pycom02g01370

EG45-like domain containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001334
Physical Location & Seq
Forward (+)
811194 .. 813233
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01370.1

Sequence Viewer

Length: 432 bp
ATGGGAATGGAGATGCGAGTTTTCATTGTGGTGGCCGTGGTGGCTATGTTTATACGCCTTCCCTCCGCAGCATACGCAGACGAGGGAACAGCCACTTATTATACACCTCCTTATGTTCCATCCTCATGCAACGGATACCAAAATGATGGAGTGATGATAGCGGCAGCAAGCGATGCCATTTGGGGCAACAGAGCTGCGTGTGGAAGACGCTACAGTGTGACATGCATCGGAGCCACCAACCAAGGTGTGCCGAAGCCTTGCAAAGGGAATAGCGTTGTCGTTAAAATTGTGGATTACTGTCCTCCTGGATGCCAAGGAACCCGATCGATCTCTCTCAAGAAGCCTTTGCTGCAATTGCTGACCCCAACGCCGGAAAAATCAAGATTCGTTATACCCAGGTTTGATGCAAGCACGCTCTCCAAGAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

144

Amino Acids

15.39

Weight (kDa)

9.11

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 54 - 111 1.7e-09 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 66, 161
AcoI YGGCCR 1 cut(s) 33
AfiI CCNNNNNNNGG 2 cut(s) 263, 370
AjnI CCWGG 2 cut(s) 304, 395
AluBI AGCT 1 cut(s) 194
AluI AGCT 1 cut(s) 194
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 4 cut(s) 68, 164, 194, 349
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 4 cut(s) 80, 176, 181, 336
BccI CCATC 2 cut(s) 127, 140
BceAI ACGGC 1 cut(s) 20
BciT130I CCWGG 2 cut(s) 306, 397
BciVI GTATCC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 211
BfuI GTATCC 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 5 cut(s) 69, 162, 165, 195, 350
BlsI GCNGC 5 cut(s) 70, 163, 166, 196, 351
Bme1390I CCNGG 2 cut(s) 306, 397
BmiI GGNNCC 2 cut(s) 232, 319
BmrFI CCNGG 2 cut(s) 306, 397
BmsI GCATC 5 cut(s) 3, 163, 234, 299, 394
BpiI GAAGAC 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 320
Bsa29I ATCGAT 1 cut(s) 326
BsaJI CCNNGG 4 cut(s) 36, 241, 313, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 370
BseBI CCWGG 2 cut(s) 306, 397
BseCI ATCGAT 1 cut(s) 326
BseDI CCNNGG 4 cut(s) 36, 241, 313, 395
BseGI GGATG 2 cut(s) 119, 314
BseLI CCNNNNNNNGG 2 cut(s) 263, 370
BseXI GCAGC 4 cut(s) 80, 176, 181, 336
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 326
BsiEI CGRYCG 1 cut(s) 326
BsiSI CCGG 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 263, 370
BsnI GGCC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 323, 327
BspACI CCGC 2 cut(s) 66, 161
BspANI GGCC 1 cut(s) 35
BspDI ATCGAT 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 232, 319
BssECI CCNNGG 4 cut(s) 36, 241, 313, 395
BssMI GATC 2 cut(s) 323, 327
BssT1I CCWWGG 2 cut(s) 241, 313
Bst2UI CCWGG 2 cut(s) 306, 397
Bst4CI ACNGT 2 cut(s) 215, 299
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 169, 409, 413
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 119, 314
BstKTI GATC 2 cut(s) 326, 330
BstMBI GATC 2 cut(s) 323, 327
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 5 cut(s) 41, 74, 173, 349, 355
BstNI CCWGG 2 cut(s) 306, 397
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 304, 395
BstSFI CTRYAG 1 cut(s) 211
BstV1I GCAGC 4 cut(s) 80, 176, 181, 336
BstV2I GAAGAC 1 cut(s) 211
BstXI CCANNNNNNTGG 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 326
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 1 cut(s) 35
BsuTUI ATCGAT 1 cut(s) 326
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 119, 314
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 3 cut(s) 169, 409, 413
ClaI ATCGAT 1 cut(s) 326
CseI GACGC 1 cut(s) 216
CviAII CATG 2 cut(s) 126, 222
CviJI RGCY 7 cut(s) 35, 44, 92, 194, 233, 256, 343
CviKI_1 RGCY 7 cut(s) 35, 44, 92, 194, 233, 256, 343
DpnI GATC 2 cut(s) 325, 329
DpnII GATC 2 cut(s) 323, 327
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 241, 313
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 304, 395
EcoT14I CCWWGG 2 cut(s) 241, 313
EcoT22I ATGCAT 1 cut(s) 227
ErhI CCWWGG 2 cut(s) 241, 313
FaeI CATG 2 cut(s) 129, 225
FaiI YATR 8 cut(s) 47, 53, 73, 102, 114, 127, 223, 392
FatI CATG 2 cut(s) 125, 221
Fnu4HI GCNGC 5 cut(s) 69, 162, 165, 195, 350
FokI GGATG 2 cut(s) 106, 321
Fsp4HI GCNGC 5 cut(s) 69, 162, 165, 195, 350
GluI GCNGC 5 cut(s) 69, 162, 165, 195, 350
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 371
HgaI GACGC 1 cut(s) 216
Hin1II CATG 2 cut(s) 129, 225
HinfI GANTC 1 cut(s) 384
HpaII CCGG 1 cut(s) 371
Hpy188I TCNGA 1 cut(s) 230
Hpy188III TCNNGA 2 cut(s) 337, 381
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 2 cut(s) 215, 299
HpyCH4V TGCA 5 cut(s) 129, 225, 261, 352, 407
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 74, 173, 349, 355
Hsp92II CATG 2 cut(s) 129, 225
Kzo9I GATC 2 cut(s) 323, 327
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 5 cut(s) 291, 318, 382, 384, 409
Lsp1109I GCAGC 4 cut(s) 80, 176, 181, 336
LweI GCATC 5 cut(s) 3, 163, 234, 299, 394
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 325, 329
MboI GATC 2 cut(s) 323, 327
MboII GAAGA 1 cut(s) 216
MfeI CAATTG 1 cut(s) 353
MluCI AATT 2 cut(s) 285, 353
MnlI CCTC 5 cut(s) 73, 76, 117, 133, 312
Mph1103I ATGCAT 1 cut(s) 227
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 2 cut(s) 29, 124
MspI CCGG 1 cut(s) 371
MspR9I CCNGG 2 cut(s) 306, 397
MunI CAATTG 1 cut(s) 353
MvaI CCWGG 2 cut(s) 306, 397
MwoI GCNNNNNNNGC 5 cut(s) 41, 74, 173, 349, 355
NdeII GATC 2 cut(s) 323, 327
NlaIII CATG 2 cut(s) 129, 225
NlaIV GGNNCC 2 cut(s) 232, 319
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 227
NspI RCATGY 1 cut(s) 225
PfeI GAWTC 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 5 cut(s) 70, 163, 166, 196, 351
Ple19I CGATCG 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 304, 395
PspGI CCWGG 2 cut(s) 304, 395
PspN4I GGNNCC 2 cut(s) 232, 319
PvuI CGATCG 1 cut(s) 326
RseI CAYNNNNRTG 2 cut(s) 29, 124
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 5 cut(s) 69, 162, 165, 195, 350
Sau3AI GATC 2 cut(s) 323, 327
ScrFI CCNGG 2 cut(s) 306, 397
SetI ASST 4 cut(s) 109, 196, 247, 401
SfaNI GCATC 5 cut(s) 3, 163, 234, 299, 394
SfcI CTRYAG 1 cut(s) 211
SmiMI CAYNNNNRTG 2 cut(s) 29, 124
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 285, 353
SsiI CCGC 2 cut(s) 66, 161
StyD4I CCNGG 2 cut(s) 304, 395
StyI CCWWGG 2 cut(s) 241, 313
TaaI ACNGT 2 cut(s) 215, 299
TaqI TCGA 1 cut(s) 326
TasI AATT 2 cut(s) 285, 353
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 4 cut(s) 68, 164, 194, 349
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 13
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 220
XagI CCTNNNNNAGG 1 cut(s) 261
XceI RCATGY 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.