pycom05g18840

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002250
Physical Location & Seq
Forward (+)
21679156 .. 21681366
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1179 bp
ATGATATTGTTTGTTCAAAAAAAGTGGCACTCTCCATCAATTCTCTTTGCATCTCCCAAAAAAAAAAAAAACCAAACTGCAATTAAAAAAAAAAAAGGTAAAAAGCTCAACGCCCACTGGGAACCCGAAGAGGAAGAAATGACCCGATTTCCACCTCCATCTCCTCCTCCTTCTTCTTCATCTTCGCTATTGATTCCGATCGACGAATCCCTAACCCTAATTCCCGGCCTCCCAAACGACGTCGCTGAGCTGATCCTCTCCTTCATCCCCTTCTCCCACCAGGCCCGTCTCCGACCCATCTGCAAATCATGGAGGCTCTTCCTCTCCTCCAAAACCCTAATCGCCCTCCGCCAGACCCACCACCGCAGCAACCTTTCCCACCTCCTCTGCATCTTCCCCCAGGACCCCTCCGTCGCCGCCCCTTACCTCTTCGATCCCCGAACCCTAGCCTGGTGCCCCCTCCCGCCCATGCCCTGCAACCCCCACGTCTACGGCCTCTGCAACTTCACCTCCCTCTCCATTGGGCCCCATCTCTACGTCATCGGCGGCTCGCTCTTCGACACTCGTTCGTTTCCGATCGACCGCCCCTCGCCGTCCTCCGCGGTCTTCCGATTTGACTTCAGCACTTCCTCGTGGGACTTCCTCGCTCCGATGCTCACGCCGCGTGGAAGTTTTGCCTGCGCCGCAGTTCCAAATTCGGGAGAGATTCTCGTCGCTGGCGGCGGATCCCGGCACACTCTGTTCAGCGCGGCTGGAAGCCGAATGAGCTCAGTCGAGCGGTATGACATTGGTAGAAATGAATGGGTTGCAATGGACGGGTTGCCAGGTTTTCGGGCTGGGTGCGCGGGGTTCTTTGTTGGGGATGGCGAGGAGAGGGAGTTCTGGGTTATGGGTGGGTACGGCGAGTCCAGAACAATATCAGGGCTTTTTCCGGTGGACGAGCATTACAGAGATGCCGTGGTAATGGAATTGAAGAATGGGAATGGTGGTTGCAGGTGGCGAGAGGTTGGAGATATGTGGGAAGCAGGGGAGAGGATGAGACTGGGCAAAACTGTTGTCGTGGAAGATGGCGATGATCGCGGTCGACCTGCTGTTTTCATGCTTGATCGGAATGTTATATTCAGGTTGAAAGTTCTCTCTATTTTTCACCACAAGGAGAATGCAGAAGACAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

393

Amino Acids

43.76

Weight (kDa)

8.73

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_1 PF01344 167 - 218 1.3e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 984
AasI GACNNNNNNGTC 1 cut(s) 410
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 2 cut(s) 984, 1096
AccB1I GGYRCC 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 778
AccI GTMKAC 2 cut(s) 489, 1084
AccII CGCG 5 cut(s) 602, 664, 749, 845, 1080
AclWI GGATC 4 cut(s) 247, 428, 720, 733
AcsI RAATTY 1 cut(s) 694
AcuI CTGAAG 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 2 cut(s) 450, 698
AgsI TTSAA 3 cut(s) 17, 973, 1129
AjiI CACGTC 1 cut(s) 487
AjnI CCWGG 4 cut(s) 279, 399, 449, 823
AloI GAACNNNNNNTCC 2 cut(s) 863, 895
AluBI AGCT 4 cut(s) 106, 250, 768, 1175
AluI AGCT 4 cut(s) 106, 250, 768, 1175
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 2 cut(s) 293, 1033
AlwI GGATC 4 cut(s) 247, 428, 720, 733
AoxI GGCC 4 cut(s) 226, 282, 493, 524
ApaI GGGCCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 694
ArsI GACNNNNNNTTYG 2 cut(s) 1041, 1073
AspLEI GCGC 3 cut(s) 683, 749, 845
AspS9I GGNCC 4 cut(s) 283, 403, 524, 525
AsuC2I CCSGG 2 cut(s) 225, 730
AsuHPI GGTGA 2 cut(s) 499, 1139
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 2 cut(s) 458, 528
BamHI GGATCC 1 cut(s) 725
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 2 cut(s) 528, 770
BauI CACGAG 1 cut(s) 631
BbsI GAAGAC 2 cut(s) 598, 1173
Bbv12I GWGCWC 1 cut(s) 770
BbvI GCAGC 1 cut(s) 378
BccI CCATC 6 cut(s) 43, 166, 305, 537, 857, 1061
BceAI ACGGC 4 cut(s) 508, 577, 916, 941
BcgI CGANNNNNNTGC 2 cut(s) 282, 316
BciT130I CCWGG 4 cut(s) 281, 401, 451, 825
BcnI CCSGG 2 cut(s) 225, 730
BcoDI GTCTC 2 cut(s) 293, 1033
BfaI CTAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 984, 1096
BisI GCNGC 7 cut(s) 367, 417, 547, 662, 684, 721, 750
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 7 cut(s) 368, 418, 548, 663, 685, 722, 751
Bme1390I CCNGG 6 cut(s) 225, 281, 401, 451, 730, 825
Bme18I GGWCC 1 cut(s) 403
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 4 cut(s) 283, 403, 524, 525
BmiI GGNNCC 6 cut(s) 123, 405, 455, 526, 527, 727
BmrFI CCNGG 6 cut(s) 225, 281, 401, 451, 730, 825
BmrI ACTGGG 2 cut(s) 127, 1052
BmsI GCATC 4 cut(s) 59, 399, 642, 943
BmuI ACTGGG 2 cut(s) 127, 1052
BpiI GAAGAC 2 cut(s) 598, 1173
BplI GAGNNNNNCTC 2 cut(s) 693, 725
Bpu1102I GCTNAGC 1 cut(s) 246
BpuMI CCSGG 2 cut(s) 225, 730
BsaBI GATNNNNATC 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 399, 600, 957
BsaWI WCCGGW 1 cut(s) 931
BsaXI ACNNNNNCTCC 4 cut(s) 494, 524, 863, 893
Bsc4I CCNNNNNNNGG 2 cut(s) 450, 698
Bse1I ACTGG 2 cut(s) 122, 1047
Bse3DI GCAATG 1 cut(s) 816
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 4 cut(s) 281, 401, 451, 825
BseDI CCNNGG 3 cut(s) 399, 600, 957
BseGI GGATG 3 cut(s) 264, 868, 1041
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 450, 698
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 2 cut(s) 237, 783
BseNI ACTGG 2 cut(s) 122, 1047
BseRI GAGGAG 5 cut(s) 153, 156, 316, 374, 884
BseSI GKGCMC 2 cut(s) 458, 528
BseXI GCAGC 1 cut(s) 378
BseYI CCCAGC 1 cut(s) 836
Bsh1236I CGCG 5 cut(s) 602, 664, 749, 845, 1080
Bsh1285I CGRYCG 4 cut(s) 201, 579, 583, 1084
BshFI GGCC 4 cut(s) 228, 284, 495, 526
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 4 cut(s) 201, 579, 583, 1084
BsiHKAI GWGCWC 1 cut(s) 770
BsiSI CCGG 3 cut(s) 225, 730, 932
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 450, 698
BsmAI GTCTC 2 cut(s) 293, 1033
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 4 cut(s) 228, 284, 495, 526
Bsp120I GGGCCC 1 cut(s) 524
Bsp1286I GDGCHC 3 cut(s) 458, 528, 770
Bsp143I GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
Bsp1720I GCTNAGC 1 cut(s) 246
BspANI GGCC 4 cut(s) 228, 284, 495, 526
BspCNI CTCAG 2 cut(s) 238, 782
BspFNI CGCG 5 cut(s) 602, 664, 749, 845, 1080
BspLI GGNNCC 6 cut(s) 123, 405, 455, 526, 527, 727
BspMI ACCTGC 2 cut(s) 984, 1096
BspPI GGATC 4 cut(s) 247, 428, 720, 733
BspQI GCTCTTC 2 cut(s) 323, 560
BspT107I GGYRCC 1 cut(s) 453
BsrBI CCGCTC 1 cut(s) 778
BsrDI GCAATG 1 cut(s) 816
BsrI ACTGG 2 cut(s) 122, 1047
BssECI CCNNGG 3 cut(s) 399, 600, 957
BssMI GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 631
Bst2BI CACGAG 1 cut(s) 631
Bst2UI CCWGG 4 cut(s) 281, 401, 451, 825
Bst4CI ACNGT 1 cut(s) 1054
Bst6I CTCTTC 4 cut(s) 123, 323, 434, 560
BstACI GRCGYC 1 cut(s) 240
BstC8I GCNNGC 3 cut(s) 551, 679, 718
BstDEI CTNAG 2 cut(s) 246, 769
BstDSI CCRYGG 2 cut(s) 600, 957
BstF5I GGATG 3 cut(s) 264, 868, 1041
BstFNI CGCG 5 cut(s) 602, 664, 749, 845, 1080
BstHHI GCGC 3 cut(s) 683, 749, 845
BstKTI GATC 7 cut(s) 201, 255, 436, 579, 728, 1078, 1108
BstMAI GTCTC 2 cut(s) 293, 1033
BstMBI GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
BstMCI CGRYCG 4 cut(s) 201, 579, 583, 1084
BstMWI GCNNNNNNNGC 5 cut(s) 661, 683, 765, 842, 1077
BstNI CCWGG 4 cut(s) 281, 401, 451, 825
BstSCI CCNGG 6 cut(s) 223, 279, 399, 449, 728, 823
BstSLI GKGCMC 2 cut(s) 458, 528
BstUI CGCG 5 cut(s) 602, 664, 749, 845, 1080
BstV1I GCAGC 1 cut(s) 378
BstV2I GAAGAC 2 cut(s) 598, 1173
BstX2I RGATCY 1 cut(s) 725
BstYI RGATCY 1 cut(s) 725
BsuRI GGCC 4 cut(s) 228, 284, 495, 526
BtgI CCRYGG 2 cut(s) 600, 957
BtgZI GCGATG 1 cut(s) 1086
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 3 cut(s) 264, 868, 1041
BtsIMutI CAGTG 1 cut(s) 115
BveI ACCTGC 2 cut(s) 984, 1096
Cac8I GCNNGC 3 cut(s) 551, 679, 718
CfoI GCGC 3 cut(s) 683, 749, 845
Cfr13I GGNCC 4 cut(s) 283, 403, 524, 525
Cfr42I CCGCGG 1 cut(s) 603
Csp6I GTAC 1 cut(s) 898
CspCI CAANNNNNGTGG 2 cut(s) 5, 40
CviAII CATG 3 cut(s) 309, 469, 1099
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 2 cut(s) 246, 769
DpnI GATC 7 cut(s) 200, 254, 435, 578, 727, 1077, 1107
DpnII GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 4 cut(s) 123, 323, 434, 560
EarI CTCTTC 4 cut(s) 123, 323, 434, 560
EciI GGCGGA 2 cut(s) 338, 738
Ecl136II GAGCTC 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 528, 770
Eco47I GGWCC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 768
Eco57I CTGAAG 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 768
EcoO109I RGGNCCY 2 cut(s) 403, 525
EcoRII CCWGG 4 cut(s) 279, 399, 449, 823
EcoT38I GRGCYC 2 cut(s) 528, 770
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 3 cut(s) 312, 472, 1102
FaiI YATR 7 cut(s) 310, 470, 783, 890, 1016, 1100, 1118
FaqI GGGAC 1 cut(s) 650
FatI CATG 3 cut(s) 308, 468, 1098
FauI CCCGC 2 cut(s) 471, 838
FblI GTMKAC 2 cut(s) 489, 1084
Fnu4HI GCNGC 7 cut(s) 367, 417, 547, 662, 684, 721, 750
FokI GGATG 3 cut(s) 251, 875, 1048
FriOI GRGCYC 2 cut(s) 528, 770
Fsp4HI GCNGC 7 cut(s) 367, 417, 547, 662, 684, 721, 750
FspBI CTAG 1 cut(s) 446
GlaI GCGC 3 cut(s) 682, 748, 844
GluI GCNGC 7 cut(s) 367, 417, 547, 662, 684, 721, 750
GsaI CCCAGC 1 cut(s) 840
HaeIII GGCC 4 cut(s) 228, 284, 495, 526
HapII CCGG 3 cut(s) 225, 730, 932
HhaI GCGC 3 cut(s) 683, 749, 845
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 3 cut(s) 312, 472, 1102
Hin6I GCGC 3 cut(s) 681, 747, 843
HinP1I GCGC 3 cut(s) 681, 747, 843
HincII GTYRAC 1 cut(s) 1085
HindII GTYRAC 1 cut(s) 1085
HinfI GANTC 4 cut(s) 193, 206, 706, 905
HpaII CCGG 3 cut(s) 225, 730, 932
HphI GGTGA 2 cut(s) 499, 1139
Hpy166II GTNNAC 3 cut(s) 490, 937, 1085
Hpy188I TCNGA 6 cut(s) 198, 293, 576, 611, 651, 1110
Hpy188III TCNNGA 2 cut(s) 699, 909
Hpy8I GTNNAC 3 cut(s) 490, 937, 1085
Hpy99I CGWCG 5 cut(s) 206, 242, 245, 416, 716
HpyAV CCTTC 3 cut(s) 180, 271, 280
HpyCH4III ACNGT 1 cut(s) 1054
HpyCH4IV ACGT 3 cut(s) 240, 486, 537
HpyCH4V TGCA 9 cut(s) 50, 80, 303, 390, 477, 501, 809, 993, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 661, 683, 765, 842, 1077
HpyF3I CTNAG 2 cut(s) 246, 769
HpySE526I ACGT 3 cut(s) 240, 486, 537
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 3 cut(s) 312, 472, 1102
HspAI GCGC 3 cut(s) 681, 747, 843
KspI CCGCGG 1 cut(s) 603
Kzo9I GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
LguI GCTCTTC 2 cut(s) 323, 560
LmnI GCTCC 1 cut(s) 652
Lsp1109I GCAGC 1 cut(s) 378
LweI GCATC 4 cut(s) 59, 399, 642, 943
MaeI CTAG 1 cut(s) 446
MaeII ACGT 3 cut(s) 240, 486, 537
MalI GATC 7 cut(s) 200, 254, 435, 578, 727, 1077, 1107
MbiI CCGCTC 1 cut(s) 778
MboI GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
MflI RGATCY 1 cut(s) 725
MhlI GDGCHC 3 cut(s) 458, 528, 770
MluCI AATT 5 cut(s) 39, 81, 219, 694, 968
MlyI GAGTC 1 cut(s) 914
MmeI TCCRAC 2 cut(s) 316, 988
MseI TTAA 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 3 cut(s) 225, 730, 932
MspR9I CCNGG 6 cut(s) 225, 281, 401, 451, 730, 825
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 4 cut(s) 281, 401, 451, 825
MvnI CGCG 5 cut(s) 602, 664, 749, 845, 1080
MwoI GCNNNNNNNGC 5 cut(s) 661, 683, 765, 842, 1077
NciI CCSGG 2 cut(s) 225, 730
NdeII GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
NlaIII CATG 3 cut(s) 312, 472, 1102
NlaIV GGNNCC 6 cut(s) 123, 405, 455, 526, 527, 727
PaqCI CACCTGC 1 cut(s) 984
PciSI GCTCTTC 2 cut(s) 323, 560
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 3 cut(s) 193, 206, 706
PkrI GCNGC 7 cut(s) 368, 418, 548, 663, 685, 722, 751
Ple19I CGATCG 2 cut(s) 201, 579
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
PpuMI RGGWCCY 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 770
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 4 cut(s) 279, 399, 449, 823
PspFI CCCAGC 1 cut(s) 836
PspGI CCWGG 4 cut(s) 279, 399, 449, 823
PspN4I GGNNCC 6 cut(s) 123, 405, 455, 526, 527, 727
PspOMI GGGCCC 1 cut(s) 524
PspPI GGNCC 4 cut(s) 283, 403, 524, 525
PspPPI RGGWCCY 1 cut(s) 403
PsuI RGATCY 1 cut(s) 725
PvuI CGATCG 2 cut(s) 201, 579
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
SacI GAGCTC 1 cut(s) 770
SacII CCGCGG 1 cut(s) 603
SalI GTCGAC 1 cut(s) 1083
SapI GCTCTTC 2 cut(s) 323, 560
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 7 cut(s) 367, 417, 547, 662, 684, 721, 750
Sau3AI GATC 7 cut(s) 198, 252, 433, 576, 725, 1075, 1105
Sau96I GGNCC 4 cut(s) 283, 403, 524, 525
SchI GAGTC 1 cut(s) 914
ScrFI CCNGG 6 cut(s) 225, 281, 401, 451, 730, 825
SduI GDGCHC 3 cut(s) 458, 528, 770
SfaNI GCATC 4 cut(s) 59, 399, 642, 943
Sfr303I CCGCGG 1 cut(s) 603
SgrBI CCGCGG 1 cut(s) 603
SinI GGWCC 1 cut(s) 403
Sse9I AATT 5 cut(s) 39, 81, 219, 694, 968
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 770
StyD4I CCNGG 6 cut(s) 223, 279, 399, 449, 728, 823
TaaI ACNGT 1 cut(s) 1054
TaiI ACGT 3 cut(s) 243, 489, 540
TaqI TCGA 6 cut(s) 201, 432, 558, 579, 774, 1084
TasI AATT 5 cut(s) 39, 81, 219, 694, 968
TauI GCSGC 6 cut(s) 419, 549, 664, 686, 723, 752
TfiI GAWTC 3 cut(s) 193, 206, 706
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 122
TseI GCWGC 1 cut(s) 366
TspDTI ATGAA 4 cut(s) 168, 253, 813, 1087
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 1 cut(s) 694
XmiI GTMKAC 2 cut(s) 489, 1084
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.