, 1550, 1830
BstMBI GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
BstMWI GCNNNNNNNGC 3 cut(s) 422, 812, 2229
BstNI CCWGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
BstNSI RCATGY 5 cut(s) 272, 790, 1267, 2232, 2236
BstPAI GACNNNNGTC 1 cut(s) 2034
BstSCI CCNGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 3 cut(s) 2266, 2268, 2270
BstV1I GCAGC 7 cut(s) 434, 1074, 1077, 1264, 1363, 1444, 2336
BstV2I GAAGAC 3 cut(s) 510, 877, 1260
BstX2I RGATCY 2 cut(s) 1079, 1478
BstYI RGATCY 2 cut(s) 1079, 1478
Bsu36I CCTNAGG 1 cut(s) 1627
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 3 cut(s) 554, 677, 2178
BtgI CCRYGG 2 cut(s) 1074, 1160
BtsCI GGATG 6 cut(s) 10, 178, 253, 519, 871, 983
BtsI GCAGTG 2 cut(s) 711, 2043
BtsIMutI CAGTG 3 cut(s) 711, 1755, 2043
Cac8I GCNNGC 4 cut(s) 1764, 2230, 2268, 2270
CaiI CAGNNNCTG 2 cut(s) 925, 1435
CciI TCATGA 1 cut(s) 729
CfoI GCGC 4 cut(s) 206, 2268, 2270, 2272
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 6 cut(s) 226, 1885, 2029, 2083, 2177, 2201
CseI GACGC 1 cut(s) 2003
CsiI ACCWGGT 2 cut(s) 481, 2079
Csp6I GTAC 4 cut(s) 878, 922, 1605, 2258
CspAI ACCGGT 1 cut(s) 880
CviQI GTAC 4 cut(s) 878, 922, 1605, 2258
DpnI GATC 9 cut(s) 71, 213, 1081, 1252, 1328, 1480, 1731, 1938, 1944
DpnII GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
DraIII CACNNNGTG 1 cut(s) 485
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 1 cut(s) 2104
EarI CTCTTC 1 cut(s) 2104
EciI GGCGGA 2 cut(s) 366, 1139
Eco130I CCWWGG 4 cut(s) 1021, 1074, 1160, 2032
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 5 cut(s) 226, 1885, 2029, 2083, 2201
Eco81I CCTNAGG 1 cut(s) 1627
Eco88I CYCGRG 1 cut(s) 722
EcoO109I RGGNCCY 1 cut(s) 2083
EcoRI GAATTC 1 cut(s) 1407
EcoRII CCWGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
EcoT14I CCWWGG 4 cut(s) 1021, 1074, 1160, 2032
EcoT22I ATGCAT 1 cut(s) 2234
ErhI CCWWGG 4 cut(s) 1021, 1074, 1160, 2032
FalI AAGNNNNNCTT 6 cut(s) 804, 836, 1128, 1160, 1932, 1964
FaqI GGGAC 3 cut(s) 1096, 1528, 2069
FauNDI CATATG 1 cut(s) 234
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 9 cut(s) 423, 1063, 1066, 1207, 1278, 1352, 1433, 2325, 2328
FokI GGATG 6 cut(s) 17, 185, 260, 526, 858, 970
Fsp4HI GCNGC 9 cut(s) 423, 1063, 1066, 1207, 1278, 1352, 1433, 2325, 2328
FspBI CTAG 5 cut(s) 107, 765, 816, 1686, 1965
GlaI GCGC 4 cut(s) 205, 2267, 2269, 2271
GluI GCNGC 9 cut(s) 423, 1063, 1066, 1207, 1278, 1352, 1433, 2325, 2328
GsaI CCCAGC 2 cut(s) 996, 1662
GsuI CTGGAG 1 cut(s) 1812
HaeIII GGCC 3 cut(s) 554, 677, 2178
HapII CCGG 2 cut(s) 881, 1991
HgaI GACGC 1 cut(s) 2003
HhaI GCGC 4 cut(s) 206, 2268, 2270, 2272
Hin6I GCGC 4 cut(s) 204, 2266, 2268, 2270
HinP1I GCGC 4 cut(s) 204, 2266, 2268, 2270
HincII GTYRAC 1 cut(s) 1810
HindII GTYRAC 1 cut(s) 1810
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 6 cut(s) 163, 718, 1177, 1594, 2005, 2288
HpaII CCGG 2 cut(s) 881, 1991
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 7 cut(s) 88, 493, 645, 1810, 1885, 2053, 2260
Hpy188I TCNGA 8 cut(s) 243, 523, 838, 1413, 1483, 1599, 2004, 2065
Hpy8I GTNNAC 7 cut(s) 88, 493, 645, 1810, 1885, 2053, 2260
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 5 cut(s) 455, 587, 1662, 1923, 2079
HpyCH4III ACNGT 2 cut(s) 921, 2114
HpyCH4IV ACGT 2 cut(s) 1590, 2256
HpyF10VI GCNNNNNNNGC 3 cut(s) 422, 812, 2229
HpySE526I ACGT 2 cut(s) 1590, 2256
HspAI GCGC 4 cut(s) 204, 2266, 2268, 2270
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
LmnI GCTCC 5 cut(s) 138, 472, 947, 2148, 2220
Lsp1109I GCAGC 7 cut(s) 434, 1074, 1077, 1264, 1363, 1444, 2336
LweI GCATC 3 cut(s) 85, 163, 959
MabI ACCWGGT 2 cut(s) 481, 2079
MaeI CTAG 5 cut(s) 107, 765, 816, 1686, 1965
MaeII ACGT 2 cut(s) 1590, 2256
MaeIII GTNAC 5 cut(s) 220, 503, 1420, 1715, 2155
MalI GATC 9 cut(s) 71, 213, 1081, 1252, 1328, 1480, 1731, 1938, 1944
MauBI CGCGCGCG 1 cut(s) 2266
MboI GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
MflI RGATCY 2 cut(s) 1079, 1478
MhlI GDGCHC 2 cut(s) 196, 469
MlsI TGGCCA 1 cut(s) 677
MluNI TGGCCA 1 cut(s) 677
MlyI GAGTC 1 cut(s) 1603
MmeI TCCRAC 2 cut(s) 1363, 2088
Mox20I TGGCCA 1 cut(s) 677
Mph1103I ATGCAT 1 cut(s) 2234
MscI TGGCCA 1 cut(s) 677
MseI TTAA 6 cut(s) 159, 285, 396, 1014, 1542, 2250
MslI CAYNNNNRTG 4 cut(s) 267, 273, 734, 2048
Msp20I TGGCCA 1 cut(s) 677
MspA1I CMGCKG 3 cut(s) 992, 1186, 1435
MspI CCGG 2 cut(s) 881, 1991
MspR9I CCNGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
Mva1269I GAATGC 1 cut(s) 1230
MvaI CCWGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
MvnI CGCG 3 cut(s) 2266, 2268, 2270
MwoI GCNNNNNNNGC 3 cut(s) 422, 812, 2229
NcoI CCATGG 2 cut(s) 1074, 1160
NdeI CATATG 1 cut(s) 234
NdeII GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
NlaIV GGNNCC 5 cut(s) 1081, 1453, 2085, 2150, 2222
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 1 cut(s) 2155
NsiI ATGCAT 1 cut(s) 2234
NspI RCATGY 5 cut(s) 272, 790, 1267, 2232, 2236
NspV TTCGAA 1 cut(s) 1585
OliI CACNNNNGTG 1 cut(s) 2048
PaeI GCATGC 1 cut(s) 2232
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 1 cut(s) 729
PasI CCCWGGG 1 cut(s) 1211
PauI GCGCGC 2 cut(s) 2266, 2268
PctI GAATGC 1 cut(s) 1230
PfeI GAWTC 5 cut(s) 163, 718, 1177, 2005, 2288
PflFI GACNNNGTC 1 cut(s) 2081
PflMI CCANNNNNTGG 2 cut(s) 1166, 2038
PfoI TCCNGGA 1 cut(s) 1828
PinAI ACCGGT 1 cut(s) 880
PkrI GCNGC 9 cut(s) 424, 1064, 1067, 1208, 1279, 1353, 1434, 2326, 2329
PleI GAGTC 1 cut(s) 1602
PpsI GAGTC 1 cut(s) 1602
Ppu21I YACGTR 1 cut(s) 2257
PpuMI RGGWCCY 1 cut(s) 2083
PshAI GACNNNNGTC 1 cut(s) 2034
PshBI ATTAAT 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 2083
Psp6I CCWGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
PspFI CCCAGC 2 cut(s) 992, 1658
PspGI CCWGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
PspN4I GGNNCC 5 cut(s) 1081, 1453, 2085, 2150, 2222
PspPI GGNCC 6 cut(s) 226, 1885, 2029, 2083, 2177, 2201
PspPPI RGGWCCY 1 cut(s) 2083
PsrI GAACNNNNNNTAC 2 cut(s) 773, 805
PstNI CAGNNNCTG 2 cut(s) 925, 1435
PsuI RGATCY 2 cut(s) 1079, 1478
PsyI GACNNNGTC 1 cut(s) 2081
PteI GCGCGC 2 cut(s) 2266, 2268
PvuII CAGCTG 3 cut(s) 992, 1186, 1435
RsaI GTAC 4 cut(s) 879, 923, 1606, 2259
RsaNI GTAC 4 cut(s) 878, 922, 1605, 2258
RseI CAYNNNNRTG 4 cut(s) 267, 273, 734, 2048
SaqAI TTAA 6 cut(s) 159, 285, 396, 1014, 1542, 2250
SatI GCNGC 9 cut(s) 423, 1063, 1066, 1207, 1278, 1352, 1433, 2325, 2328
Sau3AI GATC 9 cut(s) 69, 211, 1079, 1250, 1326, 1478, 1729, 1936, 1942
Sau96I GGNCC 6 cut(s) 226, 1885, 2029, 2083, 2177, 2201
SchI GAGTC 1 cut(s) 1603
ScrFI CCNGG 9 cut(s) 483, 741, 751, 1212, 1404, 1830, 2081, 2153, 2225
SduI GDGCHC 2 cut(s) 196, 469
SexAI ACCWGGT 2 cut(s) 481, 2079
SfaNI GCATC 3 cut(s) 85, 163, 959
SfcI CTRYAG 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 722
SfuI TTCGAA 1 cut(s) 1585
SinI GGWCC 5 cut(s) 226, 1885, 2029, 2083, 2201
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 4 cut(s) 267, 273, 734, 2048
SmlI CTYRAG 3 cut(s) 722, 1134, 1194
SmoI CTYRAG 3 cut(s) 722, 1134, 1194
SphI GCATGC 1 cut(s) 2232
SsiI CCGC 6 cut(s) 309, 351, 1150, 1207, 1760, 2027
SspI AATATT 3 cut(s) 914, 964, 1309
SspMI CTAG 5 cut(s) 107, 765, 816, 1686, 1965
StyD4I CCNGG 9 cut(s) 481, 739, 749, 1210, 1402, 1828, 2079, 2151, 2223
StyI CCWWGG 4 cut(s) 1021, 1074, 1160, 2032
TaaI ACNGT 2 cut(s) 921, 2114
TaiI ACGT 2 cut(s) 1593, 2259
TaqI TCGA 4 cut(s) 190, 723, 1585, 2118
TauI GCSGC 1 cut(s) 1209
TfiI GAWTC 5 cut(s) 163, 718, 1177, 2005, 2288
Tru1I TTAA 6 cut(s) 159, 285, 396, 1014, 1542, 2250
Tru9I TTAA 6 cut(s) 159, 285, 396, 1014, 1542, 2250
TscAI CASTG 3 cut(s) 711, 1755, 2050
TseFI GTSAC 1 cut(s) 2155
TseI GCWGC 8 cut(s) 422, 1062, 1065, 1277, 1351, 1432, 2324, 2327
Tsp45I GTSAC 1 cut(s) 2155
TspGWI ACGGA 2 cut(s) 239, 2214
TspRI CASTG 3 cut(s) 711, 1755, 2050
Tth111I GACNNNGTC 1 cut(s) 2081
Van91I CCANNNNNTGG 2 cut(s) 1166, 2038
VpaK11BI GGWCC 5 cut(s) 226, 1885, 2029, 2083, 2201
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 3 cut(s) 649, 1332, 1407
XceI RCATGY 5 cut(s) 272, 790, 1267, 2232, 2236
XcmI CCANNNNNNNNNTGG 1 cut(s) 639
XhoI CTCGAG 1 cut(s) 722
XspI CTAG 5 cut(s) 107, 765, 816, 1686, 1965
Zsp2I ATGCAT 1 cut(s) 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.