Rroxscaffold_5G00348990

Rare lipoprotein A (RlpA)-like double-psi beta-barrel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001334
Physical Location & Seq
Forward (+)
21568175 .. 21568710
536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348990.1

Sequence Viewer

Length: 378 bp
ATGGGGATTCAGAATATTTTGATTATCTTGAGCATTGTCGCCTGCCTCACGTCGACGTCTATGGCCACACCAGGAACTGCTACTTACTACACGACCTATATTCCATCAGCATGCTACAATTACACCCAAGAAGGTGTGATGATTGCAGCAGCAGGTGACGCCATATGGAACAATGGTGCAGCATGTGGGAAATTTGTCACCGTTAAATGCACAGGTCCTAGGAATGTCGTACCGCCTCCTTGCACCAATGCAAACGTAACCGTAAAAATTATAGATCATTGTCCCGGATGCCCATTTACTCTCGACCTTTCTAAAGAGGCTTTCACCAAAATCGCTAACCCTATGGGGGGAATAATTAATATTGATTATTATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0006950 GO:0008150 GO:0009628 GO:0009987 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0050896 GO:0051716 GO:0070482 GO:0070887 GO:0071453 GO:0071456
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

125

Amino Acids

13.25

Weight (kDa)

6.5

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 49 - 123 3.5e-14 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 53
AciI CCGC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 191
AcyI GRCGYC 2 cut(s) 56, 159
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 346, 347
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 70
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 3 cut(s) 146, 149, 179
ApoI RAATTY 1 cut(s) 191
AseI ATTAAT 1 cut(s) 357
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 167, 190, 316
AvaII GGWCC 1 cut(s) 215
AvrII CCTAGG 1 cut(s) 218
BalI TGGCCA 1 cut(s) 65
BbvI GCAGC 3 cut(s) 158, 161, 191
BccI CCATC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 72
BcnI CCSGG 1 cut(s) 285
BfaI CTAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 147, 150, 180
BlnI CCTAGG 1 cut(s) 218
BlsI GCNGC 3 cut(s) 148, 151, 181
Bme1390I CCNGG 2 cut(s) 72, 285
Bme18I GGWCC 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 2 cut(s) 72, 285
BmsI GCATC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 369
BsaHI GRCGYC 2 cut(s) 56, 159
BsaJI CCNNGG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 347
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 293
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 346, 347
BseXI GCAGC 3 cut(s) 158, 161, 191
BsgI GTGCAG 1 cut(s) 198
BshFI GGCC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 1 cut(s) 267
BslI CCNNNNNNNGG 2 cut(s) 346, 347
BsmFI GGGAC 1 cut(s) 267
BsnI GGCC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 274
BspACI CCGC 1 cut(s) 233
BspANI GGCC 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 1 cut(s) 274
BssNI GRCGYC 2 cut(s) 56, 159
BssT1I CCWWGG 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 202, 262
BstACI GRCGYC 2 cut(s) 56, 159
BstC8I GCNNGC 2 cut(s) 43, 112
BstF5I GGATG 1 cut(s) 293
BstKTI GATC 1 cut(s) 277
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 2 cut(s) 114, 186
BstSCI CCNGG 2 cut(s) 70, 283
BstV1I GCAGC 3 cut(s) 158, 161, 191
BsuRI GGCC 1 cut(s) 65
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 293
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 43, 112
CaiI CAGNNNCTG 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 230
CviAII CATG 2 cut(s) 111, 183
CviJI RGCY 2 cut(s) 65, 320
CviKI_1 RGCY 2 cut(s) 65, 320
CviQI GTAC 1 cut(s) 230
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 2 cut(s) 114, 186
FaiI YATR 8 cut(s) 62, 99, 112, 164, 166, 184, 272, 344
FaqI GGGAC 1 cut(s) 267
FatI CATG 2 cut(s) 110, 182
FauNDI CATATG 1 cut(s) 164
FblI GTMKAC 1 cut(s) 53
Fnu4HI GCNGC 3 cut(s) 147, 150, 180
FokI GGATG 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 147, 150, 180
FspBI CTAG 1 cut(s) 219
GluI GCNGC 3 cut(s) 147, 150, 180
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 285
HgaI GACGC 1 cut(s) 167
Hin1I GRCGYC 2 cut(s) 56, 159
Hin1II CATG 2 cut(s) 114, 186
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 285
HphI GGTGA 3 cut(s) 167, 190, 316
Hpy166II GTNNAC 1 cut(s) 54
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 2 cut(s) 28, 302
Hpy8I GTNNAC 1 cut(s) 54
Hpy99I CGWCG 2 cut(s) 55, 58
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 2 cut(s) 202, 262
HpyCH4IV ACGT 3 cut(s) 50, 56, 255
HpyCH4V TGCA 5 cut(s) 146, 179, 210, 243, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpySE526I ACGT 3 cut(s) 50, 56, 255
Hsp92I GRCGYC 2 cut(s) 56, 159
Hsp92II CATG 2 cut(s) 114, 186
Kzo9I GATC 1 cut(s) 274
LpnPI CCDG 6 cut(s) 55, 57, 84, 138, 198, 298
Lsp1109I GCAGC 3 cut(s) 158, 161, 191
LweI GCATC 1 cut(s) 278
MaeI CTAG 1 cut(s) 219
MaeII ACGT 3 cut(s) 50, 56, 255
MaeIII GTNAC 3 cut(s) 155, 196, 256
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 4 cut(s) 118, 191, 267, 354
MluNI TGGCCA 1 cut(s) 65
MnlI CCTC 3 cut(s) 56, 246, 310
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 2 cut(s) 204, 357
MslI CAYNNNNRTG 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 2 cut(s) 72, 285
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 158
NciI CCSGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 164
NdeII GATC 1 cut(s) 274
NlaIII CATG 2 cut(s) 114, 186
NmuCI GTSAC 2 cut(s) 155, 196
NspI RCATGY 2 cut(s) 114, 186
PaeI GCATGC 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 7
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 3 cut(s) 148, 151, 181
PpuMI RGGWCCY 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspPI GGNCC 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 215
PstNI CAGNNNCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 109
SalI GTCGAC 1 cut(s) 52
SaqAI TTAA 2 cut(s) 204, 357
SatI GCNGC 3 cut(s) 147, 150, 180
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 72, 285
SetI ASST 8 cut(s) 53, 59, 98, 136, 157, 217, 258, 309
SfaNI GCATC 1 cut(s) 278
SgrDI CGTCGACG 1 cut(s) 52
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
SphI GCATGC 1 cut(s) 114
Sse9I AATT 4 cut(s) 118, 191, 267, 354
SsiI CCGC 1 cut(s) 233
SspI AATATT 2 cut(s) 16, 361
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 70, 283
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 2 cut(s) 202, 262
TaiI ACGT 3 cut(s) 53, 59, 258
TaqI TCGA 2 cut(s) 53, 303
TasI AATT 4 cut(s) 118, 191, 267, 354
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 204, 357
Tru9I TTAA 2 cut(s) 204, 357
TseFI GTSAC 2 cut(s) 155, 196
TseI GCWGC 3 cut(s) 146, 149, 179
Tsp45I GTSAC 2 cut(s) 155, 196
VpaK11BI GGWCC 1 cut(s) 215
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 191
XceI RCATGY 2 cut(s) 114, 186
XmaJI CCTAGG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 53
XspI CTAG 1 cut(s) 219
ZraI GACGTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.