Rroxscaffold_5G00349000

EG45-like domain containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001334
Physical Location & Seq
Forward (+)
21597679 .. 21599038
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349000.1

Sequence Viewer

Length: 456 bp
ATGGGAGGAGGGAGTCAGTTTGCTGCATACAAAATATTGCAGAACATCAGCCTAAGTAAAAGTATGGGACTTGTAATGCAGGTGCTGTTTGTGGTGGTGGCCATGGTTGTATGCCTTCCCTTAGCCGCATACGCGGAACACGGAACTGCAACATTTTATACACCTCCTTACGTTCCCTCTGCATGCAATGGGTATGAAAACGACGGTGTGATGATAGCTGCAGCAAGCAGTGCCATTTGGGACAATAAAGGTGCTTGTGGAAGAAGGTACAGAGTAAAATGCACTGGAGCCACAAACCAAGGTGTGCCAAAACCTTGCAAAGATAACAGCGTTGTGGTTAAAATCGTGGATTTCTGTCCTCCTGGATGCCATGGAACCATCGATCTCTCTCAAGAAGCCTTTGCTGCCATCGCTAACCCTGATGCTGGCAAAATCAATATTGAGTTCACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

151

Amino Acids

15.91

Weight (kDa)

6.7

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 73 - 149 2.2e-20 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 70
AccII CGCG 1 cut(s) 134
AciI CCGC 2 cut(s) 126, 134
AcoI YGGCCR 1 cut(s) 99
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 425
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 1 cut(s) 218
AluI AGCT 1 cut(s) 218
AlwNI CAGNNNCTG 1 cut(s) 85
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 4 cut(s) 23, 218, 221, 404
BalI TGGCCA 1 cut(s) 101
BbvI GCAGC 4 cut(s) 10, 205, 233, 391
BccI CCATC 2 cut(s) 386, 416
BciT130I CCWGG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 70
BisI GCNGC 5 cut(s) 24, 126, 219, 222, 405
BlsI GCNGC 5 cut(s) 25, 127, 220, 223, 406
Bme1390I CCNGG 1 cut(s) 363
BmiI GGNNCC 2 cut(s) 289, 376
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 356, 412
BpmI CTGGAG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 102, 298, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 289
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 3 cut(s) 102, 298, 370
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 4 cut(s) 10, 205, 233, 391
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 1 cut(s) 101
BshVI ATCGAT 1 cut(s) 381
BslFI GGGAC 2 cut(s) 81, 254
BslI CCNNNNNNNGG 1 cut(s) 425
BsmFI GGGAC 2 cut(s) 81, 254
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 382
Bsp19I CCATGG 2 cut(s) 102, 370
BspACI CCGC 2 cut(s) 126, 134
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 442
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 2 cut(s) 289, 376
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 193
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 102, 298, 370
BssMI GATC 1 cut(s) 382
BssT1I CCWWGG 3 cut(s) 102, 298, 370
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 3 cut(s) 184, 226, 427
BstDEI CTNAG 3 cut(s) 53, 121, 450
BstDSI CCRYGG 2 cut(s) 102, 370
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 134
BstKTI GATC 1 cut(s) 385
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 4 cut(s) 131, 230, 404, 410
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 4 cut(s) 10, 205, 233, 391
Bsu15I ATCGAT 1 cut(s) 381
BsuRI GGCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 2 cut(s) 102, 370
BtgZI GCGATG 1 cut(s) 394
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 235, 282, 447
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 3 cut(s) 184, 226, 427
CaiI CAGNNNCTG 1 cut(s) 85
ClaI ATCGAT 1 cut(s) 381
Csp6I GTAC 1 cut(s) 268
CviAII CATG 3 cut(s) 103, 183, 371
CviJI RGCY 6 cut(s) 51, 101, 125, 218, 290, 398
CviKI_1 RGCY 6 cut(s) 51, 101, 125, 218, 290, 398
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 53, 121, 450
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 99
Eco130I CCWWGG 3 cut(s) 102, 298, 370
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 3 cut(s) 102, 298, 370
ErhI CCWWGG 3 cut(s) 102, 298, 370
FaeI CATG 3 cut(s) 106, 186, 374
FaiI YATR 9 cut(s) 28, 65, 104, 112, 130, 159, 184, 195, 372
FaqI GGGAC 2 cut(s) 81, 254
FatI CATG 3 cut(s) 102, 182, 370
Fnu4HI GCNGC 5 cut(s) 24, 126, 219, 222, 405
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 5 cut(s) 24, 126, 219, 222, 405
GluI GCNGC 5 cut(s) 24, 126, 219, 222, 405
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 1 cut(s) 101
Hin1II CATG 3 cut(s) 106, 186, 374
HinfI GANTC 1 cut(s) 13
Hpy166II GTNNAC 1 cut(s) 447
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 1 cut(s) 447
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 125, 258
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 9 cut(s) 26, 40, 79, 149, 182, 186, 221, 282, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 230, 404, 410
HpyF3I CTNAG 3 cut(s) 53, 121, 450
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 3 cut(s) 106, 186, 374
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 6 cut(s) 65, 270, 348, 375, 411, 432
Lsp1109I GCAGC 4 cut(s) 10, 205, 233, 391
LweI GCATC 2 cut(s) 356, 412
MaeII ACGT 1 cut(s) 171
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 1 cut(s) 273
MlsI TGGCCA 1 cut(s) 101
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 3 cut(s) 174, 187, 369
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 4 cut(s) 131, 230, 404, 410
NcoI CCATGG 2 cut(s) 102, 370
NdeII GATC 1 cut(s) 382
NlaIII CATG 3 cut(s) 106, 186, 374
NlaIV GGNNCC 2 cut(s) 289, 376
NspI RCATGY 1 cut(s) 186
PaeI GCATGC 1 cut(s) 186
PaqCI CACCTGC 1 cut(s) 70
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 5 cut(s) 25, 127, 220, 223, 406
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 289, 376
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 85
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 5 cut(s) 24, 126, 219, 222, 405
Sau3AI GATC 1 cut(s) 382
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 363
SetI ASST 8 cut(s) 84, 166, 174, 220, 253, 269, 304, 316
SfaNI GCATC 2 cut(s) 356, 412
SfcI CTRYAG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 390
SmoI CTYRAG 1 cut(s) 390
SphI GCATGC 1 cut(s) 186
SsiI CCGC 2 cut(s) 126, 134
SspI AATATT 2 cut(s) 36, 439
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 3 cut(s) 102, 298, 370
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 381
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 3 cut(s) 235, 289, 454
TseI GCWGC 4 cut(s) 23, 218, 221, 404
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 3 cut(s) 235, 289, 454
XceI RCATGY 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.