Rorug04G0049600

EG45-like domain containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001334
Physical Location & Seq
Reverse (-)
7700997 .. 7702454
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0049600.1

Sequence Viewer

Length: 822 bp
ATGAGTGAGCAACTCAAACGAGACTACCAAATATGCGACGAGATCGGCCGGGGTCGATTCGGCGTCGTTTTCAAGGCCGTCAAGAACGGCGACGTCTTCGCCGTCAAGTCCATCGACAAGCGCCTGGCCTCCGGCGACTCCCTGGACGCTCAGTGTCTGTTAACCGAGCCGAAGATCCTCCGGTTGGTGGCTCTCCACCCCAACGTGATCGGCCTCCACGGGTTTTACGAGGACGAAGACCATCTCCACATGGTCCTCGACATGTGTGACGCGCCGGATCTGTTTCGACGCGTCACCCTCAGGTTATTTGCTGAGCCGGAGGCCGCCTCGGTAATGTCACAGCTCATGCAGGCTGTGGCGCACTGTCACCGGCTCGGCGTGGCCCACCGCGATATCAAGCCGGATAACGTGCTGTTCGACGGCTGGGACCGGCTCCTCCTAGCTGACTTTGGATCGGCGGAGACGTTTGGCGAGGGGGAGCTGATGAGCGGCGTGGTCGGGACGCCGTACTACGTGGCGCCGGAGGTCCTTGCCGGGAGGGAGTACGGGGAGAAGGTGGACGTCTGGAGCGCCGGCGTGGTTTTGTACATAATGCTGGCCGGTTTCCCCCCTTTTTACGGCGAGTCTGCGGCGGAGATCTTTGAGGTTGTGCGGAGGGCGAATTTGAGATTTCCGGCGAGGGTTTTCCACTCTGTCTCGCCGTCTGTGAAGGAGTTGTTGAGGAGGATGCTCTGTAAGGACGTTTCTAGAAGATTCTCGGCTGAGCAAGTCCTCAGACACCCATGGATTACAAGTGGGGGGAGGGGCCAGATCGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

273

Amino Acids

30.58

Weight (kDa)

6.0

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 263 5.9e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 11 - 258 1.6e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 96, 564
AccB1I GGYRCC 1 cut(s) 517
AccBSI CCGCTC 1 cut(s) 489
AccII CGCG 3 cut(s) 272, 291, 390
AciI CCGC 7 cut(s) 324, 388, 458, 489, 629, 632, 652
AclWI GGATC 3 cut(s) 169, 285, 460
AcoI YGGCCR 2 cut(s) 46, 597
AcsI RAATTY 1 cut(s) 661
AcyI GRCGYC 5 cut(s) 63, 93, 503, 518, 561
AfaI GTAC 3 cut(s) 509, 545, 587
AfiI CCNNNNNNNGG 3 cut(s) 184, 187, 617
AflIII ACRYGT 2 cut(s) 261, 289
AgsI TTSAA 1 cut(s) 73
AjnI CCWGG 2 cut(s) 123, 141
AluBI AGCT 4 cut(s) 343, 443, 481, 817
AluI AGCT 4 cut(s) 343, 443, 481, 817
Alw26I GTCTC 3 cut(s) 15, 455, 700
AlwI GGATC 3 cut(s) 169, 285, 460
AlwNI CAGNNNCTG 1 cut(s) 157
AoxI GGCC 8 cut(s) 46, 75, 126, 211, 321, 381, 597, 805
ApoI RAATTY 1 cut(s) 661
AspLEI GCGC 5 cut(s) 123, 274, 361, 520, 572
AspS9I GGNCC 5 cut(s) 253, 382, 427, 526, 805
AsuC2I CCSGG 2 cut(s) 50, 535
AsuHPI GGTGA 2 cut(s) 286, 359
AvaII GGWCC 3 cut(s) 253, 427, 526
AxyI CCTNAGG 1 cut(s) 299
BanI GGYRCC 1 cut(s) 517
BbsI GAAGAC 2 cut(s) 88, 243
BccI CCATC 2 cut(s) 119, 249
BceAI ACGGC 7 cut(s) 62, 86, 103, 436, 490, 634, 685
BciT130I CCWGG 2 cut(s) 125, 143
BcnI CCSGG 2 cut(s) 50, 535
BcoDI GTCTC 3 cut(s) 15, 455, 700
BfaI CTAG 2 cut(s) 440, 747
BfoI RGCGCY 3 cut(s) 124, 521, 573
BglII AGATCT 1 cut(s) 636
BisI GCNGC 3 cut(s) 324, 490, 630
BlpI GCTNAGC 2 cut(s) 312, 762
BlsI GCNGC 3 cut(s) 325, 491, 631
Bme1390I CCNGG 4 cut(s) 50, 125, 143, 535
Bme18I GGWCC 3 cut(s) 253, 427, 526
BmgT120I GGNCC 5 cut(s) 253, 382, 427, 526, 805
BmiI GGNNCC 4 cut(s) 428, 434, 519, 806
BmrFI CCNGG 4 cut(s) 50, 125, 143, 535
BmsI GCATC 1 cut(s) 717
BpiI GAAGAC 2 cut(s) 88, 243
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 1 cut(s) 586
Bpu1102I GCTNAGC 2 cut(s) 312, 762
BpuMI CCSGG 2 cut(s) 50, 535
BsaAI YACGTR 1 cut(s) 514
BsaHI GRCGYC 5 cut(s) 63, 93, 503, 518, 561
BsaJI CCNNGG 5 cut(s) 49, 141, 217, 327, 782
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 187, 617
Bse118I RCCGGY 4 cut(s) 369, 429, 572, 599
Bse21I CCTNAGG 1 cut(s) 299
BseBI CCWGG 2 cut(s) 125, 143
BseDI CCNNGG 5 cut(s) 49, 141, 217, 327, 782
BseGI GGATG 1 cut(s) 732
BseLI CCNNNNNNNGG 3 cut(s) 184, 187, 617
BseMII CTCAG 5 cut(s) 164, 303, 313, 753, 787
BseRI GAGGAG 2 cut(s) 425, 736
BseX3I CGGCCG 1 cut(s) 46
BseYI CCCAGC 1 cut(s) 423
Bsh1236I CGCG 3 cut(s) 272, 291, 390
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 8 cut(s) 48, 77, 128, 213, 323, 383, 599, 807
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 440, 514
BslI CCNNNNNNNGG 3 cut(s) 184, 187, 617
BsmAI GTCTC 3 cut(s) 15, 455, 700
BsmBI CGTCTC 1 cut(s) 455
BsmFI GGGAC 2 cut(s) 440, 514
BsnI GGCC 8 cut(s) 48, 77, 128, 213, 323, 383, 599, 807
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
Bsp1720I GCTNAGC 2 cut(s) 312, 762
Bsp19I CCATGG 1 cut(s) 782
BspACI CCGC 7 cut(s) 324, 388, 458, 489, 629, 632, 652
BspANI GGCC 8 cut(s) 48, 77, 128, 213, 323, 383, 599, 807
BspCNI CTCAG 5 cut(s) 163, 304, 312, 754, 786
BspFNI CGCG 3 cut(s) 272, 291, 390
BspLI GGNNCC 4 cut(s) 428, 434, 519, 806
BspPI GGATC 3 cut(s) 169, 285, 460
BspT107I GGYRCC 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 489
BsrFI RCCGGY 4 cut(s) 369, 429, 572, 599
BsrGI TGTACA 1 cut(s) 585
BssAI RCCGGY 4 cut(s) 369, 429, 572, 599
BssECI CCNNGG 5 cut(s) 49, 141, 217, 327, 782
BssMI GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
BssNI GRCGYC 5 cut(s) 63, 93, 503, 518, 561
BssT1I CCWWGG 1 cut(s) 782
Bst2UI CCWGG 2 cut(s) 125, 143
Bst4CI ACNGT 1 cut(s) 365
BstACI GRCGYC 5 cut(s) 63, 93, 503, 518, 561
BstAUI TGTACA 1 cut(s) 585
BstBAI YACGTR 1 cut(s) 514
BstC8I GCNNGC 3 cut(s) 351, 574, 597
BstDEI CTNAG 5 cut(s) 150, 299, 312, 762, 773
BstDSI CCRYGG 2 cut(s) 217, 782
BstF5I GGATG 1 cut(s) 732
BstFNI CGCG 3 cut(s) 272, 291, 390
BstH2I RGCGCY 3 cut(s) 124, 521, 573
BstHHI GCGC 5 cut(s) 123, 274, 361, 520, 572
BstKTI GATC 7 cut(s) 45, 177, 210, 280, 455, 639, 813
BstMAI GTCTC 3 cut(s) 15, 455, 700
BstMBI GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
BstMCI CGRYCG 1 cut(s) 49
BstNI CCWGG 2 cut(s) 125, 143
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 48, 123, 141, 533
BstUI CGCG 3 cut(s) 272, 291, 390
BstV2I GAAGAC 2 cut(s) 88, 243
BstX2I RGATCY 3 cut(s) 174, 277, 636
BstYI RGATCY 3 cut(s) 174, 277, 636
BstZI CGGCCG 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 299
BsuRI GGCC 8 cut(s) 48, 77, 128, 213, 323, 383, 599, 807
BtgI CCRYGG 2 cut(s) 217, 782
BtsCI GGATG 1 cut(s) 732
BtsIMutI CAGTG 2 cut(s) 158, 361
Cac8I GCNNGC 3 cut(s) 351, 574, 597
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 5 cut(s) 123, 274, 361, 520, 572
Cfr10I RCCGGY 4 cut(s) 369, 429, 572, 599
Cfr13I GGNCC 5 cut(s) 253, 382, 427, 526, 805
CseI GACGC 6 cut(s) 52, 155, 278, 280, 297, 511
Csp6I GTAC 3 cut(s) 508, 544, 586
CviAII CATG 4 cut(s) 250, 262, 346, 783
CviQI GTAC 3 cut(s) 508, 544, 586
DdeI CTNAG 5 cut(s) 150, 299, 312, 762, 773
DinI GGCGCC 1 cut(s) 519
DpnI GATC 7 cut(s) 44, 176, 209, 279, 454, 638, 812
DpnII GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
EaeI YGGCCR 2 cut(s) 46, 597
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 2 cut(s) 473, 647
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 782
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 3 cut(s) 253, 427, 526
Eco52I CGGCCG 1 cut(s) 46
Eco81I CCTNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 123, 141
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 782
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 782
Esp3I CGTCTC 1 cut(s) 455
FaeI CATG 4 cut(s) 253, 265, 349, 786
FaiI YATR 6 cut(s) 34, 251, 263, 347, 590, 784
FaqI GGGAC 2 cut(s) 440, 514
FatI CATG 4 cut(s) 249, 261, 345, 782
Fnu4HI GCNGC 3 cut(s) 324, 490, 630
FokI GGATG 1 cut(s) 739
Fsp4HI GCNGC 3 cut(s) 324, 490, 630
FspBI CTAG 2 cut(s) 440, 747
GlaI GCGC 5 cut(s) 122, 273, 360, 519, 571
GluI GCNGC 3 cut(s) 324, 490, 630
GsaI CCCAGC 1 cut(s) 427
GsuI CTGGAG 1 cut(s) 586
HaeII RGCGCY 3 cut(s) 124, 521, 573
HaeIII GGCC 8 cut(s) 48, 77, 128, 213, 323, 383, 599, 807
HgaI GACGC 6 cut(s) 52, 155, 278, 280, 297, 511
HhaI GCGC 5 cut(s) 123, 274, 361, 520, 572
Hin1I GRCGYC 5 cut(s) 63, 93, 503, 518, 561
Hin1II CATG 4 cut(s) 253, 265, 349, 786
Hin6I GCGC 5 cut(s) 121, 272, 359, 518, 570
HinP1I GCGC 5 cut(s) 121, 272, 359, 518, 570
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HinfI GANTC 4 cut(s) 57, 137, 623, 753
HpaI GTTAAC 1 cut(s) 162
HphI GGTGA 2 cut(s) 286, 359
Hpy166II GTNNAC 2 cut(s) 162, 559
Hpy188I TCNGA 1 cut(s) 776
Hpy188III TCNNGA 4 cut(s) 82, 499, 565, 747
Hpy8I GTNNAC 2 cut(s) 162, 559
Hpy99I CGWCG 5 cut(s) 41, 68, 95, 291, 422
HpyAV CCTTC 2 cut(s) 547, 703
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 7 cut(s) 93, 204, 408, 464, 513, 561, 741
HpyCH4V TGCA 1 cut(s) 349
HpyF3I CTNAG 5 cut(s) 150, 299, 312, 762, 773
HpySE526I ACGT 7 cut(s) 93, 204, 408, 464, 513, 561, 741
Hsp92I GRCGYC 5 cut(s) 63, 93, 503, 518, 561
Hsp92II CATG 4 cut(s) 253, 265, 349, 786
HspAI GCGC 5 cut(s) 121, 272, 359, 518, 570
KasI GGCGCC 1 cut(s) 517
KroI GCCGGC 1 cut(s) 572
KroNI GCCGGC 1 cut(s) 574
KspAI GTTAAC 1 cut(s) 162
Kzo9I GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
LmnI GCTCC 3 cut(s) 438, 478, 567
LweI GCATC 1 cut(s) 717
MaeI CTAG 2 cut(s) 440, 747
MaeII ACGT 7 cut(s) 93, 204, 408, 464, 513, 561, 741
MaeIII GTNAC 4 cut(s) 266, 292, 336, 365
MalI GATC 7 cut(s) 44, 176, 209, 279, 454, 638, 812
MbiI CCGCTC 1 cut(s) 489
MboI GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
MboII GAAGA 4 cut(s) 88, 184, 248, 762
MflI RGATCY 3 cut(s) 174, 277, 636
MluCI AATT 1 cut(s) 661
MluI ACGCGT 1 cut(s) 289
Mly113I GGCGCC 1 cut(s) 518
MlyI GAGTC 2 cut(s) 131, 632
MreI CGCCGGCG 1 cut(s) 572
MroNI GCCGGC 1 cut(s) 572
MseI TTAA 1 cut(s) 161
MspR9I CCNGG 4 cut(s) 50, 125, 143, 535
MvaI CCWGG 2 cut(s) 125, 143
MvnI CGCG 3 cut(s) 272, 291, 390
NaeI GCCGGC 1 cut(s) 574
NarI GGCGCC 1 cut(s) 518
NciI CCSGG 2 cut(s) 50, 535
NcoI CCATGG 1 cut(s) 782
NdeII GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
NgoMIV GCCGGC 1 cut(s) 572
NlaIII CATG 4 cut(s) 253, 265, 349, 786
NlaIV GGNNCC 4 cut(s) 428, 434, 519, 806
NmeAIII GCCGAG 2 cut(s) 354, 737
NmuCI GTSAC 4 cut(s) 266, 292, 336, 365
NspI RCATGY 1 cut(s) 265
PciI ACATGT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 225, 414, 461
PdiI GCCGGC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 57, 753
PkrI GCNGC 3 cut(s) 325, 491, 631
PleI GAGTC 2 cut(s) 131, 631
PluTI GGCGCC 1 cut(s) 521
PpsI GAGTC 2 cut(s) 131, 631
Ppu21I YACGTR 1 cut(s) 514
PpuMI RGGWCCY 1 cut(s) 526
PscI ACATGT 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 526
Psp6I CCWGG 2 cut(s) 123, 141
PspFI CCCAGC 1 cut(s) 423
PspGI CCWGG 2 cut(s) 123, 141
PspN4I GGNNCC 4 cut(s) 428, 434, 519, 806
PspPI GGNCC 5 cut(s) 253, 382, 427, 526, 805
PspPPI RGGWCCY 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 3 cut(s) 174, 277, 636
RsaI GTAC 3 cut(s) 509, 545, 587
RsaNI GTAC 3 cut(s) 508, 544, 586
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 3 cut(s) 324, 490, 630
Sau3AI GATC 7 cut(s) 42, 174, 207, 277, 452, 636, 810
Sau96I GGNCC 5 cut(s) 253, 382, 427, 526, 805
SchI GAGTC 2 cut(s) 131, 632
ScrFI CCNGG 4 cut(s) 50, 125, 143, 535
SfaNI GCATC 1 cut(s) 717
SfoI GGCGCC 1 cut(s) 519
SgrAI CRCCGGYG 1 cut(s) 572
SinI GGWCC 3 cut(s) 253, 427, 526
Sse9I AATT 1 cut(s) 661
SsiI CCGC 7 cut(s) 324, 388, 458, 489, 629, 632, 652
SspDI GGCGCC 1 cut(s) 517
SspMI CTAG 2 cut(s) 440, 747
StyD4I CCNGG 4 cut(s) 48, 123, 141, 533
StyI CCWWGG 1 cut(s) 782
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 7 cut(s) 96, 207, 411, 467, 516, 564, 744
TaqI TCGA 6 cut(s) 55, 114, 258, 286, 417, 813
TasI AATT 1 cut(s) 661
TatI WGTACW 1 cut(s) 585
TauI GCSGC 3 cut(s) 326, 492, 632
TfiI GAWTC 2 cut(s) 57, 753
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 2 cut(s) 158, 368
TseFI GTSAC 4 cut(s) 266, 292, 336, 365
Tsp45I GTSAC 4 cut(s) 266, 292, 336, 365
TspRI CASTG 2 cut(s) 158, 368
VpaK11BI GGWCC 3 cut(s) 253, 427, 526
XapI RAATTY 1 cut(s) 661
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 1 cut(s) 265
XspI CTAG 2 cut(s) 440, 747
ZraI GACGTC 2 cut(s) 94, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.